Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Neurotoxicity of acrylamide - (reply: 2)
32. 2 bands very close in the expected place - (reply: 2)
33. Pressing Too Hard on Transfer Sandwich - (reply: 3)
34. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
35. SDS-PAGE High MW bands dissapear! - (reply: 7)
36. Non-specific binding of protein in western blot - (reply: 3)
37. SDS-PAGE vertical lane streaking - (reply: 1)
38. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
39. Storing membrane after antibody incubation - (reply: 2)
40. layer in between resolving and stacking gel - (reply: 1)
41. Lane spillover (?) on Western Blot - (reply: 3)
42. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
43. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
44. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
45. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
46. No band detected in WB - (reply: 4)
47. Concentration of Sample Buffer to boil protein samples - (reply: 2)
48. Western Blot diffuse bands - (reply: 3)
49. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
50. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
51. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
52. Some bands transferred, some not..? Why??? - (reply: 1)
53. Digital imager questions on correction - (reply: 4)
54. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
55. Rapidly decomposing HRP substrate - (reply: 2)
56. protein size in SDS-PAGE - (reply: 4)
57. Protein not migrating through gel - (reply: 9)
58. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
59. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
60. Extraction of bands from SDS-PAGE - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37