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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Protein not migrating through gel - (reply: 9)
32. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
33. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
34. Extraction of bands from SDS-PAGE - (reply: 2)
35. Dumbbell-shaped bands on the WB - (reply: 3)
36. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
37. Deformed bands in gel - (reply: 8)
38. non specific immunoblot - (reply: 2)
39. Protein stuck in the origin of resolving gel - (reply: 12)
40. detection of small peptide by the SDS-PAGE gel - (reply: 1)
41. Help in western blotting GAPDH bands are not even - (reply: 1)
42. 37kb and 7kb protein in western blot - (reply: 24)
43. Dot blot/Western background signal problem - (reply: 1)
44. Blocking and Washing membrane - (reply: 3)
45. detection of a protein band of protein complex - (reply: 3)
46. Cell lysis - (reply: 1)
47. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
48. Western blot detects exogenous protein but not endogenous - (reply: 2)
49. White bands in western blotting - (reply: 4)
50. Question with the molecular weight of my protein - (reply: 1)
51. Possible Protein Aggregation Riddle - (reply: 3)
52. How to obtain a control positive for my WB - (reply: 3)
53. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
54. Antibody not binding to low MW targets - (reply: 4)
55. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
56. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
57. Question on loss of signal between samples in western blot - (reply: 4)
58. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
59. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
60. Antibody Issues - (reply: 6)

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