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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Digital imager questions on correction - (reply: 4)
32. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
33. Rapidly decomposing HRP substrate - (reply: 2)
34. protein size in SDS-PAGE - (reply: 4)
35. Protein not migrating through gel - (reply: 9)
36. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
37. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
38. Extraction of bands from SDS-PAGE - (reply: 2)
39. Dumbbell-shaped bands on the WB - (reply: 3)
40. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
41. Deformed bands in gel - (reply: 8)
42. non specific immunoblot - (reply: 2)
43. Protein stuck in the origin of resolving gel - (reply: 12)
44. detection of small peptide by the SDS-PAGE gel - (reply: 1)
45. Help in western blotting GAPDH bands are not even - (reply: 1)
46. 37kb and 7kb protein in western blot - (reply: 24)
47. Dot blot/Western background signal problem - (reply: 1)
48. Blocking and Washing membrane - (reply: 3)
49. detection of a protein band of protein complex - (reply: 3)
50. Cell lysis - (reply: 1)
51. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
52. Western blot detects exogenous protein but not endogenous - (reply: 2)
53. White bands in western blotting - (reply: 4)
54. Question with the molecular weight of my protein - (reply: 1)
55. Possible Protein Aggregation Riddle - (reply: 3)
56. How to obtain a control positive for my WB - (reply: 3)
57. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
58. Antibody not binding to low MW targets - (reply: 4)
59. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
60. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)

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