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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
32. Blocking agent in western blotting of histone proteins - (reply: 1)
33. Best western loading control for comparing different cell lines - (reply: 2)
34. western blotting running problem? - (reply: 2)
35. Keeping apoptotic/detached cells for WB? - (reply: 3)
36. Advice with WB please - (reply: 3)
37. Western Blot Wet Transfer Conditions - (reply: 10)
38. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
39. Western Blot and Inmunofluorescence contradictions - (reply: 12)
40. Nanog double band in WB - (reply: 1)
41. My beta actin bands are changing - (reply: 6)
42. ECL Substrate Choices - (reply: 2)
43. Why does my gel have streaks instead of bands? - (reply: 2)
44. Left ECL kit at room temp. Is it ruined? - (reply: 2)
45. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
46. Native PAGE problem - not entering gel enough - (reply: 9)
47. film saturation in immunoblots - (reply: 1)
48. Problems with wells on SDS PAGE gels - (reply: 3)
49. Protein is present, but not detected by antibody - (reply: 4)
50. secondary antibody creation - (reply: 1)
51. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
52. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
53. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
54. Low signal, weird background stains on blot - (reply: 5)
55. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
56. Incomplete Blotting and staining of NC membrane - (reply: 7)
57. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
58. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
59. Help, protein ladder didn`t separate - (reply: 3)
60. Determining phosphorylation status of a protein by transfection - (reply: 5)

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