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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
32. Storing membrane after antibody incubation - (reply: 2)
33. layer in between resolving and stacking gel - (reply: 1)
34. Lane spillover (?) on Western Blot - (reply: 3)
35. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
36. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
37. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
38. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
39. No band detected in WB - (reply: 4)
40. Concentration of Sample Buffer to boil protein samples - (reply: 2)
41. Western Blot diffuse bands - (reply: 3)
42. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
43. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
44. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
45. Some bands transferred, some not..? Why??? - (reply: 1)
46. Digital imager questions on correction - (reply: 4)
47. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
48. Rapidly decomposing HRP substrate - (reply: 2)
49. protein size in SDS-PAGE - (reply: 4)
50. Protein not migrating through gel - (reply: 9)
51. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
52. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
53. Extraction of bands from SDS-PAGE - (reply: 2)
54. Dumbbell-shaped bands on the WB - (reply: 3)
55. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
56. Deformed bands in gel - (reply: 8)
57. non specific immunoblot - (reply: 2)
58. Protein stuck in the origin of resolving gel - (reply: 12)
59. detection of small peptide by the SDS-PAGE gel - (reply: 1)
60. Help in western blotting GAPDH bands are not even - (reply: 1)

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