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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
32. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
33. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
34. Some bands transferred, some not..? Why??? - (reply: 1)
35. Digital imager questions on correction - (reply: 4)
36. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
37. Rapidly decomposing HRP substrate - (reply: 2)
38. protein size in SDS-PAGE - (reply: 4)
39. Protein not migrating through gel - (reply: 9)
40. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
41. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
42. Extraction of bands from SDS-PAGE - (reply: 2)
43. Dumbbell-shaped bands on the WB - (reply: 3)
44. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
45. Deformed bands in gel - (reply: 8)
46. non specific immunoblot - (reply: 2)
47. Protein stuck in the origin of resolving gel - (reply: 12)
48. detection of small peptide by the SDS-PAGE gel - (reply: 1)
49. Help in western blotting GAPDH bands are not even - (reply: 1)
50. 37kb and 7kb protein in western blot - (reply: 24)
51. Dot blot/Western background signal problem - (reply: 1)
52. Blocking and Washing membrane - (reply: 3)
53. detection of a protein band of protein complex - (reply: 3)
54. Cell lysis - (reply: 1)
55. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
56. Western blot detects exogenous protein but not endogenous - (reply: 2)
57. White bands in western blotting - (reply: 4)
58. Question with the molecular weight of my protein - (reply: 1)
59. Possible Protein Aggregation Riddle - (reply: 3)
60. How to obtain a control positive for my WB - (reply: 3)

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