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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Non-specific binding of protein in western blot - (reply: 3)
32. SDS-PAGE vertical lane streaking - (reply: 1)
33. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
34. Storing membrane after antibody incubation - (reply: 2)
35. layer in between resolving and stacking gel - (reply: 1)
36. Lane spillover (?) on Western Blot - (reply: 3)
37. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
38. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
39. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
40. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
41. No band detected in WB - (reply: 4)
42. Concentration of Sample Buffer to boil protein samples - (reply: 2)
43. Western Blot diffuse bands - (reply: 3)
44. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
45. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
46. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
47. Some bands transferred, some not..? Why??? - (reply: 1)
48. Digital imager questions on correction - (reply: 4)
49. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
50. Rapidly decomposing HRP substrate - (reply: 2)
51. protein size in SDS-PAGE - (reply: 4)
52. Protein not migrating through gel - (reply: 9)
53. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
54. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
55. Extraction of bands from SDS-PAGE - (reply: 2)
56. Dumbbell-shaped bands on the WB - (reply: 3)
57. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
58. Deformed bands in gel - (reply: 8)
59. non specific immunoblot - (reply: 2)
60. Protein stuck in the origin of resolving gel - (reply: 12)

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