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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Rapidly decomposing HRP substrate - (reply: 2)
32. protein size in SDS-PAGE - (reply: 4)
33. Protein not migrating through gel - (reply: 9)
34. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
35. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
36. Extraction of bands from SDS-PAGE - (reply: 2)
37. Dumbbell-shaped bands on the WB - (reply: 3)
38. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
39. Deformed bands in gel - (reply: 8)
40. non specific immunoblot - (reply: 2)
41. Protein stuck in the origin of resolving gel - (reply: 12)
42. detection of small peptide by the SDS-PAGE gel - (reply: 1)
43. Help in western blotting GAPDH bands are not even - (reply: 1)
44. 37kb and 7kb protein in western blot - (reply: 24)
45. Dot blot/Western background signal problem - (reply: 1)
46. Blocking and Washing membrane - (reply: 3)
47. detection of a protein band of protein complex - (reply: 3)
48. Cell lysis - (reply: 1)
49. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
50. Western blot detects exogenous protein but not endogenous - (reply: 2)
51. White bands in western blotting - (reply: 4)
52. Question with the molecular weight of my protein - (reply: 1)
53. Possible Protein Aggregation Riddle - (reply: 3)
54. How to obtain a control positive for my WB - (reply: 3)
55. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
56. Antibody not binding to low MW targets - (reply: 4)
57. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
58. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
59. Question on loss of signal between samples in western blot - (reply: 4)
60. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)

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