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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. problem of cell lysate - (reply: 3)
32. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
33. Blocking agent in western blotting of histone proteins - (reply: 1)
34. Best western loading control for comparing different cell lines - (reply: 2)
35. western blotting running problem? - (reply: 2)
36. Keeping apoptotic/detached cells for WB? - (reply: 3)
37. Advice with WB please - (reply: 3)
38. Western Blot Wet Transfer Conditions - (reply: 10)
39. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
40. Western Blot and Inmunofluorescence contradictions - (reply: 12)
41. Nanog double band in WB - (reply: 1)
42. My beta actin bands are changing - (reply: 6)
43. ECL Substrate Choices - (reply: 2)
44. Why does my gel have streaks instead of bands? - (reply: 2)
45. Left ECL kit at room temp. Is it ruined? - (reply: 2)
46. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
47. Native PAGE problem - not entering gel enough - (reply: 9)
48. film saturation in immunoblots - (reply: 1)
49. Problems with wells on SDS PAGE gels - (reply: 3)
50. Protein is present, but not detected by antibody - (reply: 4)
51. secondary antibody creation - (reply: 1)
52. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
53. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
54. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
55. Low signal, weird background stains on blot - (reply: 5)
56. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
57. Incomplete Blotting and staining of NC membrane - (reply: 7)
58. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
59. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
60. Help, protein ladder didn`t separate - (reply: 3)

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