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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Western Blot Wet Transfer Conditions - (reply: 10)
32. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
33. Western Blot and Inmunofluorescence contradictions - (reply: 12)
34. Nanog double band in WB - (reply: 1)
35. My beta actin bands are changing - (reply: 6)
36. ECL Substrate Choices - (reply: 2)
37. Why does my gel have streaks instead of bands? - (reply: 2)
38. Left ECL kit at room temp. Is it ruined? - (reply: 2)
39. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
40. Native PAGE problem - not entering gel enough - (reply: 9)
41. film saturation in immunoblots - (reply: 1)
42. Problems with wells on SDS PAGE gels - (reply: 3)
43. Protein is present, but not detected by antibody - (reply: 4)
44. secondary antibody creation - (reply: 1)
45. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
46. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
47. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
48. Low signal, weird background stains on blot - (reply: 5)
49. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
50. Incomplete Blotting and staining of NC membrane - (reply: 7)
51. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
52. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
53. Help, protein ladder didn`t separate - (reply: 3)
54. Determining phosphorylation status of a protein by transfection - (reply: 5)
55. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
56. Problem with Denative lysis - (reply: 1)
57. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
58. Polyclonal antibody precipitating - (reply: 1)
59. manual 2D electrophoresis - (reply: 8)
60. relationship between protein size, lysate concentration, and band intensity - (reply: 3)

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