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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Blocking and Washing membrane - (reply: 3)
32. detection of a protein band of protein complex - (reply: 3)
33. Cell lysis - (reply: 1)
34. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
35. Western blot detects exogenous protein but not endogenous - (reply: 2)
36. White bands in western blotting - (reply: 4)
37. Question with the molecular weight of my protein - (reply: 1)
38. Possible Protein Aggregation Riddle - (reply: 3)
39. How to obtain a control positive for my WB - (reply: 3)
40. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
41. Antibody not binding to low MW targets - (reply: 4)
42. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
43. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
44. Question on loss of signal between samples in western blot - (reply: 4)
45. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
46. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
47. Antibody Issues - (reply: 6)
48. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
49. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
50. Is it possible to "over-block" a membrane? - (reply: 5)
51. Troubleshooting for BN PAGE - (reply: 10)
52. SDS PAGE Destaining - (reply: 7)
53. Layer of water above separating gel mixes into gel - (reply: 2)
54. PCR & Western Blot sample preperation - (reply: 2)
55. SDS page - (reply: 8)
56. Two-night primary antibody incubation? - (reply: 2)
57. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
58. A question about Gelanalyzer - (reply: 8)
59. storing membrane - (reply: 2)
60. band obove the ladder - (reply: 3)

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