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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
32. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
33. Antibody Issues - (reply: 6)
34. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
35. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
36. Is it possible to "over-block" a membrane? - (reply: 5)
37. Troubleshooting for BN PAGE - (reply: 10)
38. SDS PAGE Destaining - (reply: 7)
39. Layer of water above separating gel mixes into gel - (reply: 2)
40. PCR & Western Blot sample preperation - (reply: 2)
41. SDS page - (reply: 8)
42. Two-night primary antibody incubation? - (reply: 2)
43. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
44. A question about Gelanalyzer - (reply: 8)
45. storing membrane - (reply: 2)
46. band obove the ladder - (reply: 3)
47. DNA SDS-PAGE electrophoresis - (reply: 6)
48. Axin1 Co-IP - (reply: 2)
49. Glycine vs. tricine - (reply: 1)
50. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
51. SDS-PAGE power supply issues - (reply: 3)
52. Result in the lab is not the same as published - (reply: 3)
53. PKC theta - (reply: 3)
54. reason for curved bands? - (reply: 2)
55. Protein bands visible on unstained SDS-PAGE - (reply: 1)
56. Inconsistent stripping - (reply: 3)
57. After Co Ip can Iuse SDS PAGE - (reply: 1)
58. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
59. BCIP/NBT developed blot disappearing - (reply: 1)
60. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)

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