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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
31. Heating to denature and reduce protein: same temperature? - (reply: 1)
32. Neurotoxicity of acrylamide - (reply: 2)
33. 2 bands very close in the expected place - (reply: 2)
34. Pressing Too Hard on Transfer Sandwich - (reply: 3)
35. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
36. SDS-PAGE High MW bands dissapear! - (reply: 7)
37. Non-specific binding of protein in western blot - (reply: 3)
38. SDS-PAGE vertical lane streaking - (reply: 1)
39. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
40. Storing membrane after antibody incubation - (reply: 2)
41. layer in between resolving and stacking gel - (reply: 1)
42. Lane spillover (?) on Western Blot - (reply: 3)
43. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
44. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
45. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
46. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
47. No band detected in WB - (reply: 4)
48. Concentration of Sample Buffer to boil protein samples - (reply: 2)
49. Western Blot diffuse bands - (reply: 3)
50. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
51. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
52. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
53. Some bands transferred, some not..? Why??? - (reply: 1)
54. Digital imager questions on correction - (reply: 4)
55. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
56. Rapidly decomposing HRP substrate - (reply: 2)
57. protein size in SDS-PAGE - (reply: 4)
58. Protein not migrating through gel - (reply: 9)
59. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
60. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)

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