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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Problems with protein estimation and western blots - (reply: 1)
422. Loading controls - (reply: 1)
423. Quantity of protein to charge - (reply: 1)
424. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
425. Tris-Hcl becomes cloudy. - (reply: 3)
426. Can FLAG IP, but cannot detect with Western - (reply: 4)
427. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
428. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
429. Native PAGE, interaction of proteins, validity - (reply: 4)
430. Smeared transfer on nitrocellulose membrane - (reply: 2)
431. blocking and primary antibody in different buffers? - (reply: 1)
432. Same non specific band for different target proteins - (reply: 1)
433. Types of 96-well plates used for BCA protein quantification - (reply: 1)
434. Dot blot halo - (reply: 4)
435. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
436. Western blot and isoelectric point - (reply: 1)
437. Western Blot Developing Cassette - (reply: 1)
438. Pull down assay and Co- IP experiment - (reply: 6)
439. Trouble detecting Histaq - (reply: 4)
440. Western gels running super slow! - (reply: 3)
441. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
442. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
443. WB parametric or non-parametric - (reply: 6)
444. Trouble with the GFP signal - (reply: 1)
445. Intern Needing Help - (reply: 3)
446. SDS-Electrophoresis, Dye color change - (reply: 1)
447. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
448. Trouble with equal loading - (reply: 8)
449. how many times striping can b done of the blot - (reply: 9)
450. SDS-PAGE of negatively charged protein - (reply: 4)

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