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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
422. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
423. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
424. restaining of coommassie destained gel - (reply: 1)
425. Secondary antibody overnight? - (reply: 5)
426. Problems with protein estimation and western blots - (reply: 1)
427. Loading controls - (reply: 1)
428. Quantity of protein to charge - (reply: 1)
429. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
430. Tris-Hcl becomes cloudy. - (reply: 3)
431. Can FLAG IP, but cannot detect with Western - (reply: 4)
432. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
433. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
434. Native PAGE, interaction of proteins, validity - (reply: 4)
435. Smeared transfer on nitrocellulose membrane - (reply: 2)
436. blocking and primary antibody in different buffers? - (reply: 1)
437. Same non specific band for different target proteins - (reply: 1)
438. Types of 96-well plates used for BCA protein quantification - (reply: 1)
439. Dot blot halo - (reply: 4)
440. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
441. Western blot and isoelectric point - (reply: 1)
442. Western Blot Developing Cassette - (reply: 1)
443. Pull down assay and Co- IP experiment - (reply: 6)
444. Trouble detecting Histaq - (reply: 4)
445. Western gels running super slow! - (reply: 3)
446. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
447. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
448. WB parametric or non-parametric - (reply: 6)
449. Trouble with the GFP signal - (reply: 1)
450. Intern Needing Help - (reply: 3)

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