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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
422. Antibody Optimization - (reply: 2)
423. Protein agregation in WB samples - (reply: 3)
424. Same Non-specific bands show up for different Abs!! - (reply: 7)
425. what caused bubble band of my WB? - (reply: 4)
426. blotting detection - (reply: 3)
427. Max amount of protein - (reply: 4)
428. Areas of membrane bleached - (reply: 1)
429. asking suggestions for western blot - (reply: 3)
430. Can i apply primary antibody for two nights instead of one? - (reply: 3)
431. Strange bands and large smear in western blot - (reply: 4)
432. Freezing proteins at -80 - (reply: 1)
433. Tris glycine SDS buffer prep pH error - (reply: 2)
434. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
435. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
436. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
437. problems with negative control - (reply: 2)
438. urgent-W.B visualisation on kodak film - (reply: 2)
439. NP 40 lysis buffer - (reply: 1)
440. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
441. Dark western blot film - (reply: 12)
442. how to get red of high secondary signal - (reply: 4)
443. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
444. Detecting phosphoproteins - different results when samples rerun (reply: 1)
445. Is loading buffer ok? - (reply: 1)
446. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
447. Antibody vs Antigen concentration effect - (reply: 3)
448. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
449. SDS PAGE smearing of bands - (reply: 2)
450. Fuzzy bands in western blot - (reply: 2)

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