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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. dot blot - (reply: 4)
422. ECL Prime Streamlining and Troubleshootin - (reply: 1)
423. Samples leaking out of wells in gel - (reply: 2)
424. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
425. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
426. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
427. Strange E.coli growth - (reply: 3)
428. Problem with Immunoprecipitated samples - (reply: 2)
429. problems in transfer - (reply: 3)
430. Phospho-JNK - (reply: 9)
431. Bradford reagent for staining gels? - (reply: 1)
432. developing film manually - (reply: 19)
433. non specific bands - (reply: 7)
434. BSA vs Milk - (reply: 2)
435. Western Blot no target bands, show Non-specific bands - (reply: 5)
436. how critical is the pH of tbst? - (reply: 2)
437. Fc fusion protein smearing on SDS PAGE - (reply: 2)
438. what do you recommend for Semi-Dry unit - (reply: 2)
439. Bands disappear after longer exposure - (reply: 4)
440. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
441. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
442. Antibody Optimization - (reply: 2)
443. Protein agregation in WB samples - (reply: 3)
444. Same Non-specific bands show up for different Abs!! - (reply: 7)
445. what caused bubble band of my WB? - (reply: 4)
446. blotting detection - (reply: 3)
447. Max amount of protein - (reply: 4)
448. Areas of membrane bleached - (reply: 1)
449. asking suggestions for western blot - (reply: 3)
450. Can i apply primary antibody for two nights instead of one? - (reply: 3)

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