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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Reuse of SDS-PAGE electrode buffers - (reply: 2)
422. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
423. Western transfer tank heating up - (reply: 2)
424. Tricine PAG - (reply: 1)
425. Simple Western = a western blot revolution? - (reply: 2)
426. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
427. Best phospho-ERK antibody - (reply: 3)
428. what are the best transfer conditions? - (reply: 4)
429. Cell Envelope Proteins Smeared - (reply: 1)
430. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
431. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
432. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
433. restaining of coommassie destained gel - (reply: 1)
434. Secondary antibody overnight? - (reply: 5)
435. Problems with protein estimation and western blots - (reply: 1)
436. Loading controls - (reply: 1)
437. Quantity of protein to charge - (reply: 1)
438. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
439. Tris-Hcl becomes cloudy. - (reply: 3)
440. Can FLAG IP, but cannot detect with Western - (reply: 4)
441. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
442. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
443. Native PAGE, interaction of proteins, validity - (reply: 4)
444. Smeared transfer on nitrocellulose membrane - (reply: 2)
445. blocking and primary antibody in different buffers? - (reply: 1)
446. Same non specific band for different target proteins - (reply: 1)
447. Types of 96-well plates used for BCA protein quantification - (reply: 1)
448. Dot blot halo - (reply: 4)
449. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
450. Western blot and isoelectric point - (reply: 1)

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