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SDS-PAGE-and-Western-Blotting
421.
Missing patches on immunoblot - please help! -
(reply: 7)
422.
stupid question -
(reply: 8)
423.
GAPDH merging lanes -
(reply: 1)
424.
Band at same position using different primary antibodies -
(reply: 3)
425.
Western for very small protein -
(reply: 3)
426.
sds page - hot hot hot - buffer too hot - gel burnt
(reply: 6)
427.
COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems
(reply: 7)
428.
Having trouble with stripping PVDF membrane during WesterDot blot -
(reply: 1)
429.
bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency
(reply: 1)
430.
Western Blot Secondary Antibody Diluent - basic question
(reply: 3)
431.
PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane
(reply: 1)
432.
Re-use PVDF membran without stripping - may I re-block?
(reply: 5)
433.
What cause "downshift" in SDS-PAGE? -
(reply: 5)
434.
Weak Zymography Signals - Mitigating a weak signal for better bands
(reply: 1)
435.
Blue native PAGE problems -
(reply: 1)
436.
running problems SDS-PAGE gel (zymography) -
(reply: 4)
437.
Western unresolved lanes -
(reply: 2)
438.
Doing WB from scratch for the first time. Need some info. -
(reply: 2)
439.
Western blot bovine collagen typ I problem -
(reply: 3)
440.
no band, extremely clean blot -
(reply: 1)
441.
Acrylamide stock -
(reply: 2)
442.
low signal in WB -
(reply: 3)
443.
standard internal marker for quantification -
(reply: 3)
444.
Antibody too much? - Signal not consistent, and fades quickly
(reply: 5)
445.
IgG - unspecific band
(reply: 3)
446.
red ponceau -
(reply: 3)
447.
Noisy fluorescent gels -
(reply: 1)
448.
p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells
(reply: 9)
449.
SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE
(reply: 3)
450.
resolving and stucking gel solutions -
(reply: 2)
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