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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. SDS-PAGE of negatively charged protein - (reply: 4)
422. Protection Lanes? - (reply: 4)
423. Tie-2 westerns high background black lanes - (reply: 13)
424. BSA concentration for blocking - (reply: 5)
425. Identically loaded western bands loose intensity from left to right - (reply: 3)
426. eIF4G western blot - (reply: 3)
427. smear bands in western blot - (reply: 12)
428. Huge background with HT22 cells phophoERK antibody - (reply: 3)
429. How to quantify the protein amount for serum samples ? - (reply: 1)
430. Huge background with HT22 cells phophoERK antibody - (reply: 1)
431. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
432. horizontal smear? - (reply: 2)
433. BSA versus milk in blocking and antibody incubation - (reply: 5)
434. Western blots are 'semi' quantitative? - (reply: 12)
435. bands fuse together - (reply: 1)
436. Phospho-protein detection - (reply: 2)
437. Help on using different antibodies in the same membrane - (reply: 8)
438. weird query for Western Blotting - (reply: 2)
439. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
440. Quick/One-step/automated Western Blot - (reply: 8)
441. Amount of antibody used in Western Blot - (reply: 1)
442. WB Transfer: Voltage vs. Current - (reply: 7)
443. Large protein blot ~400kDa - (reply: 3)
444. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
445. Stripping buffer for nitrocellulose membrane - (reply: 1)
446. PAGE for Separation of DNA - (reply: 3)
447. Western Blotting of Proteins less than 10 kDa - (reply: 1)
448. dot blot - (reply: 7)
449. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
450. Internal Standard for western blot - (reply: 1)

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