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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
422. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
423. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
424. Reuse of SDS-PAGE electrode buffers - (reply: 2)
425. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
426. Western transfer tank heating up - (reply: 2)
427. Tricine PAG - (reply: 1)
428. Simple Western = a western blot revolution? - (reply: 2)
429. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
430. Best phospho-ERK antibody - (reply: 3)
431. what are the best transfer conditions? - (reply: 4)
432. Cell Envelope Proteins Smeared - (reply: 1)
433. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
434. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
435. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
436. restaining of coommassie destained gel - (reply: 1)
437. Secondary antibody overnight? - (reply: 5)
438. Problems with protein estimation and western blots - (reply: 1)
439. Loading controls - (reply: 1)
440. Quantity of protein to charge - (reply: 1)
441. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
442. Tris-Hcl becomes cloudy. - (reply: 3)
443. Can FLAG IP, but cannot detect with Western - (reply: 4)
444. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
445. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
446. Native PAGE, interaction of proteins, validity - (reply: 4)
447. Smeared transfer on nitrocellulose membrane - (reply: 2)
448. blocking and primary antibody in different buffers? - (reply: 1)
449. Same non specific band for different target proteins - (reply: 1)
450. Types of 96-well plates used for BCA protein quantification - (reply: 1)

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