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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Simple Western = a western blot revolution? - (reply: 2)
422. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
423. Best phospho-ERK antibody - (reply: 3)
424. what are the best transfer conditions? - (reply: 4)
425. Cell Envelope Proteins Smeared - (reply: 1)
426. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
427. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
428. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
429. restaining of coommassie destained gel - (reply: 1)
430. Secondary antibody overnight? - (reply: 5)
431. Problems with protein estimation and western blots - (reply: 1)
432. Loading controls - (reply: 1)
433. Quantity of protein to charge - (reply: 1)
434. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
435. Tris-Hcl becomes cloudy. - (reply: 3)
436. Can FLAG IP, but cannot detect with Western - (reply: 4)
437. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
438. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
439. Native PAGE, interaction of proteins, validity - (reply: 4)
440. Smeared transfer on nitrocellulose membrane - (reply: 2)
441. blocking and primary antibody in different buffers? - (reply: 1)
442. Same non specific band for different target proteins - (reply: 1)
443. Types of 96-well plates used for BCA protein quantification - (reply: 1)
444. Dot blot halo - (reply: 4)
445. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
446. Western blot and isoelectric point - (reply: 1)
447. Western Blot Developing Cassette - (reply: 1)
448. Pull down assay and Co- IP experiment - (reply: 6)
449. Trouble detecting Histaq - (reply: 4)
450. Western gels running super slow! - (reply: 3)

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