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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Best phospho-ERK antibody - (reply: 3)
422. what are the best transfer conditions? - (reply: 4)
423. Cell Envelope Proteins Smeared - (reply: 1)
424. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
425. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
426. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
427. restaining of coommassie destained gel - (reply: 1)
428. Secondary antibody overnight? - (reply: 5)
429. Problems with protein estimation and western blots - (reply: 1)
430. Loading controls - (reply: 1)
431. Quantity of protein to charge - (reply: 1)
432. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
433. Tris-Hcl becomes cloudy. - (reply: 3)
434. Can FLAG IP, but cannot detect with Western - (reply: 4)
435. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
436. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
437. Native PAGE, interaction of proteins, validity - (reply: 4)
438. Smeared transfer on nitrocellulose membrane - (reply: 2)
439. blocking and primary antibody in different buffers? - (reply: 1)
440. Same non specific band for different target proteins - (reply: 1)
441. Types of 96-well plates used for BCA protein quantification - (reply: 1)
442. Dot blot halo - (reply: 4)
443. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
444. Western blot and isoelectric point - (reply: 1)
445. Western Blot Developing Cassette - (reply: 1)
446. Pull down assay and Co- IP experiment - (reply: 6)
447. Trouble detecting Histaq - (reply: 4)
448. Western gels running super slow! - (reply: 3)
449. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
450. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)

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