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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
422. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
423. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
424. Strange E.coli growth - (reply: 3)
425. Problem with Immunoprecipitated samples - (reply: 2)
426. problems in transfer - (reply: 3)
427. Phospho-JNK - (reply: 9)
428. Bradford reagent for staining gels? - (reply: 1)
429. developing film manually - (reply: 19)
430. non specific bands - (reply: 7)
431. BSA vs Milk - (reply: 2)
432. Western Blot no target bands, show Non-specific bands - (reply: 5)
433. how critical is the pH of tbst? - (reply: 2)
434. Fc fusion protein smearing on SDS PAGE - (reply: 2)
435. what do you recommend for Semi-Dry unit - (reply: 2)
436. Bands disappear after longer exposure - (reply: 4)
437. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
438. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
439. Antibody Optimization - (reply: 2)
440. Protein agregation in WB samples - (reply: 3)
441. Same Non-specific bands show up for different Abs!! - (reply: 7)
442. what caused bubble band of my WB? - (reply: 4)
443. blotting detection - (reply: 3)
444. Max amount of protein - (reply: 4)
445. Areas of membrane bleached - (reply: 1)
446. asking suggestions for western blot - (reply: 3)
447. Can i apply primary antibody for two nights instead of one? - (reply: 3)
448. Strange bands and large smear in western blot - (reply: 4)
449. Freezing proteins at -80 - (reply: 1)
450. Tris glycine SDS buffer prep pH error - (reply: 2)

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