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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Can i apply primary antibody for two nights instead of one? - (reply: 3)
422. Strange bands and large smear in western blot - (reply: 4)
423. Freezing proteins at -80 - (reply: 1)
424. Tris glycine SDS buffer prep pH error - (reply: 2)
425. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
426. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
427. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
428. problems with negative control - (reply: 2)
429. urgent-W.B visualisation on kodak film - (reply: 2)
430. NP 40 lysis buffer - (reply: 1)
431. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
432. Dark western blot film - (reply: 12)
433. how to get red of high secondary signal - (reply: 4)
434. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
435. Detecting phosphoproteins - different results when samples rerun (reply: 1)
436. Is loading buffer ok? - (reply: 1)
437. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
438. Antibody vs Antigen concentration effect - (reply: 3)
439. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
440. SDS PAGE smearing of bands - (reply: 2)
441. Fuzzy bands in western blot - (reply: 2)
442. I am desperated with my WB!! - (reply: 2)
443. storing SDS-PAGE gels for W.Blot - (reply: 7)
444. trouble detecting phospho-proteins - (reply: 2)
445. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
446. BSA + DTT versus -DTT - (reply: 1)
447. WB - Transferring REALLY big proteins - (reply: 2)
448. Over-stripping blot? - (reply: 5)
449. Oscillation on my Western Blot results - (reply: 3)
450. HELP!!! Non-specific binding on my membrane - (reply: 7)

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