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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Quantity of protein to charge - (reply: 1)
422. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
423. Tris-Hcl becomes cloudy. - (reply: 3)
424. Can FLAG IP, but cannot detect with Western - (reply: 4)
425. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
426. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
427. Native PAGE, interaction of proteins, validity - (reply: 4)
428. Smeared transfer on nitrocellulose membrane - (reply: 2)
429. blocking and primary antibody in different buffers? - (reply: 1)
430. Same non specific band for different target proteins - (reply: 1)
431. Types of 96-well plates used for BCA protein quantification - (reply: 1)
432. Dot blot halo - (reply: 4)
433. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
434. Western blot and isoelectric point - (reply: 1)
435. Western Blot Developing Cassette - (reply: 1)
436. Pull down assay and Co- IP experiment - (reply: 6)
437. Trouble detecting Histaq - (reply: 4)
438. Western gels running super slow! - (reply: 3)
439. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
440. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
441. WB parametric or non-parametric - (reply: 6)
442. Trouble with the GFP signal - (reply: 1)
443. Intern Needing Help - (reply: 3)
444. SDS-Electrophoresis, Dye color change - (reply: 1)
445. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
446. Trouble with equal loading - (reply: 8)
447. how many times striping can b done of the blot - (reply: 9)
448. SDS-PAGE of negatively charged protein - (reply: 4)
449. Protection Lanes? - (reply: 4)
450. Tie-2 westerns high background black lanes - (reply: 13)

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