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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. lysing mammalian cells in sds - (reply: 3)
422. Western blot mystery - possibly a sample preparation problem? - (reply: 11)
423. How to store a membrane? - (reply: 1)
424. Sample does not sit in the well despite (glycerol checked) - (reply: 4)
425. Western blot sample loading question - (reply: 1)
426. help for the method of meat sample preperation for SDS PAGE 1D - (reply: 4)
427. stripping too much? - (reply: 1)
428. sample precipitation problem in western blotting - (reply: 2)
429. Problem: mouse antibodies with mouse tissues - (reply: 4)
430. which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
431. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
432. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
433. Reuse of SDS-PAGE electrode buffers - (reply: 2)
434. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
435. Western transfer tank heating up - (reply: 2)
436. Tricine PAG - (reply: 1)
437. Simple Western = a western blot revolution? - (reply: 2)
438. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
439. Best phospho-ERK antibody - (reply: 3)
440. what are the best transfer conditions? - (reply: 4)
441. Cell Envelope Proteins Smeared - (reply: 1)
442. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
443. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
444. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
445. restaining of coommassie destained gel - (reply: 1)
446. Secondary antibody overnight? - (reply: 5)
447. Problems with protein estimation and western blots - (reply: 1)
448. Loading controls - (reply: 1)
449. Quantity of protein to charge - (reply: 1)
450. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)

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