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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
421. Missing patches on immunoblot - please help! - (reply: 7)
422. stupid question - (reply: 8)
423. GAPDH merging lanes - (reply: 1)
424. Band at same position using different primary antibodies - (reply: 3)
425. Western for very small protein - (reply: 3)
426. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
427. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
428. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
429. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
430. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
431. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
432. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
433. What cause "downshift" in SDS-PAGE? - (reply: 5)
434. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
435. Blue native PAGE problems - (reply: 1)
436. running problems SDS-PAGE gel (zymography) - (reply: 4)
437. Western unresolved lanes - (reply: 2)
438. Doing WB from scratch for the first time. Need some info. - (reply: 2)
439. Western blot bovine collagen typ I problem - (reply: 3)
440. no band, extremely clean blot - (reply: 1)
441. Acrylamide stock - (reply: 2)
442. low signal in WB - (reply: 3)
443. standard internal marker for quantification - (reply: 3)
444. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
445. IgG - unspecific band (reply: 3)
446. red ponceau - (reply: 3)
447. Noisy fluorescent gels - (reply: 1)
448. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
449. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
450. resolving and stucking gel solutions - (reply: 2)

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