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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. stability of the stripping buffer - (reply: 2)
662. small proteins have run ahead of tracking dye - (reply: 1)
663. cytoplasmic and nuclear extraction - (reply: 2)
664. Equal Protein Quantity - Loading onto Well (reply: 6)
665. Help, odd pattern - pic included - (reply: 3)
666. Fermentas prestained protein marker issue! - (reply: 7)
667. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
668. Invitrogen NuPAGE Sample buffer - (reply: 1)
669. western exposure one part of band darker - (reply: 6)
670. non-specific bands - (reply: 5)
671. Crazy looking BN-PAGE gel - No, really. (reply: 5)
672. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
673. bands of protein seems like it did not move - (reply: 2)
674. downshifted band - (reply: 4)
675. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
676. Western Troubleshooting Help, please! - (reply: 3)
677. Re-use membrane in Western Blot - (reply: 3)
678. NP40 or Triton as membrane protein detergent - (reply: 1)
679. Dot blot- great, western blot - no signal - (reply: 3)
680. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
681. Problem in transfer high MW protein - (reply: 5)
682. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
683. dimerization or aggregation of my protein! - (reply: 3)
684. basic question in SDS and western blot - (reply: 1)
685. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
686. Transfer problems - (reply: 1)
687. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
688. Stripping temperature - have no 55C water bath (reply: 1)
689. Nonspecificity of anti-flag - (reply: 2)
690. Blocking buffer - (reply: 7)

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