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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. problems with His tagged protein western blots - (reply: 3)
662. Springerlink--Zymography - (reply: 3)
663. Methanol vs Isopropanol in fixing solution - (reply: 1)
664. Protein aggregates SDS-PAGE - (reply: 5)
665. probably DNA is disturbing the quantification of my sample - (reply: 3)
666. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
667. Optimising antibody concentration - hints and tips (reply: 1)
668. Lanes all run together - (reply: 5)
669. Protein Markers - (reply: 1)
670. Gel Running Crooked?? Please help.. - (reply: 7)
671. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
672. Strength of actin signal - How dark should the band be? (reply: 5)
673. Immunoprecipitation - (reply: 2)
674. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
675. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
676. SEmi-dry transfer blotting - (reply: 1)
677. Whole cell lysis of fibroblasts - (reply: 1)
678. SDS-PAGE: Strange Running - (reply: 8)
679. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
680. Different TBST pH's for WB incubation, why? - (reply: 1)
681. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
682. stability of the stripping buffer - (reply: 2)
683. small proteins have run ahead of tracking dye - (reply: 1)
684. cytoplasmic and nuclear extraction - (reply: 2)
685. Equal Protein Quantity - Loading onto Well (reply: 6)
686. Help, odd pattern - pic included - (reply: 3)
687. Fermentas prestained protein marker issue! - (reply: 7)
688. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
689. Invitrogen NuPAGE Sample buffer - (reply: 1)
690. western exposure one part of band darker - (reply: 6)

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