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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
662. SDS PAGE- HELP - Bands not visible (reply: 4)
663. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
664. Strange SDS gel after Western Blotting - (reply: 1)
665. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
666. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
667. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
668. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
669. Western blot fail, time and time again - (reply: 21)
670. Western blot of urine samples - Problems verifying loading (reply: 1)
671. BSA test for native PAGE - (reply: 2)
672. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
673. transfer: amperes or volts? - (reply: 6)
674. dot blot works, western doesn't - HELP! - (reply: 5)
675. Liposome + IgG sample in SDS-PAGE - (reply: 1)
676. SDS-PAGE for proteins which have pI>7 - (reply: 1)
677. band diffusion in sds - (reply: 5)
678. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
679. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
680. Artifacts only in Reduced samples!!! - (reply: 18)
681. Over-current alarm - (reply: 1)
682. too much bands in western - (reply: 2)
683. PVDF membrane dried after transfer (Western) - (reply: 6)
684. Help Stained after blocking and won't unstain!!! - (reply: 1)
685. Bands are lower than actual protein - (reply: 1)
686. Protein lysate storage - (reply: 2)
687. Is there alpha-tubulin in the nucleus? - (reply: 4)
688. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
689. No band after WB revelation - (reply: 2)
690. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)

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