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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
662. resolving and stucking gel solutions - (reply: 2)
663. bradford absorbance - (reply: 1)
664. protein degradation - (reply: 3)
665. Dry or not PVDF membrane after transfer - (reply: 2)
666. I see bands with western blot only the third day! - (reply: 2)
667. Western Blots on Membrane Proteins - (reply: 9)
668. Help- voltage going sky high- don't know why - (reply: 1)
669. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
670. efficient stripping buffer - (reply: 2)
671. quantification of a protein in tissue - (reply: 3)
672. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
673. Help - proteins faster than the dye front - (reply: 8)
674. Longer incubation in primary antibody - (reply: 2)
675. Cell Lysis Buffer - (reply: 6)
676. Strange Western blot background - (reply: 2)
677. question about denaturing - (reply: 1)
678. Secondary antibody recovery - (reply: 4)
679. Help! I left my blot in primary at room temp overnight - (reply: 1)
680. Help! I left my blot in primary at room temp overnight - (reply: 1)
681. Help with Twist antibody? - (reply: 1)
682. Denaturing protein before blotting - (reply: 1)
683. Lysis buffer selection - (reply: 2)
684. not completed transfer proteins on NT membrane - western blot (reply: 2)
685. loading buffer and western blot help! - (reply: 5)
686. "Sandy WB" - (reply: 9)
687. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
688. SDS PAGE- HELP - Bands not visible (reply: 4)
689. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
690. Strange SDS gel after Western Blotting - (reply: 1)

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