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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. not completed transfer proteins on NT membrane - western blot (reply: 2)
662. loading buffer and western blot help! - (reply: 5)
663. "Sandy WB" - (reply: 9)
664. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
665. SDS PAGE- HELP - Bands not visible (reply: 4)
666. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
667. Strange SDS gel after Western Blotting - (reply: 1)
668. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
669. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
670. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
671. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
672. Western blot fail, time and time again - (reply: 21)
673. Western blot of urine samples - Problems verifying loading (reply: 1)
674. BSA test for native PAGE - (reply: 2)
675. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
676. transfer: amperes or volts? - (reply: 6)
677. dot blot works, western doesn't - HELP! - (reply: 5)
678. Liposome + IgG sample in SDS-PAGE - (reply: 1)
679. SDS-PAGE for proteins which have pI>7 - (reply: 1)
680. band diffusion in sds - (reply: 5)
681. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
682. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
683. Artifacts only in Reduced samples!!! - (reply: 18)
684. Over-current alarm - (reply: 1)
685. too much bands in western - (reply: 2)
686. PVDF membrane dried after transfer (Western) - (reply: 6)
687. Help Stained after blocking and won't unstain!!! - (reply: 1)
688. Bands are lower than actual protein - (reply: 1)
689. Protein lysate storage - (reply: 2)
690. Is there alpha-tubulin in the nucleus? - (reply: 4)

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