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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
662. Strange SDS gel after Western Blotting - (reply: 1)
663. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
664. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
665. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
666. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
667. Western blot fail, time and time again - (reply: 21)
668. Western blot of urine samples - Problems verifying loading (reply: 1)
669. BSA test for native PAGE - (reply: 2)
670. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
671. transfer: amperes or volts? - (reply: 6)
672. dot blot works, western doesn't - HELP! - (reply: 5)
673. Liposome + IgG sample in SDS-PAGE - (reply: 1)
674. SDS-PAGE for proteins which have pI>7 - (reply: 1)
675. band diffusion in sds - (reply: 5)
676. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
677. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
678. Artifacts only in Reduced samples!!! - (reply: 18)
679. Over-current alarm - (reply: 1)
680. too much bands in western - (reply: 2)
681. PVDF membrane dried after transfer (Western) - (reply: 6)
682. Help Stained after blocking and won't unstain!!! - (reply: 1)
683. Bands are lower than actual protein - (reply: 1)
684. Protein lysate storage - (reply: 2)
685. Is there alpha-tubulin in the nucleus? - (reply: 4)
686. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
687. No band after WB revelation - (reply: 2)
688. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
689. Expression by ICC but not by Western-why? - (reply: 3)
690. Re-Imaging without Stripping - (reply: 4)

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