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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Gel Running Crooked?? Please help.. - (reply: 7)
662. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
663. Strength of actin signal - How dark should the band be? (reply: 5)
664. Immunoprecipitation - (reply: 2)
665. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
666. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
667. SEmi-dry transfer blotting - (reply: 1)
668. Whole cell lysis of fibroblasts - (reply: 1)
669. SDS-PAGE: Strange Running - (reply: 8)
670. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
671. Different TBST pH's for WB incubation, why? - (reply: 1)
672. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
673. stability of the stripping buffer - (reply: 2)
674. small proteins have run ahead of tracking dye - (reply: 1)
675. cytoplasmic and nuclear extraction - (reply: 2)
676. Equal Protein Quantity - Loading onto Well (reply: 6)
677. Help, odd pattern - pic included - (reply: 3)
678. Fermentas prestained protein marker issue! - (reply: 7)
679. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
680. Invitrogen NuPAGE Sample buffer - (reply: 1)
681. western exposure one part of band darker - (reply: 6)
682. non-specific bands - (reply: 5)
683. Crazy looking BN-PAGE gel - No, really. (reply: 5)
684. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
685. bands of protein seems like it did not move - (reply: 2)
686. downshifted band - (reply: 4)
687. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
688. Western Troubleshooting Help, please! - (reply: 3)
689. Re-use membrane in Western Blot - (reply: 3)
690. NP40 or Triton as membrane protein detergent - (reply: 1)

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