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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. I see bands with western blot only the third day! - (reply: 2)
662. Western Blots on Membrane Proteins - (reply: 9)
663. Help- voltage going sky high- don't know why - (reply: 1)
664. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
665. efficient stripping buffer - (reply: 2)
666. quantification of a protein in tissue - (reply: 3)
667. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
668. Help - proteins faster than the dye front - (reply: 8)
669. Longer incubation in primary antibody - (reply: 2)
670. Cell Lysis Buffer - (reply: 6)
671. Strange Western blot background - (reply: 2)
672. question about denaturing - (reply: 1)
673. Secondary antibody recovery - (reply: 4)
674. Help! I left my blot in primary at room temp overnight - (reply: 1)
675. Help! I left my blot in primary at room temp overnight - (reply: 1)
676. Help with Twist antibody? - (reply: 1)
677. Denaturing protein before blotting - (reply: 1)
678. Lysis buffer selection - (reply: 2)
679. not completed transfer proteins on NT membrane - western blot (reply: 2)
680. loading buffer and western blot help! - (reply: 5)
681. "Sandy WB" - (reply: 9)
682. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
683. SDS PAGE- HELP - Bands not visible (reply: 4)
684. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
685. Strange SDS gel after Western Blotting - (reply: 1)
686. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
687. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
688. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
689. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
690. Western blot fail, time and time again - (reply: 21)

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