Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Help with Twist antibody? - (reply: 1)
662. Denaturing protein before blotting - (reply: 1)
663. Lysis buffer selection - (reply: 2)
664. not completed transfer proteins on NT membrane - western blot (reply: 2)
665. loading buffer and western blot help! - (reply: 5)
666. "Sandy WB" - (reply: 9)
667. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
668. SDS PAGE- HELP - Bands not visible (reply: 4)
669. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
670. Strange SDS gel after Western Blotting - (reply: 1)
671. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
672. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
673. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
674. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
675. Western blot fail, time and time again - (reply: 21)
676. Western blot of urine samples - Problems verifying loading (reply: 1)
677. BSA test for native PAGE - (reply: 2)
678. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
679. transfer: amperes or volts? - (reply: 6)
680. dot blot works, western doesn't - HELP! - (reply: 5)
681. Liposome + IgG sample in SDS-PAGE - (reply: 1)
682. SDS-PAGE for proteins which have pI>7 - (reply: 1)
683. band diffusion in sds - (reply: 5)
684. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
685. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
686. Artifacts only in Reduced samples!!! - (reply: 18)
687. Over-current alarm - (reply: 1)
688. too much bands in western - (reply: 2)
689. PVDF membrane dried after transfer (Western) - (reply: 6)
690. Help Stained after blocking and won't unstain!!! - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37