Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
662. No band after WB revelation - (reply: 2)
663. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
664. Expression by ICC but not by Western-why? - (reply: 3)
665. Re-Imaging without Stripping - (reply: 4)
666. MES buffer turned yellow - (reply: 5)
667. western blot woes - (reply: 2)
668. Size larger than expected - totally boggled - (reply: 2)
669. Ponceau Red Staining Problem - (reply: 2)
670. SDS-PAGE Second interface - (reply: 3)
671. biorad's 30% bis/acrylamide storage - (reply: 2)
672. protein concentration per well - (reply: 6)
673. dot blot - Problem (reply: 1)
674. Clear native PAGE - (reply: 1)
675. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
676. Weak western signal for help - (reply: 13)
677. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
678. Western not working anymore - (reply: 15)
679. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
680. BN-PAGE possible with Tris-glycine - (reply: 1)
681. Western few questions - (reply: 1)
682. thiourea in 2D page - What's it for? (reply: 1)
683. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
684. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
685. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
686. Antibody works in immunofluorescence, but not in WB - (reply: 4)
687. B-actin as western blot -control - (reply: 4)
688. horrific western blots , please help - (reply: 3)
689. Trouble with actin, HA and CCND1 :(( - (reply: 1)
690. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35