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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. probably DNA is disturbing the quantification of my sample - (reply: 3)
662. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
663. Optimising antibody concentration - hints and tips (reply: 1)
664. Lanes all run together - (reply: 5)
665. Protein Markers - (reply: 1)
666. Gel Running Crooked?? Please help.. - (reply: 7)
667. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
668. Strength of actin signal - How dark should the band be? (reply: 5)
669. Immunoprecipitation - (reply: 2)
670. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
671. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
672. SEmi-dry transfer blotting - (reply: 1)
673. Whole cell lysis of fibroblasts - (reply: 1)
674. SDS-PAGE: Strange Running - (reply: 8)
675. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
676. Different TBST pH's for WB incubation, why? - (reply: 1)
677. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
678. stability of the stripping buffer - (reply: 2)
679. small proteins have run ahead of tracking dye - (reply: 1)
680. cytoplasmic and nuclear extraction - (reply: 2)
681. Equal Protein Quantity - Loading onto Well (reply: 6)
682. Help, odd pattern - pic included - (reply: 3)
683. Fermentas prestained protein marker issue! - (reply: 7)
684. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
685. Invitrogen NuPAGE Sample buffer - (reply: 1)
686. western exposure one part of band darker - (reply: 6)
687. non-specific bands - (reply: 5)
688. Crazy looking BN-PAGE gel - No, really. (reply: 5)
689. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
690. bands of protein seems like it did not move - (reply: 2)

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