Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Dry or not PVDF membrane after transfer - (reply: 2)
662. I see bands with western blot only the third day! - (reply: 2)
663. Western Blots on Membrane Proteins - (reply: 9)
664. Help- voltage going sky high- don't know why - (reply: 1)
665. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
666. efficient stripping buffer - (reply: 2)
667. quantification of a protein in tissue - (reply: 3)
668. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
669. Help - proteins faster than the dye front - (reply: 8)
670. Longer incubation in primary antibody - (reply: 2)
671. Cell Lysis Buffer - (reply: 6)
672. Strange Western blot background - (reply: 2)
673. question about denaturing - (reply: 1)
674. Secondary antibody recovery - (reply: 4)
675. Help! I left my blot in primary at room temp overnight - (reply: 1)
676. Help! I left my blot in primary at room temp overnight - (reply: 1)
677. Help with Twist antibody? - (reply: 1)
678. Denaturing protein before blotting - (reply: 1)
679. Lysis buffer selection - (reply: 2)
680. not completed transfer proteins on NT membrane - western blot (reply: 2)
681. loading buffer and western blot help! - (reply: 5)
682. "Sandy WB" - (reply: 9)
683. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
684. SDS PAGE- HELP - Bands not visible (reply: 4)
685. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
686. Strange SDS gel after Western Blotting - (reply: 1)
687. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
688. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
689. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
690. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37