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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Help - proteins faster than the dye front - (reply: 8)
662. Longer incubation in primary antibody - (reply: 2)
663. Cell Lysis Buffer - (reply: 6)
664. Strange Western blot background - (reply: 2)
665. question about denaturing - (reply: 1)
666. Secondary antibody recovery - (reply: 4)
667. Help! I left my blot in primary at room temp overnight - (reply: 1)
668. Help! I left my blot in primary at room temp overnight - (reply: 1)
669. Help with Twist antibody? - (reply: 1)
670. Denaturing protein before blotting - (reply: 1)
671. Lysis buffer selection - (reply: 2)
672. not completed transfer proteins on NT membrane - western blot (reply: 2)
673. loading buffer and western blot help! - (reply: 5)
674. "Sandy WB" - (reply: 9)
675. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
676. SDS PAGE- HELP - Bands not visible (reply: 4)
677. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
678. Strange SDS gel after Western Blotting - (reply: 1)
679. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
680. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
681. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
682. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
683. Western blot fail, time and time again - (reply: 21)
684. Western blot of urine samples - Problems verifying loading (reply: 1)
685. BSA test for native PAGE - (reply: 2)
686. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
687. transfer: amperes or volts? - (reply: 6)
688. dot blot works, western doesn't - HELP! - (reply: 5)
689. Liposome + IgG sample in SDS-PAGE - (reply: 1)
690. SDS-PAGE for proteins which have pI>7 - (reply: 1)

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