Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. efficient stripping buffer - (reply: 2)
662. quantification of a protein in tissue - (reply: 3)
663. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
664. Help - proteins faster than the dye front - (reply: 8)
665. Longer incubation in primary antibody - (reply: 2)
666. Cell Lysis Buffer - (reply: 6)
667. Strange Western blot background - (reply: 2)
668. question about denaturing - (reply: 1)
669. Secondary antibody recovery - (reply: 4)
670. Help! I left my blot in primary at room temp overnight - (reply: 1)
671. Help! I left my blot in primary at room temp overnight - (reply: 1)
672. Help with Twist antibody? - (reply: 1)
673. Denaturing protein before blotting - (reply: 1)
674. Lysis buffer selection - (reply: 2)
675. not completed transfer proteins on NT membrane - western blot (reply: 2)
676. loading buffer and western blot help! - (reply: 5)
677. "Sandy WB" - (reply: 9)
678. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
679. SDS PAGE- HELP - Bands not visible (reply: 4)
680. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
681. Strange SDS gel after Western Blotting - (reply: 1)
682. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
683. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
684. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
685. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
686. Western blot fail, time and time again - (reply: 21)
687. Western blot of urine samples - Problems verifying loading (reply: 1)
688. BSA test for native PAGE - (reply: 2)
689. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
690. transfer: amperes or volts? - (reply: 6)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37