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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Denaturing protein before blotting - (reply: 1)
662. Lysis buffer selection - (reply: 2)
663. not completed transfer proteins on NT membrane - western blot (reply: 2)
664. loading buffer and western blot help! - (reply: 5)
665. "Sandy WB" - (reply: 9)
666. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
667. SDS PAGE- HELP - Bands not visible (reply: 4)
668. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
669. Strange SDS gel after Western Blotting - (reply: 1)
670. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
671. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
672. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
673. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
674. Western blot fail, time and time again - (reply: 21)
675. Western blot of urine samples - Problems verifying loading (reply: 1)
676. BSA test for native PAGE - (reply: 2)
677. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
678. transfer: amperes or volts? - (reply: 6)
679. dot blot works, western doesn't - HELP! - (reply: 5)
680. Liposome + IgG sample in SDS-PAGE - (reply: 1)
681. SDS-PAGE for proteins which have pI>7 - (reply: 1)
682. band diffusion in sds - (reply: 5)
683. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
684. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
685. Artifacts only in Reduced samples!!! - (reply: 18)
686. Over-current alarm - (reply: 1)
687. too much bands in western - (reply: 2)
688. PVDF membrane dried after transfer (Western) - (reply: 6)
689. Help Stained after blocking and won't unstain!!! - (reply: 1)
690. Bands are lower than actual protein - (reply: 1)

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