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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Western few questions - (reply: 1)
662. thiourea in 2D page - What's it for? (reply: 1)
663. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
664. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
665. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
666. Antibody works in immunofluorescence, but not in WB - (reply: 4)
667. B-actin as western blot -control - (reply: 4)
668. horrific western blots , please help - (reply: 3)
669. Trouble with actin, HA and CCND1 :(( - (reply: 1)
670. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
671. Lower MW bands than expected. - (reply: 1)
672. Question about reducing agent (DTT) - (reply: 1)
673. Collagen II western blotting - (reply: 1)
674. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
675. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
676. NUCLEAR PROTEIN PREPARATION - (reply: 1)
677. Lost protein from blot? - (reply: 1)
678. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
679. No SDS in gels - (reply: 12)
680. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
681. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
682. Sample loading buffer turning yellow. - (reply: 3)
683. western bands look funny - (reply: 5)
684. IgG SDS-PAGE - (reply: 1)
685. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
686. wet or semi-dry transfer for low MW proteins - (reply: 4)
687. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
688. quantifying "ghost" bands - (reply: 1)
689. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
690. Western blot running and loading samples - (reply: 6)

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