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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Cell Lysis Buffer - (reply: 6)
662. Strange Western blot background - (reply: 2)
663. question about denaturing - (reply: 1)
664. Secondary antibody recovery - (reply: 4)
665. Help! I left my blot in primary at room temp overnight - (reply: 1)
666. Help! I left my blot in primary at room temp overnight - (reply: 1)
667. Help with Twist antibody? - (reply: 1)
668. Denaturing protein before blotting - (reply: 1)
669. Lysis buffer selection - (reply: 2)
670. not completed transfer proteins on NT membrane - western blot (reply: 2)
671. loading buffer and western blot help! - (reply: 5)
672. "Sandy WB" - (reply: 9)
673. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
674. SDS PAGE- HELP - Bands not visible (reply: 4)
675. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
676. Strange SDS gel after Western Blotting - (reply: 1)
677. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
678. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
679. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
680. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
681. Western blot fail, time and time again - (reply: 21)
682. Western blot of urine samples - Problems verifying loading (reply: 1)
683. BSA test for native PAGE - (reply: 2)
684. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
685. transfer: amperes or volts? - (reply: 6)
686. dot blot works, western doesn't - HELP! - (reply: 5)
687. Liposome + IgG sample in SDS-PAGE - (reply: 1)
688. SDS-PAGE for proteins which have pI>7 - (reply: 1)
689. band diffusion in sds - (reply: 5)
690. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)

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