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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
661. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
662. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
663. Sample loading buffer turning yellow. - (reply: 3)
664. western bands look funny - (reply: 5)
665. IgG SDS-PAGE - (reply: 1)
666. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
667. wet or semi-dry transfer for low MW proteins - (reply: 4)
668. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
669. quantifying "ghost" bands - (reply: 1)
670. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
671. Western blot running and loading samples - (reply: 6)
672. problems with His tagged protein western blots - (reply: 3)
673. Springerlink--Zymography - (reply: 3)
674. Methanol vs Isopropanol in fixing solution - (reply: 1)
675. Protein aggregates SDS-PAGE - (reply: 5)
676. probably DNA is disturbing the quantification of my sample - (reply: 3)
677. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
678. Optimising antibody concentration - hints and tips (reply: 1)
679. Lanes all run together - (reply: 5)
680. Protein Markers - (reply: 1)
681. Gel Running Crooked?? Please help.. - (reply: 7)
682. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
683. Strength of actin signal - How dark should the band be? (reply: 5)
684. Immunoprecipitation - (reply: 2)
685. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
686. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
687. SEmi-dry transfer blotting - (reply: 1)
688. Whole cell lysis of fibroblasts - (reply: 1)
689. SDS-PAGE: Strange Running - (reply: 8)
690. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)

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