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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Ghost and inverse bands and high background. Help! - (reply: 3)
212. No resolving of protein/bands below 50kDa :-s - (reply: 5)
213. Problems with wet transfer blot - (reply: 2)
214. 5% BSA blocking buffer - (reply: 3)
215. Storage of Membrane before addition of primary antibody - (reply: 3)
216. "Mickey Mouse" bands - (reply: 5)
217. SDS-PAGE : when the transfer stops - (reply: 1)
218. confusing Western results - (reply: 5)
219. aditional band on WB - (reply: 1)
220. Transferring >150 kDA proteins conditions - (reply: 3)
221. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
222. TAU gel electrophoresis of histones - (reply: 4)
223. Bands dark on sides and light in middle - (reply: 7)
224. Addition of sample loading buffer, before or after heating - (reply: 5)
225. question about protein expression normalization. - (reply: 6)
226. Protein bands not migrating - (reply: 3)
227. Help with high background (have tried most trouble shooting) - (reply: 8)
228. Tumor protein precipitation at denaturing step? - (reply: 1)
229. How to prepare blood serum for western blot? - (reply: 3)
230. TNF alpha and IL 1b Western Blot - (reply: 1)
231. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
232. SDS-PAGE trouble with protein concentration - (reply: 2)
233. Acrylamid - (reply: 5)
234. Detecting doublet bands in Western for eGFP control - (reply: 1)
235. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
236. Protein Size 50 kDa higher than expected - (reply: 3)
237. Acid urea gel electrophoresis (AUGE) - (reply: 7)
238. Phospho-protein vs total protein Western blot - (reply: 5)
239. storing the loaded gel before running it - (reply: 7)
240. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)

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