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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Premixing gel solutions - (reply: 2)
212. getting negative protein measurements - (reply: 3)
213. Peculiar background problem with MPM-2 antibodies - (reply: 2)
214. Stripping the membrane for LiCor - (reply: 1)
215. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
216. Lysis buffer - (reply: 2)
217. SDS Page and WB: Wrong size bands - (reply: 2)
218. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
219. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
220. How to avoid gel breakage after electrophoresis - (reply: 5)
221. What is an Input in SDS-PAGE? - (reply: 1)
222. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
223. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
224. Good loading control for native gels? - (reply: 1)
225. Please help with odd bands and smears on Western blot - (reply: 4)
226. How to load equal amount of protein without using spectramax - (reply: 4)
227. Western blot membrane imaging - (reply: 7)
228. Detection of T7 RNA polymerase by WB - (reply: 6)
229. Shift in the position of band onto the western blot - (reply: 3)
230. Ghost and inverse bands and high background. Help! - (reply: 3)
231. No resolving of protein/bands below 50kDa :-s - (reply: 5)
232. Problems with wet transfer blot - (reply: 2)
233. 5% BSA blocking buffer - (reply: 3)
234. Storage of Membrane before addition of primary antibody - (reply: 3)
235. "Mickey Mouse" bands - (reply: 5)
236. SDS-PAGE : when the transfer stops - (reply: 1)
237. confusing Western results - (reply: 5)
238. aditional band on WB - (reply: 1)
239. Transferring >150 kDA proteins conditions - (reply: 3)
240. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)

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