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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
212. Problem with GFP detection in western - (reply: 6)
213. Sudden Trouble with PVDF Transfer - (reply: 6)
214. Equal Loading Confusion - (reply: 6)
215. Wrong protein size on western blot - (reply: 10)
216. How to know if the signal of western blot is saturated ? - (reply: 4)
217. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
218. 5x SDS lysis buffer - (reply: 5)
219. Protein measurement of Laemmli samples - (reply: 2)
220. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
221. Uneven banding - Western blot troubleshooting - (reply: 4)
222. Western Blot with fixed cells - (reply: 5)
223. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
224. Primary antibody - (reply: 1)
225. Native-PAGE, help! - (reply: 3)
226. Trouble with purified recombinant protein bands - (reply: 3)
227. Stain in electrophoresis is going from + to - - (reply: 3)
228. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
229. Extraction of Transmembrane Proteins - (reply: 1)
230. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
231. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
232. Protein amount to load for publication figure? - (reply: 4)
233. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
234. What is different between Native protein and Denatured protein samples. - (reply: 4)
235. 3 extra bands show up on my western after an IP - (reply: 2)
236. IgG heavy chain from IP - (reply: 8)
237. about western blot analysis - (reply: 1)
238. Western Blotting loading control for large proteins - (reply: 2)
239. Uneven beta actin - (reply: 2)
240. Minimum volume antibody solution for Western blot in tray? - (reply: 4)

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