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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. about western blot analysis - (reply: 1)
212. Western Blotting loading control for large proteins - (reply: 2)
213. Uneven beta actin - (reply: 2)
214. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
215. NCOR1 western - (reply: 8)
216. Discrepancy between WB and Flow cytometry result - (reply: 5)
217. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
218. Western blotting transfer - (reply: 2)
219. Western Quantification (No BCA???) - (reply: 2)
220. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
221. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
222. Trouble shooting in Far-western blot. - (reply: 1)
223. Transcription/Translation reaction not resolving - (reply: 7)
224. Ponceau red problem: membrane turned red - (reply: 3)
225. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
226. No bands/weird bands on western blot - (reply: 3)
227. Protein ladder not separating - (reply: 6)
228. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
229. Preparing supernatant from cell culture for western blot - (reply: 4)
230. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
231. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
232. Curved bands in Western Blot - (reply: 2)
233. Some bands not coming up, others uneven - (reply: 9)
234. Western blot and Triton X-100 in blocking buffer - (reply: 1)
235. Dot blot translation to WB - (reply: 2)
236. Reference protein give different expression - (reply: 3)
237. Excess betamercaptoethanol during sample preparation - (reply: 4)
238. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
239. Far western blot: probe concentration - (reply: 1)
240. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)

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