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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
212. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
213. Protein amount to load for publication figure? - (reply: 4)
214. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
215. What is different between Native protein and Denatured protein samples. - (reply: 4)
216. 3 extra bands show up on my western after an IP - (reply: 2)
217. IgG heavy chain from IP - (reply: 8)
218. about western blot analysis - (reply: 1)
219. Western Blotting loading control for large proteins - (reply: 2)
220. Uneven beta actin - (reply: 2)
221. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
222. NCOR1 western - (reply: 8)
223. Discrepancy between WB and Flow cytometry result - (reply: 5)
224. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
225. Western blotting transfer - (reply: 2)
226. Western Quantification (No BCA???) - (reply: 2)
227. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
228. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
229. Trouble shooting in Far-western blot. - (reply: 1)
230. Transcription/Translation reaction not resolving - (reply: 7)
231. Ponceau red problem: membrane turned red - (reply: 3)
232. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
233. No bands/weird bands on western blot - (reply: 3)
234. Protein ladder not separating - (reply: 6)
235. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
236. Preparing supernatant from cell culture for western blot - (reply: 4)
237. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
238. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
239. Curved bands in Western Blot - (reply: 2)
240. Some bands not coming up, others uneven - (reply: 9)

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