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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Protein amount to load for publication figure? - (reply: 4)
212. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
213. What is different between Native protein and Denatured protein samples. - (reply: 4)
214. 3 extra bands show up on my western after an IP - (reply: 2)
215. IgG heavy chain from IP - (reply: 8)
216. about western blot analysis - (reply: 1)
217. Western Blotting loading control for large proteins - (reply: 2)
218. Uneven beta actin - (reply: 2)
219. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
220. NCOR1 western - (reply: 8)
221. Discrepancy between WB and Flow cytometry result - (reply: 5)
222. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
223. Western blotting transfer - (reply: 2)
224. Western Quantification (No BCA???) - (reply: 2)
225. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
226. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
227. Trouble shooting in Far-western blot. - (reply: 1)
228. Transcription/Translation reaction not resolving - (reply: 7)
229. Ponceau red problem: membrane turned red - (reply: 3)
230. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
231. No bands/weird bands on western blot - (reply: 3)
232. Protein ladder not separating - (reply: 6)
233. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
234. Preparing supernatant from cell culture for western blot - (reply: 4)
235. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
236. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
237. Curved bands in Western Blot - (reply: 2)
238. Some bands not coming up, others uneven - (reply: 9)
239. Western blot and Triton X-100 in blocking buffer - (reply: 1)
240. Dot blot translation to WB - (reply: 2)

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