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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Transferring >150 kDA proteins conditions - (reply: 3)
212. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
213. TAU gel electrophoresis of histones - (reply: 4)
214. Bands dark on sides and light in middle - (reply: 7)
215. Addition of sample loading buffer, before or after heating - (reply: 5)
216. question about protein expression normalization. - (reply: 6)
217. Protein bands not migrating - (reply: 3)
218. Help with high background (have tried most trouble shooting) - (reply: 8)
219. Tumor protein precipitation at denaturing step? - (reply: 1)
220. How to prepare blood serum for western blot? - (reply: 3)
221. TNF alpha and IL 1b Western Blot - (reply: 1)
222. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
223. SDS-PAGE trouble with protein concentration - (reply: 2)
224. Acrylamid - (reply: 5)
225. Detecting doublet bands in Western for eGFP control - (reply: 1)
226. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
227. Protein Size 50 kDa higher than expected - (reply: 3)
228. Acid urea gel electrophoresis (AUGE) - (reply: 7)
229. Phospho-protein vs total protein Western blot - (reply: 5)
230. storing the loaded gel before running it - (reply: 7)
231. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
232. Western blot trouble shooting - (reply: 3)
233. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
234. two bands on WB-alternative use of start codons? - (reply: 2)
235. TCA Precipitation to remove albumin - (reply: 6)
236. adjusting pH of 4x stacking buffer - (reply: 3)
237. No WB bands for H2AX at all...! - (reply: 10)
238. any tips for doing Fluorescence Western Blot? - (reply: 2)
239. Unknown silver/grey residue left after transfer. - (reply: 2)
240. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)

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