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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. No bands/weird bands on western blot - (reply: 3)
212. Protein ladder not separating - (reply: 6)
213. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
214. Preparing supernatant from cell culture for western blot - (reply: 4)
215. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
216. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
217. Curved bands in Western Blot - (reply: 2)
218. Some bands not coming up, others uneven - (reply: 9)
219. Western blot and Triton X-100 in blocking buffer - (reply: 1)
220. Dot blot translation to WB - (reply: 2)
221. Reference protein give different expression - (reply: 3)
222. Excess betamercaptoethanol during sample preparation - (reply: 4)
223. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
224. Far western blot: probe concentration - (reply: 1)
225. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
226. Low Protein Content In Cell Lysate - (reply: 1)
227. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
228. B- actin band looks like dumbbells! - (reply: 2)
229. western blot explenation - (reply: 8)
230. WB of cellular proteins in human serum - (reply: 2)
231. Western blotting - (reply: 9)
232. How long can I store cell lysis buffer at 4C for? - (reply: 1)
233. Which antibody is good for HA-tag with low background bands? - (reply: 1)
234. Blocking solution mistake, please help! - (reply: 2)
235. Membrane Protein SDS PAGE and WB - (reply: 1)
236. Wet transfer of proteins between 100-200kDa - (reply: 2)
237. western blot and actin issue - (reply: 2)
238. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)
239. conjugated antibodies in immunoprecipitation - (reply: 3)
240. Separating proteing with difference of 5Kd on western blot - (reply: 4)

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