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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Extraction of Transmembrane Proteins - (reply: 1)
212. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
213. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
214. Protein amount to load for publication figure? - (reply: 4)
215. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
216. What is different between Native protein and Denatured protein samples. - (reply: 4)
217. 3 extra bands show up on my western after an IP - (reply: 2)
218. IgG heavy chain from IP - (reply: 8)
219. about western blot analysis - (reply: 1)
220. Western Blotting loading control for large proteins - (reply: 2)
221. Uneven beta actin - (reply: 2)
222. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
223. NCOR1 western - (reply: 8)
224. Discrepancy between WB and Flow cytometry result - (reply: 5)
225. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
226. Western blotting transfer - (reply: 2)
227. Western Quantification (No BCA???) - (reply: 2)
228. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
229. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
230. Trouble shooting in Far-western blot. - (reply: 1)
231. Transcription/Translation reaction not resolving - (reply: 7)
232. Ponceau red problem: membrane turned red - (reply: 3)
233. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
234. No bands/weird bands on western blot - (reply: 3)
235. Protein ladder not separating - (reply: 6)
236. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
237. Preparing supernatant from cell culture for western blot - (reply: 4)
238. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
239. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
240. Curved bands in Western Blot - (reply: 2)

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