Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Stain in electrophoresis is going from + to - - (reply: 3)
212. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
213. Extraction of Transmembrane Proteins - (reply: 1)
214. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
215. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
216. Protein amount to load for publication figure? - (reply: 4)
217. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
218. What is different between Native protein and Denatured protein samples. - (reply: 4)
219. 3 extra bands show up on my western after an IP - (reply: 2)
220. IgG heavy chain from IP - (reply: 8)
221. about western blot analysis - (reply: 1)
222. Western Blotting loading control for large proteins - (reply: 2)
223. Uneven beta actin - (reply: 2)
224. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
225. NCOR1 western - (reply: 8)
226. Discrepancy between WB and Flow cytometry result - (reply: 5)
227. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
228. Western blotting transfer - (reply: 2)
229. Western Quantification (No BCA???) - (reply: 2)
230. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
231. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
232. Trouble shooting in Far-western blot. - (reply: 1)
233. Transcription/Translation reaction not resolving - (reply: 7)
234. Ponceau red problem: membrane turned red - (reply: 3)
235. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
236. No bands/weird bands on western blot - (reply: 3)
237. Protein ladder not separating - (reply: 6)
238. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
239. Preparing supernatant from cell culture for western blot - (reply: 4)
240. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37