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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
212. Primary antibody - (reply: 1)
213. Native-PAGE, help! - (reply: 3)
214. Trouble with purified recombinant protein bands - (reply: 3)
215. Stain in electrophoresis is going from + to - - (reply: 3)
216. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
217. Extraction of Transmembrane Proteins - (reply: 1)
218. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
219. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
220. Protein amount to load for publication figure? - (reply: 4)
221. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
222. What is different between Native protein and Denatured protein samples. - (reply: 4)
223. 3 extra bands show up on my western after an IP - (reply: 2)
224. IgG heavy chain from IP - (reply: 8)
225. about western blot analysis - (reply: 1)
226. Western Blotting loading control for large proteins - (reply: 2)
227. Uneven beta actin - (reply: 2)
228. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
229. NCOR1 western - (reply: 8)
230. Discrepancy between WB and Flow cytometry result - (reply: 5)
231. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
232. Western blotting transfer - (reply: 2)
233. Western Quantification (No BCA???) - (reply: 2)
234. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
235. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
236. Trouble shooting in Far-western blot. - (reply: 1)
237. Transcription/Translation reaction not resolving - (reply: 7)
238. Ponceau red problem: membrane turned red - (reply: 3)
239. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
240. No bands/weird bands on western blot - (reply: 3)

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