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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Western blot membrane imaging - (reply: 7)
212. Detection of T7 RNA polymerase by WB - (reply: 6)
213. Shift in the position of band onto the western blot - (reply: 3)
214. Ghost and inverse bands and high background. Help! - (reply: 3)
215. No resolving of protein/bands below 50kDa :-s - (reply: 5)
216. Problems with wet transfer blot - (reply: 2)
217. 5% BSA blocking buffer - (reply: 3)
218. Storage of Membrane before addition of primary antibody - (reply: 3)
219. "Mickey Mouse" bands - (reply: 5)
220. SDS-PAGE : when the transfer stops - (reply: 1)
221. confusing Western results - (reply: 5)
222. aditional band on WB - (reply: 1)
223. Transferring >150 kDA proteins conditions - (reply: 3)
224. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
225. TAU gel electrophoresis of histones - (reply: 4)
226. Bands dark on sides and light in middle - (reply: 7)
227. Addition of sample loading buffer, before or after heating - (reply: 5)
228. question about protein expression normalization. - (reply: 6)
229. Protein bands not migrating - (reply: 3)
230. Help with high background (have tried most trouble shooting) - (reply: 8)
231. Tumor protein precipitation at denaturing step? - (reply: 1)
232. How to prepare blood serum for western blot? - (reply: 3)
233. TNF alpha and IL 1b Western Blot - (reply: 1)
234. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
235. SDS-PAGE trouble with protein concentration - (reply: 2)
236. Acrylamid - (reply: 5)
237. Detecting doublet bands in Western for eGFP control - (reply: 1)
238. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
239. Protein Size 50 kDa higher than expected - (reply: 3)
240. Acid urea gel electrophoresis (AUGE) - (reply: 7)

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