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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. Western Blot with fixed cells - (reply: 5)
212. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
213. Primary antibody - (reply: 1)
214. Native-PAGE, help! - (reply: 3)
215. Trouble with purified recombinant protein bands - (reply: 3)
216. Stain in electrophoresis is going from + to - - (reply: 3)
217. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
218. Extraction of Transmembrane Proteins - (reply: 1)
219. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
220. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
221. Protein amount to load for publication figure? - (reply: 4)
222. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
223. What is different between Native protein and Denatured protein samples. - (reply: 4)
224. 3 extra bands show up on my western after an IP - (reply: 2)
225. IgG heavy chain from IP - (reply: 8)
226. about western blot analysis - (reply: 1)
227. Western Blotting loading control for large proteins - (reply: 2)
228. Uneven beta actin - (reply: 2)
229. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
230. NCOR1 western - (reply: 8)
231. Discrepancy between WB and Flow cytometry result - (reply: 5)
232. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
233. Western blotting transfer - (reply: 2)
234. Western Quantification (No BCA???) - (reply: 2)
235. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
236. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
237. Trouble shooting in Far-western blot. - (reply: 1)
238. Transcription/Translation reaction not resolving - (reply: 7)
239. Ponceau red problem: membrane turned red - (reply: 3)
240. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)

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