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211. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
212. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
213. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
214. restaining of coommassie destained gel - (reply: 1)
215. Secondary antibody overnight? - (reply: 5)
216. Problems with protein estimation and western blots - (reply: 1)
217. Loading controls - (reply: 1)
218. Quantity of protein to charge - (reply: 1)
219. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
220. Tris-Hcl becomes cloudy. - (reply: 3)
221. Can FLAG IP, but cannot detect with Western - (reply: 4)
222. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
223. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
224. Native PAGE, interaction of proteins, validity - (reply: 4)
225. Smeared transfer on nitrocellulose membrane - (reply: 2)
226. blocking and primary antibody in different buffers? - (reply: 1)
227. Same non specific band for different target proteins - (reply: 1)
228. Types of 96-well plates used for BCA protein quantification - (reply: 1)
229. Dot blot halo - (reply: 4)
230. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
231. Western blot and isoelectric point - (reply: 1)
232. Western Blot Developing Cassette - (reply: 1)
233. Pull down assay and Co- IP experiment - (reply: 6)
234. Trouble detecting Histaq - (reply: 4)
235. Western gels running super slow! - (reply: 3)
236. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
237. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
238. WB parametric or non-parametric - (reply: 6)
239. Trouble with the GFP signal - (reply: 1)
240. Intern Needing Help - (reply: 3)
212. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
213. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
214. restaining of coommassie destained gel - (reply: 1)
215. Secondary antibody overnight? - (reply: 5)
216. Problems with protein estimation and western blots - (reply: 1)
217. Loading controls - (reply: 1)
218. Quantity of protein to charge - (reply: 1)
219. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
220. Tris-Hcl becomes cloudy. - (reply: 3)
221. Can FLAG IP, but cannot detect with Western - (reply: 4)
222. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
223. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
224. Native PAGE, interaction of proteins, validity - (reply: 4)
225. Smeared transfer on nitrocellulose membrane - (reply: 2)
226. blocking and primary antibody in different buffers? - (reply: 1)
227. Same non specific band for different target proteins - (reply: 1)
228. Types of 96-well plates used for BCA protein quantification - (reply: 1)
229. Dot blot halo - (reply: 4)
230. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
231. Western blot and isoelectric point - (reply: 1)
232. Western Blot Developing Cassette - (reply: 1)
233. Pull down assay and Co- IP experiment - (reply: 6)
234. Trouble detecting Histaq - (reply: 4)
235. Western gels running super slow! - (reply: 3)
236. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
237. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
238. WB parametric or non-parametric - (reply: 6)
239. Trouble with the GFP signal - (reply: 1)
240. Intern Needing Help - (reply: 3)