Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
211. 3 extra bands show up on my western after an IP - (reply: 2)
212. IgG heavy chain from IP - (reply: 8)
213. about western blot analysis - (reply: 1)
214. Western Blotting loading control for large proteins - (reply: 2)
215. Uneven beta actin - (reply: 2)
216. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
217. NCOR1 western - (reply: 8)
218. Discrepancy between WB and Flow cytometry result - (reply: 5)
219. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
220. Western blotting transfer - (reply: 2)
221. Western Quantification (No BCA???) - (reply: 2)
222. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
223. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
224. Trouble shooting in Far-western blot. - (reply: 1)
225. Transcription/Translation reaction not resolving - (reply: 7)
226. Ponceau red problem: membrane turned red - (reply: 3)
227. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
228. No bands/weird bands on western blot - (reply: 3)
229. Protein ladder not separating - (reply: 6)
230. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
231. Preparing supernatant from cell culture for western blot - (reply: 4)
232. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
233. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
234. Curved bands in Western Blot - (reply: 2)
235. Some bands not coming up, others uneven - (reply: 9)
236. Western blot and Triton X-100 in blocking buffer - (reply: 1)
237. Dot blot translation to WB - (reply: 2)
238. Reference protein give different expression - (reply: 3)
239. Excess betamercaptoethanol during sample preparation - (reply: 4)
240. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36