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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. blotting detection - (reply: 3)
542. Max amount of protein - (reply: 4)
543. Areas of membrane bleached - (reply: 1)
544. asking suggestions for western blot - (reply: 3)
545. Can i apply primary antibody for two nights instead of one? - (reply: 3)
546. Strange bands and large smear in western blot - (reply: 4)
547. Freezing proteins at -80 - (reply: 1)
548. Tris glycine SDS buffer prep pH error - (reply: 2)
549. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
550. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
551. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
552. problems with negative control - (reply: 2)
553. urgent-W.B visualisation on kodak film - (reply: 2)
554. NP 40 lysis buffer - (reply: 1)
555. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
556. Dark western blot film - (reply: 12)
557. how to get red of high secondary signal - (reply: 4)
558. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
559. Detecting phosphoproteins - different results when samples rerun (reply: 1)
560. Is loading buffer ok? - (reply: 1)
561. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
562. Antibody vs Antigen concentration effect - (reply: 3)
563. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
564. SDS PAGE smearing of bands - (reply: 2)
565. Fuzzy bands in western blot - (reply: 2)
566. I am desperated with my WB!! - (reply: 2)
567. storing SDS-PAGE gels for W.Blot - (reply: 7)
568. trouble detecting phospho-proteins - (reply: 2)
569. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
570. BSA + DTT versus -DTT - (reply: 1)

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