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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
542. What cause "downshift" in SDS-PAGE? - (reply: 5)
543. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
544. Blue native PAGE problems - (reply: 1)
545. running problems SDS-PAGE gel (zymography) - (reply: 4)
546. Western unresolved lanes - (reply: 2)
547. Doing WB from scratch for the first time. Need some info. - (reply: 2)
548. Western blot bovine collagen typ I problem - (reply: 3)
549. no band, extremely clean blot - (reply: 1)
550. Acrylamide stock - (reply: 2)
551. low signal in WB - (reply: 3)
552. standard internal marker for quantification - (reply: 3)
553. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
554. IgG - unspecific band (reply: 3)
555. red ponceau - (reply: 3)
556. Noisy fluorescent gels - (reply: 1)
557. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
558. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
559. resolving and stucking gel solutions - (reply: 2)
560. bradford absorbance - (reply: 1)
561. protein degradation - (reply: 3)
562. Dry or not PVDF membrane after transfer - (reply: 2)
563. I see bands with western blot only the third day! - (reply: 2)
564. Western Blots on Membrane Proteins - (reply: 9)
565. Help- voltage going sky high- don't know why - (reply: 1)
566. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
567. efficient stripping buffer - (reply: 2)
568. quantification of a protein in tissue - (reply: 3)
569. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
570. Help - proteins faster than the dye front - (reply: 8)

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