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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Acrylamide stock - (reply: 2)
542. low signal in WB - (reply: 3)
543. standard internal marker for quantification - (reply: 3)
544. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
545. IgG - unspecific band (reply: 3)
546. red ponceau - (reply: 3)
547. Noisy fluorescent gels - (reply: 1)
548. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
549. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
550. resolving and stucking gel solutions - (reply: 2)
551. bradford absorbance - (reply: 1)
552. protein degradation - (reply: 3)
553. Dry or not PVDF membrane after transfer - (reply: 2)
554. I see bands with western blot only the third day! - (reply: 2)
555. Western Blots on Membrane Proteins - (reply: 9)
556. Help- voltage going sky high- don't know why - (reply: 1)
557. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
558. efficient stripping buffer - (reply: 2)
559. quantification of a protein in tissue - (reply: 3)
560. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
561. Help - proteins faster than the dye front - (reply: 8)
562. Longer incubation in primary antibody - (reply: 2)
563. Cell Lysis Buffer - (reply: 6)
564. Strange Western blot background - (reply: 2)
565. question about denaturing - (reply: 1)
566. Secondary antibody recovery - (reply: 4)
567. Help! I left my blot in primary at room temp overnight - (reply: 1)
568. Help! I left my blot in primary at room temp overnight - (reply: 1)
569. Help with Twist antibody? - (reply: 1)
570. Denaturing protein before blotting - (reply: 1)

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