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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Bands disappear after longer exposure - (reply: 4)
542. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
543. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
544. Antibody Optimization - (reply: 2)
545. Protein agregation in WB samples - (reply: 3)
546. Same Non-specific bands show up for different Abs!! - (reply: 7)
547. what caused bubble band of my WB? - (reply: 4)
548. blotting detection - (reply: 3)
549. Max amount of protein - (reply: 4)
550. Areas of membrane bleached - (reply: 1)
551. asking suggestions for western blot - (reply: 3)
552. Can i apply primary antibody for two nights instead of one? - (reply: 3)
553. Strange bands and large smear in western blot - (reply: 4)
554. Freezing proteins at -80 - (reply: 1)
555. Tris glycine SDS buffer prep pH error - (reply: 2)
556. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
557. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
558. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
559. problems with negative control - (reply: 2)
560. urgent-W.B visualisation on kodak film - (reply: 2)
561. NP 40 lysis buffer - (reply: 1)
562. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
563. Dark western blot film - (reply: 12)
564. how to get red of high secondary signal - (reply: 4)
565. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
566. Detecting phosphoproteins - different results when samples rerun (reply: 1)
567. Is loading buffer ok? - (reply: 1)
568. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
569. Antibody vs Antigen concentration effect - (reply: 3)
570. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)

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