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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Can i apply primary antibody for two nights instead of one? - (reply: 3)
542. Strange bands and large smear in western blot - (reply: 4)
543. Freezing proteins at -80 - (reply: 1)
544. Tris glycine SDS buffer prep pH error - (reply: 2)
545. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
546. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
547. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
548. problems with negative control - (reply: 2)
549. urgent-W.B visualisation on kodak film - (reply: 2)
550. NP 40 lysis buffer - (reply: 1)
551. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
552. Dark western blot film - (reply: 12)
553. how to get red of high secondary signal - (reply: 4)
554. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
555. Detecting phosphoproteins - different results when samples rerun (reply: 1)
556. Is loading buffer ok? - (reply: 1)
557. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
558. Antibody vs Antigen concentration effect - (reply: 3)
559. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
560. SDS PAGE smearing of bands - (reply: 2)
561. Fuzzy bands in western blot - (reply: 2)
562. I am desperated with my WB!! - (reply: 2)
563. storing SDS-PAGE gels for W.Blot - (reply: 7)
564. trouble detecting phospho-proteins - (reply: 2)
565. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
566. BSA + DTT versus -DTT - (reply: 1)
567. WB - Transferring REALLY big proteins - (reply: 2)
568. Over-stripping blot? - (reply: 5)
569. Oscillation on my Western Blot results - (reply: 3)
570. HELP!!! Non-specific binding on my membrane - (reply: 7)

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