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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Antibody Optimization - (reply: 2)
542. Protein agregation in WB samples - (reply: 3)
543. Same Non-specific bands show up for different Abs!! - (reply: 7)
544. what caused bubble band of my WB? - (reply: 4)
545. blotting detection - (reply: 3)
546. Max amount of protein - (reply: 4)
547. Areas of membrane bleached - (reply: 1)
548. asking suggestions for western blot - (reply: 3)
549. Can i apply primary antibody for two nights instead of one? - (reply: 3)
550. Strange bands and large smear in western blot - (reply: 4)
551. Freezing proteins at -80 - (reply: 1)
552. Tris glycine SDS buffer prep pH error - (reply: 2)
553. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
554. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
555. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
556. problems with negative control - (reply: 2)
557. urgent-W.B visualisation on kodak film - (reply: 2)
558. NP 40 lysis buffer - (reply: 1)
559. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
560. Dark western blot film - (reply: 12)
561. how to get red of high secondary signal - (reply: 4)
562. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
563. Detecting phosphoproteins - different results when samples rerun (reply: 1)
564. Is loading buffer ok? - (reply: 1)
565. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
566. Antibody vs Antigen concentration effect - (reply: 3)
567. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
568. SDS PAGE smearing of bands - (reply: 2)
569. Fuzzy bands in western blot - (reply: 2)
570. I am desperated with my WB!! - (reply: 2)

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