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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Is loading buffer ok? - (reply: 1)
542. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
543. Antibody vs Antigen concentration effect - (reply: 3)
544. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
545. SDS PAGE smearing of bands - (reply: 2)
546. Fuzzy bands in western blot - (reply: 2)
547. I am desperated with my WB!! - (reply: 2)
548. storing SDS-PAGE gels for W.Blot - (reply: 7)
549. trouble detecting phospho-proteins - (reply: 2)
550. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
551. BSA + DTT versus -DTT - (reply: 1)
552. WB - Transferring REALLY big proteins - (reply: 2)
553. Over-stripping blot? - (reply: 5)
554. Oscillation on my Western Blot results - (reply: 3)
555. HELP!!! Non-specific binding on my membrane - (reply: 7)
556. Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)
557. Many unspecific bands after using anti-His on Western blot - (reply: 4)
558. Need help with pre run of PAGE. - (reply: 1)
559. KO mice show band for knocked-out protein - (reply: 1)
560. SDS-PAGE gel wont separate small proteins - (reply: 5)
561. SDS Acrylamide Gel Equipement - Need to purchase best and cheapest new equipment (reply: 3)
562. can't get bands in Western Blot - (reply: 5)
563. Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
564. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
565. Converging lanes in Western - (reply: 8)
566. Incomplete transfer - (reply: 7)
567. Anyone has run a PAGE with reducing agents in-gel?? - (reply: 3)
568. Non reducing protein ladder? - (reply: 5)
569. When to wash and how many times? - (reply: 4)
570. SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)

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