|
541. Protein Markers - (reply: 1)
542. Gel Running Crooked?? Please help.. - (reply: 7)
543. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
544. Strength of actin signal - How dark should the band be? (reply: 5)
545. Immunoprecipitation - (reply: 2)
546. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
547. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
548. SEmi-dry transfer blotting - (reply: 1)
549. Whole cell lysis of fibroblasts - (reply: 1)
550. SDS-PAGE: Strange Running - (reply: 8)
551. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
552. Different TBST pH's for WB incubation, why? - (reply: 1)
553. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
554. stability of the stripping buffer - (reply: 2)
555. small proteins have run ahead of tracking dye - (reply: 1)
556. cytoplasmic and nuclear extraction - (reply: 2)
557. Equal Protein Quantity - Loading onto Well (reply: 6)
558. Help, odd pattern - pic included - (reply: 3)
559. Fermentas prestained protein marker issue! - (reply: 7)
560. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
561. Invitrogen NuPAGE Sample buffer - (reply: 1)
562. western exposure one part of band darker - (reply: 6)
563. non-specific bands - (reply: 5)
564. Crazy looking BN-PAGE gel - No, really. (reply: 5)
565. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
566. bands of protein seems like it did not move - (reply: 2)
567. downshifted band - (reply: 4)
568. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
569. Western Troubleshooting Help, please! - (reply: 3)
570. Re-use membrane in Western Blot - (reply: 3)
542. Gel Running Crooked?? Please help.. - (reply: 7)
543. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
544. Strength of actin signal - How dark should the band be? (reply: 5)
545. Immunoprecipitation - (reply: 2)
546. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
547. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
548. SEmi-dry transfer blotting - (reply: 1)
549. Whole cell lysis of fibroblasts - (reply: 1)
550. SDS-PAGE: Strange Running - (reply: 8)
551. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
552. Different TBST pH's for WB incubation, why? - (reply: 1)
553. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
554. stability of the stripping buffer - (reply: 2)
555. small proteins have run ahead of tracking dye - (reply: 1)
556. cytoplasmic and nuclear extraction - (reply: 2)
557. Equal Protein Quantity - Loading onto Well (reply: 6)
558. Help, odd pattern - pic included - (reply: 3)
559. Fermentas prestained protein marker issue! - (reply: 7)
560. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
561. Invitrogen NuPAGE Sample buffer - (reply: 1)
562. western exposure one part of band darker - (reply: 6)
563. non-specific bands - (reply: 5)
564. Crazy looking BN-PAGE gel - No, really. (reply: 5)
565. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
566. bands of protein seems like it did not move - (reply: 2)
567. downshifted band - (reply: 4)
568. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
569. Western Troubleshooting Help, please! - (reply: 3)
570. Re-use membrane in Western Blot - (reply: 3)