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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Freezing proteins at -80 - (reply: 1)
542. Tris glycine SDS buffer prep pH error - (reply: 2)
543. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
544. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
545. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
546. problems with negative control - (reply: 2)
547. urgent-W.B visualisation on kodak film - (reply: 2)
548. NP 40 lysis buffer - (reply: 1)
549. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
550. Dark western blot film - (reply: 12)
551. how to get red of high secondary signal - (reply: 4)
552. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
553. Detecting phosphoproteins - different results when samples rerun (reply: 1)
554. Is loading buffer ok? - (reply: 1)
555. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
556. Antibody vs Antigen concentration effect - (reply: 3)
557. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
558. SDS PAGE smearing of bands - (reply: 2)
559. Fuzzy bands in western blot - (reply: 2)
560. I am desperated with my WB!! - (reply: 2)
561. storing SDS-PAGE gels for W.Blot - (reply: 7)
562. trouble detecting phospho-proteins - (reply: 2)
563. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
564. BSA + DTT versus -DTT - (reply: 1)
565. WB - Transferring REALLY big proteins - (reply: 2)
566. Over-stripping blot? - (reply: 5)
567. Oscillation on my Western Blot results - (reply: 3)
568. HELP!!! Non-specific binding on my membrane - (reply: 7)
569. Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)
570. Many unspecific bands after using anti-His on Western blot - (reply: 4)

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