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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Doing WB from scratch for the first time. Need some info. - (reply: 2)
542. Western blot bovine collagen typ I problem - (reply: 3)
543. no band, extremely clean blot - (reply: 1)
544. Acrylamide stock - (reply: 2)
545. low signal in WB - (reply: 3)
546. standard internal marker for quantification - (reply: 3)
547. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
548. IgG - unspecific band (reply: 3)
549. red ponceau - (reply: 3)
550. Noisy fluorescent gels - (reply: 1)
551. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
552. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
553. resolving and stucking gel solutions - (reply: 2)
554. bradford absorbance - (reply: 1)
555. protein degradation - (reply: 3)
556. Dry or not PVDF membrane after transfer - (reply: 2)
557. I see bands with western blot only the third day! - (reply: 2)
558. Western Blots on Membrane Proteins - (reply: 9)
559. Help- voltage going sky high- don't know why - (reply: 1)
560. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
561. efficient stripping buffer - (reply: 2)
562. quantification of a protein in tissue - (reply: 3)
563. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
564. Help - proteins faster than the dye front - (reply: 8)
565. Longer incubation in primary antibody - (reply: 2)
566. Cell Lysis Buffer - (reply: 6)
567. Strange Western blot background - (reply: 2)
568. question about denaturing - (reply: 1)
569. Secondary antibody recovery - (reply: 4)
570. Help! I left my blot in primary at room temp overnight - (reply: 1)

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