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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
541. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
542. Antibody Optimization - (reply: 2)
543. Protein agregation in WB samples - (reply: 3)
544. Same Non-specific bands show up for different Abs!! - (reply: 7)
545. what caused bubble band of my WB? - (reply: 4)
546. blotting detection - (reply: 3)
547. Max amount of protein - (reply: 4)
548. Areas of membrane bleached - (reply: 1)
549. asking suggestions for western blot - (reply: 3)
550. Can i apply primary antibody for two nights instead of one? - (reply: 3)
551. Strange bands and large smear in western blot - (reply: 4)
552. Freezing proteins at -80 - (reply: 1)
553. Tris glycine SDS buffer prep pH error - (reply: 2)
554. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
555. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
556. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
557. problems with negative control - (reply: 2)
558. urgent-W.B visualisation on kodak film - (reply: 2)
559. NP 40 lysis buffer - (reply: 1)
560. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
561. Dark western blot film - (reply: 12)
562. how to get red of high secondary signal - (reply: 4)
563. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
564. Detecting phosphoproteins - different results when samples rerun (reply: 1)
565. Is loading buffer ok? - (reply: 1)
566. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
567. Antibody vs Antigen concentration effect - (reply: 3)
568. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
569. SDS PAGE smearing of bands - (reply: 2)
570. Fuzzy bands in western blot - (reply: 2)

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