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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
391. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
392. restaining of coommassie destained gel - (reply: 1)
393. Secondary antibody overnight? - (reply: 5)
394. Problems with protein estimation and western blots - (reply: 1)
395. Loading controls - (reply: 1)
396. Quantity of protein to charge - (reply: 1)
397. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
398. Tris-Hcl becomes cloudy. - (reply: 3)
399. Can FLAG IP, but cannot detect with Western - (reply: 4)
400. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
401. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
402. Native PAGE, interaction of proteins, validity - (reply: 4)
403. Smeared transfer on nitrocellulose membrane - (reply: 2)
404. blocking and primary antibody in different buffers? - (reply: 1)
405. Same non specific band for different target proteins - (reply: 1)
406. Types of 96-well plates used for BCA protein quantification - (reply: 1)
407. Dot blot halo - (reply: 4)
408. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
409. Western blot and isoelectric point - (reply: 1)
410. Western Blot Developing Cassette - (reply: 1)
411. Pull down assay and Co- IP experiment - (reply: 6)
412. Trouble detecting Histaq - (reply: 4)
413. Western gels running super slow! - (reply: 3)
414. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
415. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
416. WB parametric or non-parametric - (reply: 6)
417. Trouble with the GFP signal - (reply: 1)
418. Intern Needing Help - (reply: 3)
419. SDS-Electrophoresis, Dye color change - (reply: 1)
420. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)

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