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SDS-PAGE-and-Western-Blotting
391.
resolving gel pourcentage -
(reply: 3)
392.
dye protein ladder -
(reply: 5)
393.
BAD blots - so many problems they donīt fit in a title
(reply: 3)
394.
Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane)
(reply: 3)
395.
Trouble-shooting Tris-Tricine SDS-PAGE - fussy dye front
(reply: 4)
396.
How do you identify protein detected on western blot from a lysate???? -
(reply: 2)
397.
RIPA Buffer turns Bradford into Blue -
(reply: 2)
398.
freezing antibody lyophilised -
(reply: 2)
399.
Nitrocellulose membrane storage after transfer -
(reply: 6)
400.
western without tween -
(reply: 3)
401.
Bubbles on the marker after transfer -
(reply: 2)
402.
Missing patches on immunoblot - please help! -
(reply: 7)
403.
stupid question -
(reply: 8)
404.
GAPDH merging lanes -
(reply: 1)
405.
Band at same position using different primary antibodies -
(reply: 3)
406.
Western for very small protein -
(reply: 3)
407.
sds page - hot hot hot - buffer too hot - gel burnt
(reply: 6)
408.
COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems
(reply: 7)
409.
Having trouble with stripping PVDF membrane during WesterDot blot -
(reply: 1)
410.
bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency
(reply: 1)
411.
Western Blot Secondary Antibody Diluent - basic question
(reply: 3)
412.
PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane
(reply: 1)
413.
Re-use PVDF membran without stripping - may I re-block?
(reply: 5)
414.
What cause "downshift" in SDS-PAGE? -
(reply: 5)
415.
Weak Zymography Signals - Mitigating a weak signal for better bands
(reply: 1)
416.
Blue native PAGE problems -
(reply: 1)
417.
running problems SDS-PAGE gel (zymography) -
(reply: 4)
418.
Western unresolved lanes -
(reply: 2)
419.
Doing WB from scratch for the first time. Need some info. -
(reply: 2)
420.
Western blot bovine collagen typ I problem -
(reply: 3)
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