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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
391. Pull down assay and Co- IP experiment - (reply: 6)
392. Trouble detecting Histaq - (reply: 4)
393. Western gels running super slow! - (reply: 3)
394. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
395. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
396. WB parametric or non-parametric - (reply: 6)
397. Trouble with the GFP signal - (reply: 1)
398. Intern Needing Help - (reply: 3)
399. SDS-Electrophoresis, Dye color change - (reply: 1)
400. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
401. Trouble with equal loading - (reply: 8)
402. how many times striping can b done of the blot - (reply: 9)
403. SDS-PAGE of negatively charged protein - (reply: 4)
404. Protection Lanes? - (reply: 4)
405. Tie-2 westerns high background black lanes - (reply: 13)
406. BSA concentration for blocking - (reply: 5)
407. Identically loaded western bands loose intensity from left to right - (reply: 3)
408. eIF4G western blot - (reply: 3)
409. smear bands in western blot - (reply: 12)
410. Huge background with HT22 cells phophoERK antibody - (reply: 3)
411. How to quantify the protein amount for serum samples ? - (reply: 1)
412. Huge background with HT22 cells phophoERK antibody - (reply: 1)
413. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
414. horizontal smear? - (reply: 2)
415. BSA versus milk in blocking and antibody incubation - (reply: 5)
416. Western blots are 'semi' quantitative? - (reply: 12)
417. bands fuse together - (reply: 1)
418. Phospho-protein detection - (reply: 2)
419. Help on using different antibodies in the same membrane - (reply: 8)
420. weird query for Western Blotting - (reply: 2)

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