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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
391. Dot blot halo - (reply: 4)
392. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
393. Western blot and isoelectric point - (reply: 1)
394. Western Blot Developing Cassette - (reply: 1)
395. Pull down assay and Co- IP experiment - (reply: 6)
396. Trouble detecting Histaq - (reply: 4)
397. Western gels running super slow! - (reply: 3)
398. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
399. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
400. WB parametric or non-parametric - (reply: 6)
401. Trouble with the GFP signal - (reply: 1)
402. Intern Needing Help - (reply: 3)
403. SDS-Electrophoresis, Dye color change - (reply: 1)
404. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
405. Trouble with equal loading - (reply: 8)
406. how many times striping can b done of the blot - (reply: 9)
407. SDS-PAGE of negatively charged protein - (reply: 4)
408. Protection Lanes? - (reply: 4)
409. Tie-2 westerns high background black lanes - (reply: 13)
410. BSA concentration for blocking - (reply: 5)
411. Identically loaded western bands loose intensity from left to right - (reply: 3)
412. eIF4G western blot - (reply: 3)
413. smear bands in western blot - (reply: 12)
414. Huge background with HT22 cells phophoERK antibody - (reply: 3)
415. How to quantify the protein amount for serum samples ? - (reply: 1)
416. Huge background with HT22 cells phophoERK antibody - (reply: 1)
417. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
418. horizontal smear? - (reply: 2)
419. BSA versus milk in blocking and antibody incubation - (reply: 5)
420. Western blots are 'semi' quantitative? - (reply: 12)

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