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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
391. Use of wrong TBS concentration - (reply: 1)
392. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
393. SDS-PAGE staining for digestion assay - (reply: 1)
394. Can I improve my primary antibody? - (reply: 2)
395. got band in control positive but not in sample... - (reply: 2)
396. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
397. Problems with IPs - (reply: 2)
398. Band is at the wrong size, but same for all antibodies - (reply: 6)
399. Problems with western blot detection - (reply: 1)
400. Methods of concentrating total protein extracts - (reply: 2)
401. Using serum as the sample in Western blot - (reply: 3)
402. Puzzeling western blot results, missing band - (reply: 3)
403. Secondary AB blocking - (reply: 1)
404. western blood immunodetection Ab - (reply: 1)
405. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
406. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
407. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
408. What percentage should I use for a stacking gel? - (reply: 1)
409. DMSO - (reply: 1)
410. several questions on running SDS-PAGE - (reply: 1)
411. Voltage issues during transfer - (reply: 5)
412. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
413. What percentage gel should I make? - (reply: 1)
414. Western Blot problems-vertical streaking - (reply: 3)
415. Protein extraction from dead cells - (reply: 1)
416. Signal stuck on top of seperating gel - (reply: 1)
417. SDS-PAGE gel and running buffer - (reply: 3)
418. The same strong bands with different primary antibody - (reply: 1)
419. Trouble Western Blotting a 360kDa Protein - (reply: 5)
420. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)

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