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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
152. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
153. Low signal, weird background stains on blot - (reply: 5)
154. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
155. Incomplete Blotting and staining of NC membrane - (reply: 7)
156. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
157. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
158. Help, protein ladder didn`t separate - (reply: 3)
159. Determining phosphorylation status of a protein by transfection - (reply: 5)
160. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
161. Problem with Denative lysis - (reply: 1)
162. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
163. Polyclonal antibody precipitating - (reply: 1)
164. manual 2D electrophoresis - (reply: 8)
165. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
166. sample sonication before loading - (reply: 6)
167. transfer of low molecular weight protein - (reply: 3)
168. How is the mass detected via SDS-PAGE? - (reply: 3)
169. Water in beads during boiling - (reply: 3)
170. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
171. Runing problems - (reply: 18)
172. Western Blotting of Amyloid Beta - (reply: 1)
173. 10x Laemmli buffer - (reply: 5)
174. Need help translating my western blot protocol into every language - (reply: 3)
175. Semi-dry system for NuPage with MES buffer - (reply: 4)
176. Recommend anti-rat HRP secondary antibody - (reply: 2)
177. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
178. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
179. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
180. Western blot to CD44? - (reply: 3)

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