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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Native PAGE problem - not entering gel enough - (reply: 9)
152. film saturation in immunoblots - (reply: 1)
153. Problems with wells on SDS PAGE gels - (reply: 3)
154. Protein is present, but not detected by antibody - (reply: 4)
155. secondary antibody creation - (reply: 1)
156. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
157. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
158. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
159. Low signal, weird background stains on blot - (reply: 5)
160. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
161. Incomplete Blotting and staining of NC membrane - (reply: 7)
162. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
163. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
164. Help, protein ladder didn`t separate - (reply: 3)
165. Determining phosphorylation status of a protein by transfection - (reply: 5)
166. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
167. Problem with Denative lysis - (reply: 1)
168. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
169. Polyclonal antibody precipitating - (reply: 1)
170. manual 2D electrophoresis - (reply: 8)
171. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
172. sample sonication before loading - (reply: 6)
173. transfer of low molecular weight protein - (reply: 3)
174. How is the mass detected via SDS-PAGE? - (reply: 3)
175. Water in beads during boiling - (reply: 3)
176. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
177. Runing problems - (reply: 18)
178. Western Blotting of Amyloid Beta - (reply: 1)
179. 10x Laemmli buffer - (reply: 5)
180. Need help translating my western blot protocol into every language - (reply: 3)

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