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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. 10x Laemmli buffer - (reply: 5)
152. Need help translating my western blot protocol into every language - (reply: 3)
153. Semi-dry system for NuPage with MES buffer - (reply: 4)
154. Recommend anti-rat HRP secondary antibody - (reply: 2)
155. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
156. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
157. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
158. Western blot to CD44? - (reply: 3)
159. Amount of EDTA - (reply: 4)
160. SDS Apparatus for northern - (reply: 1)
161. Prey protein band bleeds, IgG band normal? - (reply: 3)
162. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
163. His-Probe - (reply: 2)
164. Problems with western membrane stripping - (reply: 2)
165. Can protein isomers be detected in SDS-PAGE - (reply: 3)
166. western blot of cardiomyocytes - (reply: 2)
167. Gradient Gel Electrophoresis - (reply: 3)
168. SDS-PAGE resolving gel not completely set - (reply: 4)
169. Problem with SDS-PAGE gel - (reply: 13)
170. How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
171. SDS-Page trouble-shooting - (reply: 3)
172. PVDF membrane long-term storage after transfer - (reply: 3)
173. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
174. Distorted bands from human colon samples - (reply: 2)
175. "Wavy" bands in SDS-PAGE gel - (reply: 5)
176. LMW proteins not transfer of gel to membrane - (reply: 1)
177. smear in SDS PAGE - (reply: 4)
178. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
179. Smeared Bands after Ponceau Staining? - (reply: 3)
180. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)

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