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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
152. Low signal, weird background stains on blot - (reply: 5)
153. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
154. Incomplete Blotting and staining of NC membrane - (reply: 7)
155. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
156. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
157. Help, protein ladder didn`t separate - (reply: 3)
158. Determining phosphorylation status of a protein by transfection - (reply: 5)
159. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
160. Problem with Denative lysis - (reply: 1)
161. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
162. Polyclonal antibody precipitating - (reply: 1)
163. manual 2D electrophoresis - (reply: 8)
164. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
165. sample sonication before loading - (reply: 6)
166. transfer of low molecular weight protein - (reply: 3)
167. How is the mass detected via SDS-PAGE? - (reply: 3)
168. Water in beads during boiling - (reply: 3)
169. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
170. Runing problems - (reply: 18)
171. Western Blotting of Amyloid Beta - (reply: 1)
172. 10x Laemmli buffer - (reply: 5)
173. Need help translating my western blot protocol into every language - (reply: 3)
174. Semi-dry system for NuPage with MES buffer - (reply: 4)
175. Recommend anti-rat HRP secondary antibody - (reply: 2)
176. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
177. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
178. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
179. Western blot to CD44? - (reply: 3)
180. Amount of EDTA - (reply: 4)

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