Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Native-PAGE, help! - (reply: 3)
152. Trouble with purified recombinant protein bands - (reply: 3)
153. Stain in electrophoresis is going from + to - - (reply: 3)
154. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
155. Extraction of Transmembrane Proteins - (reply: 1)
156. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
157. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
158. Protein amount to load for publication figure? - (reply: 4)
159. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
160. What is different between Native protein and Denatured protein samples. - (reply: 4)
161. 3 extra bands show up on my western after an IP - (reply: 2)
162. IgG heavy chain from IP - (reply: 8)
163. about western blot analysis - (reply: 1)
164. Western Blotting loading control for large proteins - (reply: 2)
165. Uneven beta actin - (reply: 2)
166. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
167. NCOR1 western - (reply: 8)
168. Discrepancy between WB and Flow cytometry result - (reply: 5)
169. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
170. Western blotting transfer - (reply: 2)
171. Western Quantification (No BCA???) - (reply: 2)
172. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
173. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
174. Trouble shooting in Far-western blot. - (reply: 1)
175. Transcription/Translation reaction not resolving - (reply: 7)
176. Ponceau red problem: membrane turned red - (reply: 3)
177. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
178. No bands/weird bands on western blot - (reply: 3)
179. Protein ladder not separating - (reply: 6)
180. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35