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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
152. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
153. Help, protein ladder didn`t separate - (reply: 3)
154. Determining phosphorylation status of a protein by transfection - (reply: 5)
155. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
156. Problem with Denative lysis - (reply: 1)
157. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
158. Polyclonal antibody precipitating - (reply: 1)
159. manual 2D electrophoresis - (reply: 8)
160. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
161. sample sonication before loading - (reply: 6)
162. transfer of low molecular weight protein - (reply: 3)
163. How is the mass detected via SDS-PAGE? - (reply: 3)
164. Water in beads during boiling - (reply: 3)
165. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
166. Runing problems - (reply: 18)
167. Western Blotting of Amyloid Beta - (reply: 1)
168. 10x Laemmli buffer - (reply: 5)
169. Need help translating my western blot protocol into every language - (reply: 3)
170. Semi-dry system for NuPage with MES buffer - (reply: 4)
171. Recommend anti-rat HRP secondary antibody - (reply: 2)
172. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
173. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
174. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
175. Western blot to CD44? - (reply: 3)
176. Amount of EDTA - (reply: 4)
177. SDS Apparatus for northern - (reply: 1)
178. Prey protein band bleeds, IgG band normal? - (reply: 3)
179. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
180. His-Probe - (reply: 2)

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