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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Peculiar background problem with MPM-2 antibodies - (reply: 2)
152. Stripping the membrane for LiCor - (reply: 1)
153. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
154. Lysis buffer - (reply: 2)
155. SDS Page and WB: Wrong size bands - (reply: 2)
156. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
157. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
158. How to avoid gel breakage after electrophoresis - (reply: 5)
159. What is an Input in SDS-PAGE? - (reply: 1)
160. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
161. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
162. Good loading control for native gels? - (reply: 1)
163. Please help with odd bands and smears on Western blot - (reply: 4)
164. How to load equal amount of protein without using spectramax - (reply: 4)
165. Western blot membrane imaging - (reply: 7)
166. Detection of T7 RNA polymerase by WB - (reply: 6)
167. Shift in the position of band onto the western blot - (reply: 3)
168. Ghost and inverse bands and high background. Help! - (reply: 3)
169. No resolving of protein/bands below 50kDa :-s - (reply: 5)
170. Problems with wet transfer blot - (reply: 2)
171. 5% BSA blocking buffer - (reply: 3)
172. Storage of Membrane before addition of primary antibody - (reply: 3)
173. "Mickey Mouse" bands - (reply: 5)
174. SDS-PAGE : when the transfer stops - (reply: 1)
175. confusing Western results - (reply: 5)
176. aditional band on WB - (reply: 1)
177. Transferring >150 kDA proteins conditions - (reply: 3)
178. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
179. TAU gel electrophoresis of histones - (reply: 4)
180. Bands dark on sides and light in middle - (reply: 7)

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