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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
152. No bands/weird bands on western blot - (reply: 3)
153. Protein ladder not separating - (reply: 6)
154. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
155. Preparing supernatant from cell culture for western blot - (reply: 4)
156. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
157. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
158. Curved bands in Western Blot - (reply: 2)
159. Some bands not coming up, others uneven - (reply: 9)
160. Western blot and Triton X-100 in blocking buffer - (reply: 1)
161. Dot blot translation to WB - (reply: 2)
162. Reference protein give different expression - (reply: 3)
163. Excess betamercaptoethanol during sample preparation - (reply: 4)
164. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
165. Far western blot: probe concentration - (reply: 1)
166. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
167. Low Protein Content In Cell Lysate - (reply: 1)
168. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
169. B- actin band looks like dumbbells! - (reply: 2)
170. western blot explenation - (reply: 8)
171. WB of cellular proteins in human serum - (reply: 2)
172. Western blotting - (reply: 9)
173. How long can I store cell lysis buffer at 4C for? - (reply: 1)
174. Which antibody is good for HA-tag with low background bands? - (reply: 1)
175. Blocking solution mistake, please help! - (reply: 2)
176. Membrane Protein SDS PAGE and WB - (reply: 1)
177. Wet transfer of proteins between 100-200kDa - (reply: 2)
178. western blot and actin issue - (reply: 2)
179. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)
180. conjugated antibodies in immunoprecipitation - (reply: 3)

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