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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
152. Native PAGE problem - not entering gel enough - (reply: 9)
153. film saturation in immunoblots - (reply: 1)
154. Problems with wells on SDS PAGE gels - (reply: 3)
155. Protein is present, but not detected by antibody - (reply: 4)
156. secondary antibody creation - (reply: 1)
157. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
158. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
159. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
160. Low signal, weird background stains on blot - (reply: 5)
161. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
162. Incomplete Blotting and staining of NC membrane - (reply: 7)
163. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
164. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
165. Help, protein ladder didn`t separate - (reply: 3)
166. Determining phosphorylation status of a protein by transfection - (reply: 5)
167. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
168. Problem with Denative lysis - (reply: 1)
169. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
170. Polyclonal antibody precipitating - (reply: 1)
171. manual 2D electrophoresis - (reply: 8)
172. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
173. sample sonication before loading - (reply: 6)
174. transfer of low molecular weight protein - (reply: 3)
175. How is the mass detected via SDS-PAGE? - (reply: 3)
176. Water in beads during boiling - (reply: 3)
177. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
178. Runing problems - (reply: 18)
179. Western Blotting of Amyloid Beta - (reply: 1)
180. 10x Laemmli buffer - (reply: 6)

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