Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Problems with wells on SDS PAGE gels - (reply: 3)
152. Protein is present, but not detected by antibody - (reply: 4)
153. secondary antibody creation - (reply: 1)
154. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
155. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
156. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
157. Low signal, weird background stains on blot - (reply: 5)
158. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
159. Incomplete Blotting and staining of NC membrane - (reply: 7)
160. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
161. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
162. Help, protein ladder didn`t separate - (reply: 3)
163. Determining phosphorylation status of a protein by transfection - (reply: 5)
164. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
165. Problem with Denative lysis - (reply: 1)
166. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
167. Polyclonal antibody precipitating - (reply: 1)
168. manual 2D electrophoresis - (reply: 8)
169. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
170. sample sonication before loading - (reply: 6)
171. transfer of low molecular weight protein - (reply: 3)
172. How is the mass detected via SDS-PAGE? - (reply: 3)
173. Water in beads during boiling - (reply: 3)
174. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
175. Runing problems - (reply: 18)
176. Western Blotting of Amyloid Beta - (reply: 1)
177. 10x Laemmli buffer - (reply: 5)
178. Need help translating my western blot protocol into every language - (reply: 3)
179. Semi-dry system for NuPage with MES buffer - (reply: 4)
180. Recommend anti-rat HRP secondary antibody - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37