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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Help, protein ladder didn`t separate - (reply: 3)
152. Determining phosphorylation status of a protein by transfection - (reply: 5)
153. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
154. Problem with Denative lysis - (reply: 1)
155. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
156. Polyclonal antibody precipitating - (reply: 1)
157. manual 2D electrophoresis - (reply: 8)
158. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
159. sample sonication before loading - (reply: 6)
160. transfer of low molecular weight protein - (reply: 3)
161. How is the mass detected via SDS-PAGE? - (reply: 3)
162. Water in beads during boiling - (reply: 3)
163. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
164. Runing problems - (reply: 18)
165. Western Blotting of Amyloid Beta - (reply: 1)
166. 10x Laemmli buffer - (reply: 5)
167. Need help translating my western blot protocol into every language - (reply: 3)
168. Semi-dry system for NuPage with MES buffer - (reply: 4)
169. Recommend anti-rat HRP secondary antibody - (reply: 2)
170. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
171. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
172. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
173. Western blot to CD44? - (reply: 3)
174. Amount of EDTA - (reply: 4)
175. SDS Apparatus for northern - (reply: 1)
176. Prey protein band bleeds, IgG band normal? - (reply: 3)
177. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
178. His-Probe - (reply: 2)
179. Problems with western membrane stripping - (reply: 2)
180. Can protein isomers be detected in SDS-PAGE - (reply: 3)

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