Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
151. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
152. Preparing supernatant from cell culture for western blot - (reply: 4)
153. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
154. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
155. Curved bands in Western Blot - (reply: 2)
156. Some bands not coming up, others uneven - (reply: 9)
157. Western blot and Triton X-100 in blocking buffer - (reply: 1)
158. Dot blot translation to WB - (reply: 2)
159. Reference protein give different expression - (reply: 3)
160. Excess betamercaptoethanol during sample preparation - (reply: 4)
161. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
162. Far western blot: probe concentration - (reply: 1)
163. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
164. Low Protein Content In Cell Lysate - (reply: 1)
165. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
166. B- actin band looks like dumbbells! - (reply: 2)
167. western blot explenation - (reply: 8)
168. WB of cellular proteins in human serum - (reply: 2)
169. Western blotting - (reply: 9)
170. How long can I store cell lysis buffer at 4C for? - (reply: 1)
171. Which antibody is good for HA-tag with low background bands? - (reply: 1)
172. Blocking solution mistake, please help! - (reply: 2)
173. Membrane Protein SDS PAGE and WB - (reply: 1)
174. Wet transfer of proteins between 100-200kDa - (reply: 2)
175. western blot and actin issue - (reply: 2)
176. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)
177. conjugated antibodies in immunoprecipitation - (reply: 3)
178. Separating proteing with difference of 5Kd on western blot - (reply: 4)
179. small proteins - (reply: 2)
180. SDS PAGE gel storing - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34