Amount of EDTA - (Sep/18/2013 )
I made a silly big mistake by adding twice more the amount of EDTA for the MES buffer...
Will it has much affect on it?
I do not have MES and other chemicals to add to make the amount equivalent for the extra EDTA :(
If the EDTA is a normal concentrations such as 1 mM, then it will little effect.
Suppose i use 1mM of EDTA for 500 mL of 20x MES buffer.
But i use 1 mM of EDTA for 250 mL of 20x MES buffer.
Will it affects? :(
Thanks alot :D