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Amount of EDTA - (Sep/18/2013 )

Dear all, 

I made a silly big mistake by adding twice more the amount of EDTA for the MES buffer...

Will it has much affect on it?

I do not have MES and other chemicals to add to make the amount equivalent for the extra EDTA :(



If the EDTA is a normal concentrations such as 1 mM, then it will little effect.



Suppose i use 1mM of EDTA for 500 mL of 20x MES buffer.

But i use 1 mM of EDTA for 250 mL of 20x MES buffer.

Will it affects? :(





Thanks alot :D