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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. NP40 or Triton as membrane protein detergent - (reply: 1)
722. Dot blot- great, western blot - no signal - (reply: 3)
723. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
724. Problem in transfer high MW protein - (reply: 5)
725. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
726. dimerization or aggregation of my protein! - (reply: 3)
727. basic question in SDS and western blot - (reply: 1)
728. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
729. Transfer problems - (reply: 1)
730. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
731. Stripping temperature - have no 55C water bath (reply: 1)
732. Nonspecificity of anti-flag - (reply: 2)
733. Blocking buffer - (reply: 7)
734. Detection of phospho-proteins - (reply: 1)
735. light chain - (reply: 5)
736. ugly merged bands in western blot - (reply: 3)
737. Western Blot Data Analysis - (reply: 2)
738. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
739. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
740. Protein Expression in stationary phase - (reply: 11)
741. Protein Smearing - (reply: 2)
742. Western blot for muscle - (reply: 2)
743. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
744. Identify the band in western blot - (reply: 5)
745. What kind of post-translational modification do you think are these? - (reply: 2)
746. Need help with Caspase WB - (reply: 2)
747. to keep sds-pa gels - (reply: 2)
748. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
749. Ponceau staining - Appearence of bands (reply: 1)
750. Detecting phosphorylated Erk1/2 - (reply: 6)

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