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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
722. Western not working anymore - (reply: 15)
723. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
724. BN-PAGE possible with Tris-glycine - (reply: 1)
725. Western few questions - (reply: 1)
726. thiourea in 2D page - What's it for? (reply: 1)
727. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
728. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
729. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
730. Antibody works in immunofluorescence, but not in WB - (reply: 4)
731. B-actin as western blot -control - (reply: 4)
732. horrific western blots , please help - (reply: 3)
733. Trouble with actin, HA and CCND1 :(( - (reply: 1)
734. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
735. Lower MW bands than expected. - (reply: 1)
736. Question about reducing agent (DTT) - (reply: 1)
737. Collagen II western blotting - (reply: 1)
738. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
739. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
740. NUCLEAR PROTEIN PREPARATION - (reply: 1)
741. Lost protein from blot? - (reply: 1)
742. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
743. No SDS in gels - (reply: 12)
744. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
745. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
746. Sample loading buffer turning yellow. - (reply: 3)
747. western bands look funny - (reply: 5)
748. IgG SDS-PAGE - (reply: 1)
749. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
750. wet or semi-dry transfer for low MW proteins - (reply: 4)

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