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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. MES buffer turned yellow - (reply: 5)
722. western blot woes - (reply: 2)
723. Size larger than expected - totally boggled - (reply: 2)
724. Ponceau Red Staining Problem - (reply: 2)
725. SDS-PAGE Second interface - (reply: 3)
726. biorad's 30% bis/acrylamide storage - (reply: 2)
727. protein concentration per well - (reply: 6)
728. dot blot - Problem (reply: 1)
729. Clear native PAGE - (reply: 1)
730. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
731. Weak western signal for help - (reply: 13)
732. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
733. Western not working anymore - (reply: 15)
734. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
735. BN-PAGE possible with Tris-glycine - (reply: 1)
736. Western few questions - (reply: 1)
737. thiourea in 2D page - What's it for? (reply: 1)
738. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
739. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
740. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
741. Antibody works in immunofluorescence, but not in WB - (reply: 4)
742. B-actin as western blot -control - (reply: 4)
743. horrific western blots , please help - (reply: 3)
744. Trouble with actin, HA and CCND1 :(( - (reply: 1)
745. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
746. Lower MW bands than expected. - (reply: 1)
747. Question about reducing agent (DTT) - (reply: 1)
748. Collagen II western blotting - (reply: 1)
749. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
750. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)

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