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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. "Diffuesed" bands of higher molecular weight - (reply: 6)
722. Dimeric form of a protein? - I want my monomer! (reply: 2)
723. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
724. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
725. how to get rabbit antibody for Western blotting - (reply: 2)
726. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
727. Not sure of what % to use for gels - (reply: 6)
728. Protein Size - (reply: 3)
729. no separation of MW marker on gel - (reply: 1)
730. Transfer basic protein to PVDF - (reply: 1)
731. Expired pre-cast gels - (reply: 4)
732. LDS sample buffer in 95C - (reply: 2)
733. disappearing high molecular protein and marker,thanks - (reply: 1)
734. Use of cold milk??? - Western blot (reply: 2)
735. Antibody compatibility between different species - (reply: 4)
736. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 4)
737. Wrong bands on the blot - (reply: 4)
738. Protein drag in IEF-WB - (reply: 3)
739. SDS-PAGE gel staining problem - (reply: 1)
740. sample buffer pH - its effect?? (reply: 12)
741. SDS-Page and DMSO - (reply: 2)
742. Help with Western Blotting of Keratin Proteins - (reply: 2)
743. Problems detecting my band! - (reply: 2)
744. How many times can I reuse Ponceau S? - (reply: 3)
745. sds-page problems - polymerization of acrylamide (reply: 5)
746. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
747. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
748. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
749. Interpreting a gel - (reply: 3)
750. Plant isoenzymes! - (reply: 7)

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