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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. thiourea in 2D page - What's it for? (reply: 1)
722. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
723. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
724. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
725. Antibody works in immunofluorescence, but not in WB - (reply: 4)
726. B-actin as western blot -control - (reply: 4)
727. horrific western blots , please help - (reply: 3)
728. Trouble with actin, HA and CCND1 :(( - (reply: 1)
729. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
730. Lower MW bands than expected. - (reply: 1)
731. Question about reducing agent (DTT) - (reply: 1)
732. Collagen II western blotting - (reply: 1)
733. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
734. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
735. NUCLEAR PROTEIN PREPARATION - (reply: 1)
736. Lost protein from blot? - (reply: 1)
737. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
738. No SDS in gels - (reply: 12)
739. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
740. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
741. Sample loading buffer turning yellow. - (reply: 3)
742. western bands look funny - (reply: 5)
743. IgG SDS-PAGE - (reply: 1)
744. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
745. wet or semi-dry transfer for low MW proteins - (reply: 4)
746. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
747. quantifying "ghost" bands - (reply: 1)
748. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
749. Western blot running and loading samples - (reply: 6)
750. problems with His tagged protein western blots - (reply: 3)

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