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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. horrific western blots , please help - (reply: 3)
722. Trouble with actin, HA and CCND1 :(( - (reply: 1)
723. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
724. Lower MW bands than expected. - (reply: 1)
725. Question about reducing agent (DTT) - (reply: 1)
726. Collagen II western blotting - (reply: 1)
727. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
728. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
729. NUCLEAR PROTEIN PREPARATION - (reply: 1)
730. Lost protein from blot? - (reply: 1)
731. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
732. No SDS in gels - (reply: 12)
733. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
734. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
735. Sample loading buffer turning yellow. - (reply: 3)
736. western bands look funny - (reply: 5)
737. IgG SDS-PAGE - (reply: 1)
738. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
739. wet or semi-dry transfer for low MW proteins - (reply: 4)
740. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
741. quantifying "ghost" bands - (reply: 1)
742. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
743. Western blot running and loading samples - (reply: 6)
744. problems with His tagged protein western blots - (reply: 3)
745. Springerlink--Zymography - (reply: 3)
746. Methanol vs Isopropanol in fixing solution - (reply: 1)
747. Protein aggregates SDS-PAGE - (reply: 5)
748. probably DNA is disturbing the quantification of my sample - (reply: 3)
749. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
750. Optimising antibody concentration - hints and tips (reply: 1)

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