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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. Fermentas prestained protein marker issue! - (reply: 7)
722. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
723. Invitrogen NuPAGE Sample buffer - (reply: 1)
724. western exposure one part of band darker - (reply: 6)
725. non-specific bands - (reply: 5)
726. Crazy looking BN-PAGE gel - No, really. (reply: 5)
727. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
728. bands of protein seems like it did not move - (reply: 2)
729. downshifted band - (reply: 4)
730. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
731. Western Troubleshooting Help, please! - (reply: 3)
732. Re-use membrane in Western Blot - (reply: 3)
733. NP40 or Triton as membrane protein detergent - (reply: 1)
734. Dot blot- great, western blot - no signal - (reply: 3)
735. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
736. Problem in transfer high MW protein - (reply: 5)
737. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
738. dimerization or aggregation of my protein! - (reply: 3)
739. basic question in SDS and western blot - (reply: 1)
740. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
741. Transfer problems - (reply: 1)
742. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
743. Stripping temperature - have no 55C water bath (reply: 1)
744. Nonspecificity of anti-flag - (reply: 2)
745. Blocking buffer - (reply: 7)
746. Detection of phospho-proteins - (reply: 1)
747. light chain - (reply: 5)
748. ugly merged bands in western blot - (reply: 3)
749. Western Blot Data Analysis - (reply: 2)
750. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)

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