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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. Re-Imaging without Stripping - (reply: 4)
722. MES buffer turned yellow - (reply: 5)
723. western blot woes - (reply: 2)
724. Size larger than expected - totally boggled - (reply: 2)
725. Ponceau Red Staining Problem - (reply: 2)
726. SDS-PAGE Second interface - (reply: 3)
727. biorad's 30% bis/acrylamide storage - (reply: 2)
728. protein concentration per well - (reply: 6)
729. dot blot - Problem (reply: 1)
730. Clear native PAGE - (reply: 1)
731. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
732. Weak western signal for help - (reply: 13)
733. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
734. Western not working anymore - (reply: 15)
735. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
736. BN-PAGE possible with Tris-glycine - (reply: 1)
737. Western few questions - (reply: 1)
738. thiourea in 2D page - What's it for? (reply: 1)
739. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
740. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
741. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
742. Antibody works in immunofluorescence, but not in WB - (reply: 4)
743. B-actin as western blot -control - (reply: 4)
744. horrific western blots , please help - (reply: 3)
745. Trouble with actin, HA and CCND1 :(( - (reply: 1)
746. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
747. Lower MW bands than expected. - (reply: 1)
748. Question about reducing agent (DTT) - (reply: 1)
749. Collagen II western blotting - (reply: 1)
750. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)

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