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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. protein concentration per well - (reply: 6)
722. dot blot - Problem (reply: 1)
723. Clear native PAGE - (reply: 1)
724. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
725. Weak western signal for help - (reply: 13)
726. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
727. Western not working anymore - (reply: 15)
728. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
729. BN-PAGE possible with Tris-glycine - (reply: 1)
730. Western few questions - (reply: 1)
731. thiourea in 2D page - What's it for? (reply: 1)
732. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
733. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
734. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
735. Antibody works in immunofluorescence, but not in WB - (reply: 4)
736. B-actin as western blot -control - (reply: 4)
737. horrific western blots , please help - (reply: 3)
738. Trouble with actin, HA and CCND1 :(( - (reply: 1)
739. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
740. Lower MW bands than expected. - (reply: 1)
741. Question about reducing agent (DTT) - (reply: 1)
742. Collagen II western blotting - (reply: 1)
743. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
744. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
745. NUCLEAR PROTEIN PREPARATION - (reply: 1)
746. Lost protein from blot? - (reply: 1)
747. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
748. No SDS in gels - (reply: 12)
749. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
750. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)

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