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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. dot blot - Problem (reply: 1)
722. Clear native PAGE - (reply: 1)
723. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
724. Weak western signal for help - (reply: 13)
725. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
726. Western not working anymore - (reply: 15)
727. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
728. BN-PAGE possible with Tris-glycine - (reply: 1)
729. Western few questions - (reply: 1)
730. thiourea in 2D page - What's it for? (reply: 1)
731. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
732. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
733. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
734. Antibody works in immunofluorescence, but not in WB - (reply: 4)
735. B-actin as western blot -control - (reply: 4)
736. horrific western blots , please help - (reply: 3)
737. Trouble with actin, HA and CCND1 :(( - (reply: 1)
738. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
739. Lower MW bands than expected. - (reply: 1)
740. Question about reducing agent (DTT) - (reply: 1)
741. Collagen II western blotting - (reply: 1)
742. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
743. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
744. NUCLEAR PROTEIN PREPARATION - (reply: 1)
745. Lost protein from blot? - (reply: 1)
746. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
747. No SDS in gels - (reply: 12)
748. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
749. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
750. Sample loading buffer turning yellow. - (reply: 3)

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