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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
722. bands of protein seems like it did not move - (reply: 2)
723. downshifted band - (reply: 4)
724. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
725. Western Troubleshooting Help, please! - (reply: 3)
726. Re-use membrane in Western Blot - (reply: 3)
727. NP40 or Triton as membrane protein detergent - (reply: 1)
728. Dot blot- great, western blot - no signal - (reply: 3)
729. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
730. Problem in transfer high MW protein - (reply: 5)
731. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
732. dimerization or aggregation of my protein! - (reply: 3)
733. basic question in SDS and western blot - (reply: 1)
734. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
735. Transfer problems - (reply: 1)
736. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
737. Stripping temperature - have no 55C water bath (reply: 1)
738. Nonspecificity of anti-flag - (reply: 2)
739. Blocking buffer - (reply: 7)
740. Detection of phospho-proteins - (reply: 1)
741. light chain - (reply: 5)
742. ugly merged bands in western blot - (reply: 3)
743. Western Blot Data Analysis - (reply: 2)
744. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
745. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
746. Protein Expression in stationary phase - (reply: 11)
747. Protein Smearing - (reply: 2)
748. Western blot for muscle - (reply: 2)
749. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
750. Identify the band in western blot - (reply: 5)

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