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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
722. Strength of actin signal - How dark should the band be? (reply: 5)
723. Immunoprecipitation - (reply: 2)
724. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
725. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
726. SEmi-dry transfer blotting - (reply: 1)
727. Whole cell lysis of fibroblasts - (reply: 1)
728. SDS-PAGE: Strange Running - (reply: 8)
729. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
730. Different TBST pH's for WB incubation, why? - (reply: 1)
731. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
732. stability of the stripping buffer - (reply: 2)
733. small proteins have run ahead of tracking dye - (reply: 1)
734. cytoplasmic and nuclear extraction - (reply: 2)
735. Equal Protein Quantity - Loading onto Well (reply: 6)
736. Help, odd pattern - pic included - (reply: 3)
737. Fermentas prestained protein marker issue! - (reply: 7)
738. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
739. Invitrogen NuPAGE Sample buffer - (reply: 1)
740. western exposure one part of band darker - (reply: 6)
741. non-specific bands - (reply: 5)
742. Crazy looking BN-PAGE gel - No, really. (reply: 5)
743. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
744. bands of protein seems like it did not move - (reply: 2)
745. downshifted band - (reply: 4)
746. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
747. Western Troubleshooting Help, please! - (reply: 3)
748. Re-use membrane in Western Blot - (reply: 3)
749. NP40 or Triton as membrane protein detergent - (reply: 2)
750. Dot blot- great, western blot - no signal - (reply: 3)

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