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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
721. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
722. Identify the band in western blot - (reply: 5)
723. What kind of post-translational modification do you think are these? - (reply: 2)
724. Need help with Caspase WB - (reply: 2)
725. to keep sds-pa gels - (reply: 2)
726. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
727. Ponceau staining - Appearence of bands (reply: 1)
728. Detecting phosphorylated Erk1/2 - (reply: 6)
729. Detecting BDNF with WB... - troubleshooting... (reply: 2)
730. western blot using culture media - (reply: 4)
731. Re-inserting marker into scanned WB - (reply: 1)
732. pre stained marker issues - (reply: 2)
733. Functions of BSA and Tween 20 in western blotting - (reply: 2)
734. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
735. Western with conjugated antibody? - (reply: 2)
736. Protein bands on the bottom of the gel - (reply: 5)
737. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
738. Tube gel IEF problems - Polymerization... (reply: 2)
739. Cannot detect GFP using GFP antibody - (reply: 10)
740. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
741. Acrylamide/bis stock expiration? - (reply: 2)
742. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
743. Incubation with washing buffer only? - (reply: 4)
744. Ponceau Re-staining - (reply: 1)
745. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
746. color of 5 times SDS loading dye - (reply: 1)
747. Protein concentration and - (reply: 3)
748. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
749. Multiple antibodies give no signal - (reply: 10)
750. Problem with protein samples - No bands detected at all on gel (reply: 2)

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