Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Smeared transfer on nitrocellulose membrane - (reply: 2)
452. blocking and primary antibody in different buffers? - (reply: 1)
453. Same non specific band for different target proteins - (reply: 1)
454. Types of 96-well plates used for BCA protein quantification - (reply: 1)
455. Dot blot halo - (reply: 4)
456. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
457. Western blot and isoelectric point - (reply: 1)
458. Western Blot Developing Cassette - (reply: 1)
459. Pull down assay and Co- IP experiment - (reply: 6)
460. Trouble detecting Histaq - (reply: 4)
461. Western gels running super slow! - (reply: 3)
462. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
463. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
464. WB parametric or non-parametric - (reply: 6)
465. Trouble with the GFP signal - (reply: 1)
466. Intern Needing Help - (reply: 3)
467. SDS-Electrophoresis, Dye color change - (reply: 1)
468. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
469. Trouble with equal loading - (reply: 8)
470. how many times striping can b done of the blot - (reply: 9)
471. SDS-PAGE of negatively charged protein - (reply: 4)
472. Protection Lanes? - (reply: 4)
473. Tie-2 westerns high background black lanes - (reply: 13)
474. BSA concentration for blocking - (reply: 5)
475. Identically loaded western bands loose intensity from left to right - (reply: 3)
476. eIF4G western blot - (reply: 3)
477. smear bands in western blot - (reply: 12)
478. Huge background with HT22 cells phophoERK antibody - (reply: 3)
479. How to quantify the protein amount for serum samples ? - (reply: 2)
480. Huge background with HT22 cells phophoERK antibody - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37