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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Intern Needing Help - (reply: 3)
452. SDS-Electrophoresis, Dye color change - (reply: 1)
453. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
454. Trouble with equal loading - (reply: 8)
455. how many times striping can b done of the blot - (reply: 9)
456. SDS-PAGE of negatively charged protein - (reply: 4)
457. Protection Lanes? - (reply: 4)
458. Tie-2 westerns high background black lanes - (reply: 13)
459. BSA concentration for blocking - (reply: 5)
460. Identically loaded western bands loose intensity from left to right - (reply: 3)
461. eIF4G western blot - (reply: 3)
462. smear bands in western blot - (reply: 12)
463. Huge background with HT22 cells phophoERK antibody - (reply: 3)
464. How to quantify the protein amount for serum samples ? - (reply: 2)
465. Huge background with HT22 cells phophoERK antibody - (reply: 1)
466. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
467. horizontal smear? - (reply: 2)
468. BSA versus milk in blocking and antibody incubation - (reply: 5)
469. Western blots are 'semi' quantitative? - (reply: 12)
470. bands fuse together - (reply: 1)
471. Phospho-protein detection - (reply: 2)
472. Help on using different antibodies in the same membrane - (reply: 8)
473. weird query for Western Blotting - (reply: 2)
474. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
475. Quick/One-step/automated Western Blot - (reply: 8)
476. Amount of antibody used in Western Blot - (reply: 1)
477. WB Transfer: Voltage vs. Current - (reply: 7)
478. Large protein blot ~400kDa - (reply: 3)
479. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
480. Stripping buffer for nitrocellulose membrane - (reply: 1)

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