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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Strange bands and large smear in western blot - (reply: 4)
452. Freezing proteins at -80 - (reply: 1)
453. Tris glycine SDS buffer prep pH error - (reply: 2)
454. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
455. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
456. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
457. problems with negative control - (reply: 2)
458. urgent-W.B visualisation on kodak film - (reply: 2)
459. NP 40 lysis buffer - (reply: 1)
460. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
461. Dark western blot film - (reply: 12)
462. how to get red of high secondary signal - (reply: 4)
463. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
464. Detecting phosphoproteins - different results when samples rerun (reply: 1)
465. Is loading buffer ok? - (reply: 1)
466. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
467. Antibody vs Antigen concentration effect - (reply: 3)
468. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
469. SDS PAGE smearing of bands - (reply: 2)
470. Fuzzy bands in western blot - (reply: 2)
471. I am desperated with my WB!! - (reply: 2)
472. storing SDS-PAGE gels for W.Blot - (reply: 7)
473. trouble detecting phospho-proteins - (reply: 2)
474. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
475. BSA + DTT versus -DTT - (reply: 1)
476. WB - Transferring REALLY big proteins - (reply: 2)
477. Over-stripping blot? - (reply: 5)
478. Oscillation on my Western Blot results - (reply: 3)
479. HELP!!! Non-specific binding on my membrane - (reply: 7)
480. Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)

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