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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Western Blot Developing Cassette - (reply: 1)
452. Pull down assay and Co- IP experiment - (reply: 6)
453. Trouble detecting Histaq - (reply: 4)
454. Western gels running super slow! - (reply: 3)
455. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
456. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
457. WB parametric or non-parametric - (reply: 6)
458. Trouble with the GFP signal - (reply: 1)
459. Intern Needing Help - (reply: 3)
460. SDS-Electrophoresis, Dye color change - (reply: 1)
461. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
462. Trouble with equal loading - (reply: 8)
463. how many times striping can b done of the blot - (reply: 9)
464. SDS-PAGE of negatively charged protein - (reply: 4)
465. Protection Lanes? - (reply: 4)
466. Tie-2 westerns high background black lanes - (reply: 13)
467. BSA concentration for blocking - (reply: 5)
468. Identically loaded western bands loose intensity from left to right - (reply: 3)
469. eIF4G western blot - (reply: 3)
470. smear bands in western blot - (reply: 12)
471. Huge background with HT22 cells phophoERK antibody - (reply: 3)
472. How to quantify the protein amount for serum samples ? - (reply: 2)
473. Huge background with HT22 cells phophoERK antibody - (reply: 1)
474. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
475. horizontal smear? - (reply: 2)
476. BSA versus milk in blocking and antibody incubation - (reply: 5)
477. Western blots are 'semi' quantitative? - (reply: 12)
478. bands fuse together - (reply: 1)
479. Phospho-protein detection - (reply: 2)
480. Help on using different antibodies in the same membrane - (reply: 8)

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