Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. how many times striping can b done of the blot - (reply: 9)
452. SDS-PAGE of negatively charged protein - (reply: 4)
453. Protection Lanes? - (reply: 4)
454. Tie-2 westerns high background black lanes - (reply: 13)
455. BSA concentration for blocking - (reply: 5)
456. Identically loaded western bands loose intensity from left to right - (reply: 3)
457. eIF4G western blot - (reply: 3)
458. smear bands in western blot - (reply: 12)
459. Huge background with HT22 cells phophoERK antibody - (reply: 3)
460. How to quantify the protein amount for serum samples ? - (reply: 2)
461. Huge background with HT22 cells phophoERK antibody - (reply: 1)
462. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
463. horizontal smear? - (reply: 2)
464. BSA versus milk in blocking and antibody incubation - (reply: 5)
465. Western blots are 'semi' quantitative? - (reply: 12)
466. bands fuse together - (reply: 1)
467. Phospho-protein detection - (reply: 2)
468. Help on using different antibodies in the same membrane - (reply: 8)
469. weird query for Western Blotting - (reply: 2)
470. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
471. Quick/One-step/automated Western Blot - (reply: 8)
472. Amount of antibody used in Western Blot - (reply: 1)
473. WB Transfer: Voltage vs. Current - (reply: 7)
474. Large protein blot ~400kDa - (reply: 3)
475. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
476. Stripping buffer for nitrocellulose membrane - (reply: 1)
477. PAGE for Separation of DNA - (reply: 3)
478. Western Blotting of Proteins less than 10 kDa - (reply: 1)
479. dot blot - (reply: 7)
480. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36