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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Trouble with the GFP signal - (reply: 1)
452. Intern Needing Help - (reply: 3)
453. SDS-Electrophoresis, Dye color change - (reply: 1)
454. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
455. Trouble with equal loading - (reply: 8)
456. how many times striping can b done of the blot - (reply: 9)
457. SDS-PAGE of negatively charged protein - (reply: 4)
458. Protection Lanes? - (reply: 4)
459. Tie-2 westerns high background black lanes - (reply: 13)
460. BSA concentration for blocking - (reply: 5)
461. Identically loaded western bands loose intensity from left to right - (reply: 3)
462. eIF4G western blot - (reply: 3)
463. smear bands in western blot - (reply: 12)
464. Huge background with HT22 cells phophoERK antibody - (reply: 3)
465. How to quantify the protein amount for serum samples ? - (reply: 2)
466. Huge background with HT22 cells phophoERK antibody - (reply: 1)
467. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
468. horizontal smear? - (reply: 2)
469. BSA versus milk in blocking and antibody incubation - (reply: 5)
470. Western blots are 'semi' quantitative? - (reply: 12)
471. bands fuse together - (reply: 1)
472. Phospho-protein detection - (reply: 2)
473. Help on using different antibodies in the same membrane - (reply: 8)
474. weird query for Western Blotting - (reply: 2)
475. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
476. Quick/One-step/automated Western Blot - (reply: 8)
477. Amount of antibody used in Western Blot - (reply: 1)
478. WB Transfer: Voltage vs. Current - (reply: 7)
479. Large protein blot ~400kDa - (reply: 3)
480. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)

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