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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. BSA vs Milk - (reply: 2)
452. Western Blot no target bands, show Non-specific bands - (reply: 5)
453. how critical is the pH of tbst? - (reply: 2)
454. Fc fusion protein smearing on SDS PAGE - (reply: 2)
455. what do you recommend for Semi-Dry unit - (reply: 2)
456. Bands disappear after longer exposure - (reply: 4)
457. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
458. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
459. Antibody Optimization - (reply: 2)
460. Protein agregation in WB samples - (reply: 3)
461. Same Non-specific bands show up for different Abs!! - (reply: 7)
462. what caused bubble band of my WB? - (reply: 4)
463. blotting detection - (reply: 3)
464. Max amount of protein - (reply: 4)
465. Areas of membrane bleached - (reply: 1)
466. asking suggestions for western blot - (reply: 3)
467. Can i apply primary antibody for two nights instead of one? - (reply: 3)
468. Strange bands and large smear in western blot - (reply: 4)
469. Freezing proteins at -80 - (reply: 1)
470. Tris glycine SDS buffer prep pH error - (reply: 2)
471. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
472. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
473. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
474. problems with negative control - (reply: 2)
475. urgent-W.B visualisation on kodak film - (reply: 2)
476. NP 40 lysis buffer - (reply: 1)
477. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
478. Dark western blot film - (reply: 12)
479. how to get red of high secondary signal - (reply: 4)
480. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)

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