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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Loading controls - (reply: 1)
452. Quantity of protein to charge - (reply: 1)
453. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
454. Tris-Hcl becomes cloudy. - (reply: 3)
455. Can FLAG IP, but cannot detect with Western - (reply: 4)
456. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
457. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
458. Native PAGE, interaction of proteins, validity - (reply: 4)
459. Smeared transfer on nitrocellulose membrane - (reply: 2)
460. blocking and primary antibody in different buffers? - (reply: 1)
461. Same non specific band for different target proteins - (reply: 1)
462. Types of 96-well plates used for BCA protein quantification - (reply: 1)
463. Dot blot halo - (reply: 4)
464. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
465. Western blot and isoelectric point - (reply: 1)
466. Western Blot Developing Cassette - (reply: 1)
467. Pull down assay and Co- IP experiment - (reply: 6)
468. Trouble detecting Histaq - (reply: 4)
469. Western gels running super slow! - (reply: 3)
470. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
471. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
472. WB parametric or non-parametric - (reply: 6)
473. Trouble with the GFP signal - (reply: 1)
474. Intern Needing Help - (reply: 3)
475. SDS-Electrophoresis, Dye color change - (reply: 1)
476. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
477. Trouble with equal loading - (reply: 8)
478. how many times striping can b done of the blot - (reply: 9)
479. SDS-PAGE of negatively charged protein - (reply: 4)
480. Protection Lanes? - (reply: 4)

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