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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Protection Lanes? - (reply: 4)
452. Tie-2 westerns high background black lanes - (reply: 13)
453. BSA concentration for blocking - (reply: 5)
454. Identically loaded western bands loose intensity from left to right - (reply: 3)
455. eIF4G western blot - (reply: 3)
456. smear bands in western blot - (reply: 12)
457. Huge background with HT22 cells phophoERK antibody - (reply: 3)
458. How to quantify the protein amount for serum samples ? - (reply: 2)
459. Huge background with HT22 cells phophoERK antibody - (reply: 1)
460. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
461. horizontal smear? - (reply: 2)
462. BSA versus milk in blocking and antibody incubation - (reply: 5)
463. Western blots are 'semi' quantitative? - (reply: 12)
464. bands fuse together - (reply: 1)
465. Phospho-protein detection - (reply: 2)
466. Help on using different antibodies in the same membrane - (reply: 8)
467. weird query for Western Blotting - (reply: 2)
468. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
469. Quick/One-step/automated Western Blot - (reply: 8)
470. Amount of antibody used in Western Blot - (reply: 1)
471. WB Transfer: Voltage vs. Current - (reply: 7)
472. Large protein blot ~400kDa - (reply: 3)
473. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
474. Stripping buffer for nitrocellulose membrane - (reply: 1)
475. PAGE for Separation of DNA - (reply: 3)
476. Western Blotting of Proteins less than 10 kDa - (reply: 1)
477. dot blot - (reply: 7)
478. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
479. Internal Standard for western blot - (reply: 1)
480. Sox-2 antibody detection using Western - (reply: 10)

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