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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. SDS-Electrophoresis, Dye color change - (reply: 1)
452. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
453. Trouble with equal loading - (reply: 8)
454. how many times striping can b done of the blot - (reply: 9)
455. SDS-PAGE of negatively charged protein - (reply: 4)
456. Protection Lanes? - (reply: 4)
457. Tie-2 westerns high background black lanes - (reply: 13)
458. BSA concentration for blocking - (reply: 5)
459. Identically loaded western bands loose intensity from left to right - (reply: 3)
460. eIF4G western blot - (reply: 3)
461. smear bands in western blot - (reply: 12)
462. Huge background with HT22 cells phophoERK antibody - (reply: 3)
463. How to quantify the protein amount for serum samples ? - (reply: 2)
464. Huge background with HT22 cells phophoERK antibody - (reply: 1)
465. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
466. horizontal smear? - (reply: 2)
467. BSA versus milk in blocking and antibody incubation - (reply: 5)
468. Western blots are 'semi' quantitative? - (reply: 12)
469. bands fuse together - (reply: 1)
470. Phospho-protein detection - (reply: 2)
471. Help on using different antibodies in the same membrane - (reply: 8)
472. weird query for Western Blotting - (reply: 2)
473. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
474. Quick/One-step/automated Western Blot - (reply: 8)
475. Amount of antibody used in Western Blot - (reply: 1)
476. WB Transfer: Voltage vs. Current - (reply: 7)
477. Large protein blot ~400kDa - (reply: 3)
478. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
479. Stripping buffer for nitrocellulose membrane - (reply: 1)
480. PAGE for Separation of DNA - (reply: 3)

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