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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. BSA concentration for blocking - (reply: 5)
452. Identically loaded western bands loose intensity from left to right - (reply: 3)
453. eIF4G western blot - (reply: 3)
454. smear bands in western blot - (reply: 12)
455. Huge background with HT22 cells phophoERK antibody - (reply: 3)
456. How to quantify the protein amount for serum samples ? - (reply: 2)
457. Huge background with HT22 cells phophoERK antibody - (reply: 1)
458. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
459. horizontal smear? - (reply: 2)
460. BSA versus milk in blocking and antibody incubation - (reply: 5)
461. Western blots are 'semi' quantitative? - (reply: 12)
462. bands fuse together - (reply: 1)
463. Phospho-protein detection - (reply: 2)
464. Help on using different antibodies in the same membrane - (reply: 8)
465. weird query for Western Blotting - (reply: 2)
466. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
467. Quick/One-step/automated Western Blot - (reply: 8)
468. Amount of antibody used in Western Blot - (reply: 1)
469. WB Transfer: Voltage vs. Current - (reply: 7)
470. Large protein blot ~400kDa - (reply: 3)
471. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
472. Stripping buffer for nitrocellulose membrane - (reply: 1)
473. PAGE for Separation of DNA - (reply: 3)
474. Western Blotting of Proteins less than 10 kDa - (reply: 1)
475. dot blot - (reply: 7)
476. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
477. Internal Standard for western blot - (reply: 1)
478. Sox-2 antibody detection using Western - (reply: 10)
479. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
480. gH2A.X blotting problem - (reply: 1)

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