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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Tris-Hcl becomes cloudy. - (reply: 3)
452. Can FLAG IP, but cannot detect with Western - (reply: 4)
453. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
454. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
455. Native PAGE, interaction of proteins, validity - (reply: 4)
456. Smeared transfer on nitrocellulose membrane - (reply: 2)
457. blocking and primary antibody in different buffers? - (reply: 1)
458. Same non specific band for different target proteins - (reply: 1)
459. Types of 96-well plates used for BCA protein quantification - (reply: 1)
460. Dot blot halo - (reply: 4)
461. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
462. Western blot and isoelectric point - (reply: 1)
463. Western Blot Developing Cassette - (reply: 1)
464. Pull down assay and Co- IP experiment - (reply: 6)
465. Trouble detecting Histaq - (reply: 4)
466. Western gels running super slow! - (reply: 3)
467. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
468. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
469. WB parametric or non-parametric - (reply: 6)
470. Trouble with the GFP signal - (reply: 1)
471. Intern Needing Help - (reply: 3)
472. SDS-Electrophoresis, Dye color change - (reply: 1)
473. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
474. Trouble with equal loading - (reply: 8)
475. how many times striping can b done of the blot - (reply: 9)
476. SDS-PAGE of negatively charged protein - (reply: 4)
477. Protection Lanes? - (reply: 4)
478. Tie-2 westerns high background black lanes - (reply: 13)
479. BSA concentration for blocking - (reply: 5)
480. Identically loaded western bands loose intensity from left to right - (reply: 3)

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