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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Western Blot no target bands, show Non-specific bands - (reply: 5)
452. how critical is the pH of tbst? - (reply: 2)
453. Fc fusion protein smearing on SDS PAGE - (reply: 2)
454. what do you recommend for Semi-Dry unit - (reply: 2)
455. Bands disappear after longer exposure - (reply: 4)
456. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
457. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
458. Antibody Optimization - (reply: 2)
459. Protein agregation in WB samples - (reply: 3)
460. Same Non-specific bands show up for different Abs!! - (reply: 7)
461. what caused bubble band of my WB? - (reply: 4)
462. blotting detection - (reply: 3)
463. Max amount of protein - (reply: 4)
464. Areas of membrane bleached - (reply: 1)
465. asking suggestions for western blot - (reply: 3)
466. Can i apply primary antibody for two nights instead of one? - (reply: 3)
467. Strange bands and large smear in western blot - (reply: 4)
468. Freezing proteins at -80 - (reply: 1)
469. Tris glycine SDS buffer prep pH error - (reply: 2)
470. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
471. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
472. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
473. problems with negative control - (reply: 2)
474. urgent-W.B visualisation on kodak film - (reply: 2)
475. NP 40 lysis buffer - (reply: 1)
476. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
477. Dark western blot film - (reply: 12)
478. how to get red of high secondary signal - (reply: 4)
479. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
480. Detecting phosphoproteins - different results when samples rerun (reply: 1)

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