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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
451. Dot blot halo - (reply: 4)
452. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
453. Western blot and isoelectric point - (reply: 1)
454. Western Blot Developing Cassette - (reply: 1)
455. Pull down assay and Co- IP experiment - (reply: 6)
456. Trouble detecting Histaq - (reply: 4)
457. Western gels running super slow! - (reply: 3)
458. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
459. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
460. WB parametric or non-parametric - (reply: 6)
461. Trouble with the GFP signal - (reply: 1)
462. Intern Needing Help - (reply: 3)
463. SDS-Electrophoresis, Dye color change - (reply: 1)
464. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
465. Trouble with equal loading - (reply: 8)
466. how many times striping can b done of the blot - (reply: 9)
467. SDS-PAGE of negatively charged protein - (reply: 4)
468. Protection Lanes? - (reply: 4)
469. Tie-2 westerns high background black lanes - (reply: 13)
470. BSA concentration for blocking - (reply: 5)
471. Identically loaded western bands loose intensity from left to right - (reply: 3)
472. eIF4G western blot - (reply: 3)
473. smear bands in western blot - (reply: 12)
474. Huge background with HT22 cells phophoERK antibody - (reply: 3)
475. How to quantify the protein amount for serum samples ? - (reply: 2)
476. Huge background with HT22 cells phophoERK antibody - (reply: 1)
477. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
478. horizontal smear? - (reply: 2)
479. BSA versus milk in blocking and antibody incubation - (reply: 5)
480. Western blots are 'semi' quantitative? - (reply: 12)

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