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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Protein concentration and - (reply: 3)
842. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
843. Multiple antibodies give no signal - (reply: 10)
844. Problem with protein samples - No bands detected at all on gel (reply: 2)
845. using cell lysate to run native gel? - (reply: 1)
846. Bradford Query - (reply: 5)
847. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
848. About GFP- tagged proteins in western blot - (reply: 1)
849. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
850. PVP-40 for blocking? - Instead of milk (reply: 2)
851. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
852. How to pase my Co-IP over the weekend? - (reply: 1)
853. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
854. Protein Sample Preparation - Western Bloting (reply: 1)
855. No signal at all - (reply: 16)
856. Centrifuge after lysing - (reply: 5)
857. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
858. "Diffuesed" bands of higher molecular weight - (reply: 6)
859. Dimeric form of a protein? - I want my monomer! (reply: 2)
860. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
861. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
862. how to get rabbit antibody for Western blotting - (reply: 2)
863. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
864. Not sure of what % to use for gels - (reply: 6)
865. Protein Size - (reply: 3)
866. no separation of MW marker on gel - (reply: 1)
867. Transfer basic protein to PVDF - (reply: 1)
868. Expired pre-cast gels - (reply: 4)
869. LDS sample buffer in 95C - (reply: 2)
870. disappearing high molecular protein and marker,thanks - (reply: 1)

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