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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. "Diffuesed" bands of higher molecular weight - (reply: 6)
842. Dimeric form of a protein? - I want my monomer! (reply: 2)
843. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
844. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
845. how to get rabbit antibody for Western blotting - (reply: 2)
846. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
847. Not sure of what % to use for gels - (reply: 6)
848. Protein Size - (reply: 3)
849. no separation of MW marker on gel - (reply: 1)
850. Transfer basic protein to PVDF - (reply: 1)
851. Expired pre-cast gels - (reply: 4)
852. LDS sample buffer in 95C - (reply: 2)
853. disappearing high molecular protein and marker,thanks - (reply: 1)
854. Use of cold milk??? - Western blot (reply: 2)
855. Antibody compatibility between different species - (reply: 4)
856. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)
857. Wrong bands on the blot - (reply: 4)
858. Protein drag in IEF-WB - (reply: 3)
859. SDS-PAGE gel staining problem - (reply: 1)
860. sample buffer pH - its effect?? (reply: 12)
861. SDS-Page and DMSO - (reply: 2)
862. Help with Western Blotting of Keratin Proteins - (reply: 2)
863. Problems detecting my band! - (reply: 2)
864. How many times can I reuse Ponceau S? - (reply: 3)
865. sds-page problems - polymerization of acrylamide (reply: 5)
866. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
867. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
868. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
869. Interpreting a gel - (reply: 3)
870. Plant isoenzymes! - (reply: 7)

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