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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Protein size and gel percentage - (reply: 7)
842. Bands visible in membrane but not in the film - ?? (reply: 10)
843. non desired brigther bands - (reply: 7)
844. Dimer or monomer - (reply: 11)
845. Protein precipitation by TCA acid + acetone - (reply: 4)
846. Non Reducing SDS protein ladder?? - (reply: 4)
847. Protein Free Blocking Buffers - (reply: 1)
848. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
849. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
850. SDS-Page issue - (reply: 5)
851. Fluorescent visualization of Western blot - (reply: 2)
852. MEthanol in NC membrane - (reply: 7)
853. Pre stained markers - (reply: 4)
854. Laminin 5 antibody - (reply: 1)
855. Molecular weight marker - got different pattern! (reply: 13)
856. SDS ladder - which type works better? (reply: 3)
857. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
858. the sensitive amount of protein for western blotting - (reply: 3)
859. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
860. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
861. How to determine final amount of 20 ng protein per WB well - (reply: 8)
862. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
863. nonreducing antibody in reducing condition - (reply: 1)
864. High Molecular weight protein separation - (reply: 2)
865. Secreted protein for loading control - (reply: 2)
866. Only borders stained in Silver!!! - (reply: 7)
867. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
868. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
869. bromophenol blue - (reply: 10)
870. Native Page transfer/detection problems - (reply: 1)

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