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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
842. Acrylamide/bis stock expiration? - (reply: 2)
843. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
844. Incubation with washing buffer only? - (reply: 4)
845. Ponceau Re-staining - (reply: 1)
846. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
847. color of 5 times SDS loading dye - (reply: 1)
848. Protein concentration and - (reply: 3)
849. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
850. Multiple antibodies give no signal - (reply: 10)
851. Problem with protein samples - No bands detected at all on gel (reply: 2)
852. using cell lysate to run native gel? - (reply: 1)
853. Bradford Query - (reply: 5)
854. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
855. About GFP- tagged proteins in western blot - (reply: 1)
856. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
857. PVP-40 for blocking? - Instead of milk (reply: 2)
858. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
859. How to pase my Co-IP over the weekend? - (reply: 1)
860. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
861. Protein Sample Preparation - Western Bloting (reply: 1)
862. No signal at all - (reply: 16)
863. Centrifuge after lysing - (reply: 5)
864. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
865. "Diffuesed" bands of higher molecular weight - (reply: 6)
866. Dimeric form of a protein? - I want my monomer! (reply: 2)
867. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
868. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
869. how to get rabbit antibody for Western blotting - (reply: 2)
870. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)

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