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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
842. Primary Antibody for SPDEF - (reply: 1)
843. Detecting Patterns of phosphorylated protein - (reply: 1)
844. Sample Diffusion Before Applying Current - (reply: 4)
845. IP and western blot - (reply: 5)
846. Semi-dry transfer conditions - Volts or Amps (reply: 1)
847. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
848. Destain after PoneauS - (reply: 6)
849. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
850. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
851. Variability in GAPDH - (reply: 5)
852. nitrocellulose or PVDF - (reply: 7)
853. crappy bands after developing - (reply: 3)
854. cloudy gel - (reply: 2)
855. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
856. Beginner Question - (reply: 6)
857. What's the problem with this SDS-PAGE? - (reply: 8)
858. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
859. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
860. Stripping question - (reply: 3)
861. Smears after transfer - (reply: 2)
862. Accidentally Froze Transfer Buffer - (reply: 1)
863. ImageJ analysis on IFs - (reply: 11)
864. Equal loading for Supernatant western blots??? - (reply: 1)
865. Ponceau Staining Question - (reply: 1)
866. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
867. bubbles between gel and glass plate - (reply: 9)
868. Want to detect oligomers - Need advice on native PAGE (reply: 2)
869. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
870. Primary antibody incubation length - (reply: 3)

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