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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Dimer or monomer - (reply: 11)
842. Protein precipitation by TCA acid + acetone - (reply: 4)
843. Non Reducing SDS protein ladder?? - (reply: 4)
844. Protein Free Blocking Buffers - (reply: 1)
845. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
846. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
847. SDS-Page issue - (reply: 5)
848. Fluorescent visualization of Western blot - (reply: 2)
849. MEthanol in NC membrane - (reply: 7)
850. Pre stained markers - (reply: 4)
851. Laminin 5 antibody - (reply: 1)
852. Molecular weight marker - got different pattern! (reply: 13)
853. SDS ladder - which type works better? (reply: 3)
854. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
855. the sensitive amount of protein for western blotting - (reply: 3)
856. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
857. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
858. How to determine final amount of 20 ng protein per WB well - (reply: 8)
859. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
860. nonreducing antibody in reducing condition - (reply: 1)
861. High Molecular weight protein separation - (reply: 2)
862. Secreted protein for loading control - (reply: 2)
863. Only borders stained in Silver!!! - (reply: 7)
864. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
865. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
866. bromophenol blue - (reply: 10)
867. Native Page transfer/detection problems - (reply: 1)
868. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
869. nuclear loading control - (reply: 2)
870. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)

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