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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
842. Protein Sample Preparation - Western Bloting (reply: 1)
843. No signal at all - (reply: 16)
844. Centrifuge after lysing - (reply: 5)
845. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
846. "Diffuesed" bands of higher molecular weight - (reply: 6)
847. Dimeric form of a protein? - I want my monomer! (reply: 2)
848. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
849. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
850. how to get rabbit antibody for Western blotting - (reply: 2)
851. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
852. Not sure of what % to use for gels - (reply: 6)
853. Protein Size - (reply: 3)
854. no separation of MW marker on gel - (reply: 1)
855. Transfer basic protein to PVDF - (reply: 1)
856. Expired pre-cast gels - (reply: 4)
857. LDS sample buffer in 95C - (reply: 2)
858. disappearing high molecular protein and marker,thanks - (reply: 1)
859. Use of cold milk??? - Western blot (reply: 2)
860. Antibody compatibility between different species - (reply: 4)
861. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)
862. Wrong bands on the blot - (reply: 4)
863. Protein drag in IEF-WB - (reply: 3)
864. SDS-PAGE gel staining problem - (reply: 1)
865. sample buffer pH - its effect?? (reply: 12)
866. SDS-Page and DMSO - (reply: 2)
867. Help with Western Blotting of Keratin Proteins - (reply: 2)
868. Problems detecting my band! - (reply: 2)
869. How many times can I reuse Ponceau S? - (reply: 3)
870. sds-page problems - polymerization of acrylamide (reply: 5)

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