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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Destain after PoneauS - (reply: 6)
842. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
843. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
844. Variability in GAPDH - (reply: 5)
845. nitrocellulose or PVDF - (reply: 7)
846. crappy bands after developing - (reply: 3)
847. cloudy gel - (reply: 2)
848. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
849. Beginner Question - (reply: 6)
850. What's the problem with this SDS-PAGE? - (reply: 8)
851. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
852. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
853. Stripping question - (reply: 3)
854. Smears after transfer - (reply: 2)
855. Accidentally Froze Transfer Buffer - (reply: 1)
856. ImageJ analysis on IFs - (reply: 11)
857. Equal loading for Supernatant western blots??? - (reply: 1)
858. Ponceau Staining Question - (reply: 1)
859. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
860. bubbles between gel and glass plate - (reply: 9)
861. Want to detect oligomers - Need advice on native PAGE (reply: 2)
862. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
863. Primary antibody incubation length - (reply: 3)
864. How to extract protein from SDS-PAGE gel? - (reply: 7)
865. colorimetry vs chemiluminescence - (reply: 1)
866. antribody gone old? - (reply: 11)
867. Yellow Laemmli sample buffer - (reply: 6)
868. Antibody Characterization - KO vs. WT or Heterologous overexpression (reply: 8)
869. does 1% SDS prevents proteases? - (reply: 8)
870. Guanidinium Chloride - (reply: 11)

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