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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Problem with protein samples - No bands detected at all on gel (reply: 2)
842. using cell lysate to run native gel? - (reply: 1)
843. Bradford Query - (reply: 5)
844. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
845. About GFP- tagged proteins in western blot - (reply: 1)
846. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
847. PVP-40 for blocking? - Instead of milk (reply: 2)
848. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
849. How to pase my Co-IP over the weekend? - (reply: 1)
850. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
851. Protein Sample Preparation - Western Bloting (reply: 1)
852. No signal at all - (reply: 16)
853. Centrifuge after lysing - (reply: 5)
854. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
855. "Diffuesed" bands of higher molecular weight - (reply: 6)
856. Dimeric form of a protein? - I want my monomer! (reply: 2)
857. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
858. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
859. how to get rabbit antibody for Western blotting - (reply: 2)
860. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
861. Not sure of what % to use for gels - (reply: 6)
862. Protein Size - (reply: 3)
863. no separation of MW marker on gel - (reply: 1)
864. Transfer basic protein to PVDF - (reply: 1)
865. Expired pre-cast gels - (reply: 4)
866. LDS sample buffer in 95C - (reply: 2)
867. disappearing high molecular protein and marker,thanks - (reply: 1)
868. Use of cold milk??? - Western blot (reply: 2)
869. Antibody compatibility between different species - (reply: 4)
870. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)

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