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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
842. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
843. How to determine final amount of 20 ng protein per WB well - (reply: 8)
844. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
845. nonreducing antibody in reducing condition - (reply: 1)
846. High Molecular weight protein separation - (reply: 2)
847. Secreted protein for loading control - (reply: 2)
848. Only borders stained in Silver!!! - (reply: 7)
849. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
850. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
851. bromophenol blue - (reply: 10)
852. Native Page transfer/detection problems - (reply: 1)
853. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
854. nuclear loading control - (reply: 2)
855. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
856. Large protein transfer problem - (reply: 8)
857. overloading GAPDH but nice band of interest - (reply: 2)
858. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
859. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
860. Quantiying bands. - Determination of protein contens (reply: 2)
861. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
862. Strange lane pattern, yes/no pattern - (reply: 3)
863. Native PAGE w. Western protein marker - (reply: 4)
864. protein stuck - (reply: 3)
865. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
866. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
867. changing colour of front dye in sds-page - (reply: 10)
868. small protein blotting - (reply: 8)
869. how stable is alpha mouse HRP? - left on the bench (reply: 2)
870. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)

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