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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. How to pase my Co-IP over the weekend? - (reply: 1)
842. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
843. Protein Sample Preparation - Western Bloting (reply: 1)
844. No signal at all - (reply: 16)
845. Centrifuge after lysing - (reply: 5)
846. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
847. "Diffuesed" bands of higher molecular weight - (reply: 6)
848. Dimeric form of a protein? - I want my monomer! (reply: 2)
849. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
850. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
851. how to get rabbit antibody for Western blotting - (reply: 2)
852. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
853. Not sure of what % to use for gels - (reply: 6)
854. Protein Size - (reply: 3)
855. no separation of MW marker on gel - (reply: 1)
856. Transfer basic protein to PVDF - (reply: 1)
857. Expired pre-cast gels - (reply: 4)
858. LDS sample buffer in 95C - (reply: 2)
859. disappearing high molecular protein and marker,thanks - (reply: 1)
860. Use of cold milk??? - Western blot (reply: 2)
861. Antibody compatibility between different species - (reply: 4)
862. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)
863. Wrong bands on the blot - (reply: 4)
864. Protein drag in IEF-WB - (reply: 3)
865. SDS-PAGE gel staining problem - (reply: 1)
866. sample buffer pH - its effect?? (reply: 12)
867. SDS-Page and DMSO - (reply: 2)
868. Help with Western Blotting of Keratin Proteins - (reply: 2)
869. Problems detecting my band! - (reply: 2)
870. How many times can I reuse Ponceau S? - (reply: 3)

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