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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Cannot detect GFP using GFP antibody - (reply: 10)
842. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
843. Acrylamide/bis stock expiration? - (reply: 2)
844. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
845. Incubation with washing buffer only? - (reply: 4)
846. Ponceau Re-staining - (reply: 1)
847. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
848. color of 5 times SDS loading dye - (reply: 1)
849. Protein concentration and - (reply: 3)
850. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
851. Multiple antibodies give no signal - (reply: 10)
852. Problem with protein samples - No bands detected at all on gel (reply: 2)
853. using cell lysate to run native gel? - (reply: 1)
854. Bradford Query - (reply: 5)
855. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
856. About GFP- tagged proteins in western blot - (reply: 1)
857. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
858. PVP-40 for blocking? - Instead of milk (reply: 2)
859. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
860. How to pase my Co-IP over the weekend? - (reply: 1)
861. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
862. Protein Sample Preparation - Western Bloting (reply: 1)
863. No signal at all - (reply: 16)
864. Centrifuge after lysing - (reply: 5)
865. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
866. "Diffuesed" bands of higher molecular weight - (reply: 6)
867. Dimeric form of a protein? - I want my monomer! (reply: 2)
868. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
869. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
870. how to get rabbit antibody for Western blotting - (reply: 2)

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