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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Incubation with washing buffer only? - (reply: 4)
842. Ponceau Re-staining - (reply: 1)
843. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
844. color of 5 times SDS loading dye - (reply: 1)
845. Protein concentration and - (reply: 3)
846. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
847. Multiple antibodies give no signal - (reply: 10)
848. Problem with protein samples - No bands detected at all on gel (reply: 2)
849. using cell lysate to run native gel? - (reply: 1)
850. Bradford Query - (reply: 5)
851. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
852. About GFP- tagged proteins in western blot - (reply: 1)
853. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
854. PVP-40 for blocking? - Instead of milk (reply: 2)
855. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
856. How to pase my Co-IP over the weekend? - (reply: 1)
857. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
858. Protein Sample Preparation - Western Bloting (reply: 1)
859. No signal at all - (reply: 16)
860. Centrifuge after lysing - (reply: 5)
861. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
862. "Diffuesed" bands of higher molecular weight - (reply: 6)
863. Dimeric form of a protein? - I want my monomer! (reply: 2)
864. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
865. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
866. how to get rabbit antibody for Western blotting - (reply: 2)
867. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
868. Not sure of what % to use for gels - (reply: 6)
869. Protein Size - (reply: 3)
870. no separation of MW marker on gel - (reply: 1)

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