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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
842. my membranes are blue! - silly mistake, what to do? (reply: 1)
843. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
844. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
845. Primary Antibody for SPDEF - (reply: 1)
846. Detecting Patterns of phosphorylated protein - (reply: 1)
847. Sample Diffusion Before Applying Current - (reply: 4)
848. IP and western blot - (reply: 5)
849. Semi-dry transfer conditions - Volts or Amps (reply: 1)
850. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
851. Destain after PoneauS - (reply: 6)
852. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
853. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
854. Variability in GAPDH - (reply: 5)
855. nitrocellulose or PVDF - (reply: 7)
856. crappy bands after developing - (reply: 3)
857. cloudy gel - (reply: 2)
858. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
859. Beginner Question - (reply: 6)
860. What's the problem with this SDS-PAGE? - (reply: 8)
861. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
862. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
863. Stripping question - (reply: 3)
864. Smears after transfer - (reply: 2)
865. Accidentally Froze Transfer Buffer - (reply: 1)
866. ImageJ analysis on IFs - (reply: 11)
867. Equal loading for Supernatant western blots??? - (reply: 1)
868. Ponceau Staining Question - (reply: 1)
869. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
870. bubbles between gel and glass plate - (reply: 9)

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