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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
841. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
842. Variability in GAPDH - (reply: 5)
843. nitrocellulose or PVDF - (reply: 7)
844. crappy bands after developing - (reply: 3)
845. cloudy gel - (reply: 2)
846. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
847. Beginner Question - (reply: 6)
848. What's the problem with this SDS-PAGE? - (reply: 8)
849. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
850. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
851. Stripping question - (reply: 3)
852. Smears after transfer - (reply: 2)
853. Accidentally Froze Transfer Buffer - (reply: 1)
854. ImageJ analysis on IFs - (reply: 11)
855. Equal loading for Supernatant western blots??? - (reply: 1)
856. Ponceau Staining Question - (reply: 1)
857. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
858. bubbles between gel and glass plate - (reply: 9)
859. Want to detect oligomers - Need advice on native PAGE (reply: 2)
860. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
861. Primary antibody incubation length - (reply: 3)
862. How to extract protein from SDS-PAGE gel? - (reply: 7)
863. colorimetry vs chemiluminescence - (reply: 1)
864. antribody gone old? - (reply: 11)
865. Yellow Laemmli sample buffer - (reply: 6)
866. Antibody Characterization - KO vs. WT or Heterologous overexpression (reply: 8)
867. does 1% SDS prevents proteases? - (reply: 8)
868. Guanidinium Chloride - (reply: 11)
869. many bands of smaller size than expected - (reply: 3)
870. how to solve fungus problem on SDS PAGE gel - (reply: 4)

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