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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. Help Stained after blocking and won't unstain!!! - (reply: 1)
692. Bands are lower than actual protein - (reply: 1)
693. Protein lysate storage - (reply: 2)
694. Is there alpha-tubulin in the nucleus? - (reply: 4)
695. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
696. No band after WB revelation - (reply: 2)
697. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
698. Expression by ICC but not by Western-why? - (reply: 3)
699. Re-Imaging without Stripping - (reply: 4)
700. MES buffer turned yellow - (reply: 5)
701. western blot woes - (reply: 2)
702. Size larger than expected - totally boggled - (reply: 2)
703. Ponceau Red Staining Problem - (reply: 2)
704. SDS-PAGE Second interface - (reply: 3)
705. biorad's 30% bis/acrylamide storage - (reply: 2)
706. protein concentration per well - (reply: 6)
707. dot blot - Problem (reply: 1)
708. Clear native PAGE - (reply: 1)
709. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
710. Weak western signal for help - (reply: 13)
711. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
712. Western not working anymore - (reply: 15)
713. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
714. BN-PAGE possible with Tris-glycine - (reply: 1)
715. Western few questions - (reply: 1)
716. thiourea in 2D page - What's it for? (reply: 1)
717. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
718. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
719. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
720. Antibody works in immunofluorescence, but not in WB - (reply: 4)

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