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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
692. Invitrogen NuPAGE Sample buffer - (reply: 1)
693. western exposure one part of band darker - (reply: 6)
694. non-specific bands - (reply: 5)
695. Crazy looking BN-PAGE gel - No, really. (reply: 5)
696. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
697. bands of protein seems like it did not move - (reply: 2)
698. downshifted band - (reply: 4)
699. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
700. Western Troubleshooting Help, please! - (reply: 3)
701. Re-use membrane in Western Blot - (reply: 3)
702. NP40 or Triton as membrane protein detergent - (reply: 1)
703. Dot blot- great, western blot - no signal - (reply: 3)
704. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
705. Problem in transfer high MW protein - (reply: 5)
706. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
707. dimerization or aggregation of my protein! - (reply: 3)
708. basic question in SDS and western blot - (reply: 1)
709. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
710. Transfer problems - (reply: 1)
711. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
712. Stripping temperature - have no 55C water bath (reply: 1)
713. Nonspecificity of anti-flag - (reply: 2)
714. Blocking buffer - (reply: 7)
715. Detection of phospho-proteins - (reply: 1)
716. light chain - (reply: 5)
717. ugly merged bands in western blot - (reply: 3)
718. Western Blot Data Analysis - (reply: 2)
719. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
720. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)

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