Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
692. NUCLEAR PROTEIN PREPARATION - (reply: 1)
693. Lost protein from blot? - (reply: 1)
694. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
695. No SDS in gels - (reply: 12)
696. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
697. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
698. Sample loading buffer turning yellow. - (reply: 3)
699. western bands look funny - (reply: 5)
700. IgG SDS-PAGE - (reply: 1)
701. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
702. wet or semi-dry transfer for low MW proteins - (reply: 4)
703. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
704. quantifying "ghost" bands - (reply: 1)
705. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
706. Western blot running and loading samples - (reply: 6)
707. problems with His tagged protein western blots - (reply: 3)
708. Springerlink--Zymography - (reply: 3)
709. Methanol vs Isopropanol in fixing solution - (reply: 1)
710. Protein aggregates SDS-PAGE - (reply: 5)
711. probably DNA is disturbing the quantification of my sample - (reply: 3)
712. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
713. Optimising antibody concentration - hints and tips (reply: 1)
714. Lanes all run together - (reply: 5)
715. Protein Markers - (reply: 1)
716. Gel Running Crooked?? Please help.. - (reply: 7)
717. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
718. Strength of actin signal - How dark should the band be? (reply: 5)
719. Immunoprecipitation - (reply: 2)
720. How can I isolate ECM protein from a 6 well dish? - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35