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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. Expression by ICC but not by Western-why? - (reply: 3)
692. Re-Imaging without Stripping - (reply: 4)
693. MES buffer turned yellow - (reply: 5)
694. western blot woes - (reply: 2)
695. Size larger than expected - totally boggled - (reply: 2)
696. Ponceau Red Staining Problem - (reply: 2)
697. SDS-PAGE Second interface - (reply: 3)
698. biorad's 30% bis/acrylamide storage - (reply: 2)
699. protein concentration per well - (reply: 6)
700. dot blot - Problem (reply: 1)
701. Clear native PAGE - (reply: 1)
702. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
703. Weak western signal for help - (reply: 13)
704. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
705. Western not working anymore - (reply: 15)
706. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
707. BN-PAGE possible with Tris-glycine - (reply: 1)
708. Western few questions - (reply: 1)
709. thiourea in 2D page - What's it for? (reply: 1)
710. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
711. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
712. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
713. Antibody works in immunofluorescence, but not in WB - (reply: 4)
714. B-actin as western blot -control - (reply: 4)
715. horrific western blots , please help - (reply: 3)
716. Trouble with actin, HA and CCND1 :(( - (reply: 1)
717. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
718. Lower MW bands than expected. - (reply: 1)
719. Question about reducing agent (DTT) - (reply: 1)
720. Collagen II western blotting - (reply: 1)

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