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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. Transfer problems - (reply: 1)
692. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
693. Stripping temperature - have no 55C water bath (reply: 1)
694. Nonspecificity of anti-flag - (reply: 2)
695. Blocking buffer - (reply: 7)
696. Detection of phospho-proteins - (reply: 1)
697. light chain - (reply: 5)
698. ugly merged bands in western blot - (reply: 3)
699. Western Blot Data Analysis - (reply: 2)
700. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
701. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
702. Protein Expression in stationary phase - (reply: 11)
703. Protein Smearing - (reply: 2)
704. Western blot for muscle - (reply: 2)
705. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
706. Identify the band in western blot - (reply: 5)
707. What kind of post-translational modification do you think are these? - (reply: 2)
708. Need help with Caspase WB - (reply: 2)
709. to keep sds-pa gels - (reply: 2)
710. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
711. Ponceau staining - Appearence of bands (reply: 1)
712. Detecting phosphorylated Erk1/2 - (reply: 6)
713. Detecting BDNF with WB... - troubleshooting... (reply: 2)
714. western blot using culture media - (reply: 4)
715. Re-inserting marker into scanned WB - (reply: 1)
716. pre stained marker issues - (reply: 2)
717. Functions of BSA and Tween 20 in western blotting - (reply: 2)
718. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
719. Western with conjugated antibody? - (reply: 2)
720. Protein bands on the bottom of the gel - (reply: 5)

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