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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. Western blot of urine samples - Problems verifying loading (reply: 1)
692. BSA test for native PAGE - (reply: 2)
693. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
694. transfer: amperes or volts? - (reply: 6)
695. dot blot works, western doesn't - HELP! - (reply: 5)
696. Liposome + IgG sample in SDS-PAGE - (reply: 1)
697. SDS-PAGE for proteins which have pI>7 - (reply: 1)
698. band diffusion in sds - (reply: 5)
699. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
700. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
701. Artifacts only in Reduced samples!!! - (reply: 18)
702. Over-current alarm - (reply: 1)
703. too much bands in western - (reply: 2)
704. PVDF membrane dried after transfer (Western) - (reply: 6)
705. Help Stained after blocking and won't unstain!!! - (reply: 1)
706. Bands are lower than actual protein - (reply: 1)
707. Protein lysate storage - (reply: 2)
708. Is there alpha-tubulin in the nucleus? - (reply: 4)
709. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
710. No band after WB revelation - (reply: 2)
711. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
712. Expression by ICC but not by Western-why? - (reply: 3)
713. Re-Imaging without Stripping - (reply: 4)
714. MES buffer turned yellow - (reply: 5)
715. western blot woes - (reply: 2)
716. Size larger than expected - totally boggled - (reply: 2)
717. Ponceau Red Staining Problem - (reply: 2)
718. SDS-PAGE Second interface - (reply: 3)
719. biorad's 30% bis/acrylamide storage - (reply: 2)
720. protein concentration per well - (reply: 6)

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