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691. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
692. How to determine final amount of 20 ng protein per WB well - (reply: 8)
693. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
694. nonreducing antibody in reducing condition - (reply: 1)
695. High Molecular weight protein separation - (reply: 2)
696. Secreted protein for loading control - (reply: 2)
697. Only borders stained in Silver!!! - (reply: 7)
698. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
699. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
700. bromophenol blue - (reply: 10)
701. Native Page transfer/detection problems - (reply: 1)
702. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
703. nuclear loading control - (reply: 2)
704. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
705. Large protein transfer problem - (reply: 8)
706. overloading GAPDH but nice band of interest - (reply: 2)
707. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
708. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
709. Quantiying bands. - Determination of protein contens (reply: 2)
710. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
711. Strange lane pattern, yes/no pattern - (reply: 3)
712. Native PAGE w. Western protein marker - (reply: 4)
713. protein stuck - (reply: 3)
714. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
715. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
716. changing colour of front dye in sds-page - (reply: 10)
717. small protein blotting - (reply: 8)
718. how stable is alpha mouse HRP? - left on the bench (reply: 2)
719. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
720. Too many bands in my SDS-PAGE - (reply: 5)
692. How to determine final amount of 20 ng protein per WB well - (reply: 8)
693. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
694. nonreducing antibody in reducing condition - (reply: 1)
695. High Molecular weight protein separation - (reply: 2)
696. Secreted protein for loading control - (reply: 2)
697. Only borders stained in Silver!!! - (reply: 7)
698. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
699. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
700. bromophenol blue - (reply: 10)
701. Native Page transfer/detection problems - (reply: 1)
702. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
703. nuclear loading control - (reply: 2)
704. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
705. Large protein transfer problem - (reply: 8)
706. overloading GAPDH but nice band of interest - (reply: 2)
707. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
708. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
709. Quantiying bands. - Determination of protein contens (reply: 2)
710. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
711. Strange lane pattern, yes/no pattern - (reply: 3)
712. Native PAGE w. Western protein marker - (reply: 4)
713. protein stuck - (reply: 3)
714. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
715. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
716. changing colour of front dye in sds-page - (reply: 10)
717. small protein blotting - (reply: 8)
718. how stable is alpha mouse HRP? - left on the bench (reply: 2)
719. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
720. Too many bands in my SDS-PAGE - (reply: 5)