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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. non-specific bands - (reply: 5)
692. Crazy looking BN-PAGE gel - No, really. (reply: 5)
693. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
694. bands of protein seems like it did not move - (reply: 2)
695. downshifted band - (reply: 4)
696. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
697. Western Troubleshooting Help, please! - (reply: 3)
698. Re-use membrane in Western Blot - (reply: 3)
699. NP40 or Triton as membrane protein detergent - (reply: 1)
700. Dot blot- great, western blot - no signal - (reply: 3)
701. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
702. Problem in transfer high MW protein - (reply: 5)
703. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
704. dimerization or aggregation of my protein! - (reply: 3)
705. basic question in SDS and western blot - (reply: 1)
706. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
707. Transfer problems - (reply: 1)
708. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
709. Stripping temperature - have no 55C water bath (reply: 1)
710. Nonspecificity of anti-flag - (reply: 2)
711. Blocking buffer - (reply: 7)
712. Detection of phospho-proteins - (reply: 1)
713. light chain - (reply: 5)
714. ugly merged bands in western blot - (reply: 3)
715. Western Blot Data Analysis - (reply: 2)
716. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
717. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
718. Protein Expression in stationary phase - (reply: 11)
719. Protein Smearing - (reply: 2)
720. Western blot for muscle - (reply: 2)

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