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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
691. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
692. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
693. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
694. Western blot fail, time and time again - (reply: 21)
695. Western blot of urine samples - Problems verifying loading (reply: 1)
696. BSA test for native PAGE - (reply: 2)
697. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
698. transfer: amperes or volts? - (reply: 6)
699. dot blot works, western doesn't - HELP! - (reply: 5)
700. Liposome + IgG sample in SDS-PAGE - (reply: 1)
701. SDS-PAGE for proteins which have pI>7 - (reply: 1)
702. band diffusion in sds - (reply: 5)
703. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
704. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
705. Artifacts only in Reduced samples!!! - (reply: 18)
706. Over-current alarm - (reply: 1)
707. too much bands in western - (reply: 2)
708. PVDF membrane dried after transfer (Western) - (reply: 6)
709. Help Stained after blocking and won't unstain!!! - (reply: 1)
710. Bands are lower than actual protein - (reply: 1)
711. Protein lysate storage - (reply: 2)
712. Is there alpha-tubulin in the nucleus? - (reply: 4)
713. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
714. No band after WB revelation - (reply: 2)
715. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
716. Expression by ICC but not by Western-why? - (reply: 3)
717. Re-Imaging without Stripping - (reply: 4)
718. MES buffer turned yellow - (reply: 5)
719. western blot woes - (reply: 2)
720. Size larger than expected - totally boggled - (reply: 2)

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