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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. washing after blocking PVDF filter - (reply: 2)
602. western blot - substarate buffer recepie (reply: 2)
603. Saving lysate with laemmli in -20? - (reply: 5)
604. Cell Lysates from a Western Blog - (reply: 2)
605. Dry protein gels without cracking - (reply: 5)
606. power supply electrophoresis - (reply: 11)
607. The Good Antibodies for Western Blots Thread - where people share info on antibodies that work well (reply: 3)
608. Fat bands on 15% SDS-PAGE gel - (reply: 10)
609. how to remove aspecifics on western blot filter - (reply: 2)
610. resolving gel pourcentage - (reply: 3)
611. dye protein ladder - (reply: 5)
612. BAD blots - so many problems they donīt fit in a title (reply: 3)
613. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
614. Trouble-shooting Tris-Tricine SDS-PAGE - fussy dye front (reply: 4)
615. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
616. RIPA Buffer turns Bradford into Blue - (reply: 2)
617. freezing antibody lyophilised - (reply: 2)
618. Nitrocellulose membrane storage after transfer - (reply: 6)
619. western without tween - (reply: 3)
620. Bubbles on the marker after transfer - (reply: 2)
621. Missing patches on immunoblot - please help! - (reply: 7)
622. stupid question - (reply: 8)
623. GAPDH merging lanes - (reply: 1)
624. Band at same position using different primary antibodies - (reply: 3)
625. Western for very small protein - (reply: 3)
626. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
627. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
628. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
629. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
630. Western Blot Secondary Antibody Diluent - basic question (reply: 3)

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