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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. SDS-PAGE with 500 ug protein - (reply: 3)
602. washing after blocking PVDF filter - (reply: 2)
603. western blot - substarate buffer recepie (reply: 2)
604. Saving lysate with laemmli in -20? - (reply: 5)
605. Cell Lysates from a Western Blog - (reply: 2)
606. Dry protein gels without cracking - (reply: 5)
607. power supply electrophoresis - (reply: 11)
608. The Good Antibodies for Western Blots Thread - where people share info on antibodies that work well (reply: 3)
609. Fat bands on 15% SDS-PAGE gel - (reply: 10)
610. how to remove aspecifics on western blot filter - (reply: 2)
611. resolving gel pourcentage - (reply: 3)
612. dye protein ladder - (reply: 5)
613. BAD blots - so many problems they donīt fit in a title (reply: 3)
614. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
615. Trouble-shooting Tris-Tricine SDS-PAGE - fussy dye front (reply: 4)
616. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
617. RIPA Buffer turns Bradford into Blue - (reply: 2)
618. freezing antibody lyophilised - (reply: 2)
619. Nitrocellulose membrane storage after transfer - (reply: 6)
620. western without tween - (reply: 3)
621. Bubbles on the marker after transfer - (reply: 2)
622. Missing patches on immunoblot - please help! - (reply: 7)
623. stupid question - (reply: 8)
624. GAPDH merging lanes - (reply: 1)
625. Band at same position using different primary antibodies - (reply: 3)
626. Western for very small protein - (reply: 3)
627. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
628. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
629. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
630. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)

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