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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. loading buffer and western blot help! - (reply: 5)
602. "Sandy WB" - (reply: 9)
603. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
604. SDS PAGE- HELP - Bands not visible (reply: 4)
605. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
606. Strange SDS gel after Western Blotting - (reply: 1)
607. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
608. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
609. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
610. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
611. Western blot fail, time and time again - (reply: 21)
612. Western blot of urine samples - Problems verifying loading (reply: 1)
613. BSA test for native PAGE - (reply: 2)
614. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
615. transfer: amperes or volts? - (reply: 6)
616. dot blot works, western doesn't - HELP! - (reply: 5)
617. Liposome + IgG sample in SDS-PAGE - (reply: 1)
618. SDS-PAGE for proteins which have pI>7 - (reply: 1)
619. band diffusion in sds - (reply: 5)
620. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
621. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
622. Artifacts only in Reduced samples!!! - (reply: 18)
623. Over-current alarm - (reply: 1)
624. too much bands in western - (reply: 2)
625. PVDF membrane dried after transfer (Western) - (reply: 6)
626. Help Stained after blocking and won't unstain!!! - (reply: 1)
627. Bands are lower than actual protein - (reply: 1)
628. Protein lysate storage - (reply: 2)
629. Is there alpha-tubulin in the nucleus? - (reply: 4)
630. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)

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