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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. Is there alpha-tubulin in the nucleus? - (reply: 4)
602. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
603. No band after WB revelation - (reply: 2)
604. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
605. Expression by ICC but not by Western-why? - (reply: 3)
606. Re-Imaging without Stripping - (reply: 4)
607. MES buffer turned yellow - (reply: 5)
608. western blot woes - (reply: 2)
609. Size larger than expected - totally boggled - (reply: 2)
610. Ponceau Red Staining Problem - (reply: 2)
611. SDS-PAGE Second interface - (reply: 3)
612. biorad's 30% bis/acrylamide storage - (reply: 2)
613. protein concentration per well - (reply: 6)
614. dot blot - Problem (reply: 1)
615. Clear native PAGE - (reply: 1)
616. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
617. Weak western signal for help - (reply: 13)
618. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
619. Western not working anymore - (reply: 15)
620. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
621. BN-PAGE possible with Tris-glycine - (reply: 1)
622. Western few questions - (reply: 1)
623. thiourea in 2D page - What's it for? (reply: 1)
624. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
625. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
626. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
627. Antibody works in immunofluorescence, but not in WB - (reply: 4)
628. B-actin as western blot -control - (reply: 4)
629. horrific western blots , please help - (reply: 3)
630. Trouble with actin, HA and CCND1 :(( - (reply: 1)

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