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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. Liposome + IgG sample in SDS-PAGE - (reply: 1)
602. SDS-PAGE for proteins which have pI>7 - (reply: 1)
603. band diffusion in sds - (reply: 5)
604. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
605. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
606. Artifacts only in Reduced samples!!! - (reply: 18)
607. Over-current alarm - (reply: 1)
608. too much bands in western - (reply: 2)
609. PVDF membrane dried after transfer (Western) - (reply: 6)
610. Help Stained after blocking and won't unstain!!! - (reply: 1)
611. Bands are lower than actual protein - (reply: 1)
612. Protein lysate storage - (reply: 2)
613. Is there alpha-tubulin in the nucleus? - (reply: 4)
614. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
615. No band after WB revelation - (reply: 2)
616. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
617. Expression by ICC but not by Western-why? - (reply: 3)
618. Re-Imaging without Stripping - (reply: 4)
619. MES buffer turned yellow - (reply: 5)
620. western blot woes - (reply: 2)
621. Size larger than expected - totally boggled - (reply: 2)
622. Ponceau Red Staining Problem - (reply: 2)
623. SDS-PAGE Second interface - (reply: 3)
624. biorad's 30% bis/acrylamide storage - (reply: 2)
625. protein concentration per well - (reply: 6)
626. dot blot - Problem (reply: 1)
627. Clear native PAGE - (reply: 1)
628. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
629. Weak western signal for help - (reply: 13)
630. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)

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