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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. resolving gel pourcentage - (reply: 3)
602. dye protein ladder - (reply: 5)
603. BAD blots - so many problems they donīt fit in a title (reply: 3)
604. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
605. Trouble-shooting Tris-Tricine SDS-PAGE - fussy dye front (reply: 4)
606. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
607. RIPA Buffer turns Bradford into Blue - (reply: 2)
608. freezing antibody lyophilised - (reply: 2)
609. Nitrocellulose membrane storage after transfer - (reply: 6)
610. western without tween - (reply: 3)
611. Bubbles on the marker after transfer - (reply: 2)
612. Missing patches on immunoblot - please help! - (reply: 7)
613. stupid question - (reply: 8)
614. GAPDH merging lanes - (reply: 1)
615. Band at same position using different primary antibodies - (reply: 3)
616. Western for very small protein - (reply: 3)
617. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
618. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
619. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
620. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
621. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
622. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
623. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
624. What cause "downshift" in SDS-PAGE? - (reply: 5)
625. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
626. Blue native PAGE problems - (reply: 1)
627. running problems SDS-PAGE gel (zymography) - (reply: 4)
628. Western unresolved lanes - (reply: 2)
629. Doing WB from scratch for the first time. Need some info. - (reply: 2)
630. Western blot bovine collagen typ I problem - (reply: 3)

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