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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. freezing antibody lyophilised - (reply: 2)
602. Nitrocellulose membrane storage after transfer - (reply: 6)
603. western without tween - (reply: 3)
604. Bubbles on the marker after transfer - (reply: 2)
605. Missing patches on immunoblot - please help! - (reply: 7)
606. stupid question - (reply: 8)
607. GAPDH merging lanes - (reply: 1)
608. Band at same position using different primary antibodies - (reply: 3)
609. Western for very small protein - (reply: 3)
610. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
611. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
612. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
613. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
614. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
615. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
616. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
617. What cause "downshift" in SDS-PAGE? - (reply: 5)
618. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
619. Blue native PAGE problems - (reply: 1)
620. running problems SDS-PAGE gel (zymography) - (reply: 4)
621. Western unresolved lanes - (reply: 2)
622. Doing WB from scratch for the first time. Need some info. - (reply: 2)
623. Western blot bovine collagen typ I problem - (reply: 3)
624. no band, extremely clean blot - (reply: 1)
625. Acrylamide stock - (reply: 2)
626. low signal in WB - (reply: 3)
627. standard internal marker for quantification - (reply: 3)
628. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
629. IgG - unspecific band (reply: 3)
630. red ponceau - (reply: 3)

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