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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
602. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
603. Western blot fail, time and time again - (reply: 21)
604. Western blot of urine samples - Problems verifying loading (reply: 1)
605. BSA test for native PAGE - (reply: 2)
606. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
607. transfer: amperes or volts? - (reply: 6)
608. dot blot works, western doesn't - HELP! - (reply: 5)
609. Liposome + IgG sample in SDS-PAGE - (reply: 1)
610. SDS-PAGE for proteins which have pI>7 - (reply: 1)
611. band diffusion in sds - (reply: 5)
612. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
613. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
614. Artifacts only in Reduced samples!!! - (reply: 18)
615. Over-current alarm - (reply: 1)
616. too much bands in western - (reply: 2)
617. PVDF membrane dried after transfer (Western) - (reply: 6)
618. Help Stained after blocking and won't unstain!!! - (reply: 1)
619. Bands are lower than actual protein - (reply: 1)
620. Protein lysate storage - (reply: 2)
621. Is there alpha-tubulin in the nucleus? - (reply: 4)
622. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
623. No band after WB revelation - (reply: 2)
624. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
625. Expression by ICC but not by Western-why? - (reply: 3)
626. Re-Imaging without Stripping - (reply: 4)
627. MES buffer turned yellow - (reply: 5)
628. western blot woes - (reply: 2)
629. Size larger than expected - totally boggled - (reply: 2)
630. Ponceau Red Staining Problem - (reply: 2)

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