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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
602. RIPA Buffer turns Bradford into Blue - (reply: 2)
603. freezing antibody lyophilised - (reply: 2)
604. Nitrocellulose membrane storage after transfer - (reply: 6)
605. western without tween - (reply: 3)
606. Bubbles on the marker after transfer - (reply: 2)
607. Missing patches on immunoblot - please help! - (reply: 7)
608. stupid question - (reply: 8)
609. GAPDH merging lanes - (reply: 1)
610. Band at same position using different primary antibodies - (reply: 3)
611. Western for very small protein - (reply: 3)
612. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
613. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
614. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
615. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
616. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
617. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
618. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
619. What cause "downshift" in SDS-PAGE? - (reply: 5)
620. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
621. Blue native PAGE problems - (reply: 1)
622. running problems SDS-PAGE gel (zymography) - (reply: 4)
623. Western unresolved lanes - (reply: 2)
624. Doing WB from scratch for the first time. Need some info. - (reply: 2)
625. Western blot bovine collagen typ I problem - (reply: 3)
626. no band, extremely clean blot - (reply: 1)
627. Acrylamide stock - (reply: 2)
628. low signal in WB - (reply: 3)
629. standard internal marker for quantification - (reply: 3)
630. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)

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