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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
602. Trouble-shooting Tris-Tricine SDS-PAGE - fussy dye front (reply: 4)
603. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
604. RIPA Buffer turns Bradford into Blue - (reply: 2)
605. freezing antibody lyophilised - (reply: 2)
606. Nitrocellulose membrane storage after transfer - (reply: 6)
607. western without tween - (reply: 3)
608. Bubbles on the marker after transfer - (reply: 2)
609. Missing patches on immunoblot - please help! - (reply: 7)
610. stupid question - (reply: 8)
611. GAPDH merging lanes - (reply: 1)
612. Band at same position using different primary antibodies - (reply: 3)
613. Western for very small protein - (reply: 3)
614. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
615. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
616. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
617. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
618. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
619. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
620. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
621. What cause "downshift" in SDS-PAGE? - (reply: 5)
622. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
623. Blue native PAGE problems - (reply: 1)
624. running problems SDS-PAGE gel (zymography) - (reply: 4)
625. Western unresolved lanes - (reply: 2)
626. Doing WB from scratch for the first time. Need some info. - (reply: 2)
627. Western blot bovine collagen typ I problem - (reply: 3)
628. no band, extremely clean blot - (reply: 1)
629. Acrylamide stock - (reply: 2)
630. low signal in WB - (reply: 3)

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