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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. SDS-PAGE Second interface - (reply: 3)
602. biorad's 30% bis/acrylamide storage - (reply: 2)
603. protein concentration per well - (reply: 6)
604. dot blot - Problem (reply: 1)
605. Clear native PAGE - (reply: 1)
606. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
607. Weak western signal for help - (reply: 13)
608. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
609. Western not working anymore - (reply: 15)
610. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
611. BN-PAGE possible with Tris-glycine - (reply: 1)
612. Western few questions - (reply: 1)
613. thiourea in 2D page - What's it for? (reply: 1)
614. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
615. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
616. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
617. Antibody works in immunofluorescence, but not in WB - (reply: 4)
618. B-actin as western blot -control - (reply: 4)
619. horrific western blots , please help - (reply: 3)
620. Trouble with actin, HA and CCND1 :(( - (reply: 1)
621. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
622. Lower MW bands than expected. - (reply: 1)
623. Question about reducing agent (DTT) - (reply: 1)
624. Collagen II western blotting - (reply: 1)
625. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
626. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
627. NUCLEAR PROTEIN PREPARATION - (reply: 1)
628. Lost protein from blot? - (reply: 1)
629. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
630. No SDS in gels - (reply: 12)

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