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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
601. how to remove aspecifics on western blot filter - (reply: 2)
602. resolving gel pourcentage - (reply: 3)
603. dye protein ladder - (reply: 5)
604. BAD blots - so many problems they donīt fit in a title (reply: 3)
605. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
606. Trouble-shooting Tris-Tricine SDS-PAGE - fussy dye front (reply: 4)
607. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
608. RIPA Buffer turns Bradford into Blue - (reply: 2)
609. freezing antibody lyophilised - (reply: 2)
610. Nitrocellulose membrane storage after transfer - (reply: 6)
611. western without tween - (reply: 3)
612. Bubbles on the marker after transfer - (reply: 2)
613. Missing patches on immunoblot - please help! - (reply: 7)
614. stupid question - (reply: 8)
615. GAPDH merging lanes - (reply: 1)
616. Band at same position using different primary antibodies - (reply: 3)
617. Western for very small protein - (reply: 3)
618. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
619. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
620. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
621. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
622. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
623. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
624. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
625. What cause "downshift" in SDS-PAGE? - (reply: 5)
626. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
627. Blue native PAGE problems - (reply: 1)
628. running problems SDS-PAGE gel (zymography) - (reply: 4)
629. Western unresolved lanes - (reply: 2)
630. Doing WB from scratch for the first time. Need some info. - (reply: 2)

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