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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
571. Longer incubation in primary antibody - (reply: 2)
572. Cell Lysis Buffer - (reply: 6)
573. Strange Western blot background - (reply: 2)
574. question about denaturing - (reply: 1)
575. Secondary antibody recovery - (reply: 4)
576. Help! I left my blot in primary at room temp overnight - (reply: 1)
577. Help! I left my blot in primary at room temp overnight - (reply: 1)
578. Help with Twist antibody? - (reply: 1)
579. Denaturing protein before blotting - (reply: 1)
580. Lysis buffer selection - (reply: 2)
581. not completed transfer proteins on NT membrane - western blot (reply: 2)
582. loading buffer and western blot help! - (reply: 5)
583. "Sandy WB" - (reply: 9)
584. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
585. SDS PAGE- HELP - Bands not visible (reply: 4)
586. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
587. Strange SDS gel after Western Blotting - (reply: 1)
588. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
589. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
590. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
591. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
592. Western blot fail, time and time again - (reply: 21)
593. Western blot of urine samples - Problems verifying loading (reply: 1)
594. BSA test for native PAGE - (reply: 2)
595. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
596. transfer: amperes or volts? - (reply: 6)
597. dot blot works, western doesn't - HELP! - (reply: 5)
598. Liposome + IgG sample in SDS-PAGE - (reply: 1)
599. SDS-PAGE for proteins which have pI>7 - (reply: 1)
600. band diffusion in sds - (reply: 5)

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