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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
571. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
572. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
573. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
574. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
575. Western blot fail, time and time again - (reply: 21)
576. Western blot of urine samples - Problems verifying loading (reply: 1)
577. BSA test for native PAGE - (reply: 2)
578. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
579. transfer: amperes or volts? - (reply: 6)
580. dot blot works, western doesn't - HELP! - (reply: 5)
581. Liposome + IgG sample in SDS-PAGE - (reply: 1)
582. SDS-PAGE for proteins which have pI>7 - (reply: 1)
583. band diffusion in sds - (reply: 5)
584. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
585. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
586. Artifacts only in Reduced samples!!! - (reply: 18)
587. Over-current alarm - (reply: 1)
588. too much bands in western - (reply: 2)
589. PVDF membrane dried after transfer (Western) - (reply: 6)
590. Help Stained after blocking and won't unstain!!! - (reply: 1)
591. Bands are lower than actual protein - (reply: 1)
592. Protein lysate storage - (reply: 2)
593. Is there alpha-tubulin in the nucleus? - (reply: 4)
594. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
595. No band after WB revelation - (reply: 2)
596. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
597. Expression by ICC but not by Western-why? - (reply: 3)
598. Re-Imaging without Stripping - (reply: 4)
599. MES buffer turned yellow - (reply: 5)
600. western blot woes - (reply: 2)

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