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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
571. What cause "downshift" in SDS-PAGE? - (reply: 5)
572. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
573. Blue native PAGE problems - (reply: 1)
574. running problems SDS-PAGE gel (zymography) - (reply: 4)
575. Western unresolved lanes - (reply: 2)
576. Doing WB from scratch for the first time. Need some info. - (reply: 2)
577. Western blot bovine collagen typ I problem - (reply: 3)
578. no band, extremely clean blot - (reply: 1)
579. Acrylamide stock - (reply: 2)
580. low signal in WB - (reply: 3)
581. standard internal marker for quantification - (reply: 3)
582. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
583. IgG - unspecific band (reply: 3)
584. red ponceau - (reply: 3)
585. Noisy fluorescent gels - (reply: 1)
586. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
587. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
588. resolving and stucking gel solutions - (reply: 2)
589. bradford absorbance - (reply: 1)
590. protein degradation - (reply: 3)
591. Dry or not PVDF membrane after transfer - (reply: 2)
592. I see bands with western blot only the third day! - (reply: 2)
593. Western Blots on Membrane Proteins - (reply: 9)
594. Help- voltage going sky high- don't know why - (reply: 1)
595. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
596. efficient stripping buffer - (reply: 2)
597. quantification of a protein in tissue - (reply: 3)
598. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
599. Help - proteins faster than the dye front - (reply: 8)
600. Longer incubation in primary antibody - (reply: 2)

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