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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
571. SDS-PAGE for proteins which have pI>7 - (reply: 1)
572. band diffusion in sds - (reply: 5)
573. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
574. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
575. Artifacts only in Reduced samples!!! - (reply: 18)
576. Over-current alarm - (reply: 1)
577. too much bands in western - (reply: 2)
578. PVDF membrane dried after transfer (Western) - (reply: 6)
579. Help Stained after blocking and won't unstain!!! - (reply: 1)
580. Bands are lower than actual protein - (reply: 1)
581. Protein lysate storage - (reply: 2)
582. Is there alpha-tubulin in the nucleus? - (reply: 4)
583. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
584. No band after WB revelation - (reply: 2)
585. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
586. Expression by ICC but not by Western-why? - (reply: 3)
587. Re-Imaging without Stripping - (reply: 4)
588. MES buffer turned yellow - (reply: 5)
589. western blot woes - (reply: 2)
590. Size larger than expected - totally boggled - (reply: 2)
591. Ponceau Red Staining Problem - (reply: 2)
592. SDS-PAGE Second interface - (reply: 3)
593. biorad's 30% bis/acrylamide storage - (reply: 2)
594. protein concentration per well - (reply: 6)
595. dot blot - Problem (reply: 1)
596. Clear native PAGE - (reply: 1)
597. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
598. Weak western signal for help - (reply: 13)
599. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
600. Western not working anymore - (reply: 15)

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