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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
571. quantification of a protein in tissue - (reply: 3)
572. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
573. Help - proteins faster than the dye front - (reply: 8)
574. Longer incubation in primary antibody - (reply: 2)
575. Cell Lysis Buffer - (reply: 6)
576. Strange Western blot background - (reply: 2)
577. question about denaturing - (reply: 1)
578. Secondary antibody recovery - (reply: 4)
579. Help! I left my blot in primary at room temp overnight - (reply: 1)
580. Help! I left my blot in primary at room temp overnight - (reply: 1)
581. Help with Twist antibody? - (reply: 1)
582. Denaturing protein before blotting - (reply: 1)
583. Lysis buffer selection - (reply: 2)
584. not completed transfer proteins on NT membrane - western blot (reply: 2)
585. loading buffer and western blot help! - (reply: 5)
586. "Sandy WB" - (reply: 9)
587. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
588. SDS PAGE- HELP - Bands not visible (reply: 4)
589. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
590. Strange SDS gel after Western Blotting - (reply: 1)
591. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
592. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
593. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
594. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
595. Western blot fail, time and time again - (reply: 21)
596. Western blot of urine samples - Problems verifying loading (reply: 1)
597. BSA test for native PAGE - (reply: 2)
598. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
599. transfer: amperes or volts? - (reply: 6)
600. dot blot works, western doesn't - HELP! - (reply: 5)

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