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571. NP40 or Triton as membrane protein detergent - (reply: 1)
572. Dot blot- great, western blot - no signal - (reply: 3)
573. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
574. Problem in transfer high MW protein - (reply: 5)
575. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
576. dimerization or aggregation of my protein! - (reply: 3)
577. basic question in SDS and western blot - (reply: 1)
578. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
579. Transfer problems - (reply: 1)
580. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
581. Stripping temperature - have no 55C water bath (reply: 1)
582. Nonspecificity of anti-flag - (reply: 2)
583. Blocking buffer - (reply: 7)
584. Detection of phospho-proteins - (reply: 1)
585. light chain - (reply: 5)
586. ugly merged bands in western blot - (reply: 3)
587. Western Blot Data Analysis - (reply: 2)
588. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
589. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
590. Protein Expression in stationary phase - (reply: 11)
591. Protein Smearing - (reply: 2)
592. Western blot for muscle - (reply: 2)
593. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
594. Identify the band in western blot - (reply: 5)
595. What kind of post-translational modification do you think are these？ - (reply: 2)
596. Need help with Caspase WB - (reply: 2)
597. to keep sds-pa gels - (reply: 2)
598. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
599. Ponceau staining - Appearence of bands (reply: 1)
600. Detecting phosphorylated Erk1/2 - (reply: 6)
572. Dot blot- great, western blot - no signal - (reply: 3)
573. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
574. Problem in transfer high MW protein - (reply: 5)
575. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
576. dimerization or aggregation of my protein! - (reply: 3)
577. basic question in SDS and western blot - (reply: 1)
578. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
579. Transfer problems - (reply: 1)
580. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
581. Stripping temperature - have no 55C water bath (reply: 1)
582. Nonspecificity of anti-flag - (reply: 2)
583. Blocking buffer - (reply: 7)
584. Detection of phospho-proteins - (reply: 1)
585. light chain - (reply: 5)
586. ugly merged bands in western blot - (reply: 3)
587. Western Blot Data Analysis - (reply: 2)
588. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
589. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
590. Protein Expression in stationary phase - (reply: 11)
591. Protein Smearing - (reply: 2)
592. Western blot for muscle - (reply: 2)
593. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
594. Identify the band in western blot - (reply: 5)
595. What kind of post-translational modification do you think are these？ - (reply: 2)
596. Need help with Caspase WB - (reply: 2)
597. to keep sds-pa gels - (reply: 2)
598. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
599. Ponceau staining - Appearence of bands (reply: 1)
600. Detecting phosphorylated Erk1/2 - (reply: 6)