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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
571. Lysis buffer selection - (reply: 2)
572. not completed transfer proteins on NT membrane - western blot (reply: 2)
573. loading buffer and western blot help! - (reply: 5)
574. "Sandy WB" - (reply: 9)
575. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
576. SDS PAGE- HELP - Bands not visible (reply: 4)
577. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
578. Strange SDS gel after Western Blotting - (reply: 1)
579. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
580. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
581. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
582. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
583. Western blot fail, time and time again - (reply: 21)
584. Western blot of urine samples - Problems verifying loading (reply: 1)
585. BSA test for native PAGE - (reply: 2)
586. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
587. transfer: amperes or volts? - (reply: 6)
588. dot blot works, western doesn't - HELP! - (reply: 5)
589. Liposome + IgG sample in SDS-PAGE - (reply: 1)
590. SDS-PAGE for proteins which have pI>7 - (reply: 1)
591. band diffusion in sds - (reply: 5)
592. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
593. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
594. Artifacts only in Reduced samples!!! - (reply: 18)
595. Over-current alarm - (reply: 1)
596. too much bands in western - (reply: 2)
597. PVDF membrane dried after transfer (Western) - (reply: 6)
598. Help Stained after blocking and won't unstain!!! - (reply: 1)
599. Bands are lower than actual protein - (reply: 1)
600. Protein lysate storage - (reply: 2)

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