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481. Protein lysate storage - (reply: 2)
482. Is there alpha-tubulin in the nucleus? - (reply: 4)
483. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
484. No band after WB revelation - (reply: 2)
485. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
486. Expression by ICC but not by Western-why? - (reply: 3)
487. Re-Imaging without Stripping - (reply: 4)
488. MES buffer turned yellow - (reply: 5)
489. western blot woes - (reply: 2)
490. Size larger than expected - totally boggled - (reply: 2)
491. Ponceau Red Staining Problem - (reply: 2)
492. SDS-PAGE Second interface - (reply: 3)
493. biorad's 30% bis/acrylamide storage - (reply: 2)
494. protein concentration per well - (reply: 6)
495. dot blot - Problem (reply: 1)
496. Clear native PAGE - (reply: 1)
497. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
498. Weak western signal for help - (reply: 13)
499. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
500. Western not working anymore - (reply: 15)
501. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
502. BN-PAGE possible with Tris-glycine - (reply: 1)
503. Western few questions - (reply: 1)
504. thiourea in 2D page - What's it for? (reply: 1)
505. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
506. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
507. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
508. Antibody works in immunofluorescence, but not in WB - (reply: 4)
509. B-actin as western blot -control - (reply: 4)
510. horrific western blots , please help - (reply: 3)
482. Is there alpha-tubulin in the nucleus? - (reply: 4)
483. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
484. No band after WB revelation - (reply: 2)
485. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
486. Expression by ICC but not by Western-why? - (reply: 3)
487. Re-Imaging without Stripping - (reply: 4)
488. MES buffer turned yellow - (reply: 5)
489. western blot woes - (reply: 2)
490. Size larger than expected - totally boggled - (reply: 2)
491. Ponceau Red Staining Problem - (reply: 2)
492. SDS-PAGE Second interface - (reply: 3)
493. biorad's 30% bis/acrylamide storage - (reply: 2)
494. protein concentration per well - (reply: 6)
495. dot blot - Problem (reply: 1)
496. Clear native PAGE - (reply: 1)
497. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
498. Weak western signal for help - (reply: 13)
499. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
500. Western not working anymore - (reply: 15)
501. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
502. BN-PAGE possible with Tris-glycine - (reply: 1)
503. Western few questions - (reply: 1)
504. thiourea in 2D page - What's it for? (reply: 1)
505. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
506. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
507. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
508. Antibody works in immunofluorescence, but not in WB - (reply: 4)
509. B-actin as western blot -control - (reply: 4)
510. horrific western blots , please help - (reply: 3)