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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. ECL Prime Streamlining and Troubleshootin - (reply: 1)
482. Samples leaking out of wells in gel - (reply: 2)
483. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
484. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
485. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
486. Strange E.coli growth - (reply: 3)
487. Problem with Immunoprecipitated samples - (reply: 2)
488. problems in transfer - (reply: 3)
489. Phospho-JNK - (reply: 9)
490. Bradford reagent for staining gels? - (reply: 1)
491. developing film manually - (reply: 19)
492. non specific bands - (reply: 7)
493. BSA vs Milk - (reply: 2)
494. Western Blot no target bands, show Non-specific bands - (reply: 5)
495. how critical is the pH of tbst? - (reply: 2)
496. Fc fusion protein smearing on SDS PAGE - (reply: 2)
497. what do you recommend for Semi-Dry unit - (reply: 2)
498. Bands disappear after longer exposure - (reply: 4)
499. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
500. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
501. Antibody Optimization - (reply: 2)
502. Protein agregation in WB samples - (reply: 3)
503. Same Non-specific bands show up for different Abs!! - (reply: 7)
504. what caused bubble band of my WB? - (reply: 4)
505. blotting detection - (reply: 3)
506. Max amount of protein - (reply: 4)
507. Areas of membrane bleached - (reply: 1)
508. asking suggestions for western blot - (reply: 3)
509. Can i apply primary antibody for two nights instead of one? - (reply: 3)
510. Strange bands and large smear in western blot - (reply: 4)

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