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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
482. horizontal smear? - (reply: 2)
483. BSA versus milk in blocking and antibody incubation - (reply: 5)
484. Western blots are 'semi' quantitative? - (reply: 12)
485. bands fuse together - (reply: 1)
486. Phospho-protein detection - (reply: 2)
487. Help on using different antibodies in the same membrane - (reply: 8)
488. weird query for Western Blotting - (reply: 2)
489. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
490. Quick/One-step/automated Western Blot - (reply: 8)
491. Amount of antibody used in Western Blot - (reply: 1)
492. WB Transfer: Voltage vs. Current - (reply: 7)
493. Large protein blot ~400kDa - (reply: 3)
494. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
495. Stripping buffer for nitrocellulose membrane - (reply: 1)
496. PAGE for Separation of DNA - (reply: 3)
497. Western Blotting of Proteins less than 10 kDa - (reply: 1)
498. dot blot - (reply: 7)
499. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
500. Internal Standard for western blot - (reply: 1)
501. Sox-2 antibody detection using Western - (reply: 10)
502. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
503. gH2A.X blotting problem - (reply: 1)
504. Re-using membranes with ghost bands - (reply: 2)
505. large protein - (reply: 3)
506. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
507. problem with loading the protein on to SDS-Gel - (reply: 1)
508. Western blot not showing protein - (reply: 2)
509. normalisation of western blot from kodak film - (reply: 1)
510. antibody in 40% glycerol - (reply: 6)

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