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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
482. Antibody Optimization - (reply: 2)
483. Protein agregation in WB samples - (reply: 3)
484. Same Non-specific bands show up for different Abs!! - (reply: 7)
485. what caused bubble band of my WB? - (reply: 4)
486. blotting detection - (reply: 3)
487. Max amount of protein - (reply: 4)
488. Areas of membrane bleached - (reply: 1)
489. asking suggestions for western blot - (reply: 3)
490. Can i apply primary antibody for two nights instead of one? - (reply: 3)
491. Strange bands and large smear in western blot - (reply: 4)
492. Freezing proteins at -80 - (reply: 1)
493. Tris glycine SDS buffer prep pH error - (reply: 2)
494. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
495. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
496. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
497. problems with negative control - (reply: 2)
498. urgent-W.B visualisation on kodak film - (reply: 2)
499. NP 40 lysis buffer - (reply: 1)
500. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
501. Dark western blot film - (reply: 12)
502. how to get red of high secondary signal - (reply: 4)
503. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
504. Detecting phosphoproteins - different results when samples rerun (reply: 1)
505. Is loading buffer ok? - (reply: 1)
506. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
507. Antibody vs Antigen concentration effect - (reply: 3)
508. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
509. SDS PAGE smearing of bands - (reply: 2)
510. Fuzzy bands in western blot - (reply: 2)

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