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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. Re-using membranes with ghost bands - (reply: 2)
482. large protein - (reply: 3)
483. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
484. problem with loading the protein on to SDS-Gel - (reply: 1)
485. Western blot not showing protein - (reply: 2)
486. normalisation of western blot from kodak film - (reply: 1)
487. antibody in 40% glycerol - (reply: 6)
488. longer running of proteins in SDS PAGE! - (reply: 2)
489. dirty back ground after exposure - (reply: 2)
490. Tenfold PBS in extraction - (reply: 3)
491. Separating close bands in Western Blot - (reply: 3)
492. Western blot on intestine samples - (reply: 2)
493. dot blot - (reply: 4)
494. ECL Prime Streamlining and Troubleshootin - (reply: 1)
495. Samples leaking out of wells in gel - (reply: 2)
496. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
497. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
498. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
499. Strange E.coli growth - (reply: 3)
500. Problem with Immunoprecipitated samples - (reply: 2)
501. problems in transfer - (reply: 3)
502. Phospho-JNK - (reply: 9)
503. Bradford reagent for staining gels? - (reply: 1)
504. developing film manually - (reply: 19)
505. non specific bands - (reply: 7)
506. BSA vs Milk - (reply: 2)
507. Western Blot no target bands, show Non-specific bands - (reply: 5)
508. how critical is the pH of tbst? - (reply: 2)
509. Fc fusion protein smearing on SDS PAGE - (reply: 2)
510. what do you recommend for Semi-Dry unit - (reply: 2)

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