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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. Huge background with HT22 cells phophoERK antibody - (reply: 3)
482. How to quantify the protein amount for serum samples ? - (reply: 2)
483. Huge background with HT22 cells phophoERK antibody - (reply: 1)
484. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
485. horizontal smear? - (reply: 2)
486. BSA versus milk in blocking and antibody incubation - (reply: 5)
487. Western blots are 'semi' quantitative? - (reply: 12)
488. bands fuse together - (reply: 1)
489. Phospho-protein detection - (reply: 2)
490. Help on using different antibodies in the same membrane - (reply: 8)
491. weird query for Western Blotting - (reply: 2)
492. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
493. Quick/One-step/automated Western Blot - (reply: 8)
494. Amount of antibody used in Western Blot - (reply: 1)
495. WB Transfer: Voltage vs. Current - (reply: 7)
496. Large protein blot ~400kDa - (reply: 3)
497. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
498. Stripping buffer for nitrocellulose membrane - (reply: 1)
499. PAGE for Separation of DNA - (reply: 3)
500. Western Blotting of Proteins less than 10 kDa - (reply: 1)
501. dot blot - (reply: 7)
502. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
503. Internal Standard for western blot - (reply: 1)
504. Sox-2 antibody detection using Western - (reply: 10)
505. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
506. gH2A.X blotting problem - (reply: 1)
507. Re-using membranes with ghost bands - (reply: 2)
508. large protein - (reply: 3)
509. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
510. problem with loading the protein on to SDS-Gel - (reply: 1)

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