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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
482. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
483. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
484. problems with negative control - (reply: 2)
485. urgent-W.B visualisation on kodak film - (reply: 2)
486. NP 40 lysis buffer - (reply: 1)
487. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
488. Dark western blot film - (reply: 12)
489. how to get red of high secondary signal - (reply: 4)
490. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
491. Detecting phosphoproteins - different results when samples rerun (reply: 1)
492. Is loading buffer ok? - (reply: 1)
493. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
494. Antibody vs Antigen concentration effect - (reply: 3)
495. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
496. SDS PAGE smearing of bands - (reply: 2)
497. Fuzzy bands in western blot - (reply: 2)
498. I am desperated with my WB!! - (reply: 2)
499. storing SDS-PAGE gels for W.Blot - (reply: 7)
500. trouble detecting phospho-proteins - (reply: 2)
501. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
502. BSA + DTT versus -DTT - (reply: 1)
503. WB - Transferring REALLY big proteins - (reply: 2)
504. Over-stripping blot? - (reply: 5)
505. Oscillation on my Western Blot results - (reply: 3)
506. HELP!!! Non-specific binding on my membrane - (reply: 7)
507. Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)
508. Many unspecific bands after using anti-His on Western blot - (reply: 4)
509. Need help with pre run of PAGE. - (reply: 1)
510. KO mice show band for knocked-out protein - (reply: 1)

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