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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. Max amount of protein - (reply: 4)
482. Areas of membrane bleached - (reply: 1)
483. asking suggestions for western blot - (reply: 3)
484. Can i apply primary antibody for two nights instead of one? - (reply: 3)
485. Strange bands and large smear in western blot - (reply: 4)
486. Freezing proteins at -80 - (reply: 1)
487. Tris glycine SDS buffer prep pH error - (reply: 2)
488. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
489. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
490. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
491. problems with negative control - (reply: 2)
492. urgent-W.B visualisation on kodak film - (reply: 2)
493. NP 40 lysis buffer - (reply: 1)
494. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
495. Dark western blot film - (reply: 12)
496. how to get red of high secondary signal - (reply: 4)
497. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
498. Detecting phosphoproteins - different results when samples rerun (reply: 1)
499. Is loading buffer ok? - (reply: 1)
500. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
501. Antibody vs Antigen concentration effect - (reply: 3)
502. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
503. SDS PAGE smearing of bands - (reply: 2)
504. Fuzzy bands in western blot - (reply: 2)
505. I am desperated with my WB!! - (reply: 2)
506. storing SDS-PAGE gels for W.Blot - (reply: 7)
507. trouble detecting phospho-proteins - (reply: 2)
508. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
509. BSA + DTT versus -DTT - (reply: 1)
510. WB - Transferring REALLY big proteins - (reply: 2)

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