Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
481. BSA concentration for blocking - (reply: 5)
482. Identically loaded western bands loose intensity from left to right - (reply: 3)
483. eIF4G western blot - (reply: 3)
484. smear bands in western blot - (reply: 12)
485. Huge background with HT22 cells phophoERK antibody - (reply: 3)
486. How to quantify the protein amount for serum samples ? - (reply: 2)
487. Huge background with HT22 cells phophoERK antibody - (reply: 1)
488. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
489. horizontal smear? - (reply: 2)
490. BSA versus milk in blocking and antibody incubation - (reply: 5)
491. Western blots are 'semi' quantitative? - (reply: 12)
492. bands fuse together - (reply: 1)
493. Phospho-protein detection - (reply: 2)
494. Help on using different antibodies in the same membrane - (reply: 8)
495. weird query for Western Blotting - (reply: 2)
496. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
497. Quick/One-step/automated Western Blot - (reply: 8)
498. Amount of antibody used in Western Blot - (reply: 1)
499. WB Transfer: Voltage vs. Current - (reply: 7)
500. Large protein blot ~400kDa - (reply: 3)
501. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
502. Stripping buffer for nitrocellulose membrane - (reply: 1)
503. PAGE for Separation of DNA - (reply: 3)
504. Western Blotting of Proteins less than 10 kDa - (reply: 1)
505. dot blot - (reply: 7)
506. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
507. Internal Standard for western blot - (reply: 1)
508. Sox-2 antibody detection using Western - (reply: 10)
509. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
510. gH2A.X blotting problem - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37