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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1021. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
1022. taking protein out of membrane - (reply: 1)
1023. prearing gel in advance - (reply: 6)
1024. freezing after reducing and boiling - (reply: 10)
1025. Invitrogen iBlot - (reply: 6)
1026. Even volumes of different samples - (reply: 3)
1027. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
1028. A single band on WB smaller than expected - (reply: 3)
1029. Ponceau staining after primary and secondary? - (reply: 5)
1030. caspase3 detection - help (reply: 5)
1031. Big white spots on PVDF membrane - (reply: 1)
1032. High background.... - (reply: 6)
1033. WB does not correspond to protein array - (reply: 1)
1034. Strange pattern after transfer - (reply: 8)
1035. Quantifying Western blots - (reply: 1)
1036. Complete transfer, can it be done or not? - (reply: 4)
1037. Can add new secondary Ab? - (reply: 1)
1038. Dot blot - (reply: 2)
1039. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
1040. anti-phosphotyrosine blot on 2mo old samples? - (reply: 1)
1041. NATIVE PAGE - (reply: 1)
1042. Looking for new endogenous control for WB - (reply: 3)
1043. wash blots between antibody incubations with TB-'SALINE' pH 7.4 - why saline.. why this pH? (reply: 1)
1044. western blot problems - western blot troubleshooting (reply: 3)
1045. Excess acrylamide in wells - (reply: 31)
1046. Western Blotting - (reply: 1)
1047. disappeared signal/blank film - (reply: 6)
1048. Endogenously Biotinylated Proteins - (reply: 3)
1049. sds page - (reply: 1)
1050. Gel mix, acrylamide/bis, Ammonium persulfate, etc disposal - (reply: 1)

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