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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1021. freezing after reducing and boiling - (reply: 10)
1022. Invitrogen iBlot - (reply: 6)
1023. Even volumes of different samples - (reply: 3)
1024. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
1025. A single band on WB smaller than expected - (reply: 3)
1026. Ponceau staining after primary and secondary? - (reply: 5)
1027. caspase3 detection - help (reply: 5)
1028. Big white spots on PVDF membrane - (reply: 1)
1029. High background.... - (reply: 6)
1030. WB does not correspond to protein array - (reply: 1)
1031. Strange pattern after transfer - (reply: 8)
1032. Quantifying Western blots - (reply: 1)
1033. Complete transfer, can it be done or not? - (reply: 4)
1034. Can add new secondary Ab? - (reply: 1)
1035. Dot blot - (reply: 2)
1036. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
1037. anti-phosphotyrosine blot on 2mo old samples? - (reply: 1)
1038. NATIVE PAGE - (reply: 1)
1039. Looking for new endogenous control for WB - (reply: 3)
1040. wash blots between antibody incubations with TB-'SALINE' pH 7.4 - why saline.. why this pH? (reply: 1)
1041. western blot problems - western blot troubleshooting (reply: 3)
1042. Excess acrylamide in wells - (reply: 31)
1043. Western Blotting - (reply: 1)
1044. disappeared signal/blank film - (reply: 6)
1045. Endogenously Biotinylated Proteins - (reply: 3)
1046. sds page - (reply: 1)
1047. Gel mix, acrylamide/bis, Ammonium persulfate, etc disposal - (reply: 1)
1048. direct cell lysate - (reply: 8)
1049. Immunofluorescence and Western Blot don't match - (reply: 5)
1050. Cutting membrane after transfer - (reply: 27)

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