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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1021. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
1022. protein quantification by bradford - (reply: 3)
1023. protein extraction from tumor samples containing matrigel? - (reply: 2)
1024. western blot - western blot (reply: 17)
1025. Amount of protein loaded per well? - (reply: 4)
1026. Casp3 - (reply: 2)
1027. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
1028. taking protein out of membrane - (reply: 1)
1029. prearing gel in advance - (reply: 6)
1030. freezing after reducing and boiling - (reply: 10)
1031. Invitrogen iBlot - (reply: 6)
1032. Even volumes of different samples - (reply: 3)
1033. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
1034. A single band on WB smaller than expected - (reply: 3)
1035. Ponceau staining after primary and secondary? - (reply: 5)
1036. caspase3 detection - help (reply: 5)
1037. Big white spots on PVDF membrane - (reply: 1)
1038. High background.... - (reply: 6)
1039. WB does not correspond to protein array - (reply: 1)
1040. Strange pattern after transfer - (reply: 8)
1041. Quantifying Western blots - (reply: 1)
1042. Complete transfer, can it be done or not? - (reply: 4)
1043. Can add new secondary Ab? - (reply: 1)
1044. Dot blot - (reply: 2)
1045. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
1046. anti-phosphotyrosine blot on 2mo old samples? - (reply: 1)
1047. NATIVE PAGE - (reply: 1)
1048. Looking for new endogenous control for WB - (reply: 3)
1049. wash blots between antibody incubations with TB-'SALINE' pH 7.4 - why saline.. why this pH? (reply: 1)
1050. western blot problems - western blot troubleshooting (reply: 3)

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