Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
242. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
243. Trouble shooting in Far-western blot. - (reply: 1)
244. Transcription/Translation reaction not resolving - (reply: 7)
245. Ponceau red problem: membrane turned red - (reply: 3)
246. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
247. No bands/weird bands on western blot - (reply: 3)
248. Protein ladder not separating - (reply: 6)
249. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
250. Preparing supernatant from cell culture for western blot - (reply: 4)
251. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
252. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
253. Curved bands in Western Blot - (reply: 2)
254. Some bands not coming up, others uneven - (reply: 9)
255. Western blot and Triton X-100 in blocking buffer - (reply: 1)
256. Dot blot translation to WB - (reply: 2)
257. Reference protein give different expression - (reply: 3)
258. Excess betamercaptoethanol during sample preparation - (reply: 4)
259. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
260. Far western blot: probe concentration - (reply: 1)
261. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
262. Low Protein Content In Cell Lysate - (reply: 1)
263. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
264. B- actin band looks like dumbbells! - (reply: 2)
265. western blot explenation - (reply: 8)
266. WB of cellular proteins in human serum - (reply: 2)
267. Western blotting - (reply: 9)
268. How long can I store cell lysis buffer at 4C for? - (reply: 1)
269. Which antibody is good for HA-tag with low background bands? - (reply: 1)
270. Blocking solution mistake, please help! - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37