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241. SDS-Electrophoresis, Dye color change - (reply: 1)
242. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
243. Trouble with equal loading - (reply: 8)
244. how many times striping can b done of the blot - (reply: 9)
245. SDS-PAGE of negatively charged protein - (reply: 4)
246. Protection Lanes? - (reply: 4)
247. Tie-2 westerns high background black lanes - (reply: 13)
248. BSA concentration for blocking - (reply: 5)
249. Identically loaded western bands loose intensity from left to right - (reply: 3)
250. eIF4G western blot - (reply: 3)
251. smear bands in western blot - (reply: 12)
252. Huge background with HT22 cells phophoERK antibody - (reply: 3)
253. How to quantify the protein amount for serum samples ? - (reply: 1)
254. Huge background with HT22 cells phophoERK antibody - (reply: 1)
255. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
256. horizontal smear? - (reply: 2)
257. BSA versus milk in blocking and antibody incubation - (reply: 5)
258. Western blots are 'semi' quantitative? - (reply: 12)
259. bands fuse together - (reply: 1)
260. Phospho-protein detection - (reply: 2)
261. Help on using different antibodies in the same membrane - (reply: 8)
262. weird query for Western Blotting - (reply: 2)
263. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
264. Quick/One-step/automated Western Blot - (reply: 8)
265. Amount of antibody used in Western Blot - (reply: 1)
266. WB Transfer: Voltage vs. Current - (reply: 7)
267. Large protein blot ~400kDa - (reply: 3)
268. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
269. Stripping buffer for nitrocellulose membrane - (reply: 1)
270. PAGE for Separation of DNA - (reply: 3)
242. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
243. Trouble with equal loading - (reply: 8)
244. how many times striping can b done of the blot - (reply: 9)
245. SDS-PAGE of negatively charged protein - (reply: 4)
246. Protection Lanes? - (reply: 4)
247. Tie-2 westerns high background black lanes - (reply: 13)
248. BSA concentration for blocking - (reply: 5)
249. Identically loaded western bands loose intensity from left to right - (reply: 3)
250. eIF4G western blot - (reply: 3)
251. smear bands in western blot - (reply: 12)
252. Huge background with HT22 cells phophoERK antibody - (reply: 3)
253. How to quantify the protein amount for serum samples ? - (reply: 1)
254. Huge background with HT22 cells phophoERK antibody - (reply: 1)
255. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
256. horizontal smear? - (reply: 2)
257. BSA versus milk in blocking and antibody incubation - (reply: 5)
258. Western blots are 'semi' quantitative? - (reply: 12)
259. bands fuse together - (reply: 1)
260. Phospho-protein detection - (reply: 2)
261. Help on using different antibodies in the same membrane - (reply: 8)
262. weird query for Western Blotting - (reply: 2)
263. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
264. Quick/One-step/automated Western Blot - (reply: 8)
265. Amount of antibody used in Western Blot - (reply: 1)
266. WB Transfer: Voltage vs. Current - (reply: 7)
267. Large protein blot ~400kDa - (reply: 3)
268. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
269. Stripping buffer for nitrocellulose membrane - (reply: 1)
270. PAGE for Separation of DNA - (reply: 3)