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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Western Blot v ELISA for Different Samples? - (reply: 1)
242. Premixing gel solutions - (reply: 2)
243. getting negative protein measurements - (reply: 3)
244. Peculiar background problem with MPM-2 antibodies - (reply: 2)
245. Stripping the membrane for LiCor - (reply: 1)
246. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
247. Lysis buffer - (reply: 2)
248. SDS Page and WB: Wrong size bands - (reply: 2)
249. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
250. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
251. How to avoid gel breakage after electrophoresis - (reply: 5)
252. What is an Input in SDS-PAGE? - (reply: 1)
253. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
254. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
255. Good loading control for native gels? - (reply: 1)
256. Please help with odd bands and smears on Western blot - (reply: 4)
257. How to load equal amount of protein without using spectramax - (reply: 4)
258. Western blot membrane imaging - (reply: 7)
259. Detection of T7 RNA polymerase by WB - (reply: 6)
260. Shift in the position of band onto the western blot - (reply: 3)
261. Ghost and inverse bands and high background. Help! - (reply: 3)
262. No resolving of protein/bands below 50kDa :-s - (reply: 5)
263. Problems with wet transfer blot - (reply: 2)
264. 5% BSA blocking buffer - (reply: 3)
265. Storage of Membrane before addition of primary antibody - (reply: 3)
266. "Mickey Mouse" bands - (reply: 5)
267. SDS-PAGE : when the transfer stops - (reply: 1)
268. confusing Western results - (reply: 5)
269. aditional band on WB - (reply: 1)
270. Transferring >150 kDA proteins conditions - (reply: 3)

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