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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
242. Uneven lane widths during western blots - (reply: 10)
243. Using human serum as first antibody in Western/ELISA - (reply: 3)
244. Strip Western Blotting Troubleshooting - (reply: 2)
245. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
246. Phospho-ATF-2 coming up different sizes - (reply: 1)
247. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
248. A possible protocol for western blotting troubleshooting? - (reply: 1)
249. Vertical Vs horizontal electrophoresis - (reply: 3)
250. Reprobing with the same antibody - (reply: 3)
251. SDS-PAGE problem with staining yellow background - (reply: 3)
252. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
253. Western blot narrow bands - (reply: 1)
254. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
255. Western Blot Antibody Epitope - (reply: 1)
256. Western blot band anaylisis - (reply: 3)
257. protein aggregation (?) in gelatin zymography - (reply: 1)
258. Use of wrong TBS concentration - (reply: 1)
259. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
260. SDS-PAGE staining for digestion assay - (reply: 1)
261. Can I improve my primary antibody? - (reply: 2)
262. got band in control positive but not in sample... - (reply: 2)
263. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
264. Problems with IPs - (reply: 2)
265. Band is at the wrong size, but same for all antibodies - (reply: 6)
266. Problems with western blot detection - (reply: 1)
267. Methods of concentrating total protein extracts - (reply: 2)
268. Using serum as the sample in Western blot - (reply: 3)
269. Puzzeling western blot results, missing band - (reply: 3)
270. Secondary AB blocking - (reply: 1)

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