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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Discrepancy between WB and Flow cytometry result - (reply: 5)
242. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
243. Western blotting transfer - (reply: 2)
244. Western Quantification (No BCA???) - (reply: 2)
245. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
246. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
247. Trouble shooting in Far-western blot. - (reply: 1)
248. Transcription/Translation reaction not resolving - (reply: 7)
249. Ponceau red problem: membrane turned red - (reply: 3)
250. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
251. No bands/weird bands on western blot - (reply: 3)
252. Protein ladder not separating - (reply: 6)
253. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
254. Preparing supernatant from cell culture for western blot - (reply: 4)
255. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
256. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
257. Curved bands in Western Blot - (reply: 2)
258. Some bands not coming up, others uneven - (reply: 9)
259. Western blot and Triton X-100 in blocking buffer - (reply: 1)
260. Dot blot translation to WB - (reply: 2)
261. Reference protein give different expression - (reply: 3)
262. Excess betamercaptoethanol during sample preparation - (reply: 4)
263. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
264. Far western blot: probe concentration - (reply: 1)
265. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
266. Low Protein Content In Cell Lysate - (reply: 1)
267. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
268. B- actin band looks like dumbbells! - (reply: 2)
269. western blot explenation - (reply: 8)
270. WB of cellular proteins in human serum - (reply: 2)

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