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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Non-specific bands and fuzzy bands - (reply: 1)
242. protein showing up around 50 kda rather than 20 kda - (reply: 2)
243. Help for western blot wet transfer blurry bands - (reply: 2)
244. anti-BRCA1 antibody specificity issues - (reply: 2)
245. Concentrating a diluted protein and protein determination - (reply: 4)
246. Blurry bands ... - (reply: 12)
247. Dot blotting problems - (reply: 3)
248. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
249. Uneven lane widths during western blots - (reply: 10)
250. Using human serum as first antibody in Western/ELISA - (reply: 3)
251. Strip Western Blotting Troubleshooting - (reply: 2)
252. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
253. Phospho-ATF-2 coming up different sizes - (reply: 1)
254. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
255. A possible protocol for western blotting troubleshooting? - (reply: 1)
256. Vertical Vs horizontal electrophoresis - (reply: 3)
257. Reprobing with the same antibody - (reply: 3)
258. SDS-PAGE problem with staining yellow background - (reply: 3)
259. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
260. Western blot narrow bands - (reply: 1)
261. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
262. Western Blot Antibody Epitope - (reply: 1)
263. Western blot band anaylisis - (reply: 3)
264. protein aggregation (?) in gelatin zymography - (reply: 1)
265. Use of wrong TBS concentration - (reply: 1)
266. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
267. SDS-PAGE staining for digestion assay - (reply: 1)
268. Can I improve my primary antibody? - (reply: 2)
269. got band in control positive but not in sample... - (reply: 2)
270. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)

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