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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Using human serum as first antibody in Western/ELISA - (reply: 3)
242. Strip Western Blotting Troubleshooting - (reply: 2)
243. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
244. Phospho-ATF-2 coming up different sizes - (reply: 1)
245. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
246. A possible protocol for western blotting troubleshooting? - (reply: 1)
247. Vertical Vs horizontal electrophoresis - (reply: 3)
248. Reprobing with the same antibody - (reply: 3)
249. SDS-PAGE problem with staining yellow background - (reply: 3)
250. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
251. Western blot narrow bands - (reply: 1)
252. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
253. Western Blot Antibody Epitope - (reply: 1)
254. Western blot band anaylisis - (reply: 3)
255. protein aggregation (?) in gelatin zymography - (reply: 1)
256. Use of wrong TBS concentration - (reply: 1)
257. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
258. SDS-PAGE staining for digestion assay - (reply: 1)
259. Can I improve my primary antibody? - (reply: 2)
260. got band in control positive but not in sample... - (reply: 2)
261. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
262. Problems with IPs - (reply: 2)
263. Band is at the wrong size, but same for all antibodies - (reply: 6)
264. Problems with western blot detection - (reply: 1)
265. Methods of concentrating total protein extracts - (reply: 2)
266. Using serum as the sample in Western blot - (reply: 3)
267. Puzzeling western blot results, missing band - (reply: 3)
268. Secondary AB blocking - (reply: 1)
269. western blood immunodetection Ab - (reply: 1)
270. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)

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