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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. TAU gel electrophoresis of histones - (reply: 4)
242. Bands dark on sides and light in middle - (reply: 7)
243. Addition of sample loading buffer, before or after heating - (reply: 5)
244. question about protein expression normalization. - (reply: 6)
245. Protein bands not migrating - (reply: 3)
246. Help with high background (have tried most trouble shooting) - (reply: 8)
247. Tumor protein precipitation at denaturing step? - (reply: 1)
248. How to prepare blood serum for western blot? - (reply: 3)
249. TNF alpha and IL 1b Western Blot - (reply: 1)
250. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
251. SDS-PAGE trouble with protein concentration - (reply: 2)
252. Acrylamid - (reply: 5)
253. Detecting doublet bands in Western for eGFP control - (reply: 1)
254. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
255. Protein Size 50 kDa higher than expected - (reply: 3)
256. Acid urea gel electrophoresis (AUGE) - (reply: 7)
257. Phospho-protein vs total protein Western blot - (reply: 5)
258. storing the loaded gel before running it - (reply: 7)
259. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
260. Western blot trouble shooting - (reply: 3)
261. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
262. two bands on WB-alternative use of start codons? - (reply: 2)
263. TCA Precipitation to remove albumin - (reply: 6)
264. adjusting pH of 4x stacking buffer - (reply: 3)
265. No WB bands for H2AX at all...! - (reply: 10)
266. any tips for doing Fluorescence Western Blot? - (reply: 2)
267. Unknown silver/grey residue left after transfer. - (reply: 2)
268. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
269. Changes to housekeeping loading controls after drug treatment - (reply: 2)
270. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)

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