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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. can coommassie stained gel be western blot - (reply: 5)
242. Wet transfer: western blot - (reply: 3)
243. Reprobing a PVDF membrane which has gone dry - (reply: 1)
244. Non-specific bands and fuzzy bands - (reply: 1)
245. protein showing up around 50 kda rather than 20 kda - (reply: 2)
246. Help for western blot wet transfer blurry bands - (reply: 2)
247. anti-BRCA1 antibody specificity issues - (reply: 2)
248. Concentrating a diluted protein and protein determination - (reply: 4)
249. Blurry bands ... - (reply: 12)
250. Dot blotting problems - (reply: 3)
251. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
252. Uneven lane widths during western blots - (reply: 10)
253. Using human serum as first antibody in Western/ELISA - (reply: 3)
254. Strip Western Blotting Troubleshooting - (reply: 2)
255. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
256. Phospho-ATF-2 coming up different sizes - (reply: 1)
257. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
258. A possible protocol for western blotting troubleshooting? - (reply: 1)
259. Vertical Vs horizontal electrophoresis - (reply: 3)
260. Reprobing with the same antibody - (reply: 3)
261. SDS-PAGE problem with staining yellow background - (reply: 3)
262. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
263. Western blot narrow bands - (reply: 1)
264. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
265. Western Blot Antibody Epitope - (reply: 1)
266. Western blot band anaylisis - (reply: 3)
267. protein aggregation (?) in gelatin zymography - (reply: 1)
268. Use of wrong TBS concentration - (reply: 1)
269. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
270. SDS-PAGE staining for digestion assay - (reply: 1)

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