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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Western blot and Triton X-100 in blocking buffer - (reply: 1)
242. Dot blot translation to WB - (reply: 2)
243. Reference protein give different expression - (reply: 3)
244. Excess betamercaptoethanol during sample preparation - (reply: 4)
245. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
246. Far western blot: probe concentration - (reply: 1)
247. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
248. Low Protein Content In Cell Lysate - (reply: 1)
249. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
250. B- actin band looks like dumbbells! - (reply: 2)
251. western blot explenation - (reply: 8)
252. WB of cellular proteins in human serum - (reply: 2)
253. Western blotting - (reply: 9)
254. How long can I store cell lysis buffer at 4C for? - (reply: 1)
255. Which antibody is good for HA-tag with low background bands? - (reply: 1)
256. Blocking solution mistake, please help! - (reply: 2)
257. Membrane Protein SDS PAGE and WB - (reply: 1)
258. Wet transfer of proteins between 100-200kDa - (reply: 2)
259. western blot and actin issue - (reply: 2)
260. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)
261. conjugated antibodies in immunoprecipitation - (reply: 3)
262. Separating proteing with difference of 5Kd on western blot - (reply: 4)
263. small proteins - (reply: 2)
264. SDS PAGE gel storing - (reply: 3)
265. Interpretation & Troubleshooting on SDS/Native PAGE - (reply: 5)
266. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
267. Blocking Buffer gone bad - (reply: 1)
268. Running time is excessively long, bunched ladder - (reply: 2)
269. Trouble estimating my protein size using ladder (image included) - (reply: 2)
270. Disc SDS PAGE on Multiphor II system - (reply: 3)

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