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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
241. Uneven beta actin - (reply: 2)
242. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
243. NCOR1 western - (reply: 8)
244. Discrepancy between WB and Flow cytometry result - (reply: 5)
245. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
246. Western blotting transfer - (reply: 2)
247. Western Quantification (No BCA???) - (reply: 2)
248. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
249. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
250. Trouble shooting in Far-western blot. - (reply: 1)
251. Transcription/Translation reaction not resolving - (reply: 7)
252. Ponceau red problem: membrane turned red - (reply: 3)
253. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
254. No bands/weird bands on western blot - (reply: 3)
255. Protein ladder not separating - (reply: 6)
256. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
257. Preparing supernatant from cell culture for western blot - (reply: 4)
258. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
259. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
260. Curved bands in Western Blot - (reply: 2)
261. Some bands not coming up, others uneven - (reply: 9)
262. Western blot and Triton X-100 in blocking buffer - (reply: 1)
263. Dot blot translation to WB - (reply: 2)
264. Reference protein give different expression - (reply: 3)
265. Excess betamercaptoethanol during sample preparation - (reply: 4)
266. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
267. Far western blot: probe concentration - (reply: 1)
268. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
269. Low Protein Content In Cell Lysate - (reply: 1)
270. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)

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