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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
91. After Co Ip can Iuse SDS PAGE - (reply: 1)
92. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
93. BCIP/NBT developed blot disappearing - (reply: 1)
94. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
95. how to choose a proper tag for western blotting - (reply: 6)
96. Problem with thawed samples - (reply: 3)
97. Problems with histones in SDS-PAGE - (reply: 1)
98. Stripping problem - (reply: 5)
99. Problems of beta-actin - (reply: 1)
100. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
101. Problems of western signal - (reply: 5)
102. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
103. 2x laemmli buffer - (reply: 4)
104. Relative humidity in the electrophoresis lab - (reply: 3)
105. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
106. how can i explain the different pattern from my labmate's one - (reply: 8)
107. Ladder and proteins do not migrate into stacking gel - (reply: 5)
108. Western Blot: Unequal Sample Migration - (reply: 4)
109. Western blot with smeared bands - (reply: 3)
110. SDS PAGE sample preparation question, and discussion? - (reply: 3)
111. too much heating LDS-sample buffer - (reply: 3)
112. problem of cell lysate - (reply: 3)
113. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
114. Blocking agent in western blotting of histone proteins - (reply: 1)
115. Best western loading control for comparing different cell lines - (reply: 2)
116. western blotting running problem? - (reply: 2)
117. Keeping apoptotic/detached cells for WB? - (reply: 3)
118. Advice with WB please - (reply: 3)
119. Western Blot Wet Transfer Conditions - (reply: 10)
120. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)

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