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91. aditional band on WB - (reply: 1)
92. Transferring >150 kDA proteins conditions - (reply: 3)
93. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
94. TAU gel electrophoresis of histones - (reply: 4)
95. Bands dark on sides and light in middle - (reply: 7)
96. Addition of sample loading buffer, before or after heating - (reply: 5)
97. question about protein expression normalization. - (reply: 6)
98. Protein bands not migrating - (reply: 3)
99. Help with high background (have tried most trouble shooting) - (reply: 8)
100. Tumor protein precipitation at denaturing step? - (reply: 1)
101. How to prepare blood serum for western blot? - (reply: 3)
102. TNF alpha and IL 1b Western Blot - (reply: 1)
103. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
104. SDS-PAGE trouble with protein concentration - (reply: 2)
105. Acrylamid - (reply: 5)
106. Detecting doublet bands in Western for eGFP control - (reply: 1)
107. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
108. Protein Size 50 kDa higher than expected - (reply: 3)
109. Acid urea gel electrophoresis (AUGE) - (reply: 7)
110. Phospho-protein vs total protein Western blot - (reply: 5)
111. storing the loaded gel before running it - (reply: 7)
112. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
113. Western blot trouble shooting - (reply: 3)
114. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
115. two bands on WB-alternative use of start codons? - (reply: 2)
116. TCA Precipitation to remove albumin - (reply: 6)
117. adjusting pH of 4x stacking buffer - (reply: 3)
118. No WB bands for H2AX at all...! - (reply: 10)
119. any tips for doing Fluorescence Western Blot? - (reply: 2)
120. Unknown silver/grey residue left after transfer. - (reply: 2)
92. Transferring >150 kDA proteins conditions - (reply: 3)
93. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
94. TAU gel electrophoresis of histones - (reply: 4)
95. Bands dark on sides and light in middle - (reply: 7)
96. Addition of sample loading buffer, before or after heating - (reply: 5)
97. question about protein expression normalization. - (reply: 6)
98. Protein bands not migrating - (reply: 3)
99. Help with high background (have tried most trouble shooting) - (reply: 8)
100. Tumor protein precipitation at denaturing step? - (reply: 1)
101. How to prepare blood serum for western blot? - (reply: 3)
102. TNF alpha and IL 1b Western Blot - (reply: 1)
103. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
104. SDS-PAGE trouble with protein concentration - (reply: 2)
105. Acrylamid - (reply: 5)
106. Detecting doublet bands in Western for eGFP control - (reply: 1)
107. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
108. Protein Size 50 kDa higher than expected - (reply: 3)
109. Acid urea gel electrophoresis (AUGE) - (reply: 7)
110. Phospho-protein vs total protein Western blot - (reply: 5)
111. storing the loaded gel before running it - (reply: 7)
112. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
113. Western blot trouble shooting - (reply: 3)
114. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
115. two bands on WB-alternative use of start codons? - (reply: 2)
116. TCA Precipitation to remove albumin - (reply: 6)
117. adjusting pH of 4x stacking buffer - (reply: 3)
118. No WB bands for H2AX at all...! - (reply: 10)
119. any tips for doing Fluorescence Western Blot? - (reply: 2)
120. Unknown silver/grey residue left after transfer. - (reply: 2)