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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
91. too much heating LDS-sample buffer - (reply: 3)
92. problem of cell lysate - (reply: 3)
93. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
94. Blocking agent in western blotting of histone proteins - (reply: 1)
95. Best western loading control for comparing different cell lines - (reply: 2)
96. western blotting running problem? - (reply: 2)
97. Keeping apoptotic/detached cells for WB? - (reply: 3)
98. Advice with WB please - (reply: 3)
99. Western Blot Wet Transfer Conditions - (reply: 10)
100. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
101. Western Blot and Inmunofluorescence contradictions - (reply: 12)
102. Nanog double band in WB - (reply: 1)
103. My beta actin bands are changing - (reply: 6)
104. ECL Substrate Choices - (reply: 2)
105. Why does my gel have streaks instead of bands? - (reply: 2)
106. Left ECL kit at room temp. Is it ruined? - (reply: 2)
107. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
108. Native PAGE problem - not entering gel enough - (reply: 9)
109. film saturation in immunoblots - (reply: 1)
110. Problems with wells on SDS PAGE gels - (reply: 3)
111. Protein is present, but not detected by antibody - (reply: 4)
112. secondary antibody creation - (reply: 1)
113. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
114. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
115. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
116. Low signal, weird background stains on blot - (reply: 5)
117. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
118. Incomplete Blotting and staining of NC membrane - (reply: 7)
119. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
120. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)

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