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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
91. BCIP/NBT developed blot disappearing - (reply: 1)
92. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
93. how to choose a proper tag for western blotting - (reply: 6)
94. Problem with thawed samples - (reply: 3)
95. Problems with histones in SDS-PAGE - (reply: 1)
96. Stripping problem - (reply: 5)
97. Problems of beta-actin - (reply: 1)
98. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
99. Problems of western signal - (reply: 5)
100. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
101. 2x laemmli buffer - (reply: 4)
102. Relative humidity in the electrophoresis lab - (reply: 3)
103. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
104. how can i explain the different pattern from my labmate's one - (reply: 8)
105. Ladder and proteins do not migrate into stacking gel - (reply: 5)
106. Western Blot: Unequal Sample Migration - (reply: 4)
107. Western blot with smeared bands - (reply: 3)
108. SDS PAGE sample preparation question, and discussion? - (reply: 3)
109. too much heating LDS-sample buffer - (reply: 3)
110. problem of cell lysate - (reply: 3)
111. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
112. Blocking agent in western blotting of histone proteins - (reply: 1)
113. Best western loading control for comparing different cell lines - (reply: 2)
114. western blotting running problem? - (reply: 2)
115. Keeping apoptotic/detached cells for WB? - (reply: 3)
116. Advice with WB please - (reply: 3)
117. Western Blot Wet Transfer Conditions - (reply: 10)
118. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
119. Western Blot and Inmunofluorescence contradictions - (reply: 12)
120. Nanog double band in WB - (reply: 1)

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