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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
91. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
92. Problem with Denative lysis - (reply: 1)
93. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
94. Polyclonal antibody precipitating - (reply: 1)
95. manual 2D electrophoresis - (reply: 8)
96. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
97. sample sonication before loading - (reply: 6)
98. transfer of low molecular weight protein - (reply: 3)
99. How is the mass detected via SDS-PAGE? - (reply: 3)
100. Water in beads during boiling - (reply: 3)
101. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
102. Runing problems - (reply: 18)
103. Western Blotting of Amyloid Beta - (reply: 1)
104. 10x Laemmli buffer - (reply: 5)
105. Need help translating my western blot protocol into every language - (reply: 3)
106. Semi-dry system for NuPage with MES buffer - (reply: 4)
107. Recommend anti-rat HRP secondary antibody - (reply: 2)
108. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
109. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
110. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
111. Western blot to CD44? - (reply: 3)
112. Amount of EDTA - (reply: 4)
113. SDS Apparatus for northern - (reply: 1)
114. Prey protein band bleeds, IgG band normal? - (reply: 3)
115. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
116. His-Probe - (reply: 2)
117. Problems with western membrane stripping - (reply: 2)
118. Can protein isomers be detected in SDS-PAGE - (reply: 3)
119. western blot of cardiomyocytes - (reply: 2)
120. Gradient Gel Electrophoresis - (reply: 3)

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