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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
91. Stripping problem - (reply: 5)
92. Problems of beta-actin - (reply: 1)
93. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
94. Problems of western signal - (reply: 5)
95. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
96. 2x laemmli buffer - (reply: 4)
97. Relative humidity in the electrophoresis lab - (reply: 3)
98. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
99. how can i explain the different pattern from my labmate's one - (reply: 8)
100. Ladder and proteins do not migrate into stacking gel - (reply: 5)
101. Western Blot: Unequal Sample Migration - (reply: 4)
102. Western blot with smeared bands - (reply: 3)
103. SDS PAGE sample preparation question, and discussion? - (reply: 3)
104. too much heating LDS-sample buffer - (reply: 3)
105. problem of cell lysate - (reply: 3)
106. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
107. Blocking agent in western blotting of histone proteins - (reply: 1)
108. Best western loading control for comparing different cell lines - (reply: 2)
109. western blotting running problem? - (reply: 2)
110. Keeping apoptotic/detached cells for WB? - (reply: 3)
111. Advice with WB please - (reply: 3)
112. Western Blot Wet Transfer Conditions - (reply: 10)
113. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
114. Western Blot and Inmunofluorescence contradictions - (reply: 12)
115. Nanog double band in WB - (reply: 1)
116. My beta actin bands are changing - (reply: 6)
117. ECL Substrate Choices - (reply: 2)
118. Why does my gel have streaks instead of bands? - (reply: 2)
119. Left ECL kit at room temp. Is it ruined? - (reply: 2)
120. what do you think is the problem with this polyacrylamide gel? - (reply: 2)

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