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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
91. Problem with thawed samples - (reply: 3)
92. Problems with histones in SDS-PAGE - (reply: 1)
93. Stripping problem - (reply: 5)
94. Problems of beta-actin - (reply: 1)
95. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
96. Problems of western signal - (reply: 5)
97. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
98. 2x laemmli buffer - (reply: 4)
99. Relative humidity in the electrophoresis lab - (reply: 3)
100. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
101. how can i explain the different pattern from my labmate's one - (reply: 8)
102. Ladder and proteins do not migrate into stacking gel - (reply: 5)
103. Western Blot: Unequal Sample Migration - (reply: 4)
104. Western blot with smeared bands - (reply: 3)
105. SDS PAGE sample preparation question, and discussion? - (reply: 3)
106. too much heating LDS-sample buffer - (reply: 3)
107. problem of cell lysate - (reply: 3)
108. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
109. Blocking agent in western blotting of histone proteins - (reply: 1)
110. Best western loading control for comparing different cell lines - (reply: 2)
111. western blotting running problem? - (reply: 2)
112. Keeping apoptotic/detached cells for WB? - (reply: 3)
113. Advice with WB please - (reply: 3)
114. Western Blot Wet Transfer Conditions - (reply: 10)
115. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
116. Western Blot and Inmunofluorescence contradictions - (reply: 12)
117. Nanog double band in WB - (reply: 1)
118. My beta actin bands are changing - (reply: 6)
119. ECL Substrate Choices - (reply: 2)
120. Why does my gel have streaks instead of bands? - (reply: 2)

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