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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
961. my membranes are blue! - silly mistake, what to do? (reply: 1)
962. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
963. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
964. Primary Antibody for SPDEF - (reply: 1)
965. Detecting Patterns of phosphorylated protein - (reply: 1)
966. Sample Diffusion Before Applying Current - (reply: 4)
967. IP and western blot - (reply: 5)
968. Semi-dry transfer conditions - Volts or Amps (reply: 1)
969. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
970. Destain after PoneauS - (reply: 6)
971. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
972. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
973. Variability in GAPDH - (reply: 5)
974. nitrocellulose or PVDF - (reply: 7)
975. crappy bands after developing - (reply: 3)
976. cloudy gel - (reply: 2)
977. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
978. Beginner Question - (reply: 6)
979. What's the problem with this SDS-PAGE? - (reply: 8)
980. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
981. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
982. Stripping question - (reply: 3)
983. Smears after transfer - (reply: 2)
984. Accidentally Froze Transfer Buffer - (reply: 1)
985. ImageJ analysis on IFs - (reply: 11)
986. Equal loading for Supernatant western blots??? - (reply: 1)
987. Ponceau Staining Question - (reply: 1)
988. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
989. bubbles between gel and glass plate - (reply: 9)
990. Want to detect oligomers - Need advice on native PAGE (reply: 2)

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