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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
961. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
962. Primary Antibody for SPDEF - (reply: 1)
963. Detecting Patterns of phosphorylated protein - (reply: 1)
964. Sample Diffusion Before Applying Current - (reply: 4)
965. IP and western blot - (reply: 5)
966. Semi-dry transfer conditions - Volts or Amps (reply: 1)
967. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
968. Destain after PoneauS - (reply: 6)
969. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
970. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
971. Variability in GAPDH - (reply: 5)
972. nitrocellulose or PVDF - (reply: 7)
973. crappy bands after developing - (reply: 3)
974. cloudy gel - (reply: 2)
975. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
976. Beginner Question - (reply: 6)
977. What's the problem with this SDS-PAGE? - (reply: 8)
978. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
979. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
980. Stripping question - (reply: 3)
981. Smears after transfer - (reply: 2)
982. Accidentally Froze Transfer Buffer - (reply: 1)
983. ImageJ analysis on IFs - (reply: 11)
984. Equal loading for Supernatant western blots??? - (reply: 1)
985. Ponceau Staining Question - (reply: 1)
986. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
987. bubbles between gel and glass plate - (reply: 9)
988. Want to detect oligomers - Need advice on native PAGE (reply: 2)
989. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
990. Primary antibody incubation length - (reply: 3)

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