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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
961. lost signal - (reply: 4)
962. Using NaN3 to quench HRP conjugated secondary Ab - (reply: 1)
963. White speckles on western blots - Why?? :( (reply: 3)
964. Blocking buffer as antibody dilutent? - (reply: 4)
965. How to prepare colloidal coomassie blue? - ingredients can not dissolve (reply: 4)
966. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
967. protein quantification by bradford - (reply: 3)
968. protein extraction from tumor samples containing matrigel? - (reply: 2)
969. western blot - western blot (reply: 17)
970. Amount of protein loaded per well? - (reply: 4)
971. Casp3 - (reply: 2)
972. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
973. taking protein out of membrane - (reply: 1)
974. prearing gel in advance - (reply: 6)
975. freezing after reducing and boiling - (reply: 10)
976. Invitrogen iBlot - (reply: 6)
977. Even volumes of different samples - (reply: 3)
978. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
979. A single band on WB smaller than expected - (reply: 3)
980. Ponceau staining after primary and secondary? - (reply: 5)
981. caspase3 detection - help (reply: 5)
982. Big white spots on PVDF membrane - (reply: 1)
983. High background.... - (reply: 6)
984. WB does not correspond to protein array - (reply: 1)
985. Strange pattern after transfer - (reply: 8)
986. Quantifying Western blots - (reply: 1)
987. Complete transfer, can it be done or not? - (reply: 4)
988. Can add new secondary Ab? - (reply: 1)
989. Dot blot - (reply: 2)
990. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)

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