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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
961. Sample Diffusion Before Applying Current - (reply: 4)
962. IP and western blot - (reply: 5)
963. Semi-dry transfer conditions - Volts or Amps (reply: 1)
964. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
965. Destain after PoneauS - (reply: 6)
966. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
967. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
968. Variability in GAPDH - (reply: 5)
969. nitrocellulose or PVDF - (reply: 7)
970. crappy bands after developing - (reply: 3)
971. cloudy gel - (reply: 2)
972. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
973. Beginner Question - (reply: 6)
974. What's the problem with this SDS-PAGE? - (reply: 8)
975. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
976. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
977. Stripping question - (reply: 3)
978. Smears after transfer - (reply: 2)
979. Accidentally Froze Transfer Buffer - (reply: 1)
980. ImageJ analysis on IFs - (reply: 11)
981. Equal loading for Supernatant western blots??? - (reply: 1)
982. Ponceau Staining Question - (reply: 1)
983. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
984. bubbles between gel and glass plate - (reply: 9)
985. Want to detect oligomers - Need advice on native PAGE (reply: 2)
986. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
987. Primary antibody incubation length - (reply: 3)
988. How to extract protein from SDS-PAGE gel? - (reply: 7)
989. colorimetry vs chemiluminescence - (reply: 1)
990. antribody gone old? - (reply: 11)

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