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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
961. Semi-dry transfer conditions - Volts or Amps (reply: 1)
962. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
963. Destain after PoneauS - (reply: 6)
964. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
965. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
966. Variability in GAPDH - (reply: 5)
967. nitrocellulose or PVDF - (reply: 7)
968. crappy bands after developing - (reply: 3)
969. cloudy gel - (reply: 2)
970. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
971. Beginner Question - (reply: 6)
972. What's the problem with this SDS-PAGE? - (reply: 8)
973. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
974. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
975. Stripping question - (reply: 3)
976. Smears after transfer - (reply: 2)
977. Accidentally Froze Transfer Buffer - (reply: 1)
978. ImageJ analysis on IFs - (reply: 11)
979. Equal loading for Supernatant western blots??? - (reply: 1)
980. Ponceau Staining Question - (reply: 1)
981. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
982. bubbles between gel and glass plate - (reply: 9)
983. Want to detect oligomers - Need advice on native PAGE (reply: 2)
984. 2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots" (reply: 3)
985. Primary antibody incubation length - (reply: 3)
986. How to extract protein from SDS-PAGE gel? - (reply: 7)
987. colorimetry vs chemiluminescence - (reply: 1)
988. antribody gone old? - (reply: 11)
989. Yellow Laemmli sample buffer - (reply: 6)
990. Antibody Characterization - KO vs. WT or Heterologous overexpression (reply: 8)

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