|
961. pellet became insoluble after boiling. - (reply: 4)
962. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
963. Ladder but no protein - (reply: 4)
964. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
965. my membranes are blue! - silly mistake, what to do? (reply: 1)
966. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
967. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
968. Primary Antibody for SPDEF - (reply: 1)
969. Detecting Patterns of phosphorylated protein - (reply: 1)
970. Sample Diffusion Before Applying Current - (reply: 4)
971. IP and western blot - (reply: 5)
972. Semi-dry transfer conditions - Volts or Amps (reply: 1)
973. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
974. Destain after PoneauS - (reply: 6)
975. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
976. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
977. Variability in GAPDH - (reply: 5)
978. nitrocellulose or PVDF - (reply: 7)
979. crappy bands after developing - (reply: 3)
980. cloudy gel - (reply: 2)
981. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
982. Beginner Question - (reply: 6)
983. What's the problem with this SDS-PAGE? - (reply: 8)
984. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
985. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
986. Stripping question - (reply: 3)
987. Smears after transfer - (reply: 2)
988. Accidentally Froze Transfer Buffer - (reply: 1)
989. ImageJ analysis on IFs - (reply: 11)
990. Equal loading for Supernatant western blots??? - (reply: 1)
962. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
963. Ladder but no protein - (reply: 4)
964. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
965. my membranes are blue! - silly mistake, what to do? (reply: 1)
966. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
967. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
968. Primary Antibody for SPDEF - (reply: 1)
969. Detecting Patterns of phosphorylated protein - (reply: 1)
970. Sample Diffusion Before Applying Current - (reply: 4)
971. IP and western blot - (reply: 5)
972. Semi-dry transfer conditions - Volts or Amps (reply: 1)
973. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
974. Destain after PoneauS - (reply: 6)
975. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
976. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
977. Variability in GAPDH - (reply: 5)
978. nitrocellulose or PVDF - (reply: 7)
979. crappy bands after developing - (reply: 3)
980. cloudy gel - (reply: 2)
981. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
982. Beginner Question - (reply: 6)
983. What's the problem with this SDS-PAGE? - (reply: 8)
984. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
985. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
986. Stripping question - (reply: 3)
987. Smears after transfer - (reply: 2)
988. Accidentally Froze Transfer Buffer - (reply: 1)
989. ImageJ analysis on IFs - (reply: 11)
990. Equal loading for Supernatant western blots??? - (reply: 1)