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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
961. Ladder but no protein - (reply: 4)
962. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
963. my membranes are blue! - silly mistake, what to do? (reply: 1)
964. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
965. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
966. Primary Antibody for SPDEF - (reply: 1)
967. Detecting Patterns of phosphorylated protein - (reply: 1)
968. Sample Diffusion Before Applying Current - (reply: 4)
969. IP and western blot - (reply: 5)
970. Semi-dry transfer conditions - Volts or Amps (reply: 1)
971. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
972. Destain after PoneauS - (reply: 6)
973. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
974. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
975. Variability in GAPDH - (reply: 5)
976. nitrocellulose or PVDF - (reply: 7)
977. crappy bands after developing - (reply: 3)
978. cloudy gel - (reply: 2)
979. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
980. Beginner Question - (reply: 6)
981. What's the problem with this SDS-PAGE? - (reply: 8)
982. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
983. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
984. Stripping question - (reply: 3)
985. Smears after transfer - (reply: 2)
986. Accidentally Froze Transfer Buffer - (reply: 1)
987. ImageJ analysis on IFs - (reply: 11)
988. Equal loading for Supernatant western blots??? - (reply: 1)
989. Ponceau Staining Question - (reply: 1)
990. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)

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