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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Bands disappear after longer exposure - (reply: 4)
512. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
513. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
514. Antibody Optimization - (reply: 2)
515. Protein agregation in WB samples - (reply: 3)
516. Same Non-specific bands show up for different Abs!! - (reply: 7)
517. what caused bubble band of my WB? - (reply: 4)
518. blotting detection - (reply: 3)
519. Max amount of protein - (reply: 4)
520. Areas of membrane bleached - (reply: 1)
521. asking suggestions for western blot - (reply: 3)
522. Can i apply primary antibody for two nights instead of one? - (reply: 3)
523. Strange bands and large smear in western blot - (reply: 4)
524. Freezing proteins at -80 - (reply: 1)
525. Tris glycine SDS buffer prep pH error - (reply: 2)
526. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
527. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
528. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
529. problems with negative control - (reply: 2)
530. urgent-W.B visualisation on kodak film - (reply: 2)
531. NP 40 lysis buffer - (reply: 1)
532. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
533. Dark western blot film - (reply: 12)
534. how to get red of high secondary signal - (reply: 4)
535. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
536. Detecting phosphoproteins - different results when samples rerun (reply: 1)
537. Is loading buffer ok? - (reply: 1)
538. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
539. Antibody vs Antigen concentration effect - (reply: 3)
540. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)

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