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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Samples leaking out of wells in gel - (reply: 2)
512. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
513. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
514. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
515. Strange E.coli growth - (reply: 3)
516. Problem with Immunoprecipitated samples - (reply: 2)
517. problems in transfer - (reply: 3)
518. Phospho-JNK - (reply: 9)
519. Bradford reagent for staining gels? - (reply: 1)
520. developing film manually - (reply: 19)
521. non specific bands - (reply: 7)
522. BSA vs Milk - (reply: 2)
523. Western Blot no target bands, show Non-specific bands - (reply: 5)
524. how critical is the pH of tbst? - (reply: 2)
525. Fc fusion protein smearing on SDS PAGE - (reply: 2)
526. what do you recommend for Semi-Dry unit - (reply: 2)
527. Bands disappear after longer exposure - (reply: 4)
528. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
529. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
530. Antibody Optimization - (reply: 2)
531. Protein agregation in WB samples - (reply: 3)
532. Same Non-specific bands show up for different Abs!! - (reply: 7)
533. what caused bubble band of my WB? - (reply: 4)
534. blotting detection - (reply: 3)
535. Max amount of protein - (reply: 4)
536. Areas of membrane bleached - (reply: 1)
537. asking suggestions for western blot - (reply: 3)
538. Can i apply primary antibody for two nights instead of one? - (reply: 3)
539. Strange bands and large smear in western blot - (reply: 4)
540. Freezing proteins at -80 - (reply: 1)

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