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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Nitrocellulose membrane storage after transfer - (reply: 6)
512. western without tween - (reply: 3)
513. Bubbles on the marker after transfer - (reply: 2)
514. Missing patches on immunoblot - please help! - (reply: 7)
515. stupid question - (reply: 8)
516. GAPDH merging lanes - (reply: 1)
517. Band at same position using different primary antibodies - (reply: 3)
518. Western for very small protein - (reply: 3)
519. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
520. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
521. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
522. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
523. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
524. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
525. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
526. What cause "downshift" in SDS-PAGE? - (reply: 5)
527. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
528. Blue native PAGE problems - (reply: 1)
529. running problems SDS-PAGE gel (zymography) - (reply: 4)
530. Western unresolved lanes - (reply: 2)
531. Doing WB from scratch for the first time. Need some info. - (reply: 2)
532. Western blot bovine collagen typ I problem - (reply: 3)
533. no band, extremely clean blot - (reply: 1)
534. Acrylamide stock - (reply: 2)
535. low signal in WB - (reply: 3)
536. standard internal marker for quantification - (reply: 3)
537. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
538. IgG - unspecific band (reply: 3)
539. red ponceau - (reply: 3)
540. Noisy fluorescent gels - (reply: 1)

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