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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Fc fusion protein smearing on SDS PAGE - (reply: 2)
512. what do you recommend for Semi-Dry unit - (reply: 2)
513. Bands disappear after longer exposure - (reply: 4)
514. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
515. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
516. Antibody Optimization - (reply: 2)
517. Protein agregation in WB samples - (reply: 3)
518. Same Non-specific bands show up for different Abs!! - (reply: 7)
519. what caused bubble band of my WB? - (reply: 4)
520. blotting detection - (reply: 3)
521. Max amount of protein - (reply: 4)
522. Areas of membrane bleached - (reply: 1)
523. asking suggestions for western blot - (reply: 3)
524. Can i apply primary antibody for two nights instead of one? - (reply: 3)
525. Strange bands and large smear in western blot - (reply: 4)
526. Freezing proteins at -80 - (reply: 1)
527. Tris glycine SDS buffer prep pH error - (reply: 2)
528. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
529. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
530. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
531. problems with negative control - (reply: 2)
532. urgent-W.B visualisation on kodak film - (reply: 2)
533. NP 40 lysis buffer - (reply: 1)
534. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
535. Dark western blot film - (reply: 12)
536. how to get red of high secondary signal - (reply: 4)
537. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
538. Detecting phosphoproteins - different results when samples rerun (reply: 1)
539. Is loading buffer ok? - (reply: 1)
540. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)

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