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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Western Blot no target bands, show Non-specific bands - (reply: 5)
512. how critical is the pH of tbst? - (reply: 2)
513. Fc fusion protein smearing on SDS PAGE - (reply: 2)
514. what do you recommend for Semi-Dry unit - (reply: 2)
515. Bands disappear after longer exposure - (reply: 4)
516. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
517. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
518. Antibody Optimization - (reply: 2)
519. Protein agregation in WB samples - (reply: 3)
520. Same Non-specific bands show up for different Abs!! - (reply: 7)
521. what caused bubble band of my WB? - (reply: 4)
522. blotting detection - (reply: 3)
523. Max amount of protein - (reply: 4)
524. Areas of membrane bleached - (reply: 1)
525. asking suggestions for western blot - (reply: 3)
526. Can i apply primary antibody for two nights instead of one? - (reply: 3)
527. Strange bands and large smear in western blot - (reply: 4)
528. Freezing proteins at -80 - (reply: 1)
529. Tris glycine SDS buffer prep pH error - (reply: 2)
530. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
531. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
532. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
533. problems with negative control - (reply: 2)
534. urgent-W.B visualisation on kodak film - (reply: 2)
535. NP 40 lysis buffer - (reply: 1)
536. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
537. Dark western blot film - (reply: 12)
538. how to get red of high secondary signal - (reply: 4)
539. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
540. Detecting phosphoproteins - different results when samples rerun (reply: 1)

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