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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Bradford reagent for staining gels? - (reply: 1)
512. developing film manually - (reply: 19)
513. non specific bands - (reply: 7)
514. BSA vs Milk - (reply: 2)
515. Western Blot no target bands, show Non-specific bands - (reply: 5)
516. how critical is the pH of tbst? - (reply: 2)
517. Fc fusion protein smearing on SDS PAGE - (reply: 2)
518. what do you recommend for Semi-Dry unit - (reply: 2)
519. Bands disappear after longer exposure - (reply: 4)
520. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
521. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
522. Antibody Optimization - (reply: 2)
523. Protein agregation in WB samples - (reply: 3)
524. Same Non-specific bands show up for different Abs!! - (reply: 7)
525. what caused bubble band of my WB? - (reply: 4)
526. blotting detection - (reply: 3)
527. Max amount of protein - (reply: 4)
528. Areas of membrane bleached - (reply: 1)
529. asking suggestions for western blot - (reply: 3)
530. Can i apply primary antibody for two nights instead of one? - (reply: 3)
531. Strange bands and large smear in western blot - (reply: 4)
532. Freezing proteins at -80 - (reply: 1)
533. Tris glycine SDS buffer prep pH error - (reply: 2)
534. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
535. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
536. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
537. problems with negative control - (reply: 2)
538. urgent-W.B visualisation on kodak film - (reply: 2)
539. NP 40 lysis buffer - (reply: 1)
540. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)

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