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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. Western blot not showing protein - (reply: 2)
512. normalisation of western blot from kodak film - (reply: 1)
513. antibody in 40% glycerol - (reply: 6)
514. longer running of proteins in SDS PAGE! - (reply: 2)
515. dirty back ground after exposure - (reply: 2)
516. Tenfold PBS in extraction - (reply: 3)
517. Separating close bands in Western Blot - (reply: 3)
518. Western blot on intestine samples - (reply: 2)
519. dot blot - (reply: 4)
520. ECL Prime Streamlining and Troubleshootin - (reply: 1)
521. Samples leaking out of wells in gel - (reply: 2)
522. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
523. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
524. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
525. Strange E.coli growth - (reply: 3)
526. Problem with Immunoprecipitated samples - (reply: 2)
527. problems in transfer - (reply: 3)
528. Phospho-JNK - (reply: 9)
529. Bradford reagent for staining gels? - (reply: 1)
530. developing film manually - (reply: 19)
531. non specific bands - (reply: 7)
532. BSA vs Milk - (reply: 2)
533. Western Blot no target bands, show Non-specific bands - (reply: 5)
534. how critical is the pH of tbst? - (reply: 2)
535. Fc fusion protein smearing on SDS PAGE - (reply: 2)
536. what do you recommend for Semi-Dry unit - (reply: 2)
537. Bands disappear after longer exposure - (reply: 4)
538. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
539. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
540. Antibody Optimization - (reply: 2)

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