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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
511. longer running of proteins in SDS PAGE! - (reply: 2)
512. dirty back ground after exposure - (reply: 2)
513. Tenfold PBS in extraction - (reply: 3)
514. Separating close bands in Western Blot - (reply: 3)
515. Western blot on intestine samples - (reply: 2)
516. dot blot - (reply: 4)
517. ECL Prime Streamlining and Troubleshootin - (reply: 1)
518. Samples leaking out of wells in gel - (reply: 2)
519. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
520. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
521. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
522. Strange E.coli growth - (reply: 3)
523. Problem with Immunoprecipitated samples - (reply: 2)
524. problems in transfer - (reply: 3)
525. Phospho-JNK - (reply: 9)
526. Bradford reagent for staining gels? - (reply: 1)
527. developing film manually - (reply: 19)
528. non specific bands - (reply: 7)
529. BSA vs Milk - (reply: 2)
530. Western Blot no target bands, show Non-specific bands - (reply: 5)
531. how critical is the pH of tbst? - (reply: 2)
532. Fc fusion protein smearing on SDS PAGE - (reply: 2)
533. what do you recommend for Semi-Dry unit - (reply: 2)
534. Bands disappear after longer exposure - (reply: 4)
535. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
536. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
537. Antibody Optimization - (reply: 2)
538. Protein agregation in WB samples - (reply: 3)
539. Same Non-specific bands show up for different Abs!! - (reply: 7)
540. what caused bubble band of my WB? - (reply: 4)

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