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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Centrifuge after lysing - (reply: 5)
782. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
783. "Diffuesed" bands of higher molecular weight - (reply: 6)
784. Dimeric form of a protein? - I want my monomer! (reply: 2)
785. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
786. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
787. how to get rabbit antibody for Western blotting - (reply: 2)
788. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
789. Not sure of what % to use for gels - (reply: 6)
790. Protein Size - (reply: 3)
791. no separation of MW marker on gel - (reply: 1)
792. Transfer basic protein to PVDF - (reply: 1)
793. Expired pre-cast gels - (reply: 4)
794. LDS sample buffer in 95C - (reply: 2)
795. disappearing high molecular protein and marker,thanks - (reply: 1)
796. Use of cold milk??? - Western blot (reply: 2)
797. Antibody compatibility between different species - (reply: 4)
798. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)
799. Wrong bands on the blot - (reply: 4)
800. Protein drag in IEF-WB - (reply: 3)
801. SDS-PAGE gel staining problem - (reply: 1)
802. sample buffer pH - its effect?? (reply: 12)
803. SDS-Page and DMSO - (reply: 2)
804. Help with Western Blotting of Keratin Proteins - (reply: 2)
805. Problems detecting my band! - (reply: 2)
806. How many times can I reuse Ponceau S? - (reply: 3)
807. sds-page problems - polymerization of acrylamide (reply: 5)
808. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
809. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
810. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)

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