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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Dimer or monomer - (reply: 11)
782. Protein precipitation by TCA acid + acetone - (reply: 4)
783. Non Reducing SDS protein ladder?? - (reply: 4)
784. Protein Free Blocking Buffers - (reply: 1)
785. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
786. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
787. SDS-Page issue - (reply: 5)
788. Fluorescent visualization of Western blot - (reply: 2)
789. MEthanol in NC membrane - (reply: 7)
790. Pre stained markers - (reply: 4)
791. Laminin 5 antibody - (reply: 1)
792. Molecular weight marker - got different pattern! (reply: 13)
793. SDS ladder - which type works better? (reply: 3)
794. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
795. the sensitive amount of protein for western blotting - (reply: 3)
796. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
797. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
798. How to determine final amount of 20 ng protein per WB well - (reply: 8)
799. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
800. nonreducing antibody in reducing condition - (reply: 1)
801. High Molecular weight protein separation - (reply: 2)
802. Secreted protein for loading control - (reply: 2)
803. Only borders stained in Silver!!! - (reply: 7)
804. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
805. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
806. bromophenol blue - (reply: 10)
807. Native Page transfer/detection problems - (reply: 1)
808. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
809. nuclear loading control - (reply: 2)
810. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)

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