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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Fermentas prestained protein marker issue! - (reply: 7)
782. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
783. Invitrogen NuPAGE Sample buffer - (reply: 1)
784. western exposure one part of band darker - (reply: 6)
785. non-specific bands - (reply: 5)
786. Crazy looking BN-PAGE gel - No, really. (reply: 5)
787. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
788. bands of protein seems like it did not move - (reply: 2)
789. downshifted band - (reply: 4)
790. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
791. Western Troubleshooting Help, please! - (reply: 3)
792. Re-use membrane in Western Blot - (reply: 3)
793. NP40 or Triton as membrane protein detergent - (reply: 2)
794. Dot blot- great, western blot - no signal - (reply: 3)
795. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
796. Problem in transfer high MW protein - (reply: 5)
797. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
798. dimerization or aggregation of my protein! - (reply: 3)
799. basic question in SDS and western blot - (reply: 1)
800. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
801. Transfer problems - (reply: 1)
802. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
803. Stripping temperature - have no 55C water bath (reply: 1)
804. Nonspecificity of anti-flag - (reply: 2)
805. Blocking buffer - (reply: 7)
806. Detection of phospho-proteins - (reply: 1)
807. light chain - (reply: 5)
808. ugly merged bands in western blot - (reply: 3)
809. Western Blot Data Analysis - (reply: 2)
810. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)

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