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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. How many times can I reuse Ponceau S? - (reply: 3)
782. sds-page problems - polymerization of acrylamide (reply: 5)
783. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
784. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
785. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
786. Interpreting a gel - (reply: 3)
787. Plant isoenzymes! - (reply: 7)
788. cytochrome C Western Blot - (reply: 7)
789. Protein size and gel percentage - (reply: 7)
790. Bands visible in membrane but not in the film - ?? (reply: 10)
791. non desired brigther bands - (reply: 7)
792. Dimer or monomer - (reply: 11)
793. Protein precipitation by TCA acid + acetone - (reply: 4)
794. Non Reducing SDS protein ladder?? - (reply: 4)
795. Protein Free Blocking Buffers - (reply: 1)
796. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
797. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
798. SDS-Page issue - (reply: 5)
799. Fluorescent visualization of Western blot - (reply: 2)
800. MEthanol in NC membrane - (reply: 7)
801. Pre stained markers - (reply: 4)
802. Laminin 5 antibody - (reply: 1)
803. Molecular weight marker - got different pattern! (reply: 13)
804. SDS ladder - which type works better? (reply: 3)
805. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
806. the sensitive amount of protein for western blotting - (reply: 3)
807. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
808. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
809. How to determine final amount of 20 ng protein per WB well - (reply: 8)
810. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)

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