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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
782. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
783. Interpreting a gel - (reply: 3)
784. Plant isoenzymes! - (reply: 7)
785. cytochrome C Western Blot - (reply: 7)
786. Protein size and gel percentage - (reply: 7)
787. Bands visible in membrane but not in the film - ?? (reply: 10)
788. non desired brigther bands - (reply: 7)
789. Dimer or monomer - (reply: 11)
790. Protein precipitation by TCA acid + acetone - (reply: 4)
791. Non Reducing SDS protein ladder?? - (reply: 4)
792. Protein Free Blocking Buffers - (reply: 1)
793. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
794. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
795. SDS-Page issue - (reply: 5)
796. Fluorescent visualization of Western blot - (reply: 2)
797. MEthanol in NC membrane - (reply: 7)
798. Pre stained markers - (reply: 4)
799. Laminin 5 antibody - (reply: 1)
800. Molecular weight marker - got different pattern! (reply: 13)
801. SDS ladder - which type works better? (reply: 3)
802. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
803. the sensitive amount of protein for western blotting - (reply: 3)
804. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
805. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
806. How to determine final amount of 20 ng protein per WB well - (reply: 8)
807. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
808. nonreducing antibody in reducing condition - (reply: 1)
809. High Molecular weight protein separation - (reply: 2)
810. Secreted protein for loading control - (reply: 2)

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