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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Re-use membrane in Western Blot - (reply: 3)
782. NP40 or Triton as membrane protein detergent - (reply: 2)
783. Dot blot- great, western blot - no signal - (reply: 3)
784. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
785. Problem in transfer high MW protein - (reply: 5)
786. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
787. dimerization or aggregation of my protein! - (reply: 3)
788. basic question in SDS and western blot - (reply: 1)
789. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
790. Transfer problems - (reply: 1)
791. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
792. Stripping temperature - have no 55C water bath (reply: 1)
793. Nonspecificity of anti-flag - (reply: 2)
794. Blocking buffer - (reply: 7)
795. Detection of phospho-proteins - (reply: 1)
796. light chain - (reply: 5)
797. ugly merged bands in western blot - (reply: 3)
798. Western Blot Data Analysis - (reply: 2)
799. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
800. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
801. Protein Expression in stationary phase - (reply: 11)
802. Protein Smearing - (reply: 2)
803. Western blot for muscle - (reply: 2)
804. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
805. Identify the band in western blot - (reply: 5)
806. What kind of post-translational modification do you think are these? - (reply: 2)
807. Need help with Caspase WB - (reply: 2)
808. to keep sds-pa gels - (reply: 2)
809. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
810. Ponceau staining - Appearence of bands (reply: 1)

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