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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
782. SEmi-dry transfer blotting - (reply: 1)
783. Whole cell lysis of fibroblasts - (reply: 1)
784. SDS-PAGE: Strange Running - (reply: 8)
785. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
786. Different TBST pH's for WB incubation, why? - (reply: 1)
787. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
788. stability of the stripping buffer - (reply: 2)
789. small proteins have run ahead of tracking dye - (reply: 1)
790. cytoplasmic and nuclear extraction - (reply: 2)
791. Equal Protein Quantity - Loading onto Well (reply: 6)
792. Help, odd pattern - pic included - (reply: 3)
793. Fermentas prestained protein marker issue! - (reply: 7)
794. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
795. Invitrogen NuPAGE Sample buffer - (reply: 1)
796. western exposure one part of band darker - (reply: 6)
797. non-specific bands - (reply: 5)
798. Crazy looking BN-PAGE gel - No, really. (reply: 5)
799. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
800. bands of protein seems like it did not move - (reply: 2)
801. downshifted band - (reply: 4)
802. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
803. Western Troubleshooting Help, please! - (reply: 3)
804. Re-use membrane in Western Blot - (reply: 3)
805. NP40 or Triton as membrane protein detergent - (reply: 2)
806. Dot blot- great, western blot - no signal - (reply: 3)
807. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
808. Problem in transfer high MW protein - (reply: 5)
809. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
810. dimerization or aggregation of my protein! - (reply: 3)

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