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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. western exposure one part of band darker - (reply: 6)
782. non-specific bands - (reply: 5)
783. Crazy looking BN-PAGE gel - No, really. (reply: 5)
784. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
785. bands of protein seems like it did not move - (reply: 2)
786. downshifted band - (reply: 4)
787. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
788. Western Troubleshooting Help, please! - (reply: 3)
789. Re-use membrane in Western Blot - (reply: 3)
790. NP40 or Triton as membrane protein detergent - (reply: 2)
791. Dot blot- great, western blot - no signal - (reply: 3)
792. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
793. Problem in transfer high MW protein - (reply: 5)
794. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
795. dimerization or aggregation of my protein! - (reply: 3)
796. basic question in SDS and western blot - (reply: 1)
797. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
798. Transfer problems - (reply: 1)
799. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
800. Stripping temperature - have no 55C water bath (reply: 1)
801. Nonspecificity of anti-flag - (reply: 2)
802. Blocking buffer - (reply: 7)
803. Detection of phospho-proteins - (reply: 1)
804. light chain - (reply: 5)
805. ugly merged bands in western blot - (reply: 3)
806. Western Blot Data Analysis - (reply: 2)
807. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
808. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
809. Protein Expression in stationary phase - (reply: 11)
810. Protein Smearing - (reply: 2)

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