Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Equal Protein Quantity - Loading onto Well (reply: 6)
782. Help, odd pattern - pic included - (reply: 3)
783. Fermentas prestained protein marker issue! - (reply: 7)
784. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
785. Invitrogen NuPAGE Sample buffer - (reply: 1)
786. western exposure one part of band darker - (reply: 6)
787. non-specific bands - (reply: 5)
788. Crazy looking BN-PAGE gel - No, really. (reply: 5)
789. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
790. bands of protein seems like it did not move - (reply: 2)
791. downshifted band - (reply: 4)
792. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
793. Western Troubleshooting Help, please! - (reply: 3)
794. Re-use membrane in Western Blot - (reply: 3)
795. NP40 or Triton as membrane protein detergent - (reply: 2)
796. Dot blot- great, western blot - no signal - (reply: 3)
797. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
798. Problem in transfer high MW protein - (reply: 5)
799. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
800. dimerization or aggregation of my protein! - (reply: 3)
801. basic question in SDS and western blot - (reply: 1)
802. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
803. Transfer problems - (reply: 1)
804. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
805. Stripping temperature - have no 55C water bath (reply: 1)
806. Nonspecificity of anti-flag - (reply: 2)
807. Blocking buffer - (reply: 7)
808. Detection of phospho-proteins - (reply: 1)
809. light chain - (reply: 5)
810. ugly merged bands in western blot - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37