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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
782. Different TBST pH's for WB incubation, why? - (reply: 1)
783. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
784. stability of the stripping buffer - (reply: 2)
785. small proteins have run ahead of tracking dye - (reply: 1)
786. cytoplasmic and nuclear extraction - (reply: 2)
787. Equal Protein Quantity - Loading onto Well (reply: 6)
788. Help, odd pattern - pic included - (reply: 3)
789. Fermentas prestained protein marker issue! - (reply: 7)
790. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
791. Invitrogen NuPAGE Sample buffer - (reply: 1)
792. western exposure one part of band darker - (reply: 6)
793. non-specific bands - (reply: 5)
794. Crazy looking BN-PAGE gel - No, really. (reply: 5)
795. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
796. bands of protein seems like it did not move - (reply: 2)
797. downshifted band - (reply: 4)
798. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
799. Western Troubleshooting Help, please! - (reply: 3)
800. Re-use membrane in Western Blot - (reply: 3)
801. NP40 or Triton as membrane protein detergent - (reply: 2)
802. Dot blot- great, western blot - no signal - (reply: 3)
803. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
804. Problem in transfer high MW protein - (reply: 5)
805. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
806. dimerization or aggregation of my protein! - (reply: 3)
807. basic question in SDS and western blot - (reply: 1)
808. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
809. Transfer problems - (reply: 1)
810. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)

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