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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
781. Protein concentration and - (reply: 3)
782. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
783. Multiple antibodies give no signal - (reply: 10)
784. Problem with protein samples - No bands detected at all on gel (reply: 2)
785. using cell lysate to run native gel? - (reply: 1)
786. Bradford Query - (reply: 5)
787. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
788. About GFP- tagged proteins in western blot - (reply: 1)
789. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
790. PVP-40 for blocking? - Instead of milk (reply: 2)
791. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
792. How to pase my Co-IP over the weekend? - (reply: 1)
793. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
794. Protein Sample Preparation - Western Bloting (reply: 1)
795. No signal at all - (reply: 16)
796. Centrifuge after lysing - (reply: 5)
797. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
798. "Diffuesed" bands of higher molecular weight - (reply: 6)
799. Dimeric form of a protein? - I want my monomer! (reply: 2)
800. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
801. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
802. how to get rabbit antibody for Western blotting - (reply: 2)
803. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
804. Not sure of what % to use for gels - (reply: 6)
805. Protein Size - (reply: 3)
806. no separation of MW marker on gel - (reply: 1)
807. Transfer basic protein to PVDF - (reply: 1)
808. Expired pre-cast gels - (reply: 4)
809. LDS sample buffer in 95C - (reply: 2)
810. disappearing high molecular protein and marker,thanks - (reply: 1)

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