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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Western Blot Wet Transfer Conditions - (reply: 10)
122. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
123. Western Blot and Inmunofluorescence contradictions - (reply: 12)
124. Nanog double band in WB - (reply: 1)
125. My beta actin bands are changing - (reply: 6)
126. ECL Substrate Choices - (reply: 2)
127. Why does my gel have streaks instead of bands? - (reply: 2)
128. Left ECL kit at room temp. Is it ruined? - (reply: 2)
129. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
130. Native PAGE problem - not entering gel enough - (reply: 9)
131. film saturation in immunoblots - (reply: 1)
132. Problems with wells on SDS PAGE gels - (reply: 3)
133. Protein is present, but not detected by antibody - (reply: 4)
134. secondary antibody creation - (reply: 1)
135. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
136. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
137. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
138. Low signal, weird background stains on blot - (reply: 5)
139. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
140. Incomplete Blotting and staining of NC membrane - (reply: 7)
141. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
142. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
143. Help, protein ladder didn`t separate - (reply: 3)
144. Determining phosphorylation status of a protein by transfection - (reply: 5)
145. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
146. Problem with Denative lysis - (reply: 1)
147. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
148. Polyclonal antibody precipitating - (reply: 1)
149. manual 2D electrophoresis - (reply: 8)
150. relationship between protein size, lysate concentration, and band intensity - (reply: 3)

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