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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
122. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
123. Help, protein ladder didn`t separate - (reply: 3)
124. Determining phosphorylation status of a protein by transfection - (reply: 5)
125. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
126. Problem with Denative lysis - (reply: 1)
127. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
128. Polyclonal antibody precipitating - (reply: 1)
129. manual 2D electrophoresis - (reply: 8)
130. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
131. sample sonication before loading - (reply: 6)
132. transfer of low molecular weight protein - (reply: 3)
133. How is the mass detected via SDS-PAGE? - (reply: 3)
134. Water in beads during boiling - (reply: 3)
135. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
136. Runing problems - (reply: 18)
137. Western Blotting of Amyloid Beta - (reply: 1)
138. 10x Laemmli buffer - (reply: 5)
139. Need help translating my western blot protocol into every language - (reply: 3)
140. Semi-dry system for NuPage with MES buffer - (reply: 4)
141. Recommend anti-rat HRP secondary antibody - (reply: 2)
142. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
143. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
144. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
145. Western blot to CD44? - (reply: 3)
146. Amount of EDTA - (reply: 4)
147. SDS Apparatus for northern - (reply: 1)
148. Prey protein band bleeds, IgG band normal? - (reply: 3)
149. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
150. His-Probe - (reply: 2)

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