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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
122. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
123. Protein amount to load for publication figure? - (reply: 4)
124. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
125. What is different between Native protein and Denatured protein samples. - (reply: 4)
126. 3 extra bands show up on my western after an IP - (reply: 2)
127. IgG heavy chain from IP - (reply: 8)
128. about western blot analysis - (reply: 1)
129. Western Blotting loading control for large proteins - (reply: 2)
130. Uneven beta actin - (reply: 2)
131. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
132. NCOR1 western - (reply: 8)
133. Discrepancy between WB and Flow cytometry result - (reply: 5)
134. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
135. Western blotting transfer - (reply: 2)
136. Western Quantification (No BCA???) - (reply: 2)
137. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
138. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
139. Trouble shooting in Far-western blot. - (reply: 1)
140. Transcription/Translation reaction not resolving - (reply: 7)
141. Ponceau red problem: membrane turned red - (reply: 3)
142. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
143. No bands/weird bands on western blot - (reply: 3)
144. Protein ladder not separating - (reply: 6)
145. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
146. Preparing supernatant from cell culture for western blot - (reply: 4)
147. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
148. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
149. Curved bands in Western Blot - (reply: 2)
150. Some bands not coming up, others uneven - (reply: 9)

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