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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. My beta actin bands are changing - (reply: 6)
122. ECL Substrate Choices - (reply: 2)
123. Why does my gel have streaks instead of bands? - (reply: 2)
124. Left ECL kit at room temp. Is it ruined? - (reply: 2)
125. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
126. Native PAGE problem - not entering gel enough - (reply: 9)
127. film saturation in immunoblots - (reply: 1)
128. Problems with wells on SDS PAGE gels - (reply: 3)
129. Protein is present, but not detected by antibody - (reply: 4)
130. secondary antibody creation - (reply: 1)
131. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
132. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
133. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
134. Low signal, weird background stains on blot - (reply: 5)
135. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
136. Incomplete Blotting and staining of NC membrane - (reply: 7)
137. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
138. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
139. Help, protein ladder didn`t separate - (reply: 3)
140. Determining phosphorylation status of a protein by transfection - (reply: 5)
141. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
142. Problem with Denative lysis - (reply: 1)
143. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
144. Polyclonal antibody precipitating - (reply: 1)
145. manual 2D electrophoresis - (reply: 8)
146. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
147. sample sonication before loading - (reply: 6)
148. transfer of low molecular weight protein - (reply: 3)
149. How is the mass detected via SDS-PAGE? - (reply: 3)
150. Water in beads during boiling - (reply: 3)

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