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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Ladder and proteins do not migrate into stacking gel - (reply: 5)
122. Western Blot: Unequal Sample Migration - (reply: 4)
123. Western blot with smeared bands - (reply: 3)
124. SDS PAGE sample preparation question, and discussion? - (reply: 3)
125. too much heating LDS-sample buffer - (reply: 3)
126. problem of cell lysate - (reply: 3)
127. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
128. Blocking agent in western blotting of histone proteins - (reply: 1)
129. Best western loading control for comparing different cell lines - (reply: 2)
130. western blotting running problem? - (reply: 2)
131. Keeping apoptotic/detached cells for WB? - (reply: 3)
132. Advice with WB please - (reply: 3)
133. Western Blot Wet Transfer Conditions - (reply: 10)
134. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
135. Western Blot and Inmunofluorescence contradictions - (reply: 12)
136. Nanog double band in WB - (reply: 1)
137. My beta actin bands are changing - (reply: 6)
138. ECL Substrate Choices - (reply: 2)
139. Why does my gel have streaks instead of bands? - (reply: 2)
140. Left ECL kit at room temp. Is it ruined? - (reply: 2)
141. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
142. Native PAGE problem - not entering gel enough - (reply: 9)
143. film saturation in immunoblots - (reply: 1)
144. Problems with wells on SDS PAGE gels - (reply: 3)
145. Protein is present, but not detected by antibody - (reply: 4)
146. secondary antibody creation - (reply: 1)
147. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
148. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
149. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
150. Low signal, weird background stains on blot - (reply: 5)

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