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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Primary antibody - (reply: 1)
122. Native-PAGE, help! - (reply: 3)
123. Trouble with purified recombinant protein bands - (reply: 3)
124. Stain in electrophoresis is going from + to - - (reply: 3)
125. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
126. Extraction of Transmembrane Proteins - (reply: 1)
127. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
128. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
129. Protein amount to load for publication figure? - (reply: 4)
130. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
131. What is different between Native protein and Denatured protein samples. - (reply: 4)
132. 3 extra bands show up on my western after an IP - (reply: 2)
133. IgG heavy chain from IP - (reply: 8)
134. about western blot analysis - (reply: 1)
135. Western Blotting loading control for large proteins - (reply: 2)
136. Uneven beta actin - (reply: 2)
137. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
138. NCOR1 western - (reply: 8)
139. Discrepancy between WB and Flow cytometry result - (reply: 5)
140. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
141. Western blotting transfer - (reply: 2)
142. Western Quantification (No BCA???) - (reply: 2)
143. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
144. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
145. Trouble shooting in Far-western blot. - (reply: 1)
146. Transcription/Translation reaction not resolving - (reply: 7)
147. Ponceau red problem: membrane turned red - (reply: 3)
148. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
149. No bands/weird bands on western blot - (reply: 3)
150. Protein ladder not separating - (reply: 6)

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