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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Problem with SDS-PAGE gel - (reply: 13)
122. How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
123. SDS-Page trouble-shooting - (reply: 3)
124. PVDF membrane long-term storage after transfer - (reply: 3)
125. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
126. Distorted bands from human colon samples - (reply: 2)
127. "Wavy" bands in SDS-PAGE gel - (reply: 5)
128. LMW proteins not transfer of gel to membrane - (reply: 1)
129. smear in SDS PAGE - (reply: 4)
130. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
131. Smeared Bands after Ponceau Staining? - (reply: 3)
132. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)
133. patchy/holey bands after transfer, invasive bubbles? - (reply: 5)
134. Western not working - (reply: 4)
135. fused bands of beta-actin in western blot - (reply: 2)
136. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
137. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
138. Problem with GFP detection in western - (reply: 6)
139. Sudden Trouble with PVDF Transfer - (reply: 6)
140. Equal Loading Confusion - (reply: 6)
141. Wrong protein size on western blot - (reply: 10)
142. How to know if the signal of western blot is saturated ? - (reply: 4)
143. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
144. 5x SDS lysis buffer - (reply: 5)
145. Protein measurement of Laemmli samples - (reply: 2)
146. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
147. Uneven banding - Western blot troubleshooting - (reply: 4)
148. Western Blot with fixed cells - (reply: 5)
149. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
150. Primary antibody - (reply: 1)

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