Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Native PAGE problem - not entering gel enough - (reply: 9)
122. film saturation in immunoblots - (reply: 1)
123. Problems with wells on SDS PAGE gels - (reply: 3)
124. Protein is present, but not detected by antibody - (reply: 4)
125. secondary antibody creation - (reply: 1)
126. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
127. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
128. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
129. Low signal, weird background stains on blot - (reply: 5)
130. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
131. Incomplete Blotting and staining of NC membrane - (reply: 7)
132. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
133. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
134. Help, protein ladder didn`t separate - (reply: 3)
135. Determining phosphorylation status of a protein by transfection - (reply: 5)
136. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
137. Problem with Denative lysis - (reply: 1)
138. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
139. Polyclonal antibody precipitating - (reply: 1)
140. manual 2D electrophoresis - (reply: 8)
141. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
142. sample sonication before loading - (reply: 6)
143. transfer of low molecular weight protein - (reply: 3)
144. How is the mass detected via SDS-PAGE? - (reply: 3)
145. Water in beads during boiling - (reply: 3)
146. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
147. Runing problems - (reply: 18)
148. Western Blotting of Amyloid Beta - (reply: 1)
149. 10x Laemmli buffer - (reply: 5)
150. Need help translating my western blot protocol into every language - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36