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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Western Blot and Inmunofluorescence contradictions - (reply: 12)
122. Nanog double band in WB - (reply: 1)
123. My beta actin bands are changing - (reply: 6)
124. ECL Substrate Choices - (reply: 2)
125. Why does my gel have streaks instead of bands? - (reply: 2)
126. Left ECL kit at room temp. Is it ruined? - (reply: 2)
127. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
128. Native PAGE problem - not entering gel enough - (reply: 9)
129. film saturation in immunoblots - (reply: 1)
130. Problems with wells on SDS PAGE gels - (reply: 3)
131. Protein is present, but not detected by antibody - (reply: 4)
132. secondary antibody creation - (reply: 1)
133. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
134. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
135. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
136. Low signal, weird background stains on blot - (reply: 5)
137. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
138. Incomplete Blotting and staining of NC membrane - (reply: 7)
139. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
140. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
141. Help, protein ladder didn`t separate - (reply: 3)
142. Determining phosphorylation status of a protein by transfection - (reply: 5)
143. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
144. Problem with Denative lysis - (reply: 1)
145. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
146. Polyclonal antibody precipitating - (reply: 1)
147. manual 2D electrophoresis - (reply: 8)
148. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
149. sample sonication before loading - (reply: 6)
150. transfer of low molecular weight protein - (reply: 3)

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