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121. TNF alpha and IL 1b Western Blot - (reply: 1)
122. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
123. SDS-PAGE trouble with protein concentration - (reply: 2)
124. Acrylamid - (reply: 5)
125. Detecting doublet bands in Western for eGFP control - (reply: 1)
126. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
127. Protein Size 50 kDa higher than expected - (reply: 3)
128. Acid urea gel electrophoresis (AUGE) - (reply: 7)
129. Phospho-protein vs total protein Western blot - (reply: 5)
130. storing the loaded gel before running it - (reply: 7)
131. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
132. Western blot trouble shooting - (reply: 3)
133. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
134. two bands on WB-alternative use of start codons? - (reply: 2)
135. TCA Precipitation to remove albumin - (reply: 6)
136. adjusting pH of 4x stacking buffer - (reply: 3)
137. No WB bands for H2AX at all...! - (reply: 10)
138. any tips for doing Fluorescence Western Blot? - (reply: 2)
139. Unknown silver/grey residue left after transfer. - (reply: 2)
140. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
141. Changes to housekeeping loading controls after drug treatment - (reply: 2)
142. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
143. Multiple bands on blot with one antibody - (reply: 1)
144. sedimentation of cytosolic proteins - (reply: 2)
145. Determining pI of a protein - (reply: 3)
146. Non-Reduced Western Blot - (reply: 5)
147. Incomplete transfer - (reply: 5)
148. can coommassie stained gel be western blot - (reply: 5)
149. Wet transfer: western blot - (reply: 3)
150. Reprobing a PVDF membrane which has gone dry - (reply: 1)
122. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
123. SDS-PAGE trouble with protein concentration - (reply: 2)
124. Acrylamid - (reply: 5)
125. Detecting doublet bands in Western for eGFP control - (reply: 1)
126. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
127. Protein Size 50 kDa higher than expected - (reply: 3)
128. Acid urea gel electrophoresis (AUGE) - (reply: 7)
129. Phospho-protein vs total protein Western blot - (reply: 5)
130. storing the loaded gel before running it - (reply: 7)
131. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
132. Western blot trouble shooting - (reply: 3)
133. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
134. two bands on WB-alternative use of start codons? - (reply: 2)
135. TCA Precipitation to remove albumin - (reply: 6)
136. adjusting pH of 4x stacking buffer - (reply: 3)
137. No WB bands for H2AX at all...! - (reply: 10)
138. any tips for doing Fluorescence Western Blot? - (reply: 2)
139. Unknown silver/grey residue left after transfer. - (reply: 2)
140. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
141. Changes to housekeeping loading controls after drug treatment - (reply: 2)
142. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
143. Multiple bands on blot with one antibody - (reply: 1)
144. sedimentation of cytosolic proteins - (reply: 2)
145. Determining pI of a protein - (reply: 3)
146. Non-Reduced Western Blot - (reply: 5)
147. Incomplete transfer - (reply: 5)
148. can coommassie stained gel be western blot - (reply: 5)
149. Wet transfer: western blot - (reply: 3)
150. Reprobing a PVDF membrane which has gone dry - (reply: 1)