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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
121. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
122. how can i explain the different pattern from my labmate's one - (reply: 8)
123. Ladder and proteins do not migrate into stacking gel - (reply: 5)
124. Western Blot: Unequal Sample Migration - (reply: 4)
125. Western blot with smeared bands - (reply: 3)
126. SDS PAGE sample preparation question, and discussion? - (reply: 3)
127. too much heating LDS-sample buffer - (reply: 3)
128. problem of cell lysate - (reply: 3)
129. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
130. Blocking agent in western blotting of histone proteins - (reply: 1)
131. Best western loading control for comparing different cell lines - (reply: 2)
132. western blotting running problem? - (reply: 2)
133. Keeping apoptotic/detached cells for WB? - (reply: 3)
134. Advice with WB please - (reply: 3)
135. Western Blot Wet Transfer Conditions - (reply: 10)
136. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
137. Western Blot and Inmunofluorescence contradictions - (reply: 12)
138. Nanog double band in WB - (reply: 1)
139. My beta actin bands are changing - (reply: 6)
140. ECL Substrate Choices - (reply: 2)
141. Why does my gel have streaks instead of bands? - (reply: 2)
142. Left ECL kit at room temp. Is it ruined? - (reply: 2)
143. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
144. Native PAGE problem - not entering gel enough - (reply: 9)
145. film saturation in immunoblots - (reply: 1)
146. Problems with wells on SDS PAGE gels - (reply: 3)
147. Protein is present, but not detected by antibody - (reply: 4)
148. secondary antibody creation - (reply: 1)
149. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
150. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)

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