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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
871. How many times can I reuse Ponceau S? - (reply: 3)
872. sds-page problems - polymerization of acrylamide (reply: 5)
873. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
874. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
875. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
876. Interpreting a gel - (reply: 3)
877. Plant isoenzymes! - (reply: 7)
878. cytochrome C Western Blot - (reply: 7)
879. Protein size and gel percentage - (reply: 7)
880. Bands visible in membrane but not in the film - ?? (reply: 10)
881. non desired brigther bands - (reply: 7)
882. Dimer or monomer - (reply: 11)
883. Protein precipitation by TCA acid + acetone - (reply: 4)
884. Non Reducing SDS protein ladder?? - (reply: 4)
885. Protein Free Blocking Buffers - (reply: 1)
886. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
887. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
888. SDS-Page issue - (reply: 5)
889. Fluorescent visualization of Western blot - (reply: 2)
890. MEthanol in NC membrane - (reply: 7)
891. Pre stained markers - (reply: 4)
892. Laminin 5 antibody - (reply: 1)
893. Molecular weight marker - got different pattern! (reply: 13)
894. SDS ladder - which type works better? (reply: 3)
895. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
896. the sensitive amount of protein for western blotting - (reply: 3)
897. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
898. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
899. How to determine final amount of 20 ng protein per WB well - (reply: 8)
900. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)

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