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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
871. sds-page problems - polymerization of acrylamide (reply: 5)
872. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
873. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
874. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
875. Interpreting a gel - (reply: 3)
876. Plant isoenzymes! - (reply: 7)
877. cytochrome C Western Blot - (reply: 7)
878. Protein size and gel percentage - (reply: 7)
879. Bands visible in membrane but not in the film - ?? (reply: 10)
880. non desired brigther bands - (reply: 7)
881. Dimer or monomer - (reply: 11)
882. Protein precipitation by TCA acid + acetone - (reply: 4)
883. Non Reducing SDS protein ladder?? - (reply: 4)
884. Protein Free Blocking Buffers - (reply: 1)
885. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
886. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
887. SDS-Page issue - (reply: 5)
888. Fluorescent visualization of Western blot - (reply: 2)
889. MEthanol in NC membrane - (reply: 7)
890. Pre stained markers - (reply: 4)
891. Laminin 5 antibody - (reply: 1)
892. Molecular weight marker - got different pattern! (reply: 13)
893. SDS ladder - which type works better? (reply: 3)
894. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
895. the sensitive amount of protein for western blotting - (reply: 3)
896. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
897. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
898. How to determine final amount of 20 ng protein per WB well - (reply: 8)
899. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
900. nonreducing antibody in reducing condition - (reply: 1)

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