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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
871. "Diffuesed" bands of higher molecular weight - (reply: 6)
872. Dimeric form of a protein? - I want my monomer! (reply: 2)
873. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
874. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
875. how to get rabbit antibody for Western blotting - (reply: 2)
876. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
877. Not sure of what % to use for gels - (reply: 6)
878. Protein Size - (reply: 3)
879. no separation of MW marker on gel - (reply: 1)
880. Transfer basic protein to PVDF - (reply: 1)
881. Expired pre-cast gels - (reply: 4)
882. LDS sample buffer in 95C - (reply: 2)
883. disappearing high molecular protein and marker,thanks - (reply: 1)
884. Use of cold milk??? - Western blot (reply: 2)
885. Antibody compatibility between different species - (reply: 4)
886. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)
887. Wrong bands on the blot - (reply: 4)
888. Protein drag in IEF-WB - (reply: 3)
889. SDS-PAGE gel staining problem - (reply: 1)
890. sample buffer pH - its effect?? (reply: 12)
891. SDS-Page and DMSO - (reply: 2)
892. Help with Western Blotting of Keratin Proteins - (reply: 2)
893. Problems detecting my band! - (reply: 2)
894. How many times can I reuse Ponceau S? - (reply: 3)
895. sds-page problems - polymerization of acrylamide (reply: 5)
896. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
897. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
898. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
899. Interpreting a gel - (reply: 3)
900. Plant isoenzymes! - (reply: 7)

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