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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
871. direct lysis in Laemmli buffer - (reply: 4)
872. anti-FLAG (M2) cross reaction - (reply: 6)
873. NFkB western blotting - (reply: 3)
874. Mannitol in Lysis Buffer - (reply: 1)
875. how long will a 1M DTT solution will last in -20C - (reply: 5)
876. pellet became insoluble after boiling. - (reply: 4)
877. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
878. Ladder but no protein - (reply: 4)
879. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
880. my membranes are blue! - silly mistake, what to do? (reply: 1)
881. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
882. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
883. Primary Antibody for SPDEF - (reply: 1)
884. Detecting Patterns of phosphorylated protein - (reply: 1)
885. Sample Diffusion Before Applying Current - (reply: 4)
886. IP and western blot - (reply: 5)
887. Semi-dry transfer conditions - Volts or Amps (reply: 1)
888. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
889. Destain after PoneauS - (reply: 6)
890. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
891. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
892. Variability in GAPDH - (reply: 5)
893. nitrocellulose or PVDF - (reply: 7)
894. crappy bands after developing - (reply: 3)
895. cloudy gel - (reply: 2)
896. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
897. Beginner Question - (reply: 6)
898. What's the problem with this SDS-PAGE? - (reply: 8)
899. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
900. Antibody Validation - Please help me! THis is very Urgent (reply: 2)

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