Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
871. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
872. what is the glycine for in running and trans buffer? - and what is the Tris for? (reply: 1)
873. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
874. Interpreting a gel - (reply: 3)
875. Plant isoenzymes! - (reply: 7)
876. cytochrome C Western Blot - (reply: 7)
877. Protein size and gel percentage - (reply: 7)
878. Bands visible in membrane but not in the film - ?? (reply: 10)
879. non desired brigther bands - (reply: 7)
880. Dimer or monomer - (reply: 11)
881. Protein precipitation by TCA acid + acetone - (reply: 4)
882. Non Reducing SDS protein ladder?? - (reply: 4)
883. Protein Free Blocking Buffers - (reply: 1)
884. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
885. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
886. SDS-Page issue - (reply: 5)
887. Fluorescent visualization of Western blot - (reply: 2)
888. MEthanol in NC membrane - (reply: 7)
889. Pre stained markers - (reply: 4)
890. Laminin 5 antibody - (reply: 1)
891. Molecular weight marker - got different pattern! (reply: 13)
892. SDS ladder - which type works better? (reply: 3)
893. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
894. the sensitive amount of protein for western blotting - (reply: 3)
895. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
896. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
897. How to determine final amount of 20 ng protein per WB well - (reply: 8)
898. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
899. nonreducing antibody in reducing condition - (reply: 1)
900. High Molecular weight protein separation - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36