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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Identically loaded western bands loose intensity from left to right - (reply: 3)
362. eIF4G western blot - (reply: 3)
363. smear bands in western blot - (reply: 12)
364. Huge background with HT22 cells phophoERK antibody - (reply: 3)
365. How to quantify the protein amount for serum samples ? - (reply: 1)
366. Huge background with HT22 cells phophoERK antibody - (reply: 1)
367. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
368. horizontal smear? - (reply: 2)
369. BSA versus milk in blocking and antibody incubation - (reply: 5)
370. Western blots are 'semi' quantitative? - (reply: 12)
371. bands fuse together - (reply: 1)
372. Phospho-protein detection - (reply: 2)
373. Help on using different antibodies in the same membrane - (reply: 8)
374. weird query for Western Blotting - (reply: 2)
375. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
376. Quick/One-step/automated Western Blot - (reply: 8)
377. Amount of antibody used in Western Blot - (reply: 1)
378. WB Transfer: Voltage vs. Current - (reply: 7)
379. Large protein blot ~400kDa - (reply: 3)
380. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
381. Stripping buffer for nitrocellulose membrane - (reply: 1)
382. PAGE for Separation of DNA - (reply: 3)
383. Western Blotting of Proteins less than 10 kDa - (reply: 1)
384. dot blot - (reply: 7)
385. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
386. Internal Standard for western blot - (reply: 1)
387. Sox-2 antibody detection using Western - (reply: 10)
388. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
389. gH2A.X blotting problem - (reply: 1)
390. Re-using membranes with ghost bands - (reply: 2)

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