Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Western blot band anaylisis - (reply: 3)
362. protein aggregation (?) in gelatin zymography - (reply: 1)
363. Use of wrong TBS concentration - (reply: 1)
364. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
365. SDS-PAGE staining for digestion assay - (reply: 1)
366. Can I improve my primary antibody? - (reply: 2)
367. got band in control positive but not in sample... - (reply: 2)
368. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
369. Problems with IPs - (reply: 2)
370. Band is at the wrong size, but same for all antibodies - (reply: 6)
371. Problems with western blot detection - (reply: 1)
372. Methods of concentrating total protein extracts - (reply: 2)
373. Using serum as the sample in Western blot - (reply: 3)
374. Puzzeling western blot results, missing band - (reply: 3)
375. Secondary AB blocking - (reply: 1)
376. western blood immunodetection Ab - (reply: 1)
377. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
378. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
379. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
380. What percentage should I use for a stacking gel? - (reply: 1)
381. DMSO - (reply: 1)
382. several questions on running SDS-PAGE - (reply: 1)
383. Voltage issues during transfer - (reply: 5)
384. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
385. What percentage gel should I make? - (reply: 1)
386. Western Blot problems-vertical streaking - (reply: 3)
387. Protein extraction from dead cells - (reply: 1)
388. Signal stuck on top of seperating gel - (reply: 1)
389. SDS-PAGE gel and running buffer - (reply: 3)
390. The same strong bands with different primary antibody - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36