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361. Wet transfer: western blot - (reply: 3)
362. Reprobing a PVDF membrane which has gone dry - (reply: 1)
363. Non-specific bands and fuzzy bands - (reply: 1)
364. protein showing up around 50 kda rather than 20 kda - (reply: 2)
365. Help for western blot wet transfer blurry bands - (reply: 2)
366. anti-BRCA1 antibody specificity issues - (reply: 2)
367. Concentrating a diluted protein and protein determination - (reply: 4)
368. Blurry bands ... - (reply: 12)
369. Dot blotting problems - (reply: 3)
370. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
371. Uneven lane widths during western blots - (reply: 10)
372. Using human serum as first antibody in Western/ELISA - (reply: 3)
373. Strip Western Blotting Troubleshooting - (reply: 2)
374. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
375. Phospho-ATF-2 coming up different sizes - (reply: 1)
376. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
377. A possible protocol for western blotting troubleshooting? - (reply: 1)
378. Vertical Vs horizontal electrophoresis - (reply: 3)
379. Reprobing with the same antibody - (reply: 3)
380. SDS-PAGE problem with staining yellow background - (reply: 3)
381. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
382. Western blot narrow bands - (reply: 1)
383. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
384. Western Blot Antibody Epitope - (reply: 1)
385. Western blot band anaylisis - (reply: 3)
386. protein aggregation (?) in gelatin zymography - (reply: 1)
387. Use of wrong TBS concentration - (reply: 1)
388. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
389. SDS-PAGE staining for digestion assay - (reply: 1)
390. Can I improve my primary antibody? - (reply: 2)
362. Reprobing a PVDF membrane which has gone dry - (reply: 1)
363. Non-specific bands and fuzzy bands - (reply: 1)
364. protein showing up around 50 kda rather than 20 kda - (reply: 2)
365. Help for western blot wet transfer blurry bands - (reply: 2)
366. anti-BRCA1 antibody specificity issues - (reply: 2)
367. Concentrating a diluted protein and protein determination - (reply: 4)
368. Blurry bands ... - (reply: 12)
369. Dot blotting problems - (reply: 3)
370. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
371. Uneven lane widths during western blots - (reply: 10)
372. Using human serum as first antibody in Western/ELISA - (reply: 3)
373. Strip Western Blotting Troubleshooting - (reply: 2)
374. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
375. Phospho-ATF-2 coming up different sizes - (reply: 1)
376. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
377. A possible protocol for western blotting troubleshooting? - (reply: 1)
378. Vertical Vs horizontal electrophoresis - (reply: 3)
379. Reprobing with the same antibody - (reply: 3)
380. SDS-PAGE problem with staining yellow background - (reply: 3)
381. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
382. Western blot narrow bands - (reply: 1)
383. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
384. Western Blot Antibody Epitope - (reply: 1)
385. Western blot band anaylisis - (reply: 3)
386. protein aggregation (?) in gelatin zymography - (reply: 1)
387. Use of wrong TBS concentration - (reply: 1)
388. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
389. SDS-PAGE staining for digestion assay - (reply: 1)
390. Can I improve my primary antibody? - (reply: 2)