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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Pull down assay and Co- IP experiment - (reply: 6)
362. Trouble detecting Histaq - (reply: 4)
363. Western gels running super slow! - (reply: 3)
364. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
365. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
366. WB parametric or non-parametric - (reply: 6)
367. Trouble with the GFP signal - (reply: 1)
368. Intern Needing Help - (reply: 3)
369. SDS-Electrophoresis, Dye color change - (reply: 1)
370. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
371. Trouble with equal loading - (reply: 8)
372. how many times striping can b done of the blot - (reply: 9)
373. SDS-PAGE of negatively charged protein - (reply: 4)
374. Protection Lanes? - (reply: 4)
375. Tie-2 westerns high background black lanes - (reply: 13)
376. BSA concentration for blocking - (reply: 5)
377. Identically loaded western bands loose intensity from left to right - (reply: 3)
378. eIF4G western blot - (reply: 3)
379. smear bands in western blot - (reply: 12)
380. Huge background with HT22 cells phophoERK antibody - (reply: 3)
381. How to quantify the protein amount for serum samples ? - (reply: 1)
382. Huge background with HT22 cells phophoERK antibody - (reply: 1)
383. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
384. horizontal smear? - (reply: 2)
385. BSA versus milk in blocking and antibody incubation - (reply: 5)
386. Western blots are 'semi' quantitative? - (reply: 12)
387. bands fuse together - (reply: 1)
388. Phospho-protein detection - (reply: 2)
389. Help on using different antibodies in the same membrane - (reply: 8)
390. weird query for Western Blotting - (reply: 2)

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