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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Use of wrong TBS concentration - (reply: 1)
362. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
363. SDS-PAGE staining for digestion assay - (reply: 1)
364. Can I improve my primary antibody? - (reply: 2)
365. got band in control positive but not in sample... - (reply: 2)
366. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
367. Problems with IPs - (reply: 2)
368. Band is at the wrong size, but same for all antibodies - (reply: 6)
369. Problems with western blot detection - (reply: 1)
370. Methods of concentrating total protein extracts - (reply: 2)
371. Using serum as the sample in Western blot - (reply: 3)
372. Puzzeling western blot results, missing band - (reply: 3)
373. Secondary AB blocking - (reply: 1)
374. western blood immunodetection Ab - (reply: 1)
375. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
376. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
377. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
378. What percentage should I use for a stacking gel? - (reply: 1)
379. DMSO - (reply: 1)
380. several questions on running SDS-PAGE - (reply: 1)
381. Voltage issues during transfer - (reply: 5)
382. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
383. What percentage gel should I make? - (reply: 1)
384. Western Blot problems-vertical streaking - (reply: 3)
385. Protein extraction from dead cells - (reply: 1)
386. Signal stuck on top of seperating gel - (reply: 1)
387. SDS-PAGE gel and running buffer - (reply: 3)
388. The same strong bands with different primary antibody - (reply: 1)
389. Trouble Western Blotting a 360kDa Protein - (reply: 5)
390. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)

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