Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Strip Western Blotting Troubleshooting - (reply: 2)
362. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
363. Phospho-ATF-2 coming up different sizes - (reply: 1)
364. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
365. A possible protocol for western blotting troubleshooting? - (reply: 1)
366. Vertical Vs horizontal electrophoresis - (reply: 3)
367. Reprobing with the same antibody - (reply: 3)
368. SDS-PAGE problem with staining yellow background - (reply: 3)
369. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
370. Western blot narrow bands - (reply: 1)
371. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
372. Western Blot Antibody Epitope - (reply: 1)
373. Western blot band anaylisis - (reply: 3)
374. protein aggregation (?) in gelatin zymography - (reply: 1)
375. Use of wrong TBS concentration - (reply: 1)
376. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
377. SDS-PAGE staining for digestion assay - (reply: 1)
378. Can I improve my primary antibody? - (reply: 2)
379. got band in control positive but not in sample... - (reply: 2)
380. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
381. Problems with IPs - (reply: 2)
382. Band is at the wrong size, but same for all antibodies - (reply: 6)
383. Problems with western blot detection - (reply: 1)
384. Methods of concentrating total protein extracts - (reply: 2)
385. Using serum as the sample in Western blot - (reply: 3)
386. Puzzeling western blot results, missing band - (reply: 3)
387. Secondary AB blocking - (reply: 1)
388. western blood immunodetection Ab - (reply: 1)
389. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
390. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37