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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Help for western blot wet transfer blurry bands - (reply: 2)
362. anti-BRCA1 antibody specificity issues - (reply: 2)
363. Concentrating a diluted protein and protein determination - (reply: 4)
364. Blurry bands ... - (reply: 12)
365. Dot blotting problems - (reply: 3)
366. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
367. Uneven lane widths during western blots - (reply: 10)
368. Using human serum as first antibody in Western/ELISA - (reply: 3)
369. Strip Western Blotting Troubleshooting - (reply: 2)
370. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
371. Phospho-ATF-2 coming up different sizes - (reply: 1)
372. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
373. A possible protocol for western blotting troubleshooting? - (reply: 1)
374. Vertical Vs horizontal electrophoresis - (reply: 3)
375. Reprobing with the same antibody - (reply: 3)
376. SDS-PAGE problem with staining yellow background - (reply: 3)
377. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
378. Western blot narrow bands - (reply: 1)
379. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
380. Western Blot Antibody Epitope - (reply: 1)
381. Western blot band anaylisis - (reply: 3)
382. protein aggregation (?) in gelatin zymography - (reply: 1)
383. Use of wrong TBS concentration - (reply: 1)
384. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
385. SDS-PAGE staining for digestion assay - (reply: 1)
386. Can I improve my primary antibody? - (reply: 2)
387. got band in control positive but not in sample... - (reply: 2)
388. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
389. Problems with IPs - (reply: 2)
390. Band is at the wrong size, but same for all antibodies - (reply: 6)

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