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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Incomplete transfer - (reply: 5)
362. can coommassie stained gel be western blot - (reply: 5)
363. Wet transfer: western blot - (reply: 3)
364. Reprobing a PVDF membrane which has gone dry - (reply: 1)
365. Non-specific bands and fuzzy bands - (reply: 1)
366. protein showing up around 50 kda rather than 20 kda - (reply: 2)
367. Help for western blot wet transfer blurry bands - (reply: 2)
368. anti-BRCA1 antibody specificity issues - (reply: 2)
369. Concentrating a diluted protein and protein determination - (reply: 4)
370. Blurry bands ... - (reply: 12)
371. Dot blotting problems - (reply: 3)
372. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
373. Uneven lane widths during western blots - (reply: 10)
374. Using human serum as first antibody in Western/ELISA - (reply: 3)
375. Strip Western Blotting Troubleshooting - (reply: 2)
376. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
377. Phospho-ATF-2 coming up different sizes - (reply: 1)
378. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
379. A possible protocol for western blotting troubleshooting? - (reply: 1)
380. Vertical Vs horizontal electrophoresis - (reply: 3)
381. Reprobing with the same antibody - (reply: 3)
382. SDS-PAGE problem with staining yellow background - (reply: 3)
383. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
384. Western blot narrow bands - (reply: 1)
385. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
386. Western Blot Antibody Epitope - (reply: 1)
387. Western blot band anaylisis - (reply: 3)
388. protein aggregation (?) in gelatin zymography - (reply: 1)
389. Use of wrong TBS concentration - (reply: 1)
390. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)

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