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361. Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
362. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
363. Converging lanes in Western - (reply: 8)
364. Incomplete transfer - (reply: 7)
365. Anyone has run a PAGE with reducing agents in-gel?? - (reply: 3)
366. Non reducing protein ladder? - (reply: 5)
367. When to wash and how many times? - (reply: 4)
368. SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)
369. no detection of proteins at 25KDa - (reply: 4)
370. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
371. Huge background in Western blot - Studying the histone modification (reply: 1)
372. Protein detection - western blot (reply: 3)
373. Tricine-SDS gels blotting problems - (reply: 4)
374. native PAGE gel - (reply: 2)
375. nonspecific bands on film - (reply: 3)
376. RIPA recipe with Bradford compatibility..., - (reply: 1)
377. stripping of Western blot? - how does it work (reply: 1)
378. Imaging western blot membrane - (reply: 2)
379. Poll: Time and voltage for wet transfer to PVDF - (reply: 6)
380. Fluorescent Western Blotting Help - (reply: 8)
381. Western didnot work should I strip blot if i want to detect same protein-Notch I - (reply: 1)
382. troublshooting WB - (reply: 1)
383. Factors interfereing with chemiluminescence - (reply: 3)
384. Wet transfer vs Dry transfer - which one is better?? (reply: 2)
385. not "that" 2D gel - (reply: 2)
386. SDS-PAGE with 500 ug protein - (reply: 3)
387. washing after blocking PVDF filter - (reply: 2)
388. western blot - substarate buffer recepie (reply: 2)
389. Saving lysate with laemmli in -20? - (reply: 5)
390. Cell Lysates from a Western Blog - (reply: 2)
362. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
363. Converging lanes in Western - (reply: 8)
364. Incomplete transfer - (reply: 7)
365. Anyone has run a PAGE with reducing agents in-gel?? - (reply: 3)
366. Non reducing protein ladder? - (reply: 5)
367. When to wash and how many times? - (reply: 4)
368. SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)
369. no detection of proteins at 25KDa - (reply: 4)
370. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
371. Huge background in Western blot - Studying the histone modification (reply: 1)
372. Protein detection - western blot (reply: 3)
373. Tricine-SDS gels blotting problems - (reply: 4)
374. native PAGE gel - (reply: 2)
375. nonspecific bands on film - (reply: 3)
376. RIPA recipe with Bradford compatibility..., - (reply: 1)
377. stripping of Western blot? - how does it work (reply: 1)
378. Imaging western blot membrane - (reply: 2)
379. Poll: Time and voltage for wet transfer to PVDF - (reply: 6)
380. Fluorescent Western Blotting Help - (reply: 8)
381. Western didnot work should I strip blot if i want to detect same protein-Notch I - (reply: 1)
382. troublshooting WB - (reply: 1)
383. Factors interfereing with chemiluminescence - (reply: 3)
384. Wet transfer vs Dry transfer - which one is better?? (reply: 2)
385. not "that" 2D gel - (reply: 2)
386. SDS-PAGE with 500 ug protein - (reply: 3)
387. washing after blocking PVDF filter - (reply: 2)
388. western blot - substarate buffer recepie (reply: 2)
389. Saving lysate with laemmli in -20? - (reply: 5)
390. Cell Lysates from a Western Blog - (reply: 2)