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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Tricine PAG - (reply: 1)
362. Simple Western = a western blot revolution? - (reply: 2)
363. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
364. Best phospho-ERK antibody - (reply: 3)
365. what are the best transfer conditions? - (reply: 4)
366. Cell Envelope Proteins Smeared - (reply: 1)
367. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
368. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
369. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
370. restaining of coommassie destained gel - (reply: 1)
371. Secondary antibody overnight? - (reply: 5)
372. Problems with protein estimation and western blots - (reply: 1)
373. Loading controls - (reply: 1)
374. Quantity of protein to charge - (reply: 1)
375. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
376. Tris-Hcl becomes cloudy. - (reply: 3)
377. Can FLAG IP, but cannot detect with Western - (reply: 4)
378. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
379. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
380. Native PAGE, interaction of proteins, validity - (reply: 4)
381. Smeared transfer on nitrocellulose membrane - (reply: 2)
382. blocking and primary antibody in different buffers? - (reply: 1)
383. Same non specific band for different target proteins - (reply: 1)
384. Types of 96-well plates used for BCA protein quantification - (reply: 1)
385. Dot blot halo - (reply: 4)
386. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
387. Western blot and isoelectric point - (reply: 1)
388. Western Blot Developing Cassette - (reply: 1)
389. Pull down assay and Co- IP experiment - (reply: 6)
390. Trouble detecting Histaq - (reply: 4)

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