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361. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
362. Uneven lane widths during western blots - (reply: 10)
363. Using human serum as first antibody in Western/ELISA - (reply: 3)
364. Strip Western Blotting Troubleshooting - (reply: 2)
365. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
366. Phospho-ATF-2 coming up different sizes - (reply: 1)
367. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
368. A possible protocol for western blotting troubleshooting? - (reply: 1)
369. Vertical Vs horizontal electrophoresis - (reply: 3)
370. Reprobing with the same antibody - (reply: 3)
371. SDS-PAGE problem with staining yellow background - (reply: 3)
372. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
373. Western blot narrow bands - (reply: 1)
374. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
375. Western Blot Antibody Epitope - (reply: 1)
376. Western blot band anaylisis - (reply: 3)
377. protein aggregation (?) in gelatin zymography - (reply: 1)
378. Use of wrong TBS concentration - (reply: 1)
379. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
380. SDS-PAGE staining for digestion assay - (reply: 1)
381. Can I improve my primary antibody? - (reply: 2)
382. got band in control positive but not in sample... - (reply: 2)
383. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
384. Problems with IPs - (reply: 2)
385. Band is at the wrong size, but same for all antibodies - (reply: 6)
386. Problems with western blot detection - (reply: 1)
387. Methods of concentrating total protein extracts - (reply: 2)
388. Using serum as the sample in Western blot - (reply: 3)
389. Puzzeling western blot results, missing band - (reply: 3)
390. Secondary AB blocking - (reply: 1)
362. Uneven lane widths during western blots - (reply: 10)
363. Using human serum as first antibody in Western/ELISA - (reply: 3)
364. Strip Western Blotting Troubleshooting - (reply: 2)
365. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
366. Phospho-ATF-2 coming up different sizes - (reply: 1)
367. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
368. A possible protocol for western blotting troubleshooting? - (reply: 1)
369. Vertical Vs horizontal electrophoresis - (reply: 3)
370. Reprobing with the same antibody - (reply: 3)
371. SDS-PAGE problem with staining yellow background - (reply: 3)
372. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
373. Western blot narrow bands - (reply: 1)
374. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
375. Western Blot Antibody Epitope - (reply: 1)
376. Western blot band anaylisis - (reply: 3)
377. protein aggregation (?) in gelatin zymography - (reply: 1)
378. Use of wrong TBS concentration - (reply: 1)
379. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
380. SDS-PAGE staining for digestion assay - (reply: 1)
381. Can I improve my primary antibody? - (reply: 2)
382. got band in control positive but not in sample... - (reply: 2)
383. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
384. Problems with IPs - (reply: 2)
385. Band is at the wrong size, but same for all antibodies - (reply: 6)
386. Problems with western blot detection - (reply: 1)
387. Methods of concentrating total protein extracts - (reply: 2)
388. Using serum as the sample in Western blot - (reply: 3)
389. Puzzeling western blot results, missing band - (reply: 3)
390. Secondary AB blocking - (reply: 1)