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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
362. Western blot narrow bands - (reply: 1)
363. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
364. Western Blot Antibody Epitope - (reply: 1)
365. Western blot band anaylisis - (reply: 3)
366. protein aggregation (?) in gelatin zymography - (reply: 1)
367. Use of wrong TBS concentration - (reply: 1)
368. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
369. SDS-PAGE staining for digestion assay - (reply: 1)
370. Can I improve my primary antibody? - (reply: 2)
371. got band in control positive but not in sample... - (reply: 2)
372. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
373. Problems with IPs - (reply: 2)
374. Band is at the wrong size, but same for all antibodies - (reply: 6)
375. Problems with western blot detection - (reply: 1)
376. Methods of concentrating total protein extracts - (reply: 2)
377. Using serum as the sample in Western blot - (reply: 3)
378. Puzzeling western blot results, missing band - (reply: 3)
379. Secondary AB blocking - (reply: 1)
380. western blood immunodetection Ab - (reply: 1)
381. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
382. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
383. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
384. What percentage should I use for a stacking gel? - (reply: 1)
385. DMSO - (reply: 1)
386. several questions on running SDS-PAGE - (reply: 1)
387. Voltage issues during transfer - (reply: 5)
388. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
389. What percentage gel should I make? - (reply: 1)
390. Western Blot problems-vertical streaking - (reply: 3)

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