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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Western Blot Developing Cassette - (reply: 1)
362. Pull down assay and Co- IP experiment - (reply: 6)
363. Trouble detecting Histaq - (reply: 4)
364. Western gels running super slow! - (reply: 3)
365. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
366. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
367. WB parametric or non-parametric - (reply: 6)
368. Trouble with the GFP signal - (reply: 1)
369. Intern Needing Help - (reply: 3)
370. SDS-Electrophoresis, Dye color change - (reply: 1)
371. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
372. Trouble with equal loading - (reply: 8)
373. how many times striping can b done of the blot - (reply: 9)
374. SDS-PAGE of negatively charged protein - (reply: 4)
375. Protection Lanes? - (reply: 4)
376. Tie-2 westerns high background black lanes - (reply: 13)
377. BSA concentration for blocking - (reply: 5)
378. Identically loaded western bands loose intensity from left to right - (reply: 3)
379. eIF4G western blot - (reply: 3)
380. smear bands in western blot - (reply: 12)
381. Huge background with HT22 cells phophoERK antibody - (reply: 3)
382. How to quantify the protein amount for serum samples ? - (reply: 1)
383. Huge background with HT22 cells phophoERK antibody - (reply: 1)
384. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
385. horizontal smear? - (reply: 2)
386. BSA versus milk in blocking and antibody incubation - (reply: 5)
387. Western blots are 'semi' quantitative? - (reply: 12)
388. bands fuse together - (reply: 1)
389. Phospho-protein detection - (reply: 2)
390. Help on using different antibodies in the same membrane - (reply: 8)

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