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SDS-PAGE-and-Western-Blotting
361.
2 primary antibodies, both binding actin...they shouldn't be :( -
(reply: 3)
362.
Uneven lane widths during western blots -
(reply: 10)
363.
Using human serum as first antibody in Western/ELISA -
(reply: 3)
364.
Strip Western Blotting Troubleshooting -
(reply: 2)
365.
Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? -
(reply: 3)
366.
Phospho-ATF-2 coming up different sizes -
(reply: 1)
367.
Fold-changes different when quantifying same protein via IF and Western -
(reply: 1)
368.
A possible protocol for western blotting troubleshooting? -
(reply: 1)
369.
Vertical Vs horizontal electrophoresis -
(reply: 3)
370.
Reprobing with the same antibody -
(reply: 3)
371.
SDS-PAGE problem with staining yellow background -
(reply: 3)
372.
SDS-PAGE for cell culture medium - sample preparation -
(reply: 1)
373.
Western blot narrow bands -
(reply: 1)
374.
Grainy image and no signal on Western using ChemiDoc -
(reply: 6)
375.
Western Blot Antibody Epitope -
(reply: 1)
376.
Western blot band anaylisis -
(reply: 3)
377.
protein aggregation (?) in gelatin zymography -
(reply: 1)
378.
Use of wrong TBS concentration -
(reply: 1)
379.
Transfer time optimisation - Overnight low voltage vs Few hours high voltage -
(reply: 1)
380.
SDS-PAGE staining for digestion assay -
(reply: 1)
381.
Can I improve my primary antibody? -
(reply: 2)
382.
got band in control positive but not in sample... -
(reply: 2)
383.
turboGFP Ab for EGFP detection in WB, posible? -
(reply: 1)
384.
Problems with IPs -
(reply: 2)
385.
Band is at the wrong size, but same for all antibodies -
(reply: 6)
386.
Problems with western blot detection -
(reply: 1)
387.
Methods of concentrating total protein extracts -
(reply: 2)
388.
Using serum as the sample in Western blot -
(reply: 3)
389.
Puzzeling western blot results, missing band -
(reply: 3)
390.
Secondary AB blocking -
(reply: 1)
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