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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. anti-BRCA1 antibody specificity issues - (reply: 2)
362. Concentrating a diluted protein and protein determination - (reply: 4)
363. Blurry bands ... - (reply: 12)
364. Dot blotting problems - (reply: 3)
365. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
366. Uneven lane widths during western blots - (reply: 10)
367. Using human serum as first antibody in Western/ELISA - (reply: 3)
368. Strip Western Blotting Troubleshooting - (reply: 2)
369. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
370. Phospho-ATF-2 coming up different sizes - (reply: 1)
371. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
372. A possible protocol for western blotting troubleshooting? - (reply: 1)
373. Vertical Vs horizontal electrophoresis - (reply: 3)
374. Reprobing with the same antibody - (reply: 3)
375. SDS-PAGE problem with staining yellow background - (reply: 3)
376. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
377. Western blot narrow bands - (reply: 1)
378. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
379. Western Blot Antibody Epitope - (reply: 1)
380. Western blot band anaylisis - (reply: 3)
381. protein aggregation (?) in gelatin zymography - (reply: 1)
382. Use of wrong TBS concentration - (reply: 1)
383. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
384. SDS-PAGE staining for digestion assay - (reply: 1)
385. Can I improve my primary antibody? - (reply: 2)
386. got band in control positive but not in sample... - (reply: 2)
387. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
388. Problems with IPs - (reply: 2)
389. Band is at the wrong size, but same for all antibodies - (reply: 6)
390. Problems with western blot detection - (reply: 1)

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