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361. Blurry bands ... - (reply: 12)
362. Dot blotting problems - (reply: 3)
363. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
364. Uneven lane widths during western blots - (reply: 10)
365. Using human serum as first antibody in Western/ELISA - (reply: 3)
366. Strip Western Blotting Troubleshooting - (reply: 2)
367. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
368. Phospho-ATF-2 coming up different sizes - (reply: 1)
369. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
370. A possible protocol for western blotting troubleshooting? - (reply: 1)
371. Vertical Vs horizontal electrophoresis - (reply: 3)
372. Reprobing with the same antibody - (reply: 3)
373. SDS-PAGE problem with staining yellow background - (reply: 3)
374. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
375. Western blot narrow bands - (reply: 1)
376. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
377. Western Blot Antibody Epitope - (reply: 1)
378. Western blot band anaylisis - (reply: 3)
379. protein aggregation (?) in gelatin zymography - (reply: 1)
380. Use of wrong TBS concentration - (reply: 1)
381. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
382. SDS-PAGE staining for digestion assay - (reply: 1)
383. Can I improve my primary antibody? - (reply: 2)
384. got band in control positive but not in sample... - (reply: 2)
385. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
386. Problems with IPs - (reply: 2)
387. Band is at the wrong size, but same for all antibodies - (reply: 6)
388. Problems with western blot detection - (reply: 1)
389. Methods of concentrating total protein extracts - (reply: 2)
390. Using serum as the sample in Western blot - (reply: 3)
362. Dot blotting problems - (reply: 3)
363. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
364. Uneven lane widths during western blots - (reply: 10)
365. Using human serum as first antibody in Western/ELISA - (reply: 3)
366. Strip Western Blotting Troubleshooting - (reply: 2)
367. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
368. Phospho-ATF-2 coming up different sizes - (reply: 1)
369. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
370. A possible protocol for western blotting troubleshooting? - (reply: 1)
371. Vertical Vs horizontal electrophoresis - (reply: 3)
372. Reprobing with the same antibody - (reply: 3)
373. SDS-PAGE problem with staining yellow background - (reply: 3)
374. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
375. Western blot narrow bands - (reply: 1)
376. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
377. Western Blot Antibody Epitope - (reply: 1)
378. Western blot band anaylisis - (reply: 3)
379. protein aggregation (?) in gelatin zymography - (reply: 1)
380. Use of wrong TBS concentration - (reply: 1)
381. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
382. SDS-PAGE staining for digestion assay - (reply: 1)
383. Can I improve my primary antibody? - (reply: 2)
384. got band in control positive but not in sample... - (reply: 2)
385. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
386. Problems with IPs - (reply: 2)
387. Band is at the wrong size, but same for all antibodies - (reply: 6)
388. Problems with western blot detection - (reply: 1)
389. Methods of concentrating total protein extracts - (reply: 2)
390. Using serum as the sample in Western blot - (reply: 3)