Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Determining pI of a protein - (reply: 3)
362. Non-Reduced Western Blot - (reply: 5)
363. Incomplete transfer - (reply: 5)
364. can coommassie stained gel be western blot - (reply: 5)
365. Wet transfer: western blot - (reply: 3)
366. Reprobing a PVDF membrane which has gone dry - (reply: 1)
367. Non-specific bands and fuzzy bands - (reply: 1)
368. protein showing up around 50 kda rather than 20 kda - (reply: 2)
369. Help for western blot wet transfer blurry bands - (reply: 2)
370. anti-BRCA1 antibody specificity issues - (reply: 2)
371. Concentrating a diluted protein and protein determination - (reply: 4)
372. Blurry bands ... - (reply: 12)
373. Dot blotting problems - (reply: 3)
374. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
375. Uneven lane widths during western blots - (reply: 10)
376. Using human serum as first antibody in Western/ELISA - (reply: 3)
377. Strip Western Blotting Troubleshooting - (reply: 2)
378. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
379. Phospho-ATF-2 coming up different sizes - (reply: 1)
380. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
381. A possible protocol for western blotting troubleshooting? - (reply: 1)
382. Vertical Vs horizontal electrophoresis - (reply: 3)
383. Reprobing with the same antibody - (reply: 3)
384. SDS-PAGE problem with staining yellow background - (reply: 3)
385. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
386. Western blot narrow bands - (reply: 1)
387. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
388. Western Blot Antibody Epitope - (reply: 1)
389. Western blot band anaylisis - (reply: 3)
390. protein aggregation (?) in gelatin zymography - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37