Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
361. Reprobing a PVDF membrane which has gone dry - (reply: 1)
362. Non-specific bands and fuzzy bands - (reply: 1)
363. protein showing up around 50 kda rather than 20 kda - (reply: 2)
364. Help for western blot wet transfer blurry bands - (reply: 2)
365. anti-BRCA1 antibody specificity issues - (reply: 2)
366. Concentrating a diluted protein and protein determination - (reply: 4)
367. Blurry bands ... - (reply: 12)
368. Dot blotting problems - (reply: 3)
369. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
370. Uneven lane widths during western blots - (reply: 10)
371. Using human serum as first antibody in Western/ELISA - (reply: 3)
372. Strip Western Blotting Troubleshooting - (reply: 2)
373. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
374. Phospho-ATF-2 coming up different sizes - (reply: 1)
375. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
376. A possible protocol for western blotting troubleshooting? - (reply: 1)
377. Vertical Vs horizontal electrophoresis - (reply: 3)
378. Reprobing with the same antibody - (reply: 3)
379. SDS-PAGE problem with staining yellow background - (reply: 3)
380. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
381. Western blot narrow bands - (reply: 1)
382. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
383. Western Blot Antibody Epitope - (reply: 1)
384. Western blot band anaylisis - (reply: 3)
385. protein aggregation (?) in gelatin zymography - (reply: 1)
386. Use of wrong TBS concentration - (reply: 1)
387. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
388. SDS-PAGE staining for digestion assay - (reply: 1)
389. Can I improve my primary antibody? - (reply: 2)
390. got band in control positive but not in sample... - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37