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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Storing membrane after antibody incubation - (reply: 2)
2. layer in between resolving and stacking gel - (reply: 1)
3. Lane spillover (?) on Western Blot - (reply: 1)
4. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
5. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
6. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
7. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
8. No band detected in WB - (reply: 4)
9. Concentration of Sample Buffer to boil protein samples - (reply: 2)
10. Western Blot diffuse bands - (reply: 3)
11. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
12. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
13. Some bands transferred, some not..? Why??? - (reply: 1)
14. Digital imager questions on correction - (reply: 4)
15. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
16. Rapidly decomposing HRP substrate - (reply: 2)
17. protein size in SDS-PAGE - (reply: 4)
18. Protein not migrating through gel - (reply: 9)
19. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
20. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
21. Extraction of bands from SDS-PAGE - (reply: 2)
22. Dumbbell-shaped bands on the WB - (reply: 3)
23. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
24. Deformed bands in gel - (reply: 8)
25. non specific immunoblot - (reply: 2)
26. Protein stuck in the origin of resolving gel - (reply: 12)
27. detection of small peptide by the SDS-PAGE gel - (reply: 1)
28. Help in western blotting GAPDH bands are not even - (reply: 1)
29. 37kb and 7kb protein in western blot - (reply: 24)
30. Dot blot/Western background signal problem - (reply: 1)

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