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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Biorad Miniprotean review - (reply: 1)
2. SDS PAGE gel length effect on resolution - (reply: 1)
3. 2D Blots problems - (reply: 6)
4. phospho-creb western blotting - (reply: 3)
5. Unspecific binding of multiple antibodies - (reply: 3)
6. gelatin zymogram - (reply: 1)
7. No or Super Strong Signal - (reply: 4)
8. Blotchy Blots - (reply: 2)
9. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
10. Dirty blots/high background - (reply: 5)
11. Heating to denature and reduce protein: same temperature? - (reply: 1)
12. Neurotoxicity of acrylamide - (reply: 2)
13. 2 bands very close in the expected place - (reply: 2)
14. Pressing Too Hard on Transfer Sandwich - (reply: 3)
15. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
16. SDS-PAGE High MW bands dissapear! - (reply: 7)
17. Non-specific binding of protein in western blot - (reply: 3)
18. SDS-PAGE vertical lane streaking - (reply: 1)
19. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
20. Storing membrane after antibody incubation - (reply: 2)
21. layer in between resolving and stacking gel - (reply: 1)
22. Lane spillover (?) on Western Blot - (reply: 3)
23. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
24. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
25. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
26. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
27. No band detected in WB - (reply: 4)
28. Concentration of Sample Buffer to boil protein samples - (reply: 2)
29. Western Blot diffuse bands - (reply: 3)
30. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)

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