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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
2. Extraction of bands from SDS-PAGE - (reply: 2)
3. Dumbbell-shaped bands on the WB - (reply: 1)
4. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
5. Deformed bands in gel - (reply: 8)
6. non specific immunoblot - (reply: 2)
7. Protein stuck in the origin of resolving gel - (reply: 12)
8. detection of small peptide by the SDS-PAGE gel - (reply: 1)
9. Help in western blotting GAPDH bands are not even - (reply: 1)
10. 37kb and 7kb protein in western blot - (reply: 24)
11. Dot blot/Western background signal problem - (reply: 1)
12. Blocking and Washing membrane - (reply: 3)
13. detection of a protein band of protein complex - (reply: 3)
14. Cell lysis - (reply: 1)
15. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
16. Western blot detects exogenous protein but not endogenous - (reply: 2)
17. White bands in western blotting - (reply: 4)
18. Question with the molecular weight of my protein - (reply: 1)
19. Possible Protein Aggregation Riddle - (reply: 3)
20. How to obtain a control positive for my WB - (reply: 3)
21. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
22. Antibody not binding to low MW targets - (reply: 4)
23. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
24. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
25. Question on loss of signal between samples in western blot - (reply: 4)
26. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
27. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
28. Antibody Issues - (reply: 6)
29. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
30. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)

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