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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
2. Western blot detects exogenous protein but not endogenous - (reply: 2)
3. White bands in western blotting - (reply: 3)
4. Question with the molecular weight of my protein - (reply: 1)
5. Possible Protein Aggregation Riddle - (reply: 3)
6. How to obtain a control positive for my WB - (reply: 3)
7. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
8. Antibody not binding to low MW targets - (reply: 4)
9. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
10. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
11. Question on loss of signal between samples in western blot - (reply: 4)
12. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
13. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
14. Antibody Issues - (reply: 6)
15. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
16. Antibody-Biotinylation, detection via SDS-Gel - (reply: 11)
17. Is it possible to "over-block" a membrane? - (reply: 5)
18. Troubleshooting for BN PAGE - (reply: 6)
19. SDS PAGE Destaining - (reply: 7)
20. Layer of water above separating gel mixes into gel - (reply: 2)
21. PCR & Western Blot sample preperation - (reply: 2)
22. SDS page - (reply: 8)
23. Two-night primary antibody incubation? - (reply: 2)
24. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
25. A question about Gelanalyzer - (reply: 8)
26. storing membrane - (reply: 2)
27. band obove the ladder - (reply: 3)
28. DNA SDS-PAGE electrophoresis - (reply: 6)
29. Axin1 Co-IP - (reply: 2)
30. Glycine vs. tricine - (reply: 1)

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