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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. phospho-creb western blotting - (reply: 3)
2. Unspecific binding of multiple antibodies - (reply: 3)
3. gelatin zymogram - (reply: 1)
4. No or Super Strong Signal - (reply: 4)
5. Blotchy Blots - (reply: 2)
6. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
7. Dirty blots/high background - (reply: 5)
8. Heating to denature and reduce protein: same temperature? - (reply: 1)
9. Neurotoxicity of acrylamide - (reply: 2)
10. 2 bands very close in the expected place - (reply: 2)
11. Pressing Too Hard on Transfer Sandwich - (reply: 3)
12. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
13. SDS-PAGE High MW bands dissapear! - (reply: 7)
14. Non-specific binding of protein in western blot - (reply: 3)
15. SDS-PAGE vertical lane streaking - (reply: 1)
16. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
17. Storing membrane after antibody incubation - (reply: 2)
18. layer in between resolving and stacking gel - (reply: 1)
19. Lane spillover (?) on Western Blot - (reply: 3)
20. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
21. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
22. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
23. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
24. No band detected in WB - (reply: 4)
25. Concentration of Sample Buffer to boil protein samples - (reply: 2)
26. Western Blot diffuse bands - (reply: 3)
27. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
28. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
29. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
30. Some bands transferred, some not..? Why??? - (reply: 1)

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