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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. ECL Substrate Choices - (reply: 2)
2. Why does my gel have streaks instead of bands? - (reply: 1)
3. Left ECL kit at room temp. Is it ruined? - (reply: 1)
4. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
5. Native PAGE problem - not entering gel enough - (reply: 5)
6. film saturation in immunoblots - (reply: 1)
7. Problems with wells on SDS PAGE gels - (reply: 3)
8. Protein is present, but not detected by antibody - (reply: 3)
9. secondary antibody creation - (reply: 1)
10. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
11. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
12. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
13. Low signal, weird background stains on blot - (reply: 5)
14. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
15. Incomplete Blotting and staining of NC membrane - (reply: 7)
16. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
17. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
18. Help, protein ladder didn`t separate - (reply: 3)
19. Determining phosphorylation status of a protein by transfection - (reply: 5)
20. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
21. Problem with Denative lysis - (reply: 1)
22. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
23. Polyclonal antibody precipitating - (reply: 1)
24. manual 2D electrophoresis - (reply: 8)
25. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
26. sample sonication before loading - (reply: 6)
27. transfer of low molecular weight protein - (reply: 3)
28. How is the mass detected via SDS-PAGE? - (reply: 3)
29. Water in beads during boiling - (reply: 3)
30. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)

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