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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. No or Super Strong Signal - (reply: 3)
2. Blotchy Blots - (reply: 2)
3. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
4. Dirty blots/high background - (reply: 4)
5. Heating to denature and reduce protein: same temperature? - (reply: 1)
6. Neurotoxicity of acrylamide - (reply: 2)
7. 2 bands very close in the expected place - (reply: 2)
8. Pressing Too Hard on Transfer Sandwich - (reply: 2)
9. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
10. SDS-PAGE High MW bands dissapear! - (reply: 6)
11. Non-specific binding of protein in western blot - (reply: 2)
12. SDS-PAGE vertical lane streaking - (reply: 1)
13. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
14. Storing membrane after antibody incubation - (reply: 2)
15. layer in between resolving and stacking gel - (reply: 1)
16. Lane spillover (?) on Western Blot - (reply: 3)
17. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
18. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
19. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
20. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
21. No band detected in WB - (reply: 4)
22. Concentration of Sample Buffer to boil protein samples - (reply: 2)
23. Western Blot diffuse bands - (reply: 3)
24. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
25. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
26. Some bands transferred, some not..? Why??? - (reply: 1)
27. Digital imager questions on correction - (reply: 4)
28. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
29. Rapidly decomposing HRP substrate - (reply: 2)
30. protein size in SDS-PAGE - (reply: 4)

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