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1. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
2. Protein amount to load for publication figure? - (reply: 4)
3. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
4. What is different between Native protein and Denatured protein samples. - (reply: 4)
5. 3 extra bands show up on my western after an IP - (reply: 2)
6. IgG heavy chain from IP - (reply: 1)
7. Western Blotting loading control for large proteins - (reply: 2)
8. Uneven beta actin - (reply: 2)
9. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
10. NCOR1 western - (reply: 8)
11. Discrepancy between WB and Flow cytometry result - (reply: 5)
12. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
13. Western blotting transfer - (reply: 2)
14. Western Quantification (No BCA???) - (reply: 2)
15. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
16. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
17. Trouble shooting in Far-western blot. - (reply: 1)
18. Transcription/Translation reaction not resolving - (reply: 6)
19. Ponceau red problem: membrane turned red - (reply: 3)
20. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
21. No bands/weird bands on western blot - (reply: 3)
22. Protein ladder not separating - (reply: 6)
23. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
24. Preparing supernatant from cell culture for western blot - (reply: 4)
25. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
26. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
27. Curved bands in Western Blot - (reply: 2)
28. Some bands not coming up, others uneven - (reply: 9)
29. Western blot and Triton X-100 in blocking buffer - (reply: 1)
30. Dot blot translation to WB - (reply: 2)
2. Protein amount to load for publication figure? - (reply: 4)
3. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
4. What is different between Native protein and Denatured protein samples. - (reply: 4)
5. 3 extra bands show up on my western after an IP - (reply: 2)
6. IgG heavy chain from IP - (reply: 1)
7. Western Blotting loading control for large proteins - (reply: 2)
8. Uneven beta actin - (reply: 2)
9. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
10. NCOR1 western - (reply: 8)
11. Discrepancy between WB and Flow cytometry result - (reply: 5)
12. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
13. Western blotting transfer - (reply: 2)
14. Western Quantification (No BCA???) - (reply: 2)
15. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
16. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
17. Trouble shooting in Far-western blot. - (reply: 1)
18. Transcription/Translation reaction not resolving - (reply: 6)
19. Ponceau red problem: membrane turned red - (reply: 3)
20. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
21. No bands/weird bands on western blot - (reply: 3)
22. Protein ladder not separating - (reply: 6)
23. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
24. Preparing supernatant from cell culture for western blot - (reply: 4)
25. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
26. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
27. Curved bands in Western Blot - (reply: 2)
28. Some bands not coming up, others uneven - (reply: 9)
29. Western blot and Triton X-100 in blocking buffer - (reply: 1)
30. Dot blot translation to WB - (reply: 2)