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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Western blot with smeared bands - (reply: 3)
2. SDS PAGE sample preparation question, and discussion? - (reply: 3)
3. too much heating LDS-sample buffer - (reply: 3)
4. problem of cell lysate - (reply: 3)
5. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
6. Blocking agent in western blotting of histone proteins - (reply: 1)
7. Best western loading control for comparing different cell lines - (reply: 2)
8. western blotting running problem? - (reply: 2)
9. Keeping apoptotic/detached cells for WB? - (reply: 3)
10. Advice with WB please - (reply: 3)
11. Western Blot Wet Transfer Conditions - (reply: 10)
12. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
13. Western Blot and Inmunofluorescence contradictions - (reply: 12)
14. Nanog double band in WB - (reply: 1)
15. My beta actin bands are changing - (reply: 6)
16. ECL Substrate Choices - (reply: 2)
17. Why does my gel have streaks instead of bands? - (reply: 2)
18. Left ECL kit at room temp. Is it ruined? - (reply: 2)
19. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
20. Native PAGE problem - not entering gel enough - (reply: 5)
21. film saturation in immunoblots - (reply: 1)
22. Problems with wells on SDS PAGE gels - (reply: 3)
23. Protein is present, but not detected by antibody - (reply: 4)
24. secondary antibody creation - (reply: 1)
25. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
26. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
27. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
28. Low signal, weird background stains on blot - (reply: 5)
29. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
30. Incomplete Blotting and staining of NC membrane - (reply: 7)

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