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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. Protein lysate storage - (reply: 2)
632. Is there alpha-tubulin in the nucleus? - (reply: 4)
633. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
634. No band after WB revelation - (reply: 2)
635. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
636. Expression by ICC but not by Western-why? - (reply: 3)
637. Re-Imaging without Stripping - (reply: 4)
638. MES buffer turned yellow - (reply: 5)
639. western blot woes - (reply: 2)
640. Size larger than expected - totally boggled - (reply: 2)
641. Ponceau Red Staining Problem - (reply: 2)
642. SDS-PAGE Second interface - (reply: 3)
643. biorad's 30% bis/acrylamide storage - (reply: 2)
644. protein concentration per well - (reply: 6)
645. dot blot - Problem (reply: 1)
646. Clear native PAGE - (reply: 1)
647. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
648. Weak western signal for help - (reply: 13)
649. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
650. Western not working anymore - (reply: 15)
651. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
652. BN-PAGE possible with Tris-glycine - (reply: 1)
653. Western few questions - (reply: 1)
654. thiourea in 2D page - What's it for? (reply: 1)
655. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
656. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
657. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
658. Antibody works in immunofluorescence, but not in WB - (reply: 4)
659. B-actin as western blot -control - (reply: 4)
660. horrific western blots , please help - (reply: 3)

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