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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
632. Lower MW bands than expected. - (reply: 1)
633. Question about reducing agent (DTT) - (reply: 1)
634. Collagen II western blotting - (reply: 1)
635. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
636. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
637. NUCLEAR PROTEIN PREPARATION - (reply: 1)
638. Lost protein from blot? - (reply: 1)
639. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
640. No SDS in gels - (reply: 12)
641. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
642. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
643. Sample loading buffer turning yellow. - (reply: 3)
644. western bands look funny - (reply: 5)
645. IgG SDS-PAGE - (reply: 1)
646. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
647. wet or semi-dry transfer for low MW proteins - (reply: 4)
648. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
649. quantifying "ghost" bands - (reply: 1)
650. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
651. Western blot running and loading samples - (reply: 6)
652. problems with His tagged protein western blots - (reply: 3)
653. Springerlink--Zymography - (reply: 3)
654. Methanol vs Isopropanol in fixing solution - (reply: 1)
655. Protein aggregates SDS-PAGE - (reply: 5)
656. probably DNA is disturbing the quantification of my sample - (reply: 3)
657. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
658. Optimising antibody concentration - hints and tips (reply: 1)
659. Lanes all run together - (reply: 5)
660. Protein Markers - (reply: 1)

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