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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
632. No SDS in gels - (reply: 12)
633. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
634. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
635. Sample loading buffer turning yellow. - (reply: 3)
636. western bands look funny - (reply: 5)
637. IgG SDS-PAGE - (reply: 1)
638. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
639. wet or semi-dry transfer for low MW proteins - (reply: 4)
640. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
641. quantifying "ghost" bands - (reply: 1)
642. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
643. Western blot running and loading samples - (reply: 6)
644. problems with His tagged protein western blots - (reply: 3)
645. Springerlink--Zymography - (reply: 3)
646. Methanol vs Isopropanol in fixing solution - (reply: 1)
647. Protein aggregates SDS-PAGE - (reply: 5)
648. probably DNA is disturbing the quantification of my sample - (reply: 3)
649. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
650. Optimising antibody concentration - hints and tips (reply: 1)
651. Lanes all run together - (reply: 5)
652. Protein Markers - (reply: 1)
653. Gel Running Crooked?? Please help.. - (reply: 7)
654. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
655. Strength of actin signal - How dark should the band be? (reply: 5)
656. Immunoprecipitation - (reply: 2)
657. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
658. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
659. SEmi-dry transfer blotting - (reply: 1)
660. Whole cell lysis of fibroblasts - (reply: 1)

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