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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
632. Protein Sample Preparation - Western Bloting (reply: 1)
633. No signal at all - (reply: 16)
634. Centrifuge after lysing - (reply: 5)
635. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
636. "Diffuesed" bands of higher molecular weight - (reply: 6)
637. Dimeric form of a protein? - I want my monomer! (reply: 2)
638. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
639. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
640. how to get rabbit antibody for Western blotting - (reply: 2)
641. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
642. Not sure of what % to use for gels - (reply: 6)
643. Protein Size - (reply: 3)
644. no separation of MW marker on gel - (reply: 1)
645. Transfer basic protein to PVDF - (reply: 1)
646. Expired pre-cast gels - (reply: 4)
647. LDS sample buffer in 95C - (reply: 2)
648. disappearing high molecular protein and marker,thanks - (reply: 1)
649. Use of cold milk??? - Western blot (reply: 2)
650. Antibody compatibility between different species - (reply: 4)
651. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 4)
652. Wrong bands on the blot - (reply: 4)
653. Protein drag in IEF-WB - (reply: 3)
654. SDS-PAGE gel staining problem - (reply: 1)
655. sample buffer pH - its effect?? (reply: 12)
656. SDS-Page and DMSO - (reply: 2)
657. Help with Western Blotting of Keratin Proteins - (reply: 2)
658. Problems detecting my band! - (reply: 2)
659. How many times can I reuse Ponceau S? - (reply: 3)
660. sds-page problems - polymerization of acrylamide (reply: 5)

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