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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. freezing antibody lyophilised - (reply: 2)
632. Nitrocellulose membrane storage after transfer - (reply: 6)
633. western without tween - (reply: 3)
634. Bubbles on the marker after transfer - (reply: 2)
635. Missing patches on immunoblot - please help! - (reply: 7)
636. stupid question - (reply: 8)
637. GAPDH merging lanes - (reply: 1)
638. Band at same position using different primary antibodies - (reply: 3)
639. Western for very small protein - (reply: 3)
640. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
641. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
642. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
643. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
644. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
645. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
646. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
647. What cause "downshift" in SDS-PAGE? - (reply: 5)
648. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
649. Blue native PAGE problems - (reply: 1)
650. running problems SDS-PAGE gel (zymography) - (reply: 4)
651. Western unresolved lanes - (reply: 2)
652. Doing WB from scratch for the first time. Need some info. - (reply: 2)
653. Western blot bovine collagen typ I problem - (reply: 3)
654. no band, extremely clean blot - (reply: 1)
655. Acrylamide stock - (reply: 2)
656. low signal in WB - (reply: 3)
657. standard internal marker for quantification - (reply: 3)
658. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
659. IgG - unspecific band (reply: 3)
660. red ponceau - (reply: 3)

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