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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. IgG - unspecific band (reply: 3)
632. red ponceau - (reply: 3)
633. Noisy fluorescent gels - (reply: 1)
634. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
635. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
636. resolving and stucking gel solutions - (reply: 2)
637. bradford absorbance - (reply: 1)
638. protein degradation - (reply: 3)
639. Dry or not PVDF membrane after transfer - (reply: 2)
640. I see bands with western blot only the third day! - (reply: 2)
641. Western Blots on Membrane Proteins - (reply: 9)
642. Help- voltage going sky high- don't know why - (reply: 1)
643. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
644. efficient stripping buffer - (reply: 2)
645. quantification of a protein in tissue - (reply: 3)
646. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
647. Help - proteins faster than the dye front - (reply: 8)
648. Longer incubation in primary antibody - (reply: 2)
649. Cell Lysis Buffer - (reply: 6)
650. Strange Western blot background - (reply: 2)
651. question about denaturing - (reply: 1)
652. Secondary antibody recovery - (reply: 4)
653. Help! I left my blot in primary at room temp overnight - (reply: 1)
654. Help! I left my blot in primary at room temp overnight - (reply: 1)
655. Help with Twist antibody? - (reply: 1)
656. Denaturing protein before blotting - (reply: 1)
657. Lysis buffer selection - (reply: 2)
658. not completed transfer proteins on NT membrane - western blot (reply: 2)
659. loading buffer and western blot help! - (reply: 5)
660. "Sandy WB" - (reply: 9)

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