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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. SDS-PAGE Second interface - (reply: 3)
632. biorad's 30% bis/acrylamide storage - (reply: 2)
633. protein concentration per well - (reply: 6)
634. dot blot - Problem (reply: 1)
635. Clear native PAGE - (reply: 1)
636. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
637. Weak western signal for help - (reply: 13)
638. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
639. Western not working anymore - (reply: 15)
640. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
641. BN-PAGE possible with Tris-glycine - (reply: 1)
642. Western few questions - (reply: 1)
643. thiourea in 2D page - What's it for? (reply: 1)
644. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
645. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
646. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
647. Antibody works in immunofluorescence, but not in WB - (reply: 4)
648. B-actin as western blot -control - (reply: 4)
649. horrific western blots , please help - (reply: 3)
650. Trouble with actin, HA and CCND1 :(( - (reply: 1)
651. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
652. Lower MW bands than expected. - (reply: 1)
653. Question about reducing agent (DTT) - (reply: 1)
654. Collagen II western blotting - (reply: 1)
655. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
656. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
657. NUCLEAR PROTEIN PREPARATION - (reply: 1)
658. Lost protein from blot? - (reply: 1)
659. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
660. No SDS in gels - (reply: 12)

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