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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
632. Western blot running and loading samples - (reply: 6)
633. problems with His tagged protein western blots - (reply: 3)
634. Springerlink--Zymography - (reply: 3)
635. Methanol vs Isopropanol in fixing solution - (reply: 1)
636. Protein aggregates SDS-PAGE - (reply: 5)
637. probably DNA is disturbing the quantification of my sample - (reply: 3)
638. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
639. Optimising antibody concentration - hints and tips (reply: 1)
640. Lanes all run together - (reply: 5)
641. Protein Markers - (reply: 1)
642. Gel Running Crooked?? Please help.. - (reply: 7)
643. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
644. Strength of actin signal - How dark should the band be? (reply: 5)
645. Immunoprecipitation - (reply: 2)
646. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
647. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
648. SEmi-dry transfer blotting - (reply: 1)
649. Whole cell lysis of fibroblasts - (reply: 1)
650. SDS-PAGE: Strange Running - (reply: 8)
651. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
652. Different TBST pH's for WB incubation, why? - (reply: 1)
653. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
654. stability of the stripping buffer - (reply: 2)
655. small proteins have run ahead of tracking dye - (reply: 1)
656. cytoplasmic and nuclear extraction - (reply: 2)
657. Equal Protein Quantity - Loading onto Well (reply: 6)
658. Help, odd pattern - pic included - (reply: 3)
659. Fermentas prestained protein marker issue! - (reply: 7)
660. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)

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