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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. efficient stripping buffer - (reply: 2)
632. quantification of a protein in tissue - (reply: 3)
633. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
634. Help - proteins faster than the dye front - (reply: 8)
635. Longer incubation in primary antibody - (reply: 2)
636. Cell Lysis Buffer - (reply: 6)
637. Strange Western blot background - (reply: 2)
638. question about denaturing - (reply: 1)
639. Secondary antibody recovery - (reply: 4)
640. Help! I left my blot in primary at room temp overnight - (reply: 1)
641. Help! I left my blot in primary at room temp overnight - (reply: 1)
642. Help with Twist antibody? - (reply: 1)
643. Denaturing protein before blotting - (reply: 1)
644. Lysis buffer selection - (reply: 2)
645. not completed transfer proteins on NT membrane - western blot (reply: 2)
646. loading buffer and western blot help! - (reply: 5)
647. "Sandy WB" - (reply: 9)
648. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
649. SDS PAGE- HELP - Bands not visible (reply: 4)
650. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
651. Strange SDS gel after Western Blotting - (reply: 1)
652. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
653. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
654. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
655. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
656. Western blot fail, time and time again - (reply: 21)
657. Western blot of urine samples - Problems verifying loading (reply: 1)
658. BSA test for native PAGE - (reply: 2)
659. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
660. transfer: amperes or volts? - (reply: 6)

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