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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
632. Antibody works in immunofluorescence, but not in WB - (reply: 4)
633. B-actin as western blot -control - (reply: 4)
634. horrific western blots , please help - (reply: 3)
635. Trouble with actin, HA and CCND1 :(( - (reply: 1)
636. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
637. Lower MW bands than expected. - (reply: 1)
638. Question about reducing agent (DTT) - (reply: 1)
639. Collagen II western blotting - (reply: 1)
640. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
641. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
642. NUCLEAR PROTEIN PREPARATION - (reply: 1)
643. Lost protein from blot? - (reply: 1)
644. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
645. No SDS in gels - (reply: 12)
646. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
647. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
648. Sample loading buffer turning yellow. - (reply: 3)
649. western bands look funny - (reply: 5)
650. IgG SDS-PAGE - (reply: 1)
651. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
652. wet or semi-dry transfer for low MW proteins - (reply: 4)
653. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
654. quantifying "ghost" bands - (reply: 1)
655. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
656. Western blot running and loading samples - (reply: 6)
657. problems with His tagged protein western blots - (reply: 3)
658. Springerlink--Zymography - (reply: 3)
659. Methanol vs Isopropanol in fixing solution - (reply: 1)
660. Protein aggregates SDS-PAGE - (reply: 5)

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