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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
631. standard internal marker for quantification - (reply: 3)
632. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
633. IgG - unspecific band (reply: 3)
634. red ponceau - (reply: 3)
635. Noisy fluorescent gels - (reply: 1)
636. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
637. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
638. resolving and stucking gel solutions - (reply: 2)
639. bradford absorbance - (reply: 1)
640. protein degradation - (reply: 3)
641. Dry or not PVDF membrane after transfer - (reply: 2)
642. I see bands with western blot only the third day! - (reply: 2)
643. Western Blots on Membrane Proteins - (reply: 9)
644. Help- voltage going sky high- don't know why - (reply: 1)
645. how to determine gel percentage? - cleaved caspase 3 (reply: 1)
646. efficient stripping buffer - (reply: 2)
647. quantification of a protein in tissue - (reply: 3)
648. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
649. Help - proteins faster than the dye front - (reply: 8)
650. Longer incubation in primary antibody - (reply: 2)
651. Cell Lysis Buffer - (reply: 6)
652. Strange Western blot background - (reply: 2)
653. question about denaturing - (reply: 1)
654. Secondary antibody recovery - (reply: 4)
655. Help! I left my blot in primary at room temp overnight - (reply: 1)
656. Help! I left my blot in primary at room temp overnight - (reply: 1)
657. Help with Twist antibody? - (reply: 1)
658. Denaturing protein before blotting - (reply: 1)
659. Lysis buffer selection - (reply: 2)
660. not completed transfer proteins on NT membrane - western blot (reply: 2)

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