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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Western blot and isoelectric point - (reply: 1)
332. Western Blot Developing Cassette - (reply: 1)
333. Pull down assay and Co- IP experiment - (reply: 6)
334. Trouble detecting Histaq - (reply: 4)
335. Western gels running super slow! - (reply: 3)
336. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
337. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
338. WB parametric or non-parametric - (reply: 6)
339. Trouble with the GFP signal - (reply: 1)
340. Intern Needing Help - (reply: 3)
341. SDS-Electrophoresis, Dye color change - (reply: 1)
342. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
343. Trouble with equal loading - (reply: 8)
344. how many times striping can b done of the blot - (reply: 9)
345. SDS-PAGE of negatively charged protein - (reply: 4)
346. Protection Lanes? - (reply: 4)
347. Tie-2 westerns high background black lanes - (reply: 13)
348. BSA concentration for blocking - (reply: 5)
349. Identically loaded western bands loose intensity from left to right - (reply: 3)
350. eIF4G western blot - (reply: 3)
351. smear bands in western blot - (reply: 12)
352. Huge background with HT22 cells phophoERK antibody - (reply: 3)
353. How to quantify the protein amount for serum samples ? - (reply: 1)
354. Huge background with HT22 cells phophoERK antibody - (reply: 1)
355. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
356. horizontal smear? - (reply: 2)
357. BSA versus milk in blocking and antibody incubation - (reply: 5)
358. Western blots are 'semi' quantitative? - (reply: 12)
359. bands fuse together - (reply: 1)
360. Phospho-protein detection - (reply: 2)

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