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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. How to store a membrane? - (reply: 1)
332. Sample does not sit in the well despite (glycerol checked) - (reply: 4)
333. Western blot sample loading question - (reply: 1)
334. help for the method of meat sample preperation for SDS PAGE 1D - (reply: 4)
335. stripping too much? - (reply: 1)
336. sample precipitation problem in western blotting - (reply: 2)
337. Problem: mouse antibodies with mouse tissues - (reply: 4)
338. which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
339. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
340. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
341. Reuse of SDS-PAGE electrode buffers - (reply: 2)
342. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
343. Western transfer tank heating up - (reply: 2)
344. Tricine PAG - (reply: 1)
345. Simple Western = a western blot revolution? - (reply: 2)
346. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
347. Best phospho-ERK antibody - (reply: 3)
348. what are the best transfer conditions? - (reply: 4)
349. Cell Envelope Proteins Smeared - (reply: 1)
350. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
351. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
352. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
353. restaining of coommassie destained gel - (reply: 1)
354. Secondary antibody overnight? - (reply: 5)
355. Problems with protein estimation and western blots - (reply: 1)
356. Loading controls - (reply: 1)
357. Quantity of protein to charge - (reply: 1)
358. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
359. Tris-Hcl becomes cloudy. - (reply: 3)
360. Can FLAG IP, but cannot detect with Western - (reply: 4)

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