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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Protein bands not migrating - (reply: 3)
332. Help with high background (have tried most trouble shooting) - (reply: 8)
333. Tumor protein precipitation at denaturing step? - (reply: 1)
334. How to prepare blood serum for western blot? - (reply: 3)
335. TNF alpha and IL 1b Western Blot - (reply: 1)
336. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
337. SDS-PAGE trouble with protein concentration - (reply: 2)
338. Acrylamid - (reply: 5)
339. Detecting doublet bands in Western for eGFP control - (reply: 1)
340. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
341. Protein Size 50 kDa higher than expected - (reply: 3)
342. Acid urea gel electrophoresis (AUGE) - (reply: 7)
343. Phospho-protein vs total protein Western blot - (reply: 5)
344. storing the loaded gel before running it - (reply: 7)
345. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
346. Western blot trouble shooting - (reply: 3)
347. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
348. two bands on WB-alternative use of start codons? - (reply: 2)
349. TCA Precipitation to remove albumin - (reply: 6)
350. adjusting pH of 4x stacking buffer - (reply: 3)
351. No WB bands for H2AX at all...! - (reply: 10)
352. any tips for doing Fluorescence Western Blot? - (reply: 2)
353. Unknown silver/grey residue left after transfer. - (reply: 2)
354. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
355. Changes to housekeeping loading controls after drug treatment - (reply: 2)
356. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
357. Multiple bands on blot with one antibody - (reply: 1)
358. sedimentation of cytosolic proteins - (reply: 2)
359. Determining pI of a protein - (reply: 3)
360. Non-Reduced Western Blot - (reply: 5)

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