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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Tris-Hcl becomes cloudy. - (reply: 3)
332. Can FLAG IP, but cannot detect with Western - (reply: 4)
333. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
334. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
335. Native PAGE, interaction of proteins, validity - (reply: 4)
336. Smeared transfer on nitrocellulose membrane - (reply: 2)
337. blocking and primary antibody in different buffers? - (reply: 1)
338. Same non specific band for different target proteins - (reply: 1)
339. Types of 96-well plates used for BCA protein quantification - (reply: 1)
340. Dot blot halo - (reply: 4)
341. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
342. Western blot and isoelectric point - (reply: 1)
343. Western Blot Developing Cassette - (reply: 1)
344. Pull down assay and Co- IP experiment - (reply: 6)
345. Trouble detecting Histaq - (reply: 4)
346. Western gels running super slow! - (reply: 3)
347. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
348. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
349. WB parametric or non-parametric - (reply: 6)
350. Trouble with the GFP signal - (reply: 1)
351. Intern Needing Help - (reply: 3)
352. SDS-Electrophoresis, Dye color change - (reply: 1)
353. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
354. Trouble with equal loading - (reply: 8)
355. how many times striping can b done of the blot - (reply: 9)
356. SDS-PAGE of negatively charged protein - (reply: 4)
357. Protection Lanes? - (reply: 4)
358. Tie-2 westerns high background black lanes - (reply: 13)
359. BSA concentration for blocking - (reply: 5)
360. Identically loaded western bands loose intensity from left to right - (reply: 3)

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