Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
332. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
333. Reuse of SDS-PAGE electrode buffers - (reply: 2)
334. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
335. Western transfer tank heating up - (reply: 2)
336. Tricine PAG - (reply: 1)
337. Simple Western = a western blot revolution? - (reply: 2)
338. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
339. Best phospho-ERK antibody - (reply: 3)
340. what are the best transfer conditions? - (reply: 4)
341. Cell Envelope Proteins Smeared - (reply: 1)
342. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
343. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
344. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
345. restaining of coommassie destained gel - (reply: 1)
346. Secondary antibody overnight? - (reply: 5)
347. Problems with protein estimation and western blots - (reply: 1)
348. Loading controls - (reply: 1)
349. Quantity of protein to charge - (reply: 1)
350. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
351. Tris-Hcl becomes cloudy. - (reply: 3)
352. Can FLAG IP, but cannot detect with Western - (reply: 4)
353. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
354. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
355. Native PAGE, interaction of proteins, validity - (reply: 4)
356. Smeared transfer on nitrocellulose membrane - (reply: 2)
357. blocking and primary antibody in different buffers? - (reply: 1)
358. Same non specific band for different target proteins - (reply: 1)
359. Types of 96-well plates used for BCA protein quantification - (reply: 1)
360. Dot blot halo - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34