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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. protein aggregation (?) in gelatin zymography - (reply: 1)
332. Use of wrong TBS concentration - (reply: 1)
333. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
334. SDS-PAGE staining for digestion assay - (reply: 1)
335. Can I improve my primary antibody? - (reply: 2)
336. got band in control positive but not in sample... - (reply: 2)
337. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
338. Problems with IPs - (reply: 2)
339. Band is at the wrong size, but same for all antibodies - (reply: 6)
340. Problems with western blot detection - (reply: 1)
341. Methods of concentrating total protein extracts - (reply: 2)
342. Using serum as the sample in Western blot - (reply: 3)
343. Puzzeling western blot results, missing band - (reply: 3)
344. Secondary AB blocking - (reply: 1)
345. western blood immunodetection Ab - (reply: 1)
346. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
347. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
348. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
349. What percentage should I use for a stacking gel? - (reply: 1)
350. DMSO - (reply: 1)
351. several questions on running SDS-PAGE - (reply: 1)
352. Voltage issues during transfer - (reply: 5)
353. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
354. What percentage gel should I make? - (reply: 1)
355. Western Blot problems-vertical streaking - (reply: 3)
356. Protein extraction from dead cells - (reply: 1)
357. Signal stuck on top of seperating gel - (reply: 1)
358. SDS-PAGE gel and running buffer - (reply: 3)
359. The same strong bands with different primary antibody - (reply: 1)
360. Trouble Western Blotting a 360kDa Protein - (reply: 5)

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