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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. what are the best transfer conditions? - (reply: 4)
332. Cell Envelope Proteins Smeared - (reply: 1)
333. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
334. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
335. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
336. restaining of coommassie destained gel - (reply: 1)
337. Secondary antibody overnight? - (reply: 5)
338. Problems with protein estimation and western blots - (reply: 1)
339. Loading controls - (reply: 1)
340. Quantity of protein to charge - (reply: 1)
341. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
342. Tris-Hcl becomes cloudy. - (reply: 3)
343. Can FLAG IP, but cannot detect with Western - (reply: 4)
344. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
345. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
346. Native PAGE, interaction of proteins, validity - (reply: 4)
347. Smeared transfer on nitrocellulose membrane - (reply: 2)
348. blocking and primary antibody in different buffers? - (reply: 1)
349. Same non specific band for different target proteins - (reply: 1)
350. Types of 96-well plates used for BCA protein quantification - (reply: 1)
351. Dot blot halo - (reply: 4)
352. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
353. Western blot and isoelectric point - (reply: 1)
354. Western Blot Developing Cassette - (reply: 1)
355. Pull down assay and Co- IP experiment - (reply: 6)
356. Trouble detecting Histaq - (reply: 4)
357. Western gels running super slow! - (reply: 3)
358. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
359. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
360. WB parametric or non-parametric - (reply: 6)

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