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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
332. Western transfer tank heating up - (reply: 2)
333. Tricine PAG - (reply: 1)
334. Simple Western = a western blot revolution? - (reply: 2)
335. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
336. Best phospho-ERK antibody - (reply: 3)
337. what are the best transfer conditions? - (reply: 4)
338. Cell Envelope Proteins Smeared - (reply: 1)
339. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
340. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
341. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
342. restaining of coommassie destained gel - (reply: 1)
343. Secondary antibody overnight? - (reply: 5)
344. Problems with protein estimation and western blots - (reply: 1)
345. Loading controls - (reply: 1)
346. Quantity of protein to charge - (reply: 1)
347. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
348. Tris-Hcl becomes cloudy. - (reply: 3)
349. Can FLAG IP, but cannot detect with Western - (reply: 4)
350. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
351. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
352. Native PAGE, interaction of proteins, validity - (reply: 4)
353. Smeared transfer on nitrocellulose membrane - (reply: 2)
354. blocking and primary antibody in different buffers? - (reply: 1)
355. Same non specific band for different target proteins - (reply: 1)
356. Types of 96-well plates used for BCA protein quantification - (reply: 1)
357. Dot blot halo - (reply: 4)
358. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
359. Western blot and isoelectric point - (reply: 1)
360. Western Blot Developing Cassette - (reply: 1)

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