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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
332. Changes to housekeeping loading controls after drug treatment - (reply: 2)
333. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
334. Multiple bands on blot with one antibody - (reply: 1)
335. sedimentation of cytosolic proteins - (reply: 2)
336. Determining pI of a protein - (reply: 3)
337. Non-Reduced Western Blot - (reply: 5)
338. Incomplete transfer - (reply: 5)
339. can coommassie stained gel be western blot - (reply: 5)
340. Wet transfer: western blot - (reply: 3)
341. Reprobing a PVDF membrane which has gone dry - (reply: 1)
342. Non-specific bands and fuzzy bands - (reply: 1)
343. protein showing up around 50 kda rather than 20 kda - (reply: 2)
344. Help for western blot wet transfer blurry bands - (reply: 2)
345. anti-BRCA1 antibody specificity issues - (reply: 2)
346. Concentrating a diluted protein and protein determination - (reply: 4)
347. Blurry bands ... - (reply: 12)
348. Dot blotting problems - (reply: 3)
349. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
350. Uneven lane widths during western blots - (reply: 10)
351. Using human serum as first antibody in Western/ELISA - (reply: 3)
352. Strip Western Blotting Troubleshooting - (reply: 2)
353. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
354. Phospho-ATF-2 coming up different sizes - (reply: 1)
355. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
356. A possible protocol for western blotting troubleshooting? - (reply: 1)
357. Vertical Vs horizontal electrophoresis - (reply: 3)
358. Reprobing with the same antibody - (reply: 3)
359. SDS-PAGE problem with staining yellow background - (reply: 3)
360. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)

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