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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Dot blot halo - (reply: 4)
332. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
333. Western blot and isoelectric point - (reply: 1)
334. Western Blot Developing Cassette - (reply: 1)
335. Pull down assay and Co- IP experiment - (reply: 6)
336. Trouble detecting Histaq - (reply: 4)
337. Western gels running super slow! - (reply: 3)
338. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
339. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
340. WB parametric or non-parametric - (reply: 6)
341. Trouble with the GFP signal - (reply: 1)
342. Intern Needing Help - (reply: 3)
343. SDS-Electrophoresis, Dye color change - (reply: 1)
344. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
345. Trouble with equal loading - (reply: 8)
346. how many times striping can b done of the blot - (reply: 9)
347. SDS-PAGE of negatively charged protein - (reply: 4)
348. Protection Lanes? - (reply: 4)
349. Tie-2 westerns high background black lanes - (reply: 13)
350. BSA concentration for blocking - (reply: 5)
351. Identically loaded western bands loose intensity from left to right - (reply: 3)
352. eIF4G western blot - (reply: 3)
353. smear bands in western blot - (reply: 12)
354. Huge background with HT22 cells phophoERK antibody - (reply: 3)
355. How to quantify the protein amount for serum samples ? - (reply: 1)
356. Huge background with HT22 cells phophoERK antibody - (reply: 1)
357. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
358. horizontal smear? - (reply: 2)
359. BSA versus milk in blocking and antibody incubation - (reply: 5)
360. Western blots are 'semi' quantitative? - (reply: 12)

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