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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
331. Incomplete transfer - (reply: 5)
332. can coommassie stained gel be western blot - (reply: 5)
333. Wet transfer: western blot - (reply: 3)
334. Reprobing a PVDF membrane which has gone dry - (reply: 1)
335. Non-specific bands and fuzzy bands - (reply: 1)
336. protein showing up around 50 kda rather than 20 kda - (reply: 2)
337. Help for western blot wet transfer blurry bands - (reply: 2)
338. anti-BRCA1 antibody specificity issues - (reply: 2)
339. Concentrating a diluted protein and protein determination - (reply: 4)
340. Blurry bands ... - (reply: 12)
341. Dot blotting problems - (reply: 3)
342. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
343. Uneven lane widths during western blots - (reply: 10)
344. Using human serum as first antibody in Western/ELISA - (reply: 3)
345. Strip Western Blotting Troubleshooting - (reply: 2)
346. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
347. Phospho-ATF-2 coming up different sizes - (reply: 1)
348. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
349. A possible protocol for western blotting troubleshooting? - (reply: 1)
350. Vertical Vs horizontal electrophoresis - (reply: 3)
351. Reprobing with the same antibody - (reply: 3)
352. SDS-PAGE problem with staining yellow background - (reply: 3)
353. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
354. Western blot narrow bands - (reply: 1)
355. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
356. Western Blot Antibody Epitope - (reply: 1)
357. Western blot band anaylisis - (reply: 3)
358. protein aggregation (?) in gelatin zymography - (reply: 1)
359. Use of wrong TBS concentration - (reply: 1)
360. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)

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