Finding suitable Acrylamide/Bis-acrylamide ratio - (Sep/17/2015 )
I will be doing protein expression work and will need to run SDS soon.
Right now I am working on a 27 kDa protein and soon my work will be on a 63kDa protein.
But I am confused to which ratio of acrylamide/Bis-acrylamide premixed solution (Biorad) to buy.
How do I know which ratio is suitable for my protein?
These sound like very typical protein sizes, so a normal 19:1 ratio would probably work well.
i've always found that 37.5:1 is pretty standard for protein page although thermo-fisher seems to recommend 19:1.
however, i think you are also asking what should be the total acrylamide concentration for the gel. assuming that you are only interested in the 2 sizes you give, you can probably use a 10 or 12% gel.