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Tissue Related Discussions
  1. PFA fixed, sucrose dehydration then cryosectioned. Is the tissue usable for immu - (reply: 1)
  2. Identification of cells and tissue - (reply: 2)
  3. Can I snap freeze my tissue (liquid nitrogen) in Lysis buffer for later RNA extr - (reply: 6)
  4. Fresh tissue preservation for mid to long term. - (reply: 3)
  5. Tissue homogenization using Potassium phosphate buffer - (reply: 1)
  6. Preservation of human tissue for bacterial mRNA isolation from it later - (reply: 2)
  7. Sealing hole around tubing to enter side window of tissue culture cabinet - (reply: 1)
  8. soft tissue homogenization - (reply: 1)
  9. Increasing RNA yield from very small tissues - (reply: 3)
  10. Testing primers on 'unknown' tissue - (reply: 1)
  11. Frozen tissue, could it be possible to make parafin or OCT blocks? - (reply: 4)
  12. Tissue Preservation - (reply: 1)
  13. tissue homogenization problem - (reply: 5)
  14. Alternatives to mouse antibody for mouse tissue - (reply: 1)
  15. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  16. Species/Tissue Specific Processing Protocols - (reply: 1)
  17. Protein isolation for tissue stored in RNAlater - (reply: 1)
  18. Do you go by 'total protein' or 'tissue weight'? - (reply: 1)
  19. Degradation of fixed tissue sections - (reply: 4)
  20. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
  21. Calculations for ELISA of tissue lysate - (reply: 2)
  22. copies per ul to copies per gram tissue - (reply: 2)
  23. Storage of Adipose Tissue - (reply: 4)
  24. rna isolation from formalin fixed peg tissue - (reply: 2)
  25. Protein extracted from RNAlater tissues is blue... Help! - (reply: 2)
  26. How to ship plant tissue for RNA extraction? - (reply: 2)
  27. tissue collection and transport medium - (reply: 3)
  28. How to calculate pg/ml/mg for tissue culture? - (reply: 4)
  29. What happens if we use expired tissue culture flasks (T-162 Flasks) for vero cel - (reply: 1)
  30. Deparaffinization, tissue incubation over night in 85 % ethanol - (reply: 1)
  31. miRNA sequencing from FFPE tissue - (reply: 3)
  32. Nuclear extracts from frozen adipose tissue - (reply: 1)
  33. protein extraction and peptide quantification from fat breast tissue - (reply: 2)
  34. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
  35. Carbon release from human tissue? - (reply: 3)
  36. Need Sterile Technique for Fruit Tissue Storage - (reply: 4)
  37. Viral RNA extraction/isolation methods from plant tissue - (reply: 4)
  38. Not wearing gloves during tissue culture - (reply: 5)
  39. Lipid extraction from parrafin embedded tissue ? - (reply: 1)
  40. blood contamination in tissue - (reply: 1)
  41. Short sequence tissue specific BLAST - (reply: 1)
  42. Tissue Homogenate - (reply: 1)
  43. Generation of single cell suspensions from neural tissue - (reply: 2)
  44. Vibratome (~ 150um thick slice) to identify GFP signal in the tissues - (reply: 2)
  45. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
  46. how to amplify a fungus which is in the host tissue - (reply: 3)
  47. Histone extraction from brain tissue - (reply: 1)
  48. suggestions for making use of tissue slides - (reply: 3)
  49. DNA Isolation from Mouse Tissue - (reply: 1)
  50. Difficulty in cutting tissue after collection of sample in RNAlater - (reply: 3)
  51. Protocol for flow cytometry (FACS) anlaysis of xenograft tumor tissue - (reply: 3)
  52. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
  53. insulin concentration for tissue culture - (reply: 3)
  54. Low 260/230 ratio depending on tissue. - (reply: 4)
  55. can we extract DNA from snap freeze tissue - (reply: 2)
  56. Protein extraction from mouse tissue? - (reply: 1)
  57. Grevillea Acanthifolia Tissue Culture Help - (reply: 1)
  58. 3D MAMMALIAN TISSUE culture - (reply: 1)
  59. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  60. Discrepancy between tissue and cell culture - (reply: 1)
  61. How to thoroughly solubilize proteins from tissue sample, for quantitation - (reply: 1)
  62. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
  63. Degraded DNA from Mouse Tissues - (reply: 5)
  64. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
  65. FFPE tissue and RNA isolation - (reply: 2)
  66. SDS-PAGE w/ PFA fixed tissue - (reply: 4)
  67. brain tissue homogenization with sample buffer?? - (reply: 2)
  68. Establishing a primary tissue culture from surgically excised tumor - (reply: 1)
  69. gDNA Isolation from Human Breast Tissue - (reply: 2)
  70. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
  71. lung tissue storage - (reply: 1)
  72. Can I go for ChP having frozen tissue - (reply: 4)
  73. Storage of tissue at -80 followed by a breif unthaw prior to extraction - (reply: 1)
  74. Isolation of both DNA and RNA from the same plant tissue - (reply: 8)
  75. Problem: mouse antibodies with mouse tissues - (reply: 4)
  76. latest research in plant tissue culture field - (reply: 1)
  77. Age of paraffin embedded tissue - (reply: 1)
  78. brain and colon tissue homogenate for WB - (reply: 3)
  79. isolation of an already frozen tissue sample - (reply: 1)
  80. dna extraction from plant tissue infected with fungus - (reply: 1)
  81. Need replacement spin columns for Qiagen's Blood and Tissue DNeasy Kit - (reply: 4)
  82. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  83. Potato tissue culture pH - (reply: 5)
  84. cytokine levels in brain tissue - (reply: 1)
  85. RNA extraction from paraffin embedded tissue - (reply: 2)
  86. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
  87. DNA extraction and paraffin embedded tissue - (reply: 5)
  88. What antifungal to use in Tissue Culture??? - (reply: 2)
  89. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  90. Simultaneous Double Staining in paraffin tissues - (reply: 2)
  91. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  92. ChIP from mouse tissues with antibody raised in mouse - (reply: 1)
  93. Separating brain, gland and muscle tissues pre-RNA extaction - (reply: 1)
  94. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  95. GFP non-fluorescent on tissue - (reply: 6)
  96. Fixing tissue for cryosectioning - (reply: 4)
  97. Tissue culture treatment of cover slip - Tissue culture treatment without using amine/ thiol containing buffers (reply: 5)
  98. Tissue disruption - for RNA isolation (reply: 2)
  99. Tissue culture hood vacuum (aspiration) tubing - What is the best way for storing it between uses? (reply: 1)
  100. Opening a sterile reagent out of the tissue culture hood - (reply: 4)
  101. brain tissue preparation for immunoblot - (reply: 1)
  102. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
  103. Tissues used to genotyping animals - What tissues do you take for genotyping? (reply: 3)
  104. Three questions about skeletal muscle tissue - (reply: 2)
  105. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
  106. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
  107. Should I fix cells digested from tissues? - (reply: 4)
  108. X-gal stained tissue for IFC? - Will this work? (reply: 2)
  109. Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
  110. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
  111. Brain tissue homogenization method - (reply: 2)
  112. ten broek tissue grinder - (reply: 1)
  113. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  114. garlic tissue culture final project - (reply: 2)
  115. And how to grind a tissue with liquid nitrogen? - (reply: 3)
  116. Protein extraction from perfusion fixed brain tissue - (reply: 5)
  117. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  118. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  119. DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (reply: 2)
  120. quantification of a protein in tissue - (reply: 3)
  121. Extraction protein from paraffin-embedded tissues - (reply: 1)
  122. DNA ext from paraffin-embedded tissue ... a dilemma ! - FFPE tissue refuse to lyse strange whitish clumps formed!! (reply: 1)
  123. How to homogenise tissue in buffer? - (reply: 2)
  124. IHC: frozen sections or paraffin-embedded tissues? - (reply: 3)
  125. storing fixed leaf tissue - (reply: 1)
  126. Harvesting Mouse Organs for Mouse Tissue Extracts - (reply: 2)
  127. Tissue culture contamination - can't get rid of it!!! (reply: 6)
  128. Histology on adipose tissue - (reply: 1)
  129. what type of media for gingiva fibroblast tissue culture? - (reply: 3)
  130. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
  131. Cryopreservation of tissue in 50mL centrifuge tube (Urgent) - (reply: 2)
  132. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
  133. how to expression mirna in a specific tissue ? - (reply: 1)
  134. Tissues embeded in paraffin wax - 2 yes/no question- can't understand or get idea online! help plz plz (reply: 1)
  135. acetone dehydration of tissue - (reply: 1)
  136. extraction of viral rna from plant tissue - (reply: 1)
  137. Preparing Frozen Tissue Sections - (reply: 3)
  138. protein extraction from tissue, help - (reply: 1)
  139. Protein extraction from tissue - (reply: 1)
  140. is it necessary to use RNAlater for tissue samples? - (reply: 1)
  141. Cell Suspension from Frozen Tissue? - (reply: 6)
  142. tissue disruption help... - beads that are more than beads (reply: 2)
  143. adding fungicidies to the media in plant tissue culture - (reply: 2)
  144. About tissue culture 96 well plates - (reply: 4)
  145. Homogenising tissue, what do you rinse with? - (reply: 4)
  146. Extracting precious tissue from too large of volumes of RNAlater - stored tissue in too large of volume of RNAlater, can't recover (reply: 5)
  147. Can you remove OCT from tissue? - (reply: 2)
  148. Fixing tissue - (reply: 7)
  149. Brain fixation for electron microscopy - Can PFA-fixed autopsy tissues be examined with electron microscopy? (reply: 1)
  150. tissue culture and its problem??? - (reply: 6)
  151. presentation about plant tissue culture - (reply: 1)
  152. RNA Extraction Mice Adipose Tissue - (reply: 3)
  153. Hydrogen Peroxide (H2O2) Generator - Decontaminating tissue culture lab with H2O2. (reply: 1)
  154. BSP PCR for help - no bands of tissue amplification (reply: 3)
  155. coating meat tissue to ELISA plates - (reply: 3)
  156. GAPDH Tissue Distribution - (reply: 1)
  157. Do I need to dilute my mouse tissue lysates for micro-BCA? - (reply: 1)
  158. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  159. Preparation of tree tissue for western blot - (reply: 1)
  160. Homogenation of Mouse Spinal Cord Tissue for RNA Extraction - Most effective way to do it? (reply: 3)
  161. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  162. dehydration and rehydration of tissues - (reply: 1)
  163. Vibrating microtome vs Tissue slicers - Which is better? (reply: 1)
  164. Low concentration alcohol as an antimicrobial - And the side effects on tissue (reply: 11)
  165. IHC for frozen section for cartilage tissue - (reply: 1)
  166. Qiagen DNAeasy blood&tissue DNA isolation kit - (reply: 1)
  167. western blot problem: cell culture VS mouse tissue - (reply: 4)
  168. Whole tissue starting product - not cultured cells - (reply: 2)
  169. DNA isolation from tissue !!SOS!! - (reply: 1)
  170. cell lines vs tissue expression - (reply: 1)
  171. Cryosection problem - How to prevent tissue from peeling off (reply: 2)
  172. How to separate fungus cells from human lung tissues - (reply: 7)
  173. Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
  174. Drosophila tissue homogenization - need help (reply: 3)
  175. handling retinal tissue for western blot analysis - (reply: 4)
  176. Co-IP and Western blot with snap-frozen brain tissues - (reply: 2)
  177. coating tissue culture lates with fibronectin - (reply: 1)
  178. Animal tissue ChIP - to freeze or not to freeze? - Can you freeze your tissue for ChIP? (reply: 3)
  179. Handling Tissue Samples for Dummies? - Acquisition, Storage, and Handling of Human Tissue Samples (reply: 1)
  180. Tissue & Cell Fixation solutions - (reply: 3)
  181. Tissue processing and embedding - (reply: 1)
  182. FACS Sample Prep from whole brain tissue (mouse) - (reply: 2)
  183. Limitations of Animal Tissue Cultures - (reply: 11)
  184. filter tips or regular tips for tissue culture? - (reply: 4)
  185. Treating glassware for tissue culture - (reply: 3)
  186. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
  187. Tissue sonication for RNA extraction - Help :) (reply: 1)
  188. How to wash ethanol away from tissue? - (reply: 3)
  189. Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 7)