Brain tissue homogenization method - (Feb/08/2011 )

Hello everybody! I want to isolate total RNA from specific mouse brain regions like striatum and hippocampus (5-50 mg). I would appreciate if you could recommend me a homogenizetion method for the dissected brain tissue. Thanks.

We use single use disposable teflon homogenization tips. After that, a good way to get rid of the sticky gDNA is to push it through a needle a couple of times.

BioMiha, thank you for the very quick reply! Can you give me more information about the single use teflon homogenization tips you use (brand, etc.). Is it motor-driven or by hand? Thank you!