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Do I need to dilute my mouse tissue lysates for micro-BCA? - (Sep/17/2009 )

Dear Lab Rats,

I have been preparing total protein lysates from spleen, liver and kidney (from mouse) and would like to know should I dilute the samples before adding to my micro-BCA assay (plate method)?

I am usually working with cell lines and dilute from 1:20-1:100 but I know my cell numbers in this situation.

I am hoping to be able to do the assay as a one off, instead of doing multiple titrations ... :)

Thanks for your suggestions,



I should mention that I use 3 ml RIPA buffer per gram of tissue :(