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Staining Related Discussions
  1. How to prep mouse urine samples for SDS-PAGE and Coomassie staining? - (reply: 2)
  2. unusual FITC background staining - (reply: 2)
  3. lower part of the gel was not stained - (reply: 7)
  4. Can microglia stain positive for phagocytosed material? - (reply: 2)
  5. Cytotoxicity of hoechst staining in overnight experiment - (reply: 1)
  6. Gram staining issues - (reply: 4)
  7. Use of fluorescent stain on IEF gels - (reply: 1)
  8. Annexin PI staining - (reply: 1)
  9. immunofluorescence staining protocol question - (reply: 1)
  10. protein staining with EtBr!! - (reply: 4)
  11. Syber green gel staining after electrophoresis - (reply: 4)
  12. Trypan Blue Staining - (reply: 1)
  13. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  14. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
  15. Use of Gram Stain kit with Reagents differentation - (reply: 2)
  16. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
  17. Staining SDS-PAGE gel with EtBr? - (reply: 6)
  18. polyA RNA staining for dot blot - (reply: 4)
  19. cell viability with PI staining - (reply: 2)
  20. silver stain destaining - (reply: 1)
  21. Spotted DAB staining! - (reply: 1)
  22. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
  23. Quantuitative staining of RNA and DNA? - (reply: 4)
  24. Karyotyping, Giemsa Staining - (reply: 4)
  25. How to differentiate Enterobacteriace on Gram Stain? - (reply: 3)
  26. Is possible that unknown culture came out negative for all the three staining te - (reply: 2)
  27. why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
  28. Quantification of the immunohistochemical staining slides - (reply: 4)
  29. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  30. In simple staining, what is the charge of the dye? - (reply: 1)
  31. PI staining cell cycle analysis - (reply: 1)
  32. Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
  33. 2 Photos - 2 Questions: Gram Stain - (reply: 4)
  34. H&E Staining help! - (reply: 1)
  35. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  36. Flow cytometry and immunofluorescent staining: help needed - (reply: 2)
  37. Low signal, weird background stains on blot - (reply: 5)
  38. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  39. Live cell sort DNA stain permeabilization - (reply: 1)
  40. Gram Staining: What can one see? - (reply: 3)
  41. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  42. Incomplete Blotting and staining of NC membrane - (reply: 7)
  43. Intracellular staining at 4C - HELP - (reply: 8)
  44. polyacrylamide gel with sybr green staining - (reply: 1)
  45. Intracellular staining of cytokines... crossreactivity? - (reply: 1)
  46. immunofluorescence - antibody choice for double staining - (reply: 2)
  47. Troubleshooting with silver staining - (reply: 3)
  48. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  49. Acid fast staining: need help - (reply: 5)
  50. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
  51. GFP staining with FACS - (reply: 1)
  52. co-IP stained with coomassie blue - (reply: 2)
  53. Losing cells during intracellular staining method! - (reply: 1)
  54. Smeared Bands after Ponceau Staining? - (reply: 3)
  55. coomassie blue stain - (reply: 1)
  56. How do you reduce crystal violet staining background on plates? - (reply: 1)
  57. Transwell migration assay (Boyden chambers) and DAPI staining - (reply: 1)
  58. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  59. Stain in electrophoresis is going from + to - - (reply: 3)
  60. Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot - (reply: 1)
  61. Looking for simple Papanicolaou (pap) staining protocol - (reply: 2)
  62. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
  63. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  64. Problem with Gamma H2AX foci staining - (reply: 5)
  65. Cell number issue - Nile Red staining - (reply: 3)
  66. Western blot and immunofluorescence staining - (reply: 9)
  67. Integrin B1 Staining in HT1080 cells - (reply: 1)
  68. Intracellular staining, isotype control not stable - (reply: 1)
  69. isoelctric gels hard to stain? - (reply: 2)
  70. Alizarin Red Staining on scaffolds... - (reply: 1)
  71. Mass-spec compatible silver stain - (reply: 2)
  72. viability/integrity stain of fungal cells - (reply: 5)
  73. NBT solution in X-Gal staining - (reply: 1)
  74. HELP-zebrafish antibody staining after day3 - (reply: 6)
  75. Please look at my gel picture, poor EthBr staining - (reply: 2)
  76. protein not stained by Coomassie Blue - (reply: 13)
  77. Flag Staining in U87 cells - (reply: 4)
  78. Cells detach during bodipy 493/503 staining - (reply: 1)
  79. Problem with sequential double IF staining with rabbit antibodies - (reply: 2)
  80. Distinguish murine and human cells by surface staining? - (reply: 1)
  81. Non-fluorescent live/dead stains for prokaryotes - (reply: 2)
  82. Live/Dead Staining of already Permeable Cells - (reply: 10)
  83. PVDF membrane silver staining protocol - (reply: 5)
  84. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
  85. Coomassie stain forzen and thawed gel? - (reply: 1)
  86. crystal violet staining - (reply: 6)
  87. non-specific staining using with Immunos - (reply: 3)
  88. CRYSTAL VIOLET STAINING - (reply: 3)
  89. can coommassie stained gel be western blot - (reply: 5)
  90. good humidified chambers for slide staining - (reply: 2)
  91. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  92. Phalloidin-Alexa Fluor 488 staining problem (huge background) - (reply: 1)
  93. Cell staining for fluorometric assay - (reply: 5)
  94. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  95. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  96. SDS-PAGE problem with staining yellow background - (reply: 3)
  97. What colour do sem*n stains glow under a black light? - (reply: 1)
  98. SDS-PAGE staining for digestion assay - (reply: 1)
  99. FACS staining of human macrophages derived from PBMCs - (reply: 3)
  100. quantify immunofluorescence staining - (reply: 1)
  101. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  102. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  103. DiI staining Fixation protocol - (reply: 1)
  104. Immunofluorescence Staining of Cells - (reply: 1)
  105. Immunofluorescence - unspecific staining - (reply: 9)
  106. Oil red O staining?? - (reply: 1)
  107. Nuclei staining background in collagen matrix - (reply: 1)
  108. Sphere staining protocol (agarose) - (reply: 3)
  109. DAB staining and GFP - (reply: 1)
  110. Actin staining - (reply: 1)
  111. Propidium iodide staining - (reply: 1)
  112. Trypan Blue Staining - (reply: 3)
  113. problem in making a proper smear for nile blue staining of an unknown yeast - (reply: 2)
  114. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  115. RNA extraction from blood stain - (reply: 1)
  116. Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati - (reply: 1)
  117. Searching for a live cell staining method - (reply: 1)
  118. ANNEXIN V staining for FLOW - (reply: 3)
  119. What positive control for intracellular staining? - (reply: 1)
  120. Simultaneous Double Staining in paraffin tissues - (reply: 2)
  121. Which stain is frequently used to identify Mycobacterium - (reply: 1)
  122. Forgetting iodine when gram staining - (reply: 1)
  123. intracellular cytokine staining - (reply: 1)
  124. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  125. X-gal staining and IHC against b-gal - (reply: 7)
  126. mass compatible silver staining - (reply: 1)
  127. Staining Cells in Suspension - (reply: 4)
  128. Bradford reagent for staining gels? - (reply: 1)
  129. Single stain control for 293 cell line - (reply: 4)
  130. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  131. Intracellular staining for IL-21 - (reply: 1)
  132. Annexin-V staining - (reply: 1)
  133. apoptosis assay - immunofluoresence staining (reply: 5)
  134. Pre-cutting before staining - How long before staining can samples be cut? (reply: 1)
  135. silver staining of ip sample - (reply: 1)
  136. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
  137. GFP cells intracellular staining - (reply: 1)
  138. gelatin staining with coomassie: how do I make it work? - (reply: 3)
  139. X-gal stained tissue for IFC? - Will this work? (reply: 2)
  140. ICC: exotic problem with staining, vGlut1 ans synapsin-1 - (reply: 2)
  141. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  142. Detection of Apoptosis by Annexin V PI staining - (reply: 1)
  143. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  144. Histochemical stain specific for transthyretin (prealbumin)??! - Anyone aware if there s such a thing? (reply: 2)
  145. Fixing cells before staining of surface markers - (reply: 1)
  146. TLC Visualizing Stains - (reply: 1)
  147. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  148. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  149. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  150. Negative control is positive for IF staining - (reply: 3)
  151. intracelluar staining without killing cells - (reply: 3)
  152. Hoechst Stain does it stain dead cells - (reply: 3)
  153. Diastase or Amylase Digestion - Negative for PAS stain (reply: 4)
  154. detection of mycoplasma with hoechst staining - (reply: 1)
  155. short question - FDG staining (reply: 2)
  156. Flow cytometry-frozen PBMCs - Surface and intracellular staining (reply: 5)
  157. Basic questions about PI staining in Flowcyto - (reply: 7)
  158. T helper differentiation and staining - Trouble with intracellular staining (reply: 6)
  159. Help Stained after blocking and won't unstain!!! - (reply: 1)
  160. immunochemical staining problem - i'm struggling to resolve this problem (reply: 1)
  161. Help-IHC nuclear staining - (reply: 2)
  162. imunofluerecence staining with Alexa 568 dye - interesting (reply: 1)
  163. No protein spots in 2D gel - Silver staining! - (reply: 17)
  164. RNA extraction from stained cells - (reply: 2)
  165. Ponceau Red Staining Problem - (reply: 2)
  166. How to stain for oxidative stress - (reply: 1)
  167. Roles of staining soluton components!!! - (reply: 6)
  168. Toluidine blue staining for cell culture - (reply: 1)
  169. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  170. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  171. Annexin and propidium staining - (reply: 1)
  172. GUS histochemistry assay - No staining present (reply: 1)
  173. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  174. how to stain suspension culture? - (reply: 1)
  175. silver stain of immunoprecipitate - (reply: 2)
  176. NK cell staining - (reply: 4)
  177. Disposal of coomassie stain - (reply: 3)
  178. flagella stain - (reply: 1)
  179. Silver stained 2-d gel BLOB!!! - (reply: 1)
  180. microglia staining - protocol for microglia staining (reply: 1)
  181. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  182. what is the measurement of Oil red O staining - (reply: 3)
  183. Surface Staining and Methanol Fixation - (reply: 1)
  184. Live stain with propidium iodide - Incubation time & temperature? (reply: 3)
  185. immunofluorescence staining of sperm cells - (reply: 2)
  186. pre stained marker issues - (reply: 2)
  187. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  188. Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
  189. IHC staining of secreted proteins - (reply: 3)
  190. strange band in coomassie blue staining - (reply: 3)
  191. FBS Annexin staining - (reply: 1)
  192. siRNA staining? - (reply: 1)
  193. SDS-PAGE gel staining problem - (reply: 1)
  194. High background in phalloidin staining - (reply: 2)
  195. histological staining for plant cell wall components - (reply: 1)
  196. Antibody staining - (reply: 6)
  197. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
  198. Pre stained markers - (reply: 4)
  199. Staining spores - (reply: 1)
  200. DAPI/Hoechst vital staining - (reply: 3)
  201. Only borders stained in Silver!!! - (reply: 7)
  202. co staining problem: same primary species - (reply: 7)
  203. viability test: fixation AFTER propidium iodide staining? - (reply: 1)
  204. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  205. Staining of GLycoproteins in IEF - (reply: 4)
  206. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  207. collagen staining in cell culture - (reply: 5)
  208. CFSE staining of mycobacteria - (reply: 1)
  209. RNA elution after stains all staining - (reply: 3)
  210. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  211. Help needed with IF staining. - (reply: 2)
  212. vacuolation in MDCK cells - Vacuolation after staining (reply: 4)
  213. PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
  214. Staining of Intracellular Cytokines - (reply: 6)
  215. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  216. Th17 Differentiation and Staining - (reply: 18)
  217. Annexin V staining in combination with other antibodies - (reply: 4)
  218. DAB Peroxidase staining - (reply: 3)
  219. Fixation after or before staining? - (reply: 3)
  220. Preabsorption of Ab with Ag still leads to staining in ICC - (reply: 7)
  221. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  222. cell cycle analysis PI staining shift - (reply: 1)
  223. Ponceau Staining Question - (reply: 1)
  224. Intracellular stain of nuclear receptor - (reply: 6)
  225. sox-2 staining - (reply: 2)
  226. CFSE staining protocol - (reply: 14)
  227. What stains red in trypan blue - (reply: 2)
  228. PKH26 staining mesenchymal stem cells - toxicity (reply: 1)
  229. Beta Catenin immunofluorescent staining - (reply: 1)
  230. smeared silver stainning on acidic native page - how can I sovle smeared silver staining? (reply: 3)
  231. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
  232. YFP staining - (reply: 2)
  233. Problem with double staining for FACS - (reply: 1)
  234. Ponceau staining after primary and secondary? - (reply: 5)
  235. staining cells - (reply: 3)
  236. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  237. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  238. Purpose of methanol in the Blue Silver stain - (reply: 3)
  239. Bacterial Staining and Counting - (reply: 1)
  240. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  241. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  242. types of gel stains - (reply: 7)
  243. Etbr staining methods - (reply: 6)
  244. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  245. surface staining of THP-1 cell - (reply: 2)
  246. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  247. ER stained with Ab looks bad... - (reply: 3)
  248. HUVEC surface antigen staining - (reply: 1)
  249. Phosphoproteins staining - (reply: 2)
  250. apoptotic bodies- DAPI staining - (reply: 6)
  251. Integrin 1 staining - (reply: 2)
  252. Staining for neural crest derived cells?? - (reply: 2)
  253. problem to stain just one cell line in coculture - (reply: 4)
  254. ATPase staining for different Myosins - Anyone got a good protocol? (reply: 3)
  255. Is immunoblotting possible after the gel has been silver stained - (reply: 2)