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Staining Related Discussions
  1. Gomori trichrome stain - (reply: 1)
  2. TRAP staining decalcified bone paraffin embedded, osteoclasts disappear - (reply: 2)
  3. Intestine tissue falling of slides during H and E staining - (reply: 1)
  4. How to prep mouse urine samples for SDS-PAGE and Coomassie staining? - (reply: 2)
  5. unusual FITC background staining - (reply: 2)
  6. lower part of the gel was not stained - (reply: 7)
  7. Can microglia stain positive for phagocytosed material? - (reply: 2)
  8. Cytotoxicity of hoechst staining in overnight experiment - (reply: 1)
  9. Gram staining issues - (reply: 4)
  10. Use of fluorescent stain on IEF gels - (reply: 1)
  11. Annexin PI staining - (reply: 1)
  12. immunofluorescence staining protocol question - (reply: 1)
  13. protein staining with EtBr!! - (reply: 4)
  14. Syber green gel staining after electrophoresis - (reply: 4)
  15. Trypan Blue Staining - (reply: 1)
  16. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  17. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
  18. Use of Gram Stain kit with Reagents differentation - (reply: 2)
  19. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
  20. Staining SDS-PAGE gel with EtBr? - (reply: 6)
  21. polyA RNA staining for dot blot - (reply: 4)
  22. cell viability with PI staining - (reply: 2)
  23. silver stain destaining - (reply: 1)
  24. Spotted DAB staining! - (reply: 1)
  25. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
  26. Quantuitative staining of RNA and DNA? - (reply: 4)
  27. Karyotyping, Giemsa Staining - (reply: 4)
  28. How to differentiate Enterobacteriace on Gram Stain? - (reply: 3)
  29. Is possible that unknown culture came out negative for all the three staining te - (reply: 2)
  30. why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
  31. Quantification of the immunohistochemical staining slides - (reply: 4)
  32. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  33. In simple staining, what is the charge of the dye? - (reply: 1)
  34. PI staining cell cycle analysis - (reply: 1)
  35. Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
  36. 2 Photos - 2 Questions: Gram Stain - (reply: 4)
  37. H&E Staining help! - (reply: 1)
  38. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  39. Flow cytometry and immunofluorescent staining: help needed - (reply: 2)
  40. Low signal, weird background stains on blot - (reply: 5)
  41. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  42. Live cell sort DNA stain permeabilization - (reply: 1)
  43. Gram Staining: What can one see? - (reply: 3)
  44. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  45. Incomplete Blotting and staining of NC membrane - (reply: 7)
  46. Intracellular staining at 4C - HELP - (reply: 8)
  47. polyacrylamide gel with sybr green staining - (reply: 1)
  48. Intracellular staining of cytokines... crossreactivity? - (reply: 1)
  49. immunofluorescence - antibody choice for double staining - (reply: 2)
  50. Troubleshooting with silver staining - (reply: 3)
  51. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  52. Acid fast staining: need help - (reply: 5)
  53. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
  54. GFP staining with FACS - (reply: 1)
  55. co-IP stained with coomassie blue - (reply: 2)
  56. Losing cells during intracellular staining method! - (reply: 1)
  57. Smeared Bands after Ponceau Staining? - (reply: 3)
  58. coomassie blue stain - (reply: 1)
  59. How do you reduce crystal violet staining background on plates? - (reply: 1)
  60. Transwell migration assay (Boyden chambers) and DAPI staining - (reply: 1)
  61. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  62. Stain in electrophoresis is going from + to - - (reply: 3)
  63. Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot - (reply: 1)
  64. Looking for simple Papanicolaou (pap) staining protocol - (reply: 2)
  65. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
  66. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  67. Problem with Gamma H2AX foci staining - (reply: 5)
  68. Cell number issue - Nile Red staining - (reply: 3)
  69. Western blot and immunofluorescence staining - (reply: 9)
  70. Integrin B1 Staining in HT1080 cells - (reply: 1)
  71. Intracellular staining, isotype control not stable - (reply: 1)
  72. isoelctric gels hard to stain? - (reply: 2)
  73. Alizarin Red Staining on scaffolds... - (reply: 1)
  74. Mass-spec compatible silver stain - (reply: 2)
  75. viability/integrity stain of fungal cells - (reply: 5)
  76. NBT solution in X-Gal staining - (reply: 1)
  77. HELP-zebrafish antibody staining after day3 - (reply: 6)
  78. Please look at my gel picture, poor EthBr staining - (reply: 2)
  79. protein not stained by Coomassie Blue - (reply: 13)
  80. Flag Staining in U87 cells - (reply: 4)
  81. Cells detach during bodipy 493/503 staining - (reply: 1)
  82. Problem with sequential double IF staining with rabbit antibodies - (reply: 2)
  83. Distinguish murine and human cells by surface staining? - (reply: 1)
  84. Non-fluorescent live/dead stains for prokaryotes - (reply: 2)
  85. Live/Dead Staining of already Permeable Cells - (reply: 10)
  86. PVDF membrane silver staining protocol - (reply: 5)
  87. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
  88. Coomassie stain forzen and thawed gel? - (reply: 1)
  89. crystal violet staining - (reply: 6)
  90. non-specific staining using with Immunos - (reply: 3)
  91. CRYSTAL VIOLET STAINING - (reply: 3)
  92. can coommassie stained gel be western blot - (reply: 5)
  93. good humidified chambers for slide staining - (reply: 2)
  94. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  95. Phalloidin-Alexa Fluor 488 staining problem (huge background) - (reply: 1)
  96. Cell staining for fluorometric assay - (reply: 5)
  97. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  98. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  99. SDS-PAGE problem with staining yellow background - (reply: 3)
  100. What colour do sem*n stains glow under a black light? - (reply: 1)
  101. SDS-PAGE staining for digestion assay - (reply: 1)
  102. FACS staining of human macrophages derived from PBMCs - (reply: 3)
  103. quantify immunofluorescence staining - (reply: 1)
  104. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  105. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  106. DiI staining Fixation protocol - (reply: 1)
  107. Immunofluorescence Staining of Cells - (reply: 1)
  108. Immunofluorescence - unspecific staining - (reply: 9)
  109. Oil red O staining?? - (reply: 1)
  110. Nuclei staining background in collagen matrix - (reply: 1)
  111. Sphere staining protocol (agarose) - (reply: 3)
  112. DAB staining and GFP - (reply: 1)
  113. Actin staining - (reply: 1)
  114. Propidium iodide staining - (reply: 1)
  115. Trypan Blue Staining - (reply: 3)
  116. problem in making a proper smear for nile blue staining of an unknown yeast - (reply: 2)
  117. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  118. RNA extraction from blood stain - (reply: 1)
  119. Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati - (reply: 1)
  120. Searching for a live cell staining method - (reply: 1)
  121. ANNEXIN V staining for FLOW - (reply: 3)
  122. What positive control for intracellular staining? - (reply: 1)
  123. Simultaneous Double Staining in paraffin tissues - (reply: 2)
  124. Which stain is frequently used to identify Mycobacterium - (reply: 1)
  125. Forgetting iodine when gram staining - (reply: 1)
  126. intracellular cytokine staining - (reply: 1)
  127. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  128. X-gal staining and IHC against b-gal - (reply: 7)
  129. mass compatible silver staining - (reply: 1)
  130. Staining Cells in Suspension - (reply: 4)
  131. Bradford reagent for staining gels? - (reply: 1)
  132. Single stain control for 293 cell line - (reply: 4)
  133. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  134. Intracellular staining for IL-21 - (reply: 1)
  135. Annexin-V staining - (reply: 1)
  136. apoptosis assay - immunofluoresence staining (reply: 5)
  137. Pre-cutting before staining - How long before staining can samples be cut? (reply: 1)
  138. silver staining of ip sample - (reply: 1)
  139. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
  140. GFP cells intracellular staining - (reply: 1)
  141. gelatin staining with coomassie: how do I make it work? - (reply: 3)
  142. X-gal stained tissue for IFC? - Will this work? (reply: 2)
  143. ICC: exotic problem with staining, vGlut1 ans synapsin-1 - (reply: 2)
  144. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  145. Detection of Apoptosis by Annexin V PI staining - (reply: 1)
  146. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  147. Histochemical stain specific for transthyretin (prealbumin)??! - Anyone aware if there s such a thing? (reply: 2)
  148. Fixing cells before staining of surface markers - (reply: 1)
  149. TLC Visualizing Stains - (reply: 1)
  150. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  151. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  152. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  153. Negative control is positive for IF staining - (reply: 3)
  154. intracelluar staining without killing cells - (reply: 3)
  155. Hoechst Stain does it stain dead cells - (reply: 3)
  156. Diastase or Amylase Digestion - Negative for PAS stain (reply: 4)
  157. detection of mycoplasma with hoechst staining - (reply: 1)
  158. short question - FDG staining (reply: 2)
  159. Flow cytometry-frozen PBMCs - Surface and intracellular staining (reply: 5)
  160. Basic questions about PI staining in Flowcyto - (reply: 7)
  161. T helper differentiation and staining - Trouble with intracellular staining (reply: 6)
  162. Help Stained after blocking and won't unstain!!! - (reply: 1)
  163. immunochemical staining problem - i'm struggling to resolve this problem (reply: 1)
  164. Help-IHC nuclear staining - (reply: 2)
  165. imunofluerecence staining with Alexa 568 dye - interesting (reply: 1)
  166. No protein spots in 2D gel - Silver staining! - (reply: 17)
  167. RNA extraction from stained cells - (reply: 2)
  168. Ponceau Red Staining Problem - (reply: 2)
  169. How to stain for oxidative stress - (reply: 1)
  170. Roles of staining soluton components!!! - (reply: 6)
  171. Toluidine blue staining for cell culture - (reply: 1)
  172. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  173. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  174. Annexin and propidium staining - (reply: 1)
  175. GUS histochemistry assay - No staining present (reply: 1)
  176. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  177. how to stain suspension culture? - (reply: 1)
  178. silver stain of immunoprecipitate - (reply: 2)
  179. NK cell staining - (reply: 4)
  180. Disposal of coomassie stain - (reply: 3)
  181. flagella stain - (reply: 1)
  182. Silver stained 2-d gel BLOB!!! - (reply: 1)
  183. microglia staining - protocol for microglia staining (reply: 1)
  184. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  185. what is the measurement of Oil red O staining - (reply: 3)
  186. Surface Staining and Methanol Fixation - (reply: 1)
  187. Live stain with propidium iodide - Incubation time & temperature? (reply: 3)
  188. immunofluorescence staining of sperm cells - (reply: 2)
  189. pre stained marker issues - (reply: 2)
  190. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  191. Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
  192. IHC staining of secreted proteins - (reply: 3)
  193. strange band in coomassie blue staining - (reply: 3)
  194. FBS Annexin staining - (reply: 1)
  195. siRNA staining? - (reply: 1)
  196. SDS-PAGE gel staining problem - (reply: 1)
  197. High background in phalloidin staining - (reply: 2)
  198. histological staining for plant cell wall components - (reply: 1)
  199. Antibody staining - (reply: 6)
  200. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
  201. Pre stained markers - (reply: 4)
  202. Staining spores - (reply: 1)
  203. DAPI/Hoechst vital staining - (reply: 3)
  204. Only borders stained in Silver!!! - (reply: 7)
  205. co staining problem: same primary species - (reply: 7)
  206. viability test: fixation AFTER propidium iodide staining? - (reply: 1)
  207. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  208. Staining of GLycoproteins in IEF - (reply: 4)
  209. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  210. collagen staining in cell culture - (reply: 5)
  211. CFSE staining of mycobacteria - (reply: 1)
  212. RNA elution after stains all staining - (reply: 3)
  213. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  214. Help needed with IF staining. - (reply: 2)
  215. vacuolation in MDCK cells - Vacuolation after staining (reply: 4)
  216. PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
  217. Staining of Intracellular Cytokines - (reply: 6)
  218. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  219. Th17 Differentiation and Staining - (reply: 18)
  220. Annexin V staining in combination with other antibodies - (reply: 4)
  221. DAB Peroxidase staining - (reply: 3)
  222. Fixation after or before staining? - (reply: 3)
  223. Preabsorption of Ab with Ag still leads to staining in ICC - (reply: 7)
  224. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  225. cell cycle analysis PI staining shift - (reply: 1)
  226. Ponceau Staining Question - (reply: 1)
  227. Intracellular stain of nuclear receptor - (reply: 6)
  228. sox-2 staining - (reply: 2)
  229. CFSE staining protocol - (reply: 14)
  230. What stains red in trypan blue - (reply: 2)
  231. PKH26 staining mesenchymal stem cells - toxicity (reply: 1)
  232. Beta Catenin immunofluorescent staining - (reply: 1)
  233. smeared silver stainning on acidic native page - how can I sovle smeared silver staining? (reply: 3)
  234. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
  235. YFP staining - (reply: 2)
  236. Problem with double staining for FACS - (reply: 1)
  237. Ponceau staining after primary and secondary? - (reply: 5)
  238. staining cells - (reply: 3)
  239. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  240. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  241. Purpose of methanol in the Blue Silver stain - (reply: 3)
  242. Bacterial Staining and Counting - (reply: 1)
  243. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  244. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  245. types of gel stains - (reply: 7)
  246. Etbr staining methods - (reply: 6)
  247. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  248. surface staining of THP-1 cell - (reply: 2)
  249. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  250. ER stained with Ab looks bad... - (reply: 3)
  251. HUVEC surface antigen staining - (reply: 1)
  252. Phosphoproteins staining - (reply: 2)
  253. apoptotic bodies- DAPI staining - (reply: 6)
  254. Integrin 1 staining - (reply: 2)
  255. Staining for neural crest derived cells?? - (reply: 2)
  256. problem to stain just one cell line in coculture - (reply: 4)
  257. ATPase staining for different Myosins - Anyone got a good protocol? (reply: 3)
  258. Is immunoblotting possible after the gel has been silver stained - (reply: 2)