Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Staining Related Discussions
  1. Is possible that unknown culture came out negative for all the three staining te - (reply: 2)
  2. why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
  3. Quantification of the immunohistochemical staining slides - (reply: 4)
  4. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  5. In simple staining, what is the charge of the dye? - (reply: 1)
  6. PI staining cell cycle analysis - (reply: 1)
  7. Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
  8. 2 Photos - 2 Questions: Gram Stain - (reply: 4)
  9. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  10. Flow cytometry and immunofluorescent staining: help needed - (reply: 2)
  11. Low signal, weird background stains on blot - (reply: 5)
  12. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  13. Live cell sort DNA stain permeabilization - (reply: 1)
  14. Gram Staining: What can one see? - (reply: 3)
  15. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  16. Incomplete Blotting and staining of NC membrane - (reply: 7)
  17. Intracellular staining at 4C - HELP - (reply: 8)
  18. polyacrylamide gel with sybr green staining - (reply: 1)
  19. Intracellular staining of cytokines... crossreactivity? - (reply: 1)
  20. immunofluorescence - antibody choice for double staining - (reply: 2)
  21. Troubleshooting with silver staining - (reply: 3)
  22. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  23. Acid fast staining: need help - (reply: 5)
  24. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
  25. GFP staining with FACS - (reply: 1)
  26. co-IP stained with coomassie blue - (reply: 2)
  27. Losing cells during intracellular staining method! - (reply: 1)
  28. Smeared Bands after Ponceau Staining? - (reply: 3)
  29. coomassie blue stain - (reply: 1)
  30. How do you reduce crystal violet staining background on plates? - (reply: 1)
  31. Transwell migration assay (Boyden chambers) and DAPI staining - (reply: 1)
  32. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  33. Stain in electrophoresis is going from + to - - (reply: 3)
  34. Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot - (reply: 1)
  35. Looking for simple Papanicolaou (pap) staining protocol - (reply: 2)
  36. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
  37. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  38. Problem with Gamma H2AX foci staining - (reply: 5)
  39. Cell number issue - Nile Red staining - (reply: 3)
  40. Western blot and immunofluorescence staining - (reply: 9)
  41. Integrin B1 Staining in HT1080 cells - (reply: 1)
  42. Intracellular staining, isotype control not stable - (reply: 1)
  43. isoelctric gels hard to stain? - (reply: 2)
  44. Alizarin Red Staining on scaffolds... - (reply: 1)
  45. Mass-spec compatible silver stain - (reply: 2)
  46. viability/integrity stain of fungal cells - (reply: 5)
  47. NBT solution in X-Gal staining - (reply: 1)
  48. HELP-zebrafish antibody staining after day3 - (reply: 6)
  49. Please look at my gel picture, poor EthBr staining - (reply: 2)
  50. protein not stained by Coomassie Blue - (reply: 13)
  51. Flag Staining in U87 cells - (reply: 4)
  52. Cells detach during bodipy 493/503 staining - (reply: 1)
  53. Problem with sequential double IF staining with rabbit antibodies - (reply: 2)
  54. Distinguish murine and human cells by surface staining? - (reply: 1)
  55. Non-fluorescent live/dead stains for prokaryotes - (reply: 2)
  56. Live/Dead Staining of already Permeable Cells - (reply: 10)
  57. PVDF membrane silver staining protocol - (reply: 5)
  58. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
  59. Coomassie stain forzen and thawed gel? - (reply: 1)
  60. crystal violet staining - (reply: 5)
  61. non-specific staining using with Immunos - (reply: 3)
  62. CRYSTAL VIOLET STAINING - (reply: 3)
  63. can coommassie stained gel be western blot - (reply: 5)
  64. good humidified chambers for slide staining - (reply: 2)
  65. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  66. Phalloidin-Alexa Fluor 488 staining problem (huge background) - (reply: 1)
  67. Cell staining for fluorometric assay - (reply: 5)
  68. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  69. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  70. SDS-PAGE problem with staining yellow background - (reply: 3)
  71. What colour do sem*n stains glow under a black light? - (reply: 1)
  72. SDS-PAGE staining for digestion assay - (reply: 1)
  73. FACS staining of human macrophages derived from PBMCs - (reply: 3)
  74. quantify immunofluorescence staining - (reply: 1)
  75. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  76. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  77. DiI staining Fixation protocol - (reply: 1)
  78. Immunofluorescence Staining of Cells - (reply: 1)
  79. Immunofluorescence - unspecific staining - (reply: 9)
  80. Oil red O staining?? - (reply: 1)
  81. Nuclei staining background in collagen matrix - (reply: 1)
  82. Sphere staining protocol (agarose) - (reply: 3)
  83. DAB staining and GFP - (reply: 1)
  84. Actin staining - (reply: 1)
  85. Propidium iodide staining - (reply: 1)
  86. Trypan Blue Staining - (reply: 3)
  87. problem in making a proper smear for nile blue staining of an unknown yeast - (reply: 2)
  88. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  89. RNA extraction from blood stain - (reply: 1)
  90. Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati - (reply: 1)
  91. Searching for a live cell staining method - (reply: 1)
  92. ANNEXIN V staining for FLOW - (reply: 3)
  93. What positive control for intracellular staining? - (reply: 1)
  94. Simultaneous Double Staining in paraffin tissues - (reply: 2)
  95. Which stain is frequently used to identify Mycobacterium - (reply: 1)
  96. Forgetting iodine when gram staining - (reply: 1)
  97. intracellular cytokine staining - (reply: 1)
  98. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  99. X-gal staining and IHC against b-gal - (reply: 7)
  100. mass compatible silver staining - (reply: 1)
  101. Staining Cells in Suspension - (reply: 4)
  102. Bradford reagent for staining gels? - (reply: 1)
  103. Single stain control for 293 cell line - (reply: 4)
  104. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  105. Intracellular staining for IL-21 - (reply: 1)
  106. Annexin-V staining - (reply: 1)
  107. apoptosis assay - immunofluoresence staining (reply: 5)
  108. Pre-cutting before staining - How long before staining can samples be cut? (reply: 1)
  109. silver staining of ip sample - (reply: 1)
  110. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
  111. GFP cells intracellular staining - (reply: 1)
  112. gelatin staining with coomassie: how do I make it work? - (reply: 3)
  113. X-gal stained tissue for IFC? - Will this work? (reply: 2)
  114. ICC: exotic problem with staining, vGlut1 ans synapsin-1 - (reply: 2)
  115. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  116. Detection of Apoptosis by Annexin V PI staining - (reply: 1)
  117. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  118. Histochemical stain specific for transthyretin (prealbumin)??! - Anyone aware if there s such a thing? (reply: 2)
  119. Fixing cells before staining of surface markers - (reply: 1)
  120. TLC Visualizing Stains - (reply: 1)
  121. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  122. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  123. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  124. Negative control is positive for IF staining - (reply: 3)
  125. intracelluar staining without killing cells - (reply: 3)
  126. Hoechst Stain does it stain dead cells - (reply: 3)
  127. Diastase or Amylase Digestion - Negative for PAS stain (reply: 4)
  128. detection of mycoplasma with hoechst staining - (reply: 1)
  129. short question - FDG staining (reply: 2)
  130. Flow cytometry-frozen PBMCs - Surface and intracellular staining (reply: 5)
  131. Basic questions about PI staining in Flowcyto - (reply: 7)
  132. T helper differentiation and staining - Trouble with intracellular staining (reply: 6)
  133. Help Stained after blocking and won't unstain!!! - (reply: 1)
  134. immunochemical staining problem - i'm struggling to resolve this problem (reply: 1)
  135. Help-IHC nuclear staining - (reply: 2)
  136. imunofluerecence staining with Alexa 568 dye - interesting (reply: 1)
  137. No protein spots in 2D gel - Silver staining! - (reply: 17)
  138. RNA extraction from stained cells - (reply: 2)
  139. Ponceau Red Staining Problem - (reply: 2)
  140. How to stain for oxidative stress - (reply: 1)
  141. Roles of staining soluton components!!! - (reply: 6)
  142. Toluidine blue staining for cell culture - (reply: 1)
  143. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  144. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  145. Annexin and propidium staining - (reply: 1)
  146. GUS histochemistry assay - No staining present (reply: 1)
  147. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  148. how to stain suspension culture? - (reply: 1)
  149. silver stain of immunoprecipitate - (reply: 2)
  150. NK cell staining - (reply: 4)
  151. Disposal of coomassie stain - (reply: 3)
  152. flagella stain - (reply: 1)
  153. Silver stained 2-d gel BLOB!!! - (reply: 1)
  154. microglia staining - protocol for microglia staining (reply: 1)
  155. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  156. what is the measurement of Oil red O staining - (reply: 3)
  157. Surface Staining and Methanol Fixation - (reply: 1)
  158. Live stain with propidium iodide - Incubation time & temperature? (reply: 3)
  159. immunofluorescence staining of sperm cells - (reply: 2)
  160. pre stained marker issues - (reply: 2)
  161. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  162. Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
  163. IHC staining of secreted proteins - (reply: 3)
  164. strange band in coomassie blue staining - (reply: 3)
  165. FBS Annexin staining - (reply: 1)
  166. siRNA staining? - (reply: 1)
  167. SDS-PAGE gel staining problem - (reply: 1)
  168. High background in phalloidin staining - (reply: 2)
  169. histological staining for plant cell wall components - (reply: 1)
  170. Antibody staining - (reply: 6)
  171. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
  172. Pre stained markers - (reply: 4)
  173. Staining spores - (reply: 1)
  174. DAPI/Hoechst vital staining - (reply: 3)
  175. Only borders stained in Silver!!! - (reply: 7)
  176. co staining problem: same primary species - (reply: 7)
  177. viability test: fixation AFTER propidium iodide staining? - (reply: 1)
  178. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  179. Staining of GLycoproteins in IEF - (reply: 4)
  180. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  181. collagen staining in cell culture - (reply: 5)
  182. CFSE staining of mycobacteria - (reply: 1)
  183. RNA elution after stains all staining - (reply: 3)
  184. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  185. Help needed with IF staining. - (reply: 2)
  186. vacuolation in MDCK cells - Vacuolation after staining (reply: 4)
  187. PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
  188. Staining of Intracellular Cytokines - (reply: 6)
  189. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  190. Th17 Differentiation and Staining - (reply: 18)
  191. Annexin V staining in combination with other antibodies - (reply: 4)
  192. DAB Peroxidase staining - (reply: 3)
  193. Fixation after or before staining? - (reply: 3)
  194. Preabsorption of Ab with Ag still leads to staining in ICC - (reply: 7)
  195. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  196. cell cycle analysis PI staining shift - (reply: 1)
  197. Ponceau Staining Question - (reply: 1)
  198. Intracellular stain of nuclear receptor - (reply: 6)
  199. sox-2 staining - (reply: 2)
  200. CFSE staining protocol - (reply: 14)
  201. What stains red in trypan blue - (reply: 2)
  202. PKH26 staining mesenchymal stem cells - toxicity (reply: 1)
  203. Beta Catenin immunofluorescent staining - (reply: 1)
  204. smeared silver stainning on acidic native page - how can I sovle smeared silver staining? (reply: 3)
  205. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
  206. YFP staining - (reply: 2)
  207. Problem with double staining for FACS - (reply: 1)
  208. Ponceau staining after primary and secondary? - (reply: 5)
  209. staining cells - (reply: 3)
  210. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  211. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  212. Purpose of methanol in the Blue Silver stain - (reply: 3)
  213. Bacterial Staining and Counting - (reply: 1)
  214. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  215. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  216. types of gel stains - (reply: 7)
  217. Etbr staining methods - (reply: 6)
  218. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  219. surface staining of THP-1 cell - (reply: 2)
  220. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  221. ER stained with Ab looks bad... - (reply: 3)
  222. HUVEC surface antigen staining - (reply: 1)
  223. Phosphoproteins staining - (reply: 2)
  224. apoptotic bodies- DAPI staining - (reply: 6)
  225. Integrin 1 staining - (reply: 2)
  226. Staining for neural crest derived cells?? - (reply: 2)
  227. problem to stain just one cell line in coculture - (reply: 4)
  228. ATPase staining for different Myosins - Anyone got a good protocol? (reply: 3)
  229. Is immunoblotting possible after the gel has been silver stained - (reply: 2)