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Staining Related Discussions
  1. Cytotoxicity of hoechst staining in overnight experiment - (reply: 1)
  2. Gram staining issues - (reply: 4)
  3. Use of fluorescent stain on IEF gels - (reply: 1)
  4. immunofluorescence staining protocol question - (reply: 1)
  5. protein staining with EtBr!! - (reply: 4)
  6. Syber green gel staining after electrophoresis - (reply: 4)
  7. Trypan Blue Staining - (reply: 1)
  8. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  9. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
  10. Use of Gram Stain kit with Reagents differentation - (reply: 2)
  11. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
  12. Staining SDS-PAGE gel with EtBr? - (reply: 6)
  13. polyA RNA staining for dot blot - (reply: 4)
  14. cell viability with PI staining - (reply: 2)
  15. silver stain destaining - (reply: 1)
  16. Spotted DAB staining! - (reply: 1)
  17. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
  18. Quantuitative staining of RNA and DNA? - (reply: 4)
  19. Karyotyping, Giemsa Staining - (reply: 4)
  20. How to differentiate Enterobacteriace on Gram Stain? - (reply: 3)
  21. Is possible that unknown culture came out negative for all the three staining te - (reply: 2)
  22. why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
  23. Quantification of the immunohistochemical staining slides - (reply: 4)
  24. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  25. In simple staining, what is the charge of the dye? - (reply: 1)
  26. PI staining cell cycle analysis - (reply: 1)
  27. Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
  28. 2 Photos - 2 Questions: Gram Stain - (reply: 4)
  29. H&E Staining help! - (reply: 1)
  30. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  31. Flow cytometry and immunofluorescent staining: help needed - (reply: 2)
  32. Low signal, weird background stains on blot - (reply: 5)
  33. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  34. Live cell sort DNA stain permeabilization - (reply: 1)
  35. Gram Staining: What can one see? - (reply: 3)
  36. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  37. Incomplete Blotting and staining of NC membrane - (reply: 7)
  38. Intracellular staining at 4C - HELP - (reply: 8)
  39. polyacrylamide gel with sybr green staining - (reply: 1)
  40. Intracellular staining of cytokines... crossreactivity? - (reply: 1)
  41. immunofluorescence - antibody choice for double staining - (reply: 2)
  42. Troubleshooting with silver staining - (reply: 3)
  43. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  44. Acid fast staining: need help - (reply: 5)
  45. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
  46. GFP staining with FACS - (reply: 1)
  47. co-IP stained with coomassie blue - (reply: 2)
  48. Losing cells during intracellular staining method! - (reply: 1)
  49. Smeared Bands after Ponceau Staining? - (reply: 3)
  50. coomassie blue stain - (reply: 1)
  51. How do you reduce crystal violet staining background on plates? - (reply: 1)
  52. Transwell migration assay (Boyden chambers) and DAPI staining - (reply: 1)
  53. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  54. Stain in electrophoresis is going from + to - - (reply: 3)
  55. Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot - (reply: 1)
  56. Looking for simple Papanicolaou (pap) staining protocol - (reply: 2)
  57. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
  58. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  59. Problem with Gamma H2AX foci staining - (reply: 5)
  60. Cell number issue - Nile Red staining - (reply: 3)
  61. Western blot and immunofluorescence staining - (reply: 9)
  62. Integrin B1 Staining in HT1080 cells - (reply: 1)
  63. Intracellular staining, isotype control not stable - (reply: 1)
  64. isoelctric gels hard to stain? - (reply: 2)
  65. Alizarin Red Staining on scaffolds... - (reply: 1)
  66. Mass-spec compatible silver stain - (reply: 2)
  67. viability/integrity stain of fungal cells - (reply: 5)
  68. NBT solution in X-Gal staining - (reply: 1)
  69. HELP-zebrafish antibody staining after day3 - (reply: 6)
  70. Please look at my gel picture, poor EthBr staining - (reply: 2)
  71. protein not stained by Coomassie Blue - (reply: 13)
  72. Flag Staining in U87 cells - (reply: 4)
  73. Cells detach during bodipy 493/503 staining - (reply: 1)
  74. Problem with sequential double IF staining with rabbit antibodies - (reply: 2)
  75. Distinguish murine and human cells by surface staining? - (reply: 1)
  76. Non-fluorescent live/dead stains for prokaryotes - (reply: 2)
  77. Live/Dead Staining of already Permeable Cells - (reply: 10)
  78. PVDF membrane silver staining protocol - (reply: 5)
  79. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
  80. Coomassie stain forzen and thawed gel? - (reply: 1)
  81. crystal violet staining - (reply: 5)
  82. non-specific staining using with Immunos - (reply: 3)
  83. CRYSTAL VIOLET STAINING - (reply: 3)
  84. can coommassie stained gel be western blot - (reply: 5)
  85. good humidified chambers for slide staining - (reply: 2)
  86. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  87. Phalloidin-Alexa Fluor 488 staining problem (huge background) - (reply: 1)
  88. Cell staining for fluorometric assay - (reply: 5)
  89. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  90. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  91. SDS-PAGE problem with staining yellow background - (reply: 3)
  92. What colour do sem*n stains glow under a black light? - (reply: 1)
  93. SDS-PAGE staining for digestion assay - (reply: 1)
  94. FACS staining of human macrophages derived from PBMCs - (reply: 3)
  95. quantify immunofluorescence staining - (reply: 1)
  96. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  97. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  98. DiI staining Fixation protocol - (reply: 1)
  99. Immunofluorescence Staining of Cells - (reply: 1)
  100. Immunofluorescence - unspecific staining - (reply: 9)
  101. Oil red O staining?? - (reply: 1)
  102. Nuclei staining background in collagen matrix - (reply: 1)
  103. Sphere staining protocol (agarose) - (reply: 3)
  104. DAB staining and GFP - (reply: 1)
  105. Actin staining - (reply: 1)
  106. Propidium iodide staining - (reply: 1)
  107. Trypan Blue Staining - (reply: 3)
  108. problem in making a proper smear for nile blue staining of an unknown yeast - (reply: 2)
  109. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  110. RNA extraction from blood stain - (reply: 1)
  111. Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati - (reply: 1)
  112. Searching for a live cell staining method - (reply: 1)
  113. ANNEXIN V staining for FLOW - (reply: 3)
  114. What positive control for intracellular staining? - (reply: 1)
  115. Simultaneous Double Staining in paraffin tissues - (reply: 2)
  116. Which stain is frequently used to identify Mycobacterium - (reply: 1)
  117. Forgetting iodine when gram staining - (reply: 1)
  118. intracellular cytokine staining - (reply: 1)
  119. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  120. X-gal staining and IHC against b-gal - (reply: 7)
  121. mass compatible silver staining - (reply: 1)
  122. Staining Cells in Suspension - (reply: 4)
  123. Bradford reagent for staining gels? - (reply: 1)
  124. Single stain control for 293 cell line - (reply: 4)
  125. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  126. Intracellular staining for IL-21 - (reply: 1)
  127. Annexin-V staining - (reply: 1)
  128. apoptosis assay - immunofluoresence staining (reply: 5)
  129. Pre-cutting before staining - How long before staining can samples be cut? (reply: 1)
  130. silver staining of ip sample - (reply: 1)
  131. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
  132. GFP cells intracellular staining - (reply: 1)
  133. gelatin staining with coomassie: how do I make it work? - (reply: 3)
  134. X-gal stained tissue for IFC? - Will this work? (reply: 2)
  135. ICC: exotic problem with staining, vGlut1 ans synapsin-1 - (reply: 2)
  136. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  137. Detection of Apoptosis by Annexin V PI staining - (reply: 1)
  138. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  139. Histochemical stain specific for transthyretin (prealbumin)??! - Anyone aware if there s such a thing? (reply: 2)
  140. Fixing cells before staining of surface markers - (reply: 1)
  141. TLC Visualizing Stains - (reply: 1)
  142. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  143. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  144. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  145. Negative control is positive for IF staining - (reply: 3)
  146. intracelluar staining without killing cells - (reply: 3)
  147. Hoechst Stain does it stain dead cells - (reply: 3)
  148. Diastase or Amylase Digestion - Negative for PAS stain (reply: 4)
  149. detection of mycoplasma with hoechst staining - (reply: 1)
  150. short question - FDG staining (reply: 2)
  151. Flow cytometry-frozen PBMCs - Surface and intracellular staining (reply: 5)
  152. Basic questions about PI staining in Flowcyto - (reply: 7)
  153. T helper differentiation and staining - Trouble with intracellular staining (reply: 6)
  154. Help Stained after blocking and won't unstain!!! - (reply: 1)
  155. immunochemical staining problem - i'm struggling to resolve this problem (reply: 1)
  156. Help-IHC nuclear staining - (reply: 2)
  157. imunofluerecence staining with Alexa 568 dye - interesting (reply: 1)
  158. No protein spots in 2D gel - Silver staining! - (reply: 17)
  159. RNA extraction from stained cells - (reply: 2)
  160. Ponceau Red Staining Problem - (reply: 2)
  161. How to stain for oxidative stress - (reply: 1)
  162. Roles of staining soluton components!!! - (reply: 6)
  163. Toluidine blue staining for cell culture - (reply: 1)
  164. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  165. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  166. Annexin and propidium staining - (reply: 1)
  167. GUS histochemistry assay - No staining present (reply: 1)
  168. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  169. how to stain suspension culture? - (reply: 1)
  170. silver stain of immunoprecipitate - (reply: 2)
  171. NK cell staining - (reply: 4)
  172. Disposal of coomassie stain - (reply: 3)
  173. flagella stain - (reply: 1)
  174. Silver stained 2-d gel BLOB!!! - (reply: 1)
  175. microglia staining - protocol for microglia staining (reply: 1)
  176. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  177. what is the measurement of Oil red O staining - (reply: 3)
  178. Surface Staining and Methanol Fixation - (reply: 1)
  179. Live stain with propidium iodide - Incubation time & temperature? (reply: 3)
  180. immunofluorescence staining of sperm cells - (reply: 2)
  181. pre stained marker issues - (reply: 2)
  182. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  183. Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
  184. IHC staining of secreted proteins - (reply: 3)
  185. strange band in coomassie blue staining - (reply: 3)
  186. FBS Annexin staining - (reply: 1)
  187. siRNA staining? - (reply: 1)
  188. SDS-PAGE gel staining problem - (reply: 1)
  189. High background in phalloidin staining - (reply: 2)
  190. histological staining for plant cell wall components - (reply: 1)
  191. Antibody staining - (reply: 6)
  192. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
  193. Pre stained markers - (reply: 4)
  194. Staining spores - (reply: 1)
  195. DAPI/Hoechst vital staining - (reply: 3)
  196. Only borders stained in Silver!!! - (reply: 7)
  197. co staining problem: same primary species - (reply: 7)
  198. viability test: fixation AFTER propidium iodide staining? - (reply: 1)
  199. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  200. Staining of GLycoproteins in IEF - (reply: 4)
  201. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  202. collagen staining in cell culture - (reply: 5)
  203. CFSE staining of mycobacteria - (reply: 1)
  204. RNA elution after stains all staining - (reply: 3)
  205. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  206. Help needed with IF staining. - (reply: 2)
  207. vacuolation in MDCK cells - Vacuolation after staining (reply: 4)
  208. PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
  209. Staining of Intracellular Cytokines - (reply: 6)
  210. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  211. Th17 Differentiation and Staining - (reply: 18)
  212. Annexin V staining in combination with other antibodies - (reply: 4)
  213. DAB Peroxidase staining - (reply: 3)
  214. Fixation after or before staining? - (reply: 3)
  215. Preabsorption of Ab with Ag still leads to staining in ICC - (reply: 7)
  216. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  217. cell cycle analysis PI staining shift - (reply: 1)
  218. Ponceau Staining Question - (reply: 1)
  219. Intracellular stain of nuclear receptor - (reply: 6)
  220. sox-2 staining - (reply: 2)
  221. CFSE staining protocol - (reply: 14)
  222. What stains red in trypan blue - (reply: 2)
  223. PKH26 staining mesenchymal stem cells - toxicity (reply: 1)
  224. Beta Catenin immunofluorescent staining - (reply: 1)
  225. smeared silver stainning on acidic native page - how can I sovle smeared silver staining? (reply: 3)
  226. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
  227. YFP staining - (reply: 2)
  228. Problem with double staining for FACS - (reply: 1)
  229. Ponceau staining after primary and secondary? - (reply: 5)
  230. staining cells - (reply: 3)
  231. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  232. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  233. Purpose of methanol in the Blue Silver stain - (reply: 3)
  234. Bacterial Staining and Counting - (reply: 1)
  235. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  236. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  237. types of gel stains - (reply: 7)
  238. Etbr staining methods - (reply: 6)
  239. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  240. surface staining of THP-1 cell - (reply: 2)
  241. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  242. ER stained with Ab looks bad... - (reply: 3)
  243. HUVEC surface antigen staining - (reply: 1)
  244. Phosphoproteins staining - (reply: 2)
  245. apoptotic bodies- DAPI staining - (reply: 6)
  246. Integrin 1 staining - (reply: 2)
  247. Staining for neural crest derived cells?? - (reply: 2)
  248. problem to stain just one cell line in coculture - (reply: 4)
  249. ATPase staining for different Myosins - Anyone got a good protocol? (reply: 3)
  250. Is immunoblotting possible after the gel has been silver stained - (reply: 2)