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Staining Related Discussions
  1. unusual FITC background staining - (reply: 2)
  2. lower part of the gel was not stained - (reply: 7)
  3. Can microglia stain positive for phagocytosed material? - (reply: 2)
  4. Cytotoxicity of hoechst staining in overnight experiment - (reply: 1)
  5. Gram staining issues - (reply: 4)
  6. Use of fluorescent stain on IEF gels - (reply: 1)
  7. Annexin PI staining - (reply: 1)
  8. immunofluorescence staining protocol question - (reply: 1)
  9. protein staining with EtBr!! - (reply: 4)
  10. Syber green gel staining after electrophoresis - (reply: 4)
  11. Trypan Blue Staining - (reply: 1)
  12. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  13. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
  14. Use of Gram Stain kit with Reagents differentation - (reply: 2)
  15. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
  16. Staining SDS-PAGE gel with EtBr? - (reply: 6)
  17. polyA RNA staining for dot blot - (reply: 4)
  18. cell viability with PI staining - (reply: 2)
  19. silver stain destaining - (reply: 1)
  20. Spotted DAB staining! - (reply: 1)
  21. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
  22. Quantuitative staining of RNA and DNA? - (reply: 4)
  23. Karyotyping, Giemsa Staining - (reply: 4)
  24. How to differentiate Enterobacteriace on Gram Stain? - (reply: 3)
  25. Is possible that unknown culture came out negative for all the three staining te - (reply: 2)
  26. why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
  27. Quantification of the immunohistochemical staining slides - (reply: 4)
  28. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  29. In simple staining, what is the charge of the dye? - (reply: 1)
  30. PI staining cell cycle analysis - (reply: 1)
  31. Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
  32. 2 Photos - 2 Questions: Gram Stain - (reply: 4)
  33. H&E Staining help! - (reply: 1)
  34. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  35. Flow cytometry and immunofluorescent staining: help needed - (reply: 2)
  36. Low signal, weird background stains on blot - (reply: 5)
  37. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  38. Live cell sort DNA stain permeabilization - (reply: 1)
  39. Gram Staining: What can one see? - (reply: 3)
  40. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  41. Incomplete Blotting and staining of NC membrane - (reply: 7)
  42. Intracellular staining at 4C - HELP - (reply: 8)
  43. polyacrylamide gel with sybr green staining - (reply: 1)
  44. Intracellular staining of cytokines... crossreactivity? - (reply: 1)
  45. immunofluorescence - antibody choice for double staining - (reply: 2)
  46. Troubleshooting with silver staining - (reply: 3)
  47. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  48. Acid fast staining: need help - (reply: 5)
  49. DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
  50. GFP staining with FACS - (reply: 1)
  51. co-IP stained with coomassie blue - (reply: 2)
  52. Losing cells during intracellular staining method! - (reply: 1)
  53. Smeared Bands after Ponceau Staining? - (reply: 3)
  54. coomassie blue stain - (reply: 1)
  55. How do you reduce crystal violet staining background on plates? - (reply: 1)
  56. Transwell migration assay (Boyden chambers) and DAPI staining - (reply: 1)
  57. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  58. Stain in electrophoresis is going from + to - - (reply: 3)
  59. Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot - (reply: 1)
  60. Looking for simple Papanicolaou (pap) staining protocol - (reply: 2)
  61. Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
  62. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  63. Problem with Gamma H2AX foci staining - (reply: 5)
  64. Cell number issue - Nile Red staining - (reply: 3)
  65. Western blot and immunofluorescence staining - (reply: 9)
  66. Integrin B1 Staining in HT1080 cells - (reply: 1)
  67. Intracellular staining, isotype control not stable - (reply: 1)
  68. isoelctric gels hard to stain? - (reply: 2)
  69. Alizarin Red Staining on scaffolds... - (reply: 1)
  70. Mass-spec compatible silver stain - (reply: 2)
  71. viability/integrity stain of fungal cells - (reply: 5)
  72. NBT solution in X-Gal staining - (reply: 1)
  73. HELP-zebrafish antibody staining after day3 - (reply: 6)
  74. Please look at my gel picture, poor EthBr staining - (reply: 2)
  75. protein not stained by Coomassie Blue - (reply: 13)
  76. Flag Staining in U87 cells - (reply: 4)
  77. Cells detach during bodipy 493/503 staining - (reply: 1)
  78. Problem with sequential double IF staining with rabbit antibodies - (reply: 2)
  79. Distinguish murine and human cells by surface staining? - (reply: 1)
  80. Non-fluorescent live/dead stains for prokaryotes - (reply: 2)
  81. Live/Dead Staining of already Permeable Cells - (reply: 10)
  82. PVDF membrane silver staining protocol - (reply: 5)
  83. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
  84. Coomassie stain forzen and thawed gel? - (reply: 1)
  85. crystal violet staining - (reply: 6)
  86. non-specific staining using with Immunos - (reply: 3)
  87. CRYSTAL VIOLET STAINING - (reply: 3)
  88. can coommassie stained gel be western blot - (reply: 5)
  89. good humidified chambers for slide staining - (reply: 2)
  90. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  91. Phalloidin-Alexa Fluor 488 staining problem (huge background) - (reply: 1)
  92. Cell staining for fluorometric assay - (reply: 5)
  93. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  94. staining artifacts in polyacrylamide gel ?? - (reply: 2)
  95. SDS-PAGE problem with staining yellow background - (reply: 3)
  96. What colour do sem*n stains glow under a black light? - (reply: 1)
  97. SDS-PAGE staining for digestion assay - (reply: 1)
  98. FACS staining of human macrophages derived from PBMCs - (reply: 3)
  99. quantify immunofluorescence staining - (reply: 1)
  100. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  101. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  102. DiI staining Fixation protocol - (reply: 1)
  103. Immunofluorescence Staining of Cells - (reply: 1)
  104. Immunofluorescence - unspecific staining - (reply: 9)
  105. Oil red O staining?? - (reply: 1)
  106. Nuclei staining background in collagen matrix - (reply: 1)
  107. Sphere staining protocol (agarose) - (reply: 3)
  108. DAB staining and GFP - (reply: 1)
  109. Actin staining - (reply: 1)
  110. Propidium iodide staining - (reply: 1)
  111. Trypan Blue Staining - (reply: 3)
  112. problem in making a proper smear for nile blue staining of an unknown yeast - (reply: 2)
  113. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  114. RNA extraction from blood stain - (reply: 1)
  115. Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati - (reply: 1)
  116. Searching for a live cell staining method - (reply: 1)
  117. ANNEXIN V staining for FLOW - (reply: 3)
  118. What positive control for intracellular staining? - (reply: 1)
  119. Simultaneous Double Staining in paraffin tissues - (reply: 2)
  120. Which stain is frequently used to identify Mycobacterium - (reply: 1)
  121. Forgetting iodine when gram staining - (reply: 1)
  122. intracellular cytokine staining - (reply: 1)
  123. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  124. X-gal staining and IHC against b-gal - (reply: 7)
  125. mass compatible silver staining - (reply: 1)
  126. Staining Cells in Suspension - (reply: 4)
  127. Bradford reagent for staining gels? - (reply: 1)
  128. Single stain control for 293 cell line - (reply: 4)
  129. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  130. Intracellular staining for IL-21 - (reply: 1)
  131. Annexin-V staining - (reply: 1)
  132. apoptosis assay - immunofluoresence staining (reply: 5)
  133. Pre-cutting before staining - How long before staining can samples be cut? (reply: 1)
  134. silver staining of ip sample - (reply: 1)
  135. immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
  136. GFP cells intracellular staining - (reply: 1)
  137. gelatin staining with coomassie: how do I make it work? - (reply: 3)
  138. X-gal stained tissue for IFC? - Will this work? (reply: 2)
  139. ICC: exotic problem with staining, vGlut1 ans synapsin-1 - (reply: 2)
  140. NuPAGE Gel get smaller after staining o/n - (reply: 1)
  141. Detection of Apoptosis by Annexin V PI staining - (reply: 1)
  142. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  143. Histochemical stain specific for transthyretin (prealbumin)??! - Anyone aware if there s such a thing? (reply: 2)
  144. Fixing cells before staining of surface markers - (reply: 1)
  145. TLC Visualizing Stains - (reply: 1)
  146. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  147. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  148. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  149. Negative control is positive for IF staining - (reply: 3)
  150. intracelluar staining without killing cells - (reply: 3)
  151. Hoechst Stain does it stain dead cells - (reply: 3)
  152. Diastase or Amylase Digestion - Negative for PAS stain (reply: 4)
  153. detection of mycoplasma with hoechst staining - (reply: 1)
  154. short question - FDG staining (reply: 2)
  155. Flow cytometry-frozen PBMCs - Surface and intracellular staining (reply: 5)
  156. Basic questions about PI staining in Flowcyto - (reply: 7)
  157. T helper differentiation and staining - Trouble with intracellular staining (reply: 6)
  158. Help Stained after blocking and won't unstain!!! - (reply: 1)
  159. immunochemical staining problem - i'm struggling to resolve this problem (reply: 1)
  160. Help-IHC nuclear staining - (reply: 2)
  161. imunofluerecence staining with Alexa 568 dye - interesting (reply: 1)
  162. No protein spots in 2D gel - Silver staining! - (reply: 17)
  163. RNA extraction from stained cells - (reply: 2)
  164. Ponceau Red Staining Problem - (reply: 2)
  165. How to stain for oxidative stress - (reply: 1)
  166. Roles of staining soluton components!!! - (reply: 6)
  167. Toluidine blue staining for cell culture - (reply: 1)
  168. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  169. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
  170. Annexin and propidium staining - (reply: 1)
  171. GUS histochemistry assay - No staining present (reply: 1)
  172. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  173. how to stain suspension culture? - (reply: 1)
  174. silver stain of immunoprecipitate - (reply: 2)
  175. NK cell staining - (reply: 4)
  176. Disposal of coomassie stain - (reply: 3)
  177. flagella stain - (reply: 1)
  178. Silver stained 2-d gel BLOB!!! - (reply: 1)
  179. microglia staining - protocol for microglia staining (reply: 1)
  180. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  181. what is the measurement of Oil red O staining - (reply: 3)
  182. Surface Staining and Methanol Fixation - (reply: 1)
  183. Live stain with propidium iodide - Incubation time & temperature? (reply: 3)
  184. immunofluorescence staining of sperm cells - (reply: 2)
  185. pre stained marker issues - (reply: 2)
  186. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  187. Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
  188. IHC staining of secreted proteins - (reply: 3)
  189. strange band in coomassie blue staining - (reply: 3)
  190. FBS Annexin staining - (reply: 1)
  191. siRNA staining? - (reply: 1)
  192. SDS-PAGE gel staining problem - (reply: 1)
  193. High background in phalloidin staining - (reply: 2)
  194. histological staining for plant cell wall components - (reply: 1)
  195. Antibody staining - (reply: 6)
  196. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
  197. Pre stained markers - (reply: 4)
  198. Staining spores - (reply: 1)
  199. DAPI/Hoechst vital staining - (reply: 3)
  200. Only borders stained in Silver!!! - (reply: 7)
  201. co staining problem: same primary species - (reply: 7)
  202. viability test: fixation AFTER propidium iodide staining? - (reply: 1)
  203. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  204. Staining of GLycoproteins in IEF - (reply: 4)
  205. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  206. collagen staining in cell culture - (reply: 5)
  207. CFSE staining of mycobacteria - (reply: 1)
  208. RNA elution after stains all staining - (reply: 3)
  209. Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
  210. Help needed with IF staining. - (reply: 2)
  211. vacuolation in MDCK cells - Vacuolation after staining (reply: 4)
  212. PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
  213. Staining of Intracellular Cytokines - (reply: 6)
  214. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  215. Th17 Differentiation and Staining - (reply: 18)
  216. Annexin V staining in combination with other antibodies - (reply: 4)
  217. DAB Peroxidase staining - (reply: 3)
  218. Fixation after or before staining? - (reply: 3)
  219. Preabsorption of Ab with Ag still leads to staining in ICC - (reply: 7)
  220. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  221. cell cycle analysis PI staining shift - (reply: 1)
  222. Ponceau Staining Question - (reply: 1)
  223. Intracellular stain of nuclear receptor - (reply: 6)
  224. sox-2 staining - (reply: 2)
  225. CFSE staining protocol - (reply: 14)
  226. What stains red in trypan blue - (reply: 2)
  227. PKH26 staining mesenchymal stem cells - toxicity (reply: 1)
  228. Beta Catenin immunofluorescent staining - (reply: 1)
  229. smeared silver stainning on acidic native page - how can I sovle smeared silver staining? (reply: 3)
  230. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
  231. YFP staining - (reply: 2)
  232. Problem with double staining for FACS - (reply: 1)
  233. Ponceau staining after primary and secondary? - (reply: 5)
  234. staining cells - (reply: 3)
  235. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  236. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  237. Purpose of methanol in the Blue Silver stain - (reply: 3)
  238. Bacterial Staining and Counting - (reply: 1)
  239. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  240. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  241. types of gel stains - (reply: 7)
  242. Etbr staining methods - (reply: 6)
  243. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
  244. surface staining of THP-1 cell - (reply: 2)
  245. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  246. ER stained with Ab looks bad... - (reply: 3)
  247. HUVEC surface antigen staining - (reply: 1)
  248. Phosphoproteins staining - (reply: 2)
  249. apoptotic bodies- DAPI staining - (reply: 6)
  250. Integrin 1 staining - (reply: 2)
  251. Staining for neural crest derived cells?? - (reply: 2)
  252. problem to stain just one cell line in coculture - (reply: 4)
  253. ATPase staining for different Myosins - Anyone got a good protocol? (reply: 3)
  254. Is immunoblotting possible after the gel has been silver stained - (reply: 2)