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Help Stained after blocking and won't unstain!!! - (Sep/02/2010 )

I made a dumb mistake. I accidently stained my membrane with Ponceau S after staining for an hour!!! My whole membrane turned red! And will not unstain. Any advice??!!

-Stuck with Ligation-

Unfortunately there is little you can do about this (presuming you mean that you blocked for 1 hour and then Ponceau), other than lots of washes - try in blocking solution - it has protein so it should remove some of the stain at least.