Surface Staining and Methanol Fixation - (Mar/24/2010 )
Is it feasible to stain for a surface antigen, then fix the cells with methanol and subsequently stain for an intracellular antigen?
I have done with commercial fix & perm solutions, but never with methanol and in the datasheet of the intracellular antibody they say cells should be fixed with this
-canotto-
Ethanol and methanol fixation (for DNA content?) is not compartible with surface staining.
-Denis Baev-