DAPI/Hoechst vital staining - (Nov/02/2009 )

Hi there;

I wanna stain GFP transfected human cells with DAPI or Hoechst. My problem is that every fixation kills my GFP in the cells. Does anyone know a good protocol for vital staining of the nucleus without any fixation of the cell.


Thx Neo

neopeg on Nov 2 2009, 07:31 AM said:

It's an extra few steps, but what about using an antibody for GFP?

Fixing shouldn't kill the GFP, it is pretty stable... have you tried 2% cold paraformaldehyde?

DRAQ 5
http://www.biostatus.com/product/draq5/

however, I agree with above commentary. What are your fixation conditions?