GFP staining with FACS - (Jul/19/2013 )
i am working on a very rare subset of mouse DC. Now I got an EGFP-mouse for my protein of interest, but could not detect GFP pos. cells in mouse DC of skindraining LN. The protein is probably intracellularly, but lysis did not help. I only expect very low numbers of
cells - but compared to my non-EGFP controll cells, I could not detect any...
Does anyone have experience with using an anti-GFP antibody (which clone?) in FACS to enhance the signal? Does that make sense? I assumed the GFP signal to be strong enough - if present. I am wrong?
thanks a lot!
Anti-gfp ab290 raised in rabbit is great.