H&E Staining help! - (Mar/05/2014 )

Hi guys,

Recently I've been staining my mouse hearts with H&E. Although the staining works, my nuclei seems too faint, and my eosin is extremely pink. I've tried decreasing the time for the Eosin from 4 minutes to 2 minutes, and increasing the time for Hematoxylin from 5 minutes to 7 minutes, but the results are the same. I am using Mayer's H&E from Fisher to stain these slides. The fixation time is one day in formaldehyde, and the sections are 6uM thick. Any experts out there have any idea?

Thanks a bunch!

Hi:

Try with Harry´s Haematoxylin 5 minutes, wash 5 minutes in running tap water , counterstain 5 seconds with 0.5% HCL in water, wash 5 minutes in running tap water and stain only 20 seconds in alcoholic Eosin.

good luck