to increase the sensitivity of SDS-PAGE silver staining?? - (May/29/2009 )

hi

Do any one is familiar with methods to improve sensitivity of silver staining technique used for SDS-PAGE??

thanks a lot

one thing that we have done is stain twice (with or, mostly, without a destaining step). but the background can get very dark.

with the silver stain method we use (modified, by us, merril) we still get a translucent (amber) background.