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to increase the sensitivity of SDS-PAGE silver staining?? - (May/28/2009 )

hi

Do any one is familiar with methods to improve sensitivity of silver staining technique used for SDS-PAGE??

thanks a lot

-rick112-

one thing that we have done is stain twice (with or, mostly, without a destaining step). but the background can get very dark.

with the silver stain method we use (modified, by us, merril) we still get a translucent (amber) background.

-mdfenko-