Ponceau Staining Question - (Jul/16/2009 )
Quick ponceau question. I was running behind yesterday when i started my western and didn't have a chance to ponceau stain it before i did my blocking step overnight. Is there any chance I can still stain it after I strip the membrane of my first set of primary/secondary antibodies and get an idea about the protein per lane or will the blocking obscure that?
blocking will obscure. Ponceau binds to protein and you use protein for blocking...