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sox-2 staining - (Jul/15/2009 )

Hi,

I am trying to staing mouse brain sections for sox-2 (frozen, FIXED WITH PFA 4%).
The problem is that I get a lot of positive stainig - not only in the DG and SVZ, but also all over the brain.
It doesnt look like backgroung. Did anyone tryied to use sox-2 antibody ? do you expect to see so many positive cells?

Thanks,
Rona

-ronal-

Hi,
I have never tried Sox2 on the brain. However, it seems that you are not alone (click here).
Cheers,
Minna
Attached File

-Minna-

ronal on Jul 15 2009, 11:14 PM said:

Hi,

I am trying to staing mouse brain sections for sox-2 (frozen, FIXED WITH PFA 4%).
The problem is that I get a lot of positive stainig - not only in the DG and SVZ, but also all over the brain.
It doesnt look like backgroung. Did anyone tryied to use sox-2 antibody ? do you expect to see so many positive cells?

Thanks,
Rona



I have only done Sox2 in embryonic stem cells, neural stem cells and in E12-E18 rat sections. The staining should be very nuclear and it should only be in areas of neural stem cells (so it should not be everywhere). I an antibody from R and D Systems, Cat # MAB2018 and have never had problems. I have seen Sox2 staining everywhere with other antibodies. GL

-gradstem-