Problems of beta-actin - (Sep/11/2014 )
This is beta-actin. I got multi-signals about this. I am sure i didn't move the film when it was exposed to signal.
Was too much sample loaded?
The same samples were used many times. It is no problem when i use it first time.
Was the store method wrong? I store sample in -20°C.
Thanks a lot.
Non-spcific antibody reactions, use a shorter secondary incubation (1h or less) and try using a higher dilution of primary.