Problems of beta-actin - (Sep/11/2014 )

Hi, everyone

This is beta-actin. I got multi-signals about this. I am sure i didn't move the film when it was exposed to signal.

Was too much sample loaded? 

The same samples were used many times. It is no problem when i use it first time.

Was the store method wrong? I store sample in -20°C.

Thanks a lot.

Non-spcific antibody reactions, use a shorter secondary incubation (1h or less) and try using a higher dilution of primary.