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Problems of beta-actin - (Sep/11/2014 )

Hi, everyone


This is beta-actin. I got multi-signals about this. I am sure i didn't move the film when it was exposed to signal.


Attached Image


Was too much sample loaded? 


The same samples were used many times. It is no problem when i use it first time.


Was the store method wrong? I store sample in -20°C.



Thanks a lot.


-cell 293-

Non-spcific antibody reactions, use a shorter secondary incubation (1h or less) and try using a higher dilution of primary.