Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Stripping temperature - have no 55C water bath (Apr/04/2010 )

Hi all,
Our lab does not have a working water bath now. And I wonder if I can use room temperature to strip my WB membrane? or 37C?

I use home made stripping buffer:

62.5mmol/l Tris PH6.7
100mmol/l beta-mercaptoethanol



I'd try it at 37C if that's all you have. But if you have a floor shaker incubator that you can adjust the temp on somewhere in your building that would be good too.

If you're desperate try making your own water bath, but I don't know how long you strip for. I almost never strip, just run another gel!