What kind of post-translational modification do you think are these？ - (Mar/17/2010 )
Hey guys,my interested protein is cell cycle dependent,so I want to explain why would that expression pattern was achived?I think I find some hints of post-translational modification ,but I'm not sure.
Do you have any idea on how to explain the cell cycle dependent expression pattern of a protein?I mean how to plan some experienment to explain the mechenism.
Do you know any experienments to prove the existence of some post-translational modification,say ubiquitionation.
As for the grey picture,why would the potential modified binds be on the left and right edge of the lanes?And I have noticed other people's so called ubiquitination modification were also on the left and right edge of the lanes.
AllenChiu on Mar 17 2010, 08:24 PM said:
They aren't really on the edges of the lanes, it is just that the bands produced are brighter (in your pictures at least) so they spread a bit more on film, so that the bands appear wider than the other, fainter, bands. Often post translational modification causes a size shift in the protein (gain of mass) which is usually how they are detected.
bob1 on Mar 19 2010, 07:32 AM said:
AllenChiu on Mar 17 2010, 08:24 PM said:
They aren't really on the edges of the lanes, it is just that the bands produced are brighter (in your pictures at least) so they spread a bit more on film, so that the bands appear wider than the other, fainter, bands. Often post translational modification causes a size shift in the protein (gain of mass) which is usually how they are detected.
Thank you very much！