BN-PAGE possible with Tris-glycine - (Jul/16/2010 )
Hi all, just wondering if anybody knows a protocol for BN-PAGE using Tris-glycine system? All the protocols and papers i read on BN-Page uses Bis-Tris tricine system with pH ~7. If I use a tris-glycine system, should i adjust my pH to 7 as well? Thanks
use the buffer system called for by the protocol.
unless you want to develop your own.