how long is too long for ELISA develping? - (Oct/03/2007 )

My last few took overnight...are these result bogus? Thanks!

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Off the top of my head, without any experimental details, I'd say yes! What substrate and enzyme are you using? Actually, what is your protocol?

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may run standards in parallel with your samples to calibrate your signals;

signals should run in the linear phase of calibration

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sorry...don't know how i posted this topic 2x...will post my protocol and see what you think...thanks!!

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