how long is too long for ELISA develping? - (Oct/03/2007 )
My last few took overnight...are these result bogus? Thanks!
Off the top of my head, without any experimental details, I'd say yes! What substrate and enzyme are you using? Actually, what is your protocol?
may run standards in parallel with your samples to calibrate your signals;
signals should run in the linear phase of calibration
sorry...don't know how i posted this topic 2x...will post my protocol and see what you think...thanks!!