ELISA Plates - comparison of OD (Aug/26/2005 )

I was using cell culture grade ELISA plates of Corning for sandwich ELISA and my results were not appreciable especially for a specific serotype of Poliovirus. Then i started using Maxisorp NUNC plates and the O.D improved quite well. Can any one guide me or atleast give any scientific reason for that.
I need help in this regard thats why I chose this forum.
Regards

Hi,

Even I had the same problem. When I used cell culture treated 96 well plate for ELISA, I didn't get any readings. When I talked with an expert he told me that cell culture treated well plates doesn't coat antibody/protein on their surface.

May be it is something to do with hydrophobic interactions that bind the coating antibody with the polystyrene surface.

I would like to know if anybody faced the same problem

Good Luck

Afternoon all,

It's all down to how well the plate can adsorb the antibodies. NUNC maxisorb are pretty good, as you know, another good one is Costar's catalogue number 2592 (I think).

If you are really having problems, some suppliers have pre-coated plates, eg, goat anti-mouse. These are usually pretty consistent:

eg http://www.rndsystems.com/pdf/cp001.pdf

Obviously you need to be using a mouse primary for this though...

Most people find it surprising how much difference using different plates makes, same with BSA...

Cheers.

In producing plastic coating surfaces another factor is "releasing agents" which will effect ab adsorption.