Max. usage for one ELISA plate - (Oct/14/2008 )
Hi, this is abit silly question, but i just feel curious, how many patient samples can be run by using one ELISA kit? Most of the ELISA kits come with only 96 tests, and we need to do dilution for it, then how can it be 96 test = 1 well for 1 test for 1 patient sample? How if i want to get triplicate result for one patient sample? Help me, i just cannot imagine and i'm totally new in this O.o"
If you do titration for 7 times per sample, then you need 8 wells (one column of the plate)
Then, you need to do triplicate per sample, then 3 column.
So, 24wells per patient sample.
This means 4 patients.
I hope this may help.
use a electronic multichannel pipette for better ELISA results
Is there any difference between the ELISA kit for diagnostic used and research used?
Is there any difference between the ELISA kit for diagnostic used and research used?
yes...
I think kit for diagnostic used are more expensive and have more stringent quality control.
The price isn't the difference as isn't the quality control , the FDA approval is so there is a garantee that the diagnostic kit has gone through a FDA evaluation study with succes.
The costs for the production of diagnostic kit are higher but this will be compensated bij the number of kits sold.
I don't know for sure but i expect that the production for both type of kits bij the same producer will get the same qualitycontrol.
Many different questions in this section:
1. 96 wells one well per point. Just calculate how many standard points you will be running, number of samples and number of replicates. The major question, no one picked up on...is if the plate is solid or removable wells. If it is one solid plate you have to use the whole thing or somehow isolate the wells you will be using. Again...96 individule test points (samples and standards/calibrators)
2. Research kit vs clinical kit. It is NOT the price. The clinical kit is FDA approved for reporting patient results. It has been tested for performance to an existing fda approved product including sensitivity, specificity, linearity, interference, cross reactivity, and stability. (all this info is supplied to FDA...and is available to user/customer)
Research kit is just that "research only" can not be used for patient diagnosis...reporting results.
Research kits can cost more than the clinical kits and vice versa. Most clinical elisa kits are actually components used on automated instruments except for the relatively few analytes run semi-manually.
Hopes this clarifies information.
1. 96 wells one well per point. Just calculate how many standard points you will be running, number of samples and number of replicates. The major question, no one picked up on...is if the plate is solid or removable wells. If it is one solid plate you have to use the whole thing or somehow isolate the wells you will be using. Again...96 individule test points (samples and standards/calibrators)
2. Research kit vs clinical kit. It is NOT the price. The clinical kit is FDA approved for reporting patient results. It has been tested for performance to an existing fda approved product including sensitivity, specificity, linearity, interference, cross reactivity, and stability. (all this info is supplied to FDA...and is available to user/customer)
Research kit is just that "research only" can not be used for patient diagnosis...reporting results.
Research kits can cost more than the clinical kits and vice versa. Most clinical elisa kits are actually components used on automated instruments except for the relatively few analytes run semi-manually.
Hopes this clarifies information.
Thanks for these useful info. I'll be doing the association studies of genetic variants with biochemical marker level of heart disease patients. So if in that case, what kit i should look for? Clinical? or Research kit is enough?
Thanks
Your genetic kit is probably a research kit for a biomarker gene etc (I am not molecular biologist). This kit or test or one you are developing has not been conclusively proven to predict heart disease etc and is a research only kit.
Along with this research kit...I would suggest you run tests for cardiac inflammatory disease such as BNP, pro BNP etc...for actual heart attack, ischemia, conduct myoglobin and troponin I or troponin T tests. All of these are available on automated analysers (Siemens, Roche, Abbott) and there are a few manual Elisa kits as well.
Your focus would be to compare your research findings with some type of recognized reference (Patient results). In a nutshell...R&D biomarker vs. FDA approved kit (or clinical results)
I hope I have been of some help.