PGE2 ELISA - (Feb/07/2008 )
I am using R&D systems PGE2 ELISA, and am having problems with variable results. For instance my 0 minute negative control comes up as <dectection limit 2 times then has 300pg/ml in the next assay, although the cells are literally 'shown' the media before it is taken off. Does anyone know of a reason why this could be happening?
Could you please tell me more about your assay? which buffer?
I am using EIA kit from Oxford Biomedical Research, it is working well.