ELISA standards. - (Jul/28/2008 )
I am using an IL-10 ELISA kit from R&D. I was wondering whether I should run standards each time I run the assay or could I just make do with running the std for the Standard curve just once.Th e instruction manual says I have to repeat all the stds each time I run the assay.These kits are quite exp..So i wanted to know some saving tips.
Very bad idea to miss out standards! If you really must change the protocol, you could reduce the number of points in the standard curve. If you are really confident of your skills, and if the assay is to find out where the IL-10 is in a purification, you could do the samples as singletons, rather than duplicates, but you would have to be very very sure of your technique before you do that. No responsibility if it goes pear-shaped...
Single points would be next option. If you can not do that then run the 0 point, midpoint and one point above and below this..... as close as possible to the inflection points
I agree with the previous two responses, but you should never drop your standards. at the very least you can purchase recombinant IL-10 from R&D separately and make your own stds.