how long is too long for ELISA develping? - (Oct/03/2007 )
My last few took overnight...are these result bogus? Thanks!
-immuno9-
QUOTE (immuno9 @ Oct 3 2007, 06:19 PM)
My last few took overnight...are these result bogus? Thanks!
run standards in parallel with your samples to calibrate your signals;
signals should run in the linear phase of calibration; overnight will work if there is a very slow reaction f.i. if reaction was cooled
-The Bearer-
QUOTE (immuno9 @ Oct 3 2007, 05:19 PM)
My last few took overnight...are these result bogus? Thanks!
I am doing competitive ELISA, so no standards...
-immuno9-